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Strain Name:
C57BL/6J-MtgxR6274Btlr/Mmmh
Stock Number:
044444-MU
Citation ID:
RRID:MMRRC_044444-MU
Other Names:
R6274 (G1)
Major Collection:

Strain Information

Notch3
Name: notch 3
Synonyms: hpbk, N3
Type: Gene
Species: Mouse
Chromosome: 17
NCBI: 18131
HGNC: HGNC:7883
Homologene: 376
Myh7
Name: myosin, heavy polypeptide 7, cardiac muscle, beta
Synonyms: Myhc-b, Myhcb, beta-MHC, B-MHC, MYH-beta/slow, MyHC-I, betaMHC
Type: Gene
Species: Mouse
Chromosome: 14
NCBI: 140781
HGNC: HGNC:7577
Homologene: 68044
Bpifb3
Name: BPI fold containing family B, member 3
Synonyms: Rya3
Type: Gene
Species: Mouse
Chromosome: 2
NCBI: 378700
Homologene: 18807
Hdac1
Name: histone deacetylase 1
Synonyms: RPD3, HD1, MommeD5
Type: Gene
Species: Mouse
Chromosome: 4
NCBI: 433759
HGNC: HGNC:4852
Homologene: 68426
Ano1
Name: anoctamin 1, calcium activated chloride channel
Synonyms: Tmem16a
Type: Gene
Species: Mouse
Chromosome: 7
NCBI: 101772
Homologene: 75079
Cep290
Name: centrosomal protein 290
Synonyms: Nphp6, b2b1454Clo, b2b1752Clo, Kiaa
Type: Gene
Species: Mouse
Chromosome: 10
NCBI: 216274
VEGA: 10
Homologene: 77213
Uaca
Name: uveal autoantigen with coiled-coil domains and ankyrin repeats
Synonyms: nucling, 2700059D02Rik
Type: Gene
Species: Mouse
Chromosome: 9
NCBI: 72565
VEGA: 9
Homologene: 74297
Genetic Alterations
ENU-induced transitions at the following base pair locations (GRCm38):
  • A to G, chromosome 1 at 80,538,823 bp
  • A to G, chromosome 1 at 136,089,045 bp
  • A to G, chromosome 1 at 136,149,418 bp
  • A to T, chromosome 2 at 153,929,323 bp
  • A to G, chromosome 2 at 154,368,241 bp
  • A to G, chromosome 2 at 156,029,268 bp
  • A to G, chromosome 3 at 41,629,516 bp
  • C to T, chromosome 4 at 46,629,912 bp
  • A to G, chromosome 4 at 106,486,635 bp
  • A to T, chromosome 4 at 118,917,230 bp
  • A to G, chromosome 4 at 129,519,109 bp
  • T to C, chromosome 5 at 31,206,406 bp
  • T to C, chromosome 5 at 34,852,087 bp
  • A to G, chromosome 5 at 76,237,153 bp
  • T to A, chromosome 5 at 105,905,980 bp
  • G to A, chromosome 5 at 112,475,365 bp
  • A to G, chromosome 5 at 124,469,848 bp
  • G to A, chromosome 5 at 143,380,429 bp
  • T to A, chromosome 6 at 71,954,890 bp
  • A to T, chromosome 7 at 5,317,555 bp
  • A to T, chromosome 7 at 66,033,844 bp
  • A to T, chromosome 7 at 144,618,863 bp
  • T to A, chromosome 8 at 70,331,077 bp
  • C to T, chromosome 8 at 95,825,541 bp
  • C to T, chromosome 8 at 125,469,872 bp
  • T to A, chromosome 9 at 60,850,291 bp
  • T to C, chromosome 9 at 75,351,072 bp
  • C to A, chromosome 9 at 108,942,156 bp
  • T to C, chromosome 9 at 109,112,141 bp
  • T to C, chromosome 9 at 121,731,565 bp
  • G to A, chromosome 10 at 70,002,565 bp
  • A to G, chromosome 10 at 100,530,207 bp
  • T to C, chromosome 10 at 108,260,890 bp
  • C to T, chromosome 11 at 11,768,961 bp
  • A to G, chromosome 11 at 51,271,921 bp
  • T to C, chromosome 11 at 99,978,983 bp
  • C to T, chromosome 11 at 101,265,431 bp
  • G to T, chromosome 12 at 16,735,151 bp
  • G to T, chromosome 13 at 32,842,866 bp
  • A to G, chromosome 13 at 70,594,839 bp
  • G to A, chromosome 14 at 14,350,180 bp
  • T to C, chromosome 14 at 54,979,486 bp
  • A to T, chromosome 14 at 62,415,939 bp
  • T to C, chromosome 15 at 47,621,437 bp
  • T to C, chromosome 15 at 101,763,437 bp
  • T to C, chromosome 16 at 18,081,058 bp
  • C to T, chromosome 16 at 22,932,331 bp
  • T to A, chromosome 16 at 33,227,056 bp
  • C to A, chromosome 17 at 32,147,290 bp
  • T to C, chromosome 17 at 34,862,093 bp
  • T to C, chromosome 17 at 40,956,245 bp
  • C to T, chromosome 19 at 7,637,466 bp
  • G to A, chromosome 19 at 12,999,870 bp
  • T to C, chromosome 19 at 56,361,721 bp
  • G to T, chromosome X at 20,860,100 bp
  • C to T, chromosome Y at 1,316,735 bp
Phenotype
G1 male heterozygous mice are viable and fertile. The G1 mice were not characterized prior to archiving.
Strain GQC Summary
The MMRRC Centers have developed a Strain GQC pipeline using MiniMUGA array genotyping to provide additional information on strain backgrounds for MMRRC strains. For more information on whether data may be available, or to request genotyping for a strain of interest, please contact MMRRC_GeneticQC@med.unc.edu. Older strains may not have this information available.
Suggested Control Mice
Littermates of all relevant genotypes.
Donor
Bruce Beutler, M.D., University of Texas Southwestern Medical Center.
Primary Reference
MUTAGENETIX record for R6274 (G1). B. Beutler and colleagues, Center for the Genetics of Host Defense, UT Southwestern, Dallas, TX.
Strain Development
C57BL/6J mice were injected with ENU, resulting in G0 mice that were bred back to C57BL/6J. Sperm was archived from G1 male mice that produced viable G2 mice.


Disclaimer: If MMRRC Strain Genetic Quality Control (GQC; based on MiniMUGA genotyping and analysis) has been completed for this strain, the information might differ from the genetic background information provided by the submitter. MiniMUGA genetic analysis is done on a strain’s tissue samples taken when archived by or ordered from the assigned MMRRC Center.

Colony and Husbandry Information

Colony Surveillance Program and Current Health Reports

Mice recovered from a cryo-archive will have health surveillance performed on recipient females. Health reports will be provided prior to shipment. If you require additional health status information, please email mmrrc@missouri.edu.
Coat Color
Black
MMRRC Breeding System
Random intra-strain mating
Breeding Scheme(s)
When maintaining a live colony, heterozygous mice may be bred together, bred with wild-type siblings, or bred with C57BL/6J inbred mice.
Overall Breeding Performance
Undetermined; during development, mating was done with heterozygous males; viability, fertility and other breeding statistics for heterozygous females and homozygotes are unverified, but presumably would be similar to the C57BL/6J background strain
Average litter size
Undetermined
Recommended wean age
3 weeks

Order Request Information

Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.

Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.

The donor or their institution limits the distribution to non-profit institutions only.

Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.

Click button to Request this one strain. (Use the MMRRC Catalog Search to request more than one strain.)
MMRRC Item # Description Distribution Fee / Unit (US $)
*Shipping & Handling not included*
Units Notes
044444-MU-RESUS Litter recovered from cryo-archive $5,475.00 / Non-Profit Litter Recovered litter4; additional fees for any special requests.
Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

1 The distribution fee covers the expense of rederiving mice from a live mouse; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

2 An aliquot contains a sufficient number of embryos (in one or more vials or straws and based on the transfer success rate of the MMRRC facility) to transfer into one to three recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.

3 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.

4 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.