Strain Name:
C57BL/6J-MtgxR6325Btlr/Mmmh
Stock Number:
044479-MU
Citation ID:
RRID:MMRRC_044479-MU
Other Names:
R6325 (G1)
Major Collection:

Strain Information

Gpd2
Name: glycerol phosphate dehydrogenase 2, mitochondrial
Synonyms: Gdm1
Type: Gene
Species: Mouse
Chromosome: 2
NCBI: 14571
HGNC: HGNC:4456
Homologene: 352
Grp
Name: gastrin releasing peptide
Synonyms: BLP
Type: Gene
Species: Mouse
Chromosome: 18
NCBI: 225642
HGNC: HGNC:4605
Homologene: 1580
Fbrsl1
Name: fibrosin-like 1
Synonyms: 2410025L10Rik, Gm29766, LOC381668
Type: Gene
Species: Mouse
Chromosome: 5
NCBI: 381668
Homologene: 45966
Dip2b
Name: disco interacting protein 2 homolog B
Type: Gene
Species: Mouse
Chromosome: 15
NCBI: 239667
Homologene: 72227
Zfp462
Name: zinc finger protein 462
Synonyms: 9430078C22Rik, Gt4-2, Zfpip
Type: Gene
Species: Mouse
Chromosome: 4
NCBI: 242466
Homologene: 41430
Cct8
Name: chaperonin containing TCP1 subunit 8
Synonyms: Tcpq, Cctq
Type: Gene
Species: Mouse
Chromosome: 16
NCBI: 12469
HGNC: HGNC:1623
Homologene: 4802
Dnajc14
Name: DnaJ heat shock protein family (Hsp40) member C14
Synonyms: DRIP78, LIP6, HDJ3, 5730551F12Rik
Type: Gene
Species: Mouse
Chromosome: 10
NCBI: 74330
VEGA: 10
Homologene: 12553
Hamp
Name: hepcidin antimicrobial peptide
Synonyms: Hepc, HEPC1
Type: Gene
Species: Mouse
Chromosome: 7
NCBI: 84506
Homologene: 10911
Itsn2
Name: intersectin 2
Synonyms: Sh3p18, Ese2, Sh3d1B, Eh domain, SH3 domain regulator of endocytosis 2
Type: Gene
Species: Mouse
Chromosome: 12
NCBI: 20403
VEGA: 12
HGNC: HGNC:6184
Homologene: 22627
Fancm
Name: Fanconi anemia, complementation group M
Synonyms: C730036B14Rik, D12Ertd364e
Type: Gene
Species: Mouse
Chromosome: 12
NCBI: 104806
VEGA: 12
Homologene: 35378
Nr4a2
Name: nuclear receptor subfamily 4, group A, member 2
Synonyms: RNR-1, Nurr1, HZF-3
Type: Gene
Species: Mouse
Chromosome: 2
NCBI: 18227
HGNC: HGNC:7981
Homologene: 4509
Zfp804a
Name: zinc finger protein 804A
Synonyms: C630007C17Rik
Type: Gene
Species: Mouse
Chromosome: 2
NCBI: 241514
Homologene: 18461
Taar2
Name: trace amine-associated receptor 2
Synonyms: Gpr58
Type: Gene
Species: Mouse
Chromosome: 10
NCBI: 209512
HGNC: HGNC:4514
Homologene: 110760
Vmn2r111
Name: vomeronasal 2, receptor 111
Synonyms: EG210876
Type: Gene
Species: Mouse
Chromosome: 17
NCBI: 210876
Homologene: 86604
Tnxb
Name: tenascin XB
Synonyms: Tnx, TN-MHC
Type: Gene
Species: Mouse
Chromosome: 17
NCBI: 81877
Homologene: 49589
Or2ak7
Name: olfactory receptor family 2 subfamily AK member 7
Synonyms: Olfr320, MOR285-5, MOR285-4, GA_x6K02T2NKPP-730312-729392, GA_x6K02T2NKPP-733777-732813, Olfr321-ps1
Type: Gene
Species: Mouse
Chromosome: 11
NCBI: 216783
Homologene: 133825
Arhgef4
Name: Rho guanine nucleotide exchange factor 4
Synonyms: 9330140K16Rik, C230030N03Rik, Asef
Type: Gene
Species: Mouse
Chromosome: 1
NCBI: 226970
HGNC: HGNC:684
Homologene: 49414
Prss1l
Name: serine protease 1 (trypsin 1) like
Synonyms: Gm5771
Type: Gene
Species: Mouse
Chromosome: 6
NCBI: 436523
Homologene: 88408
Smarca2
Name: SWI/SNF related, matrix associated, actin dependent regulator of chromatin, subfamily a, member 2
Synonyms: 2610209L14Rik, Snf2l2, brm
Type: Gene
Species: Mouse
Chromosome: 19
NCBI: 67155
Homologene: 2308
Cntn5
Name: contactin 5
Synonyms: NB-2, 6720426O10Rik, A830025P08Rik, LOC244683
Type: Gene
Species: Mouse
Chromosome: 9
NCBI: 244682
HGNC: HGNC:2175
Homologene: 28447
Ttll6
Name: tubulin tyrosine ligase-like family, member 6
Synonyms: D11Moh44e, 4932418K24Rik, D11Moh43e, t8130b59
Type: Gene
Species: Mouse
Chromosome: 11
NCBI: 237930
Homologene: 18229
Far2
Name: fatty acyl CoA reductase 2
Synonyms: Mlstd1
Type: Gene
Species: Mouse
Chromosome: 6
NCBI: 330450
Homologene: 69247
Plch1
Name: phospholipase C, eta 1
Synonyms: Plcl3, PLCeta1
Type: Gene
Species: Mouse
Chromosome: 3
NCBI: 269437
Homologene: 88833
Cyp11a1
Name: cytochrome P450, family 11, subfamily a, polypeptide 1
Synonyms: cholesterol side chain cleavage, D9Ertd411e, P450scc, Scc, Cyp11a, cscc
Type: Gene
Species: Mouse
Chromosome: 9
NCBI: 13070
HGNC: HGNC:2590
Homologene: 37347
Birc7
Name: baculoviral IAP repeat-containing 7
Synonyms: ML-IAP, Livin, KIAP
Type: Gene
Species: Mouse
Chromosome: 2
NCBI: 329581
Homologene: 51405
Acoxl
Name: acyl-Coenzyme A oxidase-like
Synonyms: 1200014P05Rik
Type: Gene
Species: Mouse
Chromosome: 2
NCBI: 74121
Homologene: 75083
Hecw1
Name: HECT, C2 and WW domain containing E3 ubiquitin protein ligase 1
Synonyms: NEDL1, E130207I19Rik
Type: Gene
Species: Mouse
Chromosome: 13
NCBI: 94253
VEGA: 13
Homologene: 9004
Tex47
Name: testis expressed 47
Synonyms: 4921511H03Rik
Type: Gene
Species: Mouse
Chromosome: 5
NCBI: 70920
Homologene: 41723
Pla2g2e
Name: phospholipase A2, group IIE
Synonyms: mGIIEsPLA2s
Type: Gene
Species: Mouse
Chromosome: 4
NCBI: 26970
Homologene: 8083
Ptpn20
Name: protein tyrosine phosphatase, non-receptor type 20
Synonyms: typ
Type: Gene
Species: Mouse
Chromosome: 14
NCBI: 19256
VEGA: 14
Homologene: 74905
Or2w1
Name: olfactory receptor family 2 subfamily W member 1
Synonyms: GA_x6K02T2QHY8-12114828-12113875, Olfr42, IA3, Olfr263-ps1, GA_x6K02T2N5E5-9379-8514, MOR256-61, Olfr263, MOR256-37P, MOR256-31
Type: Gene
Species: Mouse
Chromosome: 13
NCBI: 18341
HGNC: HGNC:8281
Homologene: 12791
Genetic Alterations
ENU-induced transitions at the following base pair locations (GRCm38):
  • G to A, chromosome 1 at 34,723,477 bp
  • A to G, chromosome 2 at 57,112,418 bp
  • T to C, chromosome 2 at 57,304,396 bp
  • A to T, chromosome 2 at 82,257,038 bp
  • T to C, chromosome 2 at 128,123,024 bp
  • G to A, chromosome 2 at 180,929,450 bp
  • C to T, chromosome 3 at 63,781,390 bp
  • C to T, chromosome 4 at 55,080,680 bp
  • T to C, chromosome 4 at 138,880,425 bp
  • A to T, chromosome 5 at 7,304,935 bp
  • A to G, chromosome 5 at 110,377,407 bp
  • T to C, chromosome 6 at 41,396,656 bp
  • T to C, chromosome 6 at 148,157,497 bp
  • A to C, chromosome 7 at 30,943,903 bp
  • T to C, chromosome 9 at 10,144,323 bp
  • A to G, chromosome 9 at 58,025,568 bp
  • A to T, chromosome 10 at 23,940,717 bp
  • A to G, chromosome 10 at 128,807,490 bp
  • T to A, chromosome 11 at 58,684,528 bp
  • T to C, chromosome 11 at 96,135,505 bp
  • T to A, chromosome 12 at 4,706,351 bp
  • T to A, chromosome 12 at 65,125,052 bp
  • T to A, chromosome 13 at 14,316,446 bp
  • A to T, chromosome 13 at 21,133,075 bp
  • A to T, chromosome 14 at 33,631,005 bp
  • T to C, chromosome 15 at 100,154,282 bp
  • A to C, chromosome 16 at 87,495,727 bp
  • T to C, chromosome 17 at 22,559,051 bp
  • T to A, chromosome 17 at 34,692,424 bp
  • T to C, chromosome 18 at 65,873,753 bp
  • T to A, chromosome 19 at 26,678,363 bp
Phenotype
G1 male heterozygous mice are viable and fertile. The G1 mice were not characterized prior to archiving.
Strain Development
C57BL/6J mice were injected with ENU, resulting in G0 mice that were bred back to C57BL/6J. Sperm was archived from G1 male mice that produced viable G2 mice.
Suggested Control Mice
Wild-type littermates or C57BL/6J mice
MMRRC Genetic QC Summary
The MMRRC Centers have developed a genetic QC pipeline using MiniMUGA array genotyping to provide additional information on strain backgrounds for MMRRC congenic and inbred strains. For more information on when data may be available, or to request genotyping for a strain of interest, please contact mmrrc@missouri.edu. Older strains may not have this information.
Donor
Bruce Beutler, M.D., University of Texas Southwestern Medical Center.
Primary Reference
MUTAGENETIX record for R6325 (G1). B. Beutler and colleagues, Center for the Genetics of Host Defense, UT Southwestern, Dallas, TX.

Colony and Husbandry Information

Colony Surveillance Program and Current Health Reports

Mice recovered from a cryo-archive will have health surveillance performed on recipient females. Health reports will be provided prior to shipment. If you require additional health status information, please email mmrrc@missouri.edu.
Coat Color
Black
MMRRC Breeding System
Random intra-strain mating
Breeding Scheme(s)
When maintaining a live colony, heterozygous mice may be bred together, bred with wild-type siblings, or bred with C57BL/6J inbred mice.
Overall Breeding Performance
Undetermined; during development, mating was done with heterozygous males; viability, fertility and other breeding statistics for heterozygous females and homozygotes are unverified, but presumably would be similar to the C57BL/6J background strain
Average litter size
Undetermined
Recommended wean age
3 weeks

Order Request Information

Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.

Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.

The donor or their institution limits the distribution to non-profit institutions only.

Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.

Click button to Request this one strain. (Use the MMRRC Catalog Search to request more than one strain.)
MMRRC Item # Description Distribution Fee / Unit (US $)
*Shipping & Handling not included*
Units Notes
044479-MU-RESUS Litter recovered from cryo-archive $5,475.00 / Non-Profit Litter Recovered litter4; additional fees for any special requests.
Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

1 The distribution fee covers the expense of rederiving mice from a live mouse; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

2 An aliquot contains a sufficient number of embryos (in one or more vials or straws and based on the transfer success rate of the MMRRC facility) to transfer into one to three recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.

3 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.

4 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.