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Strain Name:
C57BL/6J-MtgxR6328Btlr/Mmmh
Stock Number:
044482-MU
Citation ID:
RRID:MMRRC_044482-MU
Other Names:
R6328 (G1)
Major Collection:

Strain Information

Gulo
Name: gulonolactone (L-) oxidase
Synonyms: L-gulono-gamma-lactone oxidase, sfx
Type: Gene
Species: Mouse
Chromosome: 14
NCBI: 268756
VEGA: 14
HGNC: HGNC:4695
Homologene: 6566
Pcp2
Name: Purkinje cell protein 2 (L7)
Synonyms: L7, Pcp-2
Type: Gene
Species: Mouse
Chromosome: 8
NCBI: 18545
Homologene: 7464
Htr3b
Name: 5-hydroxytryptamine (serotonin) receptor 3B
Synonyms: 5-HT3 receptor subunit B, 5-HT3B
Type: Gene
Species: Mouse
Chromosome: 9
NCBI: 57014
VEGA: 9
HGNC: HGNC:5298
Homologene: 38131
Cd59a
Name: CD59a antigen
Synonyms: Cd59, protectin
Type: Gene
Species: Mouse
Chromosome: 2
NCBI: 12509
HGNC: HGNC:1689
Nudt5
Name: nudix hydrolase 5
Synonyms: nudix (nucleoside diphosphate linked moiety X)-type motif 5
Type: Gene
Species: Mouse
Chromosome: 2
NCBI: 53893
HGNC: HGNC:8052
Homologene: 5388
Rbm6
Name: RNA binding motif protein 6
Synonyms: NY-LU-12, g16, Def-3
Type: Gene
Species: Mouse
Chromosome: 9
NCBI: 19654
HGNC: HGNC:9903
Homologene: 31336
Genetic Alterations
ENU-induced transitions at the following base pair locations (GRCm38):
  • T to A, chromosome 1 at 37,911,113 bp
  • T to A, chromosome 1 at 86,854,431 bp
  • A to T, chromosome 1 at 131,180,668 bp
  • C to A, chromosome 1 at 138,113,678 bp
  • A to G, chromosome 1 at 138,287,832 bp
  • A to G, chromosome 1 at 169,987,065 bp
  • A to T, chromosome 2 at 4,590,698 bp
  • A to T, chromosome 2 at 5,864,437 bp
  • A to G, chromosome 2 at 29,174,462 bp
  • T to A, chromosome 2 at 66,273,316 bp
  • A to G, chromosome 2 at 91,942,635 bp
  • A to C, chromosome 2 at 104,110,758 bp
  • A to T, chromosome 2 at 111,945,394 bp
  • A to G, chromosome 2 at 158,635,344 bp
  • G to A, chromosome 2 at 158,785,176 bp
  • T to C, chromosome 3 at 88,486,506 bp
  • A to G, chromosome 3 at 142,569,058 bp
  • A to T, chromosome 5 at 114,670,511 bp
  • T to C, chromosome 6 at 13,602,711 bp
  • A to G, chromosome 6 at 52,746,709 bp
  • G to T, chromosome 6 at 69,900,505 bp
  • T to A, chromosome 6 at 125,224,918 bp
  • C to T, chromosome 6 at 141,932,450 bp
  • T to C, chromosome 7 at 24,105,556 bp
  • A to T, chromosome 7 at 103,597,866 bp
  • A to G, chromosome 7 at 133,717,774 bp
  • A to G, chromosome 7 at 139,099,818 bp
  • T to C, chromosome 8 at 3,624,887 bp
  • T to A, chromosome 8 at 63,928,311 bp
  • G to A, chromosome 8 at 123,962,017 bp
  • A to T, chromosome 9 at 7,165,717 bp
  • A to T, chromosome 9 at 40,874,223 bp
  • A to T, chromosome 9 at 48,947,633 bp
  • T to A, chromosome 9 at 49,416,071 bp
  • A to T, chromosome 9 at 107,787,259 bp
  • C to T, chromosome 10 at 76,614,378 bp
  • A to G, chromosome 11 at 11,937,905 bp
  • G to C, chromosome 11 at 95,039,402 bp
  • T to A, chromosome 11 at 113,793,755 bp
  • T to C, chromosome 11 at 120,347,760 bp
  • A to C, chromosome 12 at 30,258,014 bp
  • A to G, chromosome 12 at 40,180,657 bp
  • C to A, chromosome 12 at 74,266,693 bp
  • T to C, chromosome 13 at 14,247,620 bp
  • A to T, chromosome 13 at 38,197,006 bp
  • G to T, chromosome 13 at 55,508,092 bp
  • A to G, chromosome 13 at 64,436,599 bp
  • G to T, chromosome 14 at 66,002,631 bp
  • C to A, chromosome 14 at 75,962,597 bp
  • C to T, chromosome 14 at 76,111,054 bp
  • C to T, chromosome 16 at 8,602,436 bp
  • A to T, chromosome 16 at 30,336,235 bp
  • T to A, chromosome 17 at 36,813,944 bp
  • G to A, chromosome 18 at 74,616,993 bp
  • A to G, chromosome 18 at 78,028,964 bp
  • C to T, chromosome 19 at 6,849,038 bp
  • C to T, chromosome 19 at 9,007,148 bp
Phenotype
G1 male heterozygous mice are viable and fertile. The G1 mice were not characterized prior to archiving.
Strain GQC Summary
Gene Specific Genotyping:

To request gene-specific and other genotyping services for a strain, please contact the distribution MMRRC Center for more information.

Background Genetic Quality:

The MMRRC has developed a Genetic Quality Control pipeline using the MiniMUGA array to provide additional information to identify and validate genetic backgrounds of MMRRC strains. For more information on whether genetic background data is available, please contact MMRRC_GeneticQC@med.unc.edu. Note: that MiniMUGA genetic background data is not available on all strains, but can be ordered if desired.

Suggested Control Mice
Littermates of all relevant genotypes.
Donor
Bruce Beutler, M.D., University of Texas Southwestern Medical Center.
Primary Reference
MUTAGENETIX record for R6328 (G1). B. Beutler and colleagues, Center for the Genetics of Host Defense, UT Southwestern, Dallas, TX.
Strain Development
C57BL/6J mice were injected with ENU, resulting in G0 mice that were bred back to C57BL/6J. Sperm was archived from G1 male mice that produced viable G2 mice.


Disclaimer: If MMRRC Strain Genetic Quality Control (GQC; based on MiniMUGA genotyping and analysis) has been completed for this strain, the information might differ from the genetic background information provided by the submitter. MiniMUGA genetic analysis is done on a strain’s tissue samples taken when archived by or ordered from the assigned MMRRC Center.

Colony and Husbandry Information

Colony Surveillance Program and Current Health Reports

Mice recovered from a cryo-archive will have health surveillance performed on recipient females. Health reports will be provided prior to shipment. If you require additional health status information, please email mmrrc@missouri.edu.
Coat Color
Black
MMRRC Breeding System
Random intra-strain mating
Breeding Scheme(s)
When maintaining a live colony, heterozygous mice may be bred together, bred with wild-type siblings, or bred with C57BL/6J inbred mice.
Overall Breeding Performance
Undetermined; during development, mating was done with heterozygous males; viability, fertility and other breeding statistics for heterozygous females and homozygotes are unverified, but presumably would be similar to the C57BL/6J background strain
Average litter size
Undetermined
Recommended wean age
3 weeks

Order Information

Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.

Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.

The donor or their institution limits the distribution to non-profit institutions only.

Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.

Click button to Request this one strain. (Use the MMRRC Catalog Search to request more than one strain.)
MMRRC Item # Description Distribution Fee / Unit (US $)
*Shipping & Handling not included*
Units Notes
044482-MU-RESUS Litter recovered from cryo-archive $5,475.00 / Non-Profit Litter Recovered litter4; additional fees for any special requests.
Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

1 The distribution fee covers the expense of rederiving mice from a live mouse; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

2 An aliquot contains a sufficient number of embryos (in one or more vials or straws and based on the transfer success rate of the MMRRC facility) to transfer into one to three recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.

3 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.

4 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.