Strain Name:
C57BL/6J-MtgxR6274Btlr/Mmmh
Stock Number:
044444-MU
Citation ID:
RRID:MMRRC_044444-MU
Other Names:
R6274 (G1)
Major Collection:

Strain Information

Notch3
Name: notch 3
Synonyms: hpbk, N3
Type: Gene
Species: Mouse
Chromosome: 17
NCBI: 18131
HGNC: HGNC:7883
Homologene: 376
Myh7
Name: myosin, heavy polypeptide 7, cardiac muscle, beta
Synonyms: Myhc-b, Myhcb, MYH-beta/slow, beta-MHC, B-MHC, MyHC-I, betaMHC
Type: Gene
Species: Mouse
Chromosome: 14
NCBI: 140781
HGNC: HGNC:7577
Homologene: 68044
Bpifb3
Name: BPI fold containing family B, member 3
Synonyms: Rya3
Type: Gene
Species: Mouse
Chromosome: 2
NCBI: 378700
Homologene: 18807
Hdac1
Name: histone deacetylase 1
Synonyms: RPD3, HD1, MommeD5
Type: Gene
Species: Mouse
Chromosome: 4
NCBI: 433759
HGNC: HGNC:4852
Homologene: 68426
Ano1
Name: anoctamin 1, calcium activated chloride channel
Synonyms: Tmem16a
Type: Gene
Species: Mouse
Chromosome: 7
NCBI: 101772
Homologene: 75079
Cep290
Name: centrosomal protein 290
Synonyms: Nphp6, b2b1454Clo, b2b1752Clo, Kiaa
Type: Gene
Species: Mouse
Chromosome: 10
NCBI: 216274
VEGA: 10
Homologene: 77213
Uaca
Name: uveal autoantigen with coiled-coil domains and ankyrin repeats
Synonyms: nucling, 2700059D02Rik
Type: Gene
Species: Mouse
Chromosome: 9
NCBI: 72565
VEGA: 9
Homologene: 74297
Ppp1r12a
Name: protein phosphatase 1, regulatory subunit 12A
Synonyms: 1200015F06Rik, Mypt1, D10Ertd625e, 5730577I22Rik
Type: Gene
Species: Mouse
Chromosome: 10
NCBI: 17931
VEGA: 10
HGNC: HGNC:7618
Homologene: 1855
Polr1a
Name: polymerase (RNA) I polypeptide A
Synonyms: mRPA1, RPA194, 194kDa, 3010014K16Rik, Rpo1-4, 2900087K15Rik
Type: Gene
Species: Mouse
Chromosome: 6
NCBI: 20019
Homologene: 7033
Ank3
Name: ankyrin 3, epithelial
Synonyms: Ank-3, Ankyrin-3, AnkG, 2900054D09Rik, Ankyrin-G
Type: Gene
Species: Mouse
Chromosome: 10
NCBI: 11735
HGNC: HGNC:494
Homologene: 56908
Osbpl11
Name: oxysterol binding protein-like 11
Synonyms: ORP-11
Type: Gene
Species: Mouse
Chromosome: 16
NCBI: 106326
Homologene: 23385
Ppm1g
Name: protein phosphatase 1G (formerly 2C), magnesium-dependent, gamma isoform
Synonyms: Fin13
Type: Gene
Species: Mouse
Chromosome: 5
NCBI: 14208
HGNC: HGNC:9278
Homologene: 31106
Clock
Name: clock circadian regulator
Synonyms: 5330400M04Rik, KAT13D, bHLHe8
Type: Gene
Species: Mouse
Chromosome: 5
NCBI: 12753
HGNC: HGNC:2082
Homologene: 3603
Grid2ip
Name: glutamate receptor, ionotropic, delta 2 (Grid2) interacting protein 1
Synonyms: delphilin
Type: Gene
Species: Mouse
Chromosome: 5
NCBI: 170935
Homologene: 15781
Ikzf1
Name: IKAROS family zinc finger 1
Synonyms: LyF-1, Ikaros, 5832432G11Rik, Zfpn1a1
Type: Gene
Species: Mouse
Chromosome: 11
NCBI: 22778
Homologene: 55948
Htt
Name: huntingtin
Synonyms: huntingtin, HD, htt, IT15, C430023I11Rik, Hdh
Type: Gene
Species: Mouse
Chromosome: 5
NCBI: 15194
HGNC: HGNC:4851
Homologene: 1593
Nktr
Name: natural killer tumor recognition sequence
Synonyms: D9Wsu172e, 5330401F18Rik
Type: Gene
Species: Mouse
Chromosome: 9
NCBI: 18087
HGNC: HGNC:7833
Homologene: 122148
Ice1
Name: interactor of little elongation complex ELL subunit 1
Synonyms: BC018507
Type: Gene
Species: Mouse
Chromosome: 13
NCBI: 218333
VEGA: 13
Homologene: 18902
Tbc1d2
Name: TBC1 domain family, member 2
Synonyms: LOC381605, PARIS-1, PARIS1, A630005A06Rik
Type: Gene
Species: Mouse
Chromosome: 4
NCBI: 381605
Homologene: 10190
Sez6l
Name: seizure related 6 homolog like
Synonyms: Acig1
Type: Gene
Species: Mouse
Chromosome: 5
NCBI: 56747
Homologene: 10895
Krt74
Name: keratin 74
Synonyms: Kb37
Type: Gene
Species: Mouse
Chromosome: 15
NCBI: 406222
VEGA: 15
Dock10
Name: dedicator of cytokinesis 10
Synonyms: Jr5, Jr4, ZIZ3, 9330153B10Rik, A630054M16Rik, Zizimin3
Type: Gene
Species: Mouse
Chromosome: 1
NCBI: 210293
Homologene: 45952
Nlrp2
Name: NLR family, pyrin domain containing 2
Synonyms: PYPAF2, E330007A02Rik, Nalp2, Pan1, Nbs1
Type: Gene
Species: Mouse
Chromosome: 7
NCBI: 232827
Homologene: 56789
Cacna1s
Name: calcium channel, voltage-dependent, L type, alpha 1S subunit
Synonyms: Cchl1a3, fmd, mdg, sj, muscle dysgenesis, Cav1.1, DHPR alpha1s
Type: Gene
Species: Mouse
Chromosome: 1
NCBI: 12292
HGNC: HGNC:1397
Homologene: 37257
Fer1l4
Name: fer-1 like family member 4
Synonyms: 9130402C12Rik
Type: Gene
Species: Mouse
Chromosome: 2
NCBI: 74562
Homologene: 19075
Sclt1
Name: sodium channel and clathrin linker 1
Synonyms: 4931421F20Rik, 2610207F23Rik
Type: Gene
Species: Mouse
Chromosome: 3
NCBI: 67161
Homologene: 27031
Csmd3
Name: CUB and Sushi multiple domains 3
Synonyms: 4930500N14Rik
Type: Gene
Species: Mouse
Chromosome: 15
NCBI: 239420
Homologene: 65982
Pcsk6
Name: proprotein convertase subtilisin/kexin type 6
Synonyms: PACE4, SPC4, b2b2830Clo
Type: Gene
Species: Mouse
Chromosome: 7
NCBI: 18553
HGNC: HGNC:8569
Homologene: 20569
Serpinb1a
Name: serine (or cysteine) peptidase inhibitor, clade B, member 1a
Synonyms: LEI, 1190005M04Rik, ovalbumin, ELANH2, M/NEI, MNEI, EIA
Type: Gene
Species: Mouse
Chromosome: 13
NCBI: 66222
HGNC: HGNC:3311
Homologene: 133768
Fetub
Name: fetuin beta
Synonyms: 2310011O17Rik, D17980
Type: Gene
Species: Mouse
Chromosome: 16
NCBI: 59083
VEGA: 16
HGNC: HGNC:3658
Homologene: 8660
Wnk4
Name: WNK lysine deficient protein kinase 4
Synonyms: 2010002J11Rik, Prkwnk4
Type: Gene
Species: Mouse
Chromosome: 11
NCBI: 69847
Homologene: 13020
Greb1
Name: gene regulated by estrogen in breast cancer protein
Synonyms: 5730583K22Rik
Type: Gene
Species: Mouse
Chromosome: 12
NCBI: 268527
Homologene: 8780
Mmut
Name: methylmalonyl-Coenzyme A mutase
Synonyms: D230010K02Rik, Mut
Type: Gene
Species: Mouse
Chromosome: 17
NCBI: 17850
VEGA: 17
HGNC: HGNC:7526
Homologene: 20097
Nrap
Name: nebulin-related anchoring protein
Type: Gene
Species: Mouse
Chromosome: 19
NCBI: 18175
HGNC: HGNC:7988
Homologene: 4499
Plxnb1
Name: plexin B1
Synonyms: 2900002G15Rik
Type: Gene
Species: Mouse
Chromosome: 9
NCBI: 235611
HGNC: HGNC:9103
Homologene: 130508
Or10ak8
Name: olfactory receptor family 10 subfamily AK member 8
Synonyms: GA_x6K02T2QD9B-18619941-18620882, MOR259-2, Olfr1329
Type: Gene
Species: Mouse
Chromosome: 4
NCBI: 258214
Homologene: 79401
Sipa1l2
Name: signal-induced proliferation-associated 1 like 2
Type: Gene
Species: Mouse
Chromosome: 8
NCBI: 244668
Homologene: 18956
Prodh
Name: proline dehydrogenase
Synonyms: Pro1, Pro-1, Ym24d07
Type: Gene
Species: Mouse
Chromosome: 16
NCBI: 19125
HGNC: HGNC:9453
Homologene: 40764
Il3ra
Name: interleukin 3 receptor, alpha chain
Synonyms: IL-3 receptor alpha chain, CD123, SUT-1
Type: Gene
Species: Mouse
Chromosome: 14
NCBI: 16188
VEGA: 14
HGNC: HGNC:6012
Homologene: 48088
Gucy1b2
Name: guanylate cyclase 1, soluble, beta 2
Type: Gene
Species: Mouse
Chromosome: 14
NCBI: 239134
HGNC: HGNC:4686
Homologene: 136630
Kif21b
Name: kinesin family member 21B
Synonyms: N-5 kinesin, 2610511N21Rik
Type: Gene
Species: Mouse
Chromosome: 1
NCBI: 16565
Homologene: 56868
Usp9y
Name: ubiquitin specific peptidase 9, Y chromosome
Synonyms: Dffry, Fafl2
Type: Gene
Species: Mouse
Chromosome: Y
NCBI: 107868
Homologene: 68408
Uqcrc1
Name: ubiquinol-cytochrome c reductase core protein 1
Synonyms: 1110032G10Rik
Type: Gene
Species: Mouse
Chromosome: 9
NCBI: 22273
Homologene: 2525
Or5b99
Name: olfactory receptor family 5 subfamily B member 99
Synonyms: GA_x6K02T2RE5P-3328502-3329434, MOR202-1, Olfr1451
Type: Gene
Species: Mouse
Chromosome: 19
NCBI: 258700
HGNC: HGNC:8323
Homologene: 128082
Zfp326
Name: zinc finger protein 326
Synonyms: ZAN75, 5730470H14Rik
Type: Gene
Species: Mouse
Chromosome: 5
NCBI: 54367
Homologene: 10297
Plaat5
Name: phospholipase A and acyltransferase 5
Synonyms: 1700086E08Rik, 1700055A14Rik, 4921526K24Rik, iNAT, Hrasls5
Type: Gene
Species: Mouse
Chromosome: 19
NCBI: 66727
Homologene: 12019
Cfb
Name: complement factor B
Synonyms: B, Factor B, alternative-complement pathway C3/C5 convertase, Bf, FB, H2-Bf
Type: Gene
Species: Mouse
Chromosome: 17
NCBI: 14962
HGNC: HGNC:1037
Homologene: 1292
Cers1
Name: ceramide synthase 1
Synonyms: Uog-1, CerS1, to, Lass1
Type: Gene
Species: Mouse
Chromosome: 8
NCBI: 93898
Homologene: 128762
Cdk5rap1
Name: CDK5 regulatory subunit associated protein 1
Synonyms: 2310066P17Rik
Type: Gene
Species: Mouse
Chromosome: 2
NCBI: 66971
Homologene: 9502
Bsnd
Name: barttin CLCNK type accessory beta subunit
Synonyms: Bartter syndrome, infantile, with sensorineural deafness (Barttin)
Type: Gene
Species: Mouse
Chromosome: 4
NCBI: 140475
Homologene: 14291
Araf
Name: Araf proto-oncogene, serine/threonine kinase
Synonyms: 1200013E08Rik, A-Raf, Araf1
Type: Gene
Species: Mouse
Chromosome: X
NCBI: 11836
HGNC: HGNC:646
Homologene: 1249
Sap18b
Name: Sin3-associated polypeptide 18B
Synonyms: Gm10094
Type: Gene
Species: Mouse
Chromosome: 8
NCBI: 100041953
Homologene: 4289
Krtap29-1
Name: keratin associated protein 29-1
Synonyms: Gm14195
Type: Gene
Species: Mouse
Chromosome: 11
NCBI: 100462664
Rilpl2
Name: Rab interacting lysosomal protein-like 2
Type: Gene
Species: Mouse
Chromosome: 5
NCBI: 80291
Homologene: 12723
Bcl2l10
Name: Bcl2-like 10
Synonyms: Boo, Diva
Type: Gene
Species: Mouse
Chromosome: 9
NCBI: 12049
VEGA: 9
HGNC: HGNC:993
Homologene: 8396
Genetic Alterations
ENU-induced transitions at the following base pair locations (GRCm38):
  • A to G, chromosome 1 at 80,538,823 bp
  • A to G, chromosome 1 at 136,089,045 bp
  • A to G, chromosome 1 at 136,149,418 bp
  • A to T, chromosome 2 at 153,929,323 bp
  • A to G, chromosome 2 at 154,368,241 bp
  • A to G, chromosome 2 at 156,029,268 bp
  • A to G, chromosome 3 at 41,629,516 bp
  • C to T, chromosome 4 at 46,629,912 bp
  • A to G, chromosome 4 at 106,486,635 bp
  • A to T, chromosome 4 at 118,917,230 bp
  • A to G, chromosome 4 at 129,519,109 bp
  • T to C, chromosome 5 at 31,206,406 bp
  • T to C, chromosome 5 at 34,852,087 bp
  • A to G, chromosome 5 at 76,237,153 bp
  • T to A, chromosome 5 at 105,905,980 bp
  • G to A, chromosome 5 at 112,475,365 bp
  • A to G, chromosome 5 at 124,469,848 bp
  • G to A, chromosome 5 at 143,380,429 bp
  • T to A, chromosome 6 at 71,954,890 bp
  • A to T, chromosome 7 at 5,317,555 bp
  • A to T, chromosome 7 at 66,033,844 bp
  • A to T, chromosome 7 at 144,618,863 bp
  • T to A, chromosome 8 at 70,331,077 bp
  • C to T, chromosome 8 at 95,825,541 bp
  • C to T, chromosome 8 at 125,469,872 bp
  • T to A, chromosome 9 at 60,850,291 bp
  • T to C, chromosome 9 at 75,351,072 bp
  • C to A, chromosome 9 at 108,942,156 bp
  • T to C, chromosome 9 at 109,112,141 bp
  • T to C, chromosome 9 at 121,731,565 bp
  • G to A, chromosome 10 at 70,002,565 bp
  • A to G, chromosome 10 at 100,530,207 bp
  • T to C, chromosome 10 at 108,260,890 bp
  • C to T, chromosome 11 at 11,768,961 bp
  • A to G, chromosome 11 at 51,271,921 bp
  • T to C, chromosome 11 at 99,978,983 bp
  • C to T, chromosome 11 at 101,265,431 bp
  • G to T, chromosome 12 at 16,735,151 bp
  • G to T, chromosome 13 at 32,842,866 bp
  • A to G, chromosome 13 at 70,594,839 bp
  • G to A, chromosome 14 at 14,350,180 bp
  • T to C, chromosome 14 at 54,979,486 bp
  • A to T, chromosome 14 at 62,415,939 bp
  • T to C, chromosome 15 at 47,621,437 bp
  • T to C, chromosome 15 at 101,763,437 bp
  • T to C, chromosome 16 at 18,081,058 bp
  • C to T, chromosome 16 at 22,932,331 bp
  • T to A, chromosome 16 at 33,227,056 bp
  • C to A, chromosome 17 at 32,147,290 bp
  • T to C, chromosome 17 at 34,862,093 bp
  • T to C, chromosome 17 at 40,956,245 bp
  • C to T, chromosome 19 at 7,637,466 bp
  • G to A, chromosome 19 at 12,999,870 bp
  • T to C, chromosome 19 at 56,361,721 bp
  • G to T, chromosome X at 20,860,100 bp
  • C to T, chromosome Y at 1,316,735 bp
Phenotype
G1 male heterozygous mice are viable and fertile. The G1 mice were not characterized prior to archiving.
Strain Development
C57BL/6J mice were injected with ENU, resulting in G0 mice that were bred back to C57BL/6J. Sperm was archived from G1 male mice that produced viable G2 mice.
Suggested Control Mice
Wild-type littermates or C57BL/6J mice
MMRRC Genetic QC Summary
The MMRRC Centers have developed a genetic QC pipeline using MiniMUGA array genotyping to provide additional information on strain backgrounds for MMRRC congenic and inbred strains. For more information on when data may be available, or to request genotyping for a strain of interest, please contact mmrrc@missouri.edu. Older strains may not have this information.
Donor
Bruce Beutler, M.D., University of Texas Southwestern Medical Center.
Primary Reference
MUTAGENETIX record for R6274 (G1). B. Beutler and colleagues, Center for the Genetics of Host Defense, UT Southwestern, Dallas, TX.

Colony and Husbandry Information

Colony Surveillance Program and Current Health Reports

Mice recovered from a cryo-archive will have health surveillance performed on recipient females. Health reports will be provided prior to shipment. If you require additional health status information, please email mmrrc@missouri.edu.
Coat Color
Black
MMRRC Breeding System
Random intra-strain mating
Breeding Scheme(s)
When maintaining a live colony, heterozygous mice may be bred together, bred with wild-type siblings, or bred with C57BL/6J inbred mice.
Overall Breeding Performance
Undetermined; during development, mating was done with heterozygous males; viability, fertility and other breeding statistics for heterozygous females and homozygotes are unverified, but presumably would be similar to the C57BL/6J background strain
Average litter size
Undetermined
Recommended wean age
3 weeks

Order Request Information

Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.

Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.

The donor or their institution limits the distribution to non-profit institutions only.

Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.

Click button to Request this one strain. (Use the MMRRC Catalog Search to request more than one strain.)
MMRRC Item # Description Distribution Fee / Unit (US $)
*Shipping & Handling not included*
Units Notes
044444-MU-RESUS Litter recovered from cryo-archive $5,475.00 / Non-Profit Litter Recovered litter4; additional fees for any special requests.
Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

1 The distribution fee covers the expense of rederiving mice from a live mouse; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

2 An aliquot contains a sufficient number of embryos (in one or more vials or straws and based on the transfer success rate of the MMRRC facility) to transfer into one to three recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.

3 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.

4 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.