Strain Name:
C57BL/6J-MtgxR6324Btlr/Mmmh
Stock Number:
044478-MU
Citation ID:
RRID:MMRRC_044478-MU
Other Names:
R6324 (G1)
Major Collection:

Strain Information

Cr1l
Name: complement C3b/C4b receptor 1 like
Synonyms: Crry, mCRY
Type: Gene
Species: Mouse
Chromosome: 1
NCBI: 12946
Homologene: 128275
Enah
Name: ENAH actin regulator
Synonyms: Mena, Ndpp1
Type: Gene
Species: Mouse
Chromosome: 1
NCBI: 13800
Homologene: 134005
Rapgef2
Name: Rap guanine nucleotide exchange factor (GEF) 2
Synonyms: nRapGEP, CNRasGEF, 5830453M24Rik, RA-GEF-1, Pdzgef1
Type: Gene
Species: Mouse
Chromosome: 3
NCBI: 76089
Homologene: 35477
Ube2o
Name: ubiquitin-conjugating enzyme E2O
Synonyms: B230113M03Rik
Type: Gene
Species: Mouse
Chromosome: 11
NCBI: 217342
Homologene: 11113
Fmn2
Name: formin 2
Type: Gene
Species: Mouse
Chromosome: 1
NCBI: 54418
Homologene: 137334
Ptprg
Name: protein tyrosine phosphatase receptor type G
Synonyms: 5430405N12Rik, RPTPgamma
Type: Gene
Species: Mouse
Chromosome: 14
NCBI: 19270
HGNC: HGNC:9671
Homologene: 2129
Sorbs1
Name: sorbin and SH3 domain containing 1
Synonyms: 2310065E01Rik, 9530001P15Rik, Ponsin, Sh3d5, CAP, c-Cbl-associated protein
Type: Gene
Species: Mouse
Chromosome: 19
NCBI: 20411
VEGA: 19
Homologene: 83252
Nufip2
Name: nuclear FMR1 interacting protein 2
Synonyms: 1110001M19Rik, 9530056D24Rik
Type: Gene
Species: Mouse
Chromosome: 11
NCBI: 68564
Homologene: 10808
Phkb
Name: phosphorylase kinase beta
Type: Gene
Species: Mouse
Chromosome: 8
NCBI: 102093
HGNC: HGNC:8927
Homologene: 247
Rsf1
Name: remodeling and spacing factor 1
Synonyms: 4832420A03Rik, XAP8, Hbxap, C030033M12Rik, p325
Type: Gene
Species: Mouse
Chromosome: 7
NCBI: 233532
Homologene: 41142
Focad
Name: focadhesin
Synonyms: BC057079
Type: Gene
Species: Mouse
Chromosome: 4
NCBI: 230393
Homologene: 9842
Ptcd3
Name: pentatricopeptide repeat domain 3
Synonyms: 2610034F17Rik, 2810422B04Rik
Type: Gene
Species: Mouse
Chromosome: 6
NCBI: 69956
Homologene: 41211
Fam171b
Name: family with sequence similarity 171, member B
Synonyms: D430039N05Rik
Type: Gene
Species: Mouse
Chromosome: 2
NCBI: 241520
Homologene: 18462
Frem1
Name: Fras1 related extracellular matrix protein 1
Synonyms: eyes2, heb, eyem02Jus, crf11, QBRICK
Type: Gene
Species: Mouse
Chromosome: 4
NCBI: 329872
Homologene: 27049
Atp13a3
Name: ATPase type 13A3
Synonyms: LOC385637, LOC224088, LOC224087
Type: Gene
Species: Mouse
Chromosome: 16
NCBI: 224088
Homologene: 23455
Gpn3
Name: GPN-loop GTPase 3
Synonyms: Atpbd1c, A930018B01Rik, D5Ertd708e
Type: Gene
Species: Mouse
Chromosome: 5
NCBI: 68080
Homologene: 6487
Dazap1
Name: DAZ associated protein 1
Synonyms: 2410042M16Rik, mPrrp
Type: Gene
Species: Mouse
Chromosome: 10
NCBI: 70248
HGNC: HGNC:2683
Homologene: 134440
Slc38a9
Name: solute carrier family 38, member 9
Synonyms: 9430067K09Rik, 6720411P22Rik, 4833412L08Rik, 9130023D20Rik
Type: Gene
Species: Mouse
Chromosome: 13
NCBI: 268706
VEGA: 13
Homologene: 18139
Eif3j1
Name: eukaryotic translation initiation factor 3, subunit J1
Synonyms: eIF3-p35, eIF3-alpha, 1810016I04Rik, Eif3s1, 2700079K05Rik, Eif3j
Type: Gene
Species: Mouse
Chromosome: 2
NCBI: 78655
HGNC: HGNC:3270
Homologene: 37845
Rfx7
Name: regulatory factor X, 7
Synonyms: 2510005N23Rik, D130086K05Rik, Rfxdc2, 9930116O05Rik
Type: Gene
Species: Mouse
Chromosome: 9
NCBI: 319758
Homologene: 11275
Baz2b
Name: bromodomain adjacent to zinc finger domain, 2B
Synonyms: D2Ertd794e, 5830435C13Rik
Type: Gene
Species: Mouse
Chromosome: 2
NCBI: 407823
HGNC: HGNC:963
Homologene: 8394
Nkx2-5
Name: NK2 homeobox 5
Synonyms: Csx, Nkx-2.5, Nkx2.5, tinman
Type: Gene
Species: Mouse
Chromosome: 17
NCBI: 18091
HGNC: HGNC:2488
Homologene: 3230
Dchs1
Name: dachsous cadherin related 1
Synonyms: 3110041P15Rik, C130033F22Rik
Type: Gene
Species: Mouse
Chromosome: 7
NCBI: 233651
Homologene: 2771
Dock10
Name: dedicator of cytokinesis 10
Synonyms: 9330153B10Rik, A630054M16Rik, ZIZ3, Jr4, Jr5, Zizimin3
Type: Gene
Species: Mouse
Chromosome: 1
NCBI: 210293
Homologene: 45952
Nalcn
Name: sodium leak channel, non-selective
Synonyms: Vgcnl1, A530023G15Rik
Type: Gene
Species: Mouse
Chromosome: 14
NCBI: 338370
VEGA: 14
Homologene: 21832
Tnn
Name: tenascin N
Synonyms: Tnw, tenascin-W
Type: Gene
Species: Mouse
Chromosome: 1
NCBI: 329278
Homologene: 18634
Vmn2r15
Name: vomeronasal 2, receptor 15
Synonyms: EG211223
Type: Gene
Species: Mouse
Chromosome: 5
NCBI: 211223
Homologene: 129606
Vmn2r70
Name: vomeronasal 2, receptor 70
Synonyms: EG620835
Type: Gene
Species: Mouse
Chromosome: 7
NCBI: 670940
Homologene: 115466
Gpr158
Name: G protein-coupled receptor 158
Synonyms: 5330427M13Rik
Type: Gene
Species: Mouse
Chromosome: 2
NCBI: 241263
Homologene: 19381
Ccdc27
Name: coiled-coil domain containing 27
Synonyms: LOC381580
Type: Gene
Species: Mouse
Chromosome: 4
NCBI: 381580
Homologene: 17596
Or5al1
Name: olfactory receptor family 5 subfamily AL member 1
Synonyms: MOR185-10, GA_x6K02T2Q125-47629317-47628376, Olfr1042
Type: Gene
Species: Mouse
Chromosome: 2
NCBI: 257941
Homologene: 133665
Arhgef4
Name: Rho guanine nucleotide exchange factor 4
Synonyms: Asef, 9330140K16Rik, C230030N03Rik
Type: Gene
Species: Mouse
Chromosome: 1
NCBI: 226970
HGNC: HGNC:684
Homologene: 49414
Mybpc1
Name: myosin binding protein C, slow-type
Synonyms: 8030451F13Rik, Slow-type C-protein
Type: Gene
Species: Mouse
Chromosome: 10
NCBI: 109272
HGNC: HGNC:7549
Homologene: 1846
Vmn2r108
Name: vomeronasal 2, receptor 108
Synonyms: EG627805
Type: Gene
Species: Mouse
Chromosome: 17
NCBI: 627805
Homologene: 129678
Atp1a2
Name: ATPase, Na+/K+ transporting, alpha 2 polypeptide
Synonyms: Atpa-3
Type: Gene
Species: Mouse
Chromosome: 1
NCBI: 98660
HGNC: HGNC:800
Homologene: 47947
Synj1
Name: synaptojanin 1
Synonyms: A930006D20Rik
Type: Gene
Species: Mouse
Chromosome: 16
NCBI: 104015
Homologene: 48252
Zfp11
Name: zinc finger protein 11
Synonyms: Zfp-11, Krox-6
Type: Gene
Species: Mouse
Chromosome: 5
NCBI: 22648
Homologene: 87241
Gstp2
Name: glutathione S-transferase, pi 2
Synonyms: Gst-3, Gst3, GSTpiA, Gst p-2
Type: Gene
Species: Mouse
Chromosome: 19
NCBI: 14869
HGNC: HGNC:4638
Homologene: 660
Vmn1r181
Name: vomeronasal 1 receptor 181
Synonyms: V1rd20
Type: Gene
Species: Mouse
Chromosome: 7
NCBI: 404289
Homologene: 104166
Plch1
Name: phospholipase C, eta 1
Synonyms: Plcl3, PLCeta1
Type: Gene
Species: Mouse
Chromosome: 3
NCBI: 269437
Homologene: 88833
Or13p4
Name: olfactory receptor family 13 subfamily P member 4
Synonyms: GA_x6K02T2QD9B-18856980-18857927, Olfr1342, MOR258-3
Type: Gene
Species: Mouse
Chromosome: 4
NCBI: 258708
Homologene: 17357
Or5b109
Name: olfactory receptor family 5 subfamily B member 109
Synonyms: GA_x6K02T2RE5P-3560863-3561795, MOR202-29P, Olfr1463
Type: Gene
Species: Mouse
Chromosome: 19
NCBI: 258120
HGNC: HGNC:8324
Homologene: 133886
Gm5592
Name: predicted gene 5592
Type: Gene
Species: Mouse
Chromosome: 7
NCBI: 434172
Homologene: 45597
Odad1
Name: outer dynein arm docking complex subunit 1
Synonyms: Ccdc114
Type: Gene
Species: Mouse
Chromosome: 7
NCBI: 211535
Homologene: 128610
Ms4a19
Name: membrane-spanning 4-domains, subfamily A, member 19
Synonyms: 1700025F22Rik
Type: Gene
Species: Mouse
Chromosome: 19
NCBI: 69416
Homologene: 137380
Loxl4
Name: lysyl oxidase-like 4
Synonyms: 4833426I20Rik
Type: Gene
Species: Mouse
Chromosome: 19
NCBI: 67573
Homologene: 12977
Prl7b1
Name: prolactin family 7, subfamily b, member 1
Synonyms: Prlpn, 1600014J19Rik, PLP-N
Type: Gene
Species: Mouse
Chromosome: 13
NCBI: 75596
Homologene: 17809
Lin7a
Name: lin-7 homolog A, crumbs cell polarity complex component
Synonyms: TIP-33, LIN-7A, MALS-1, Veli
Type: Gene
Species: Mouse
Chromosome: 10
NCBI: 108030
VEGA: 10
Homologene: 20976
Prop1
Name: paired like homeodomain factor 1
Synonyms: prophet of Pit-1, Prop-1, prophet of Pit1
Type: Gene
Species: Mouse
Chromosome: 11
NCBI: 19127
HGNC: HGNC:9455
Homologene: 4558
Gm3404
Name: predicted gene 3404
Type: Gene
Species: Mouse
Chromosome: 5
NCBI: 100041554
Homologene: 86127
Trbv19
Name: T cell receptor beta, variable 19
Synonyms: Gm16780, Tcrb-V6
Type: Gene
Species: Mouse
Chromosome: 6
NCBI: 100124684
Genetic Alterations
ENU-induced transitions at the following base pair locations (GRCm38):
  • G to A, chromosome 1 at 34,723,477 bp
  • G to A, chromosome 1 at 80,505,176 bp
  • T to A, chromosome 1 at 160,145,204 bp
  • C to G, chromosome 1 at 172,289,336 bp
  • A to T, chromosome 1 at 174,612,553 bp
  • A to G, chromosome 1 at 181,918,571 bp
  • A to G, chromosome 1 at 195,111,122 bp
  • A to G, chromosome 2 at 21,810,554 bp
  • A to G, chromosome 2 at 59,906,948 bp
  • A to T, chromosome 2 at 83,879,264 bp
  • C to T, chromosome 2 at 86,159,456 bp
  • A to G, chromosome 2 at 122,041,178 bp
  • C to T, chromosome 3 at 63,781,390 bp
  • C to T, chromosome 3 at 79,079,132 bp
  • T to C, chromosome 4 at 82,983,337 bp
  • C to T, chromosome 4 at 88,401,068 bp
  • G to A, chromosome 4 at 118,690,531 bp
  • A to T, chromosome 4 at 154,036,191 bp
  • A to G, chromosome 5 at 109,286,271 bp
  • C to T, chromosome 5 at 122,372,575 bp
  • C to T, chromosome 5 at 129,656,523 bp
  • A to T, chromosome 5 at 146,528,107 bp
  • G to A, chromosome 6 at 41,178,758 bp
  • C to T, chromosome 6 at 71,885,327 bp
  • G to A, chromosome 7 at 23,984,758 bp
  • A to C, chromosome 7 at 41,286,535 bp
  • A to G, chromosome 7 at 45,941,710 bp
  • G to A, chromosome 7 at 85,558,879 bp
  • CG to CGACGGCGGTG, chromosome 7 at 97,579,908 bp
  • G to T, chromosome 7 at 105,764,938 bp
  • A to G, chromosome 8 at 86,018,542 bp
  • C to A, chromosome 9 at 72,618,414 bp
  • A to G, chromosome 10 at 80,277,660 bp
  • A to G, chromosome 10 at 88,568,619 bp
  • T to A, chromosome 10 at 107,380,215 bp
  • G to A, chromosome 11 at 50,952,199 bp
  • A to G, chromosome 11 at 77,691,661 bp
  • A to T, chromosome 11 at 116,539,359 bp
  • A to T, chromosome 13 at 27,602,895 bp
  • A to G, chromosome 13 at 112,726,100 bp
  • T to A, chromosome 14 at 12,226,314 bp
  • A to G, chromosome 14 at 123,409,749 bp
  • A to G, chromosome 16 at 30,332,285 bp
  • A to T, chromosome 16 at 90,938,630 bp
  • A to T, chromosome 17 at 20,471,715 bp
  • G to C, chromosome 17 at 26,841,121 bp
  • A to T, chromosome 19 at 4,040,499 bp
  • T to A, chromosome 19 at 11,163,447 bp
  • A to G, chromosome 19 at 13,235,104 bp
  • T to C, chromosome 19 at 40,321,819 bp
  • G to A, chromosome 19 at 42,595,378 bp
Phenotype
G1 male heterozygous mice are viable and fertile. The G1 mice were not characterized prior to archiving.
Strain Development
C57BL/6J mice were injected with ENU, resulting in G0 mice that were bred back to C57BL/6J. Sperm was archived from G1 male mice that produced viable G2 mice.
Suggested Control Mice
Wild-type littermates or C57BL/6J mice
MMRRC Genetic QC Summary
The MMRRC Centers have developed a genetic QC pipeline using MiniMUGA array genotyping to provide additional information on strain backgrounds for MMRRC congenic and inbred strains. For more information on when data may be available, or to request genotyping for a strain of interest, please contact mmrrc@missouri.edu. Older strains may not have this information.
Donor
Bruce Beutler, M.D., University of Texas Southwestern Medical Center.
Primary Reference
MUTAGENETIX record for R6324 (G1). B. Beutler and colleagues, Center for the Genetics of Host Defense, UT Southwestern, Dallas, TX.

Colony and Husbandry Information

Colony Surveillance Program and Current Health Reports

Mice recovered from a cryo-archive will have health surveillance performed on recipient females. Health reports will be provided prior to shipment. If you require additional health status information, please email mmrrc@missouri.edu.
Coat Color
Black
MMRRC Breeding System
Random intra-strain mating
Breeding Scheme(s)
When maintaining a live colony, heterozygous mice may be bred together, bred with wild-type siblings, or bred with C57BL/6J inbred mice.
Overall Breeding Performance
Undetermined; during development, mating was done with heterozygous males; viability, fertility and other breeding statistics for heterozygous females and homozygotes are unverified, but presumably would be similar to the C57BL/6J background strain
Average litter size
Undetermined
Recommended wean age
3 weeks

Order Request Information

Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.

Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.

The donor or their institution limits the distribution to non-profit institutions only.

Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.

Click button to Request this one strain. (Use the MMRRC Catalog Search to request more than one strain.)
MMRRC Item # Description Distribution Fee / Unit (US $)
*Shipping & Handling not included*
Units Notes
044478-MU-RESUS Litter recovered from cryo-archive $5,475.00 / Non-Profit Litter Recovered litter4; additional fees for any special requests.
Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

1 The distribution fee covers the expense of rederiving mice from a live mouse; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

2 An aliquot contains a sufficient number of embryos (in one or more vials or straws and based on the transfer success rate of the MMRRC facility) to transfer into one to three recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.

3 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.

4 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.