Strain Name:
C57BL/6J-MtgxR6333Btlr/Mmmh
Stock Number:
044487-MU
Citation ID:
RRID:MMRRC_044487-MU
Other Names:
R6333 (G1)
Major Collection:

Strain Information

Chrnb3
Name: cholinergic receptor, nicotinic, beta polypeptide 3
Synonyms: 5730417K16Rik, Acrb3
Type: Gene
Species: Mouse
Chromosome: 8
NCBI: 108043
HGNC: HGNC:1963
Homologene: 36035
Rai14
Name: retinoic acid induced 14
Synonyms: 1700008J19Rik, 1700020L11Rik, Ankycorbin, Norpeg
Type: Gene
Species: Mouse
Chromosome: 15
NCBI: 75646
VEGA: 15
Homologene: 9199
Pdss1
Name: prenyl (solanesyl) diphosphate synthase, subunit 1
Synonyms: Tprt, 2610203G20Rik, 2700031G06Rik, mSPS1
Type: Gene
Species: Mouse
Chromosome: 2
NCBI: 56075
Homologene: 5353
Brd7
Name: bromodomain containing 7
Synonyms: bromodomain protein 75 kDa, CELTIX1, BP75
Type: Gene
Species: Mouse
Chromosome: 8
NCBI: 26992
Homologene: 8085
Chmp2b
Name: charged multivesicular body protein 2B
Synonyms: chromatin modifying protein 2B, 1190006E07Rik
Type: Gene
Species: Mouse
Chromosome: 16
NCBI: 68942
VEGA: 16
Homologene: 8534
Ell
Name: elongation factor RNA polymerase II
Synonyms: eleven-nineteen lysine-rich leukemia gene, Men, Ell1
Type: Gene
Species: Mouse
Chromosome: 8
NCBI: 13716
Homologene: 4762
Ube4b
Name: ubiquitination factor E4B
Synonyms: 4933406G05Rik, Ufd2p, D4Bwg0973e, 4930551I19Rik, UFD2, UFD2a
Type: Gene
Species: Mouse
Chromosome: 4
NCBI: 63958
Homologene: 107623
Mark4
Name: MAP/microtubule affinity regulating kinase 4
Synonyms: 2410090P21Rik, Markl1
Type: Gene
Species: Mouse
Chromosome: 7
NCBI: 232944
Homologene: 57146
Nufip1
Name: nuclear FMR1 interacting protein 1
Type: Gene
Species: Mouse
Chromosome: 14
NCBI: 27275
VEGA: 14
HGNC: HGNC:8057
Homologene: 8216
Zwint
Name: ZW10 interactor
Synonyms: 2010007E07Rik, 2600001N01Rik, D10Ertd749e
Type: Gene
Species: Mouse
Chromosome: 10
NCBI: 52696
Homologene: 48496
Asb4
Name: ankyrin repeat and SOCS box-containing 4
Synonyms: 8430401O13Rik
Type: Gene
Species: Mouse
Chromosome: 6
NCBI: 65255
Homologene: 9392
Rnd2
Name: Rho family GTPase 2
Synonyms: Arhn, Rohn
Type: Gene
Species: Mouse
Chromosome: 11
NCBI: 11858
Homologene: 21123
Tent2
Name: terminal nucleotidyltransferase 2
Synonyms: 8030446C20Rik, Papd4
Type: Gene
Species: Mouse
Chromosome: 13
NCBI: 100715
VEGA: 13
Homologene: 44022
Tenm4
Name: teneurin transmembrane protein 4
Synonyms: Ten-m4, ELM2, Odz4, Doc4, l7Rn3, l(7)-3Rn
Type: Gene
Species: Mouse
Chromosome: 7
NCBI: 23966
Homologene: 8034
Shld2
Name: shieldin complex subunit 2
Synonyms: 3110001K24Rik, Fam35a
Type: Gene
Species: Mouse
Chromosome: 14
NCBI: 75698
VEGA: 14
Homologene: 56840
Prokr2
Name: prokineticin receptor 2
Synonyms: Gpr73l1, B830005M06Rik, EG-VEGRF2, PKR2, Gpcr73l1
Type: Gene
Species: Mouse
Chromosome: 2
NCBI: 246313
Homologene: 16368
Hspg2
Name: perlecan (heparan sulfate proteoglycan 2)
Synonyms: Plc, per, Pcn, perlecan
Type: Gene
Species: Mouse
Chromosome: 4
NCBI: 15530
HGNC: HGNC:5273
Homologene: 68473
Mme
Name: membrane metallo endopeptidase
Synonyms: neutral endopeptidase, 6030454K05Rik, NEP, neprilysin, CD10
Type: Gene
Species: Mouse
Chromosome: 3
NCBI: 17380
HGNC: HGNC:7154
Homologene: 5275
Bub1b
Name: BUB1B, mitotic checkpoint serine/threonine kinase
Synonyms: BUBR1
Type: Gene
Species: Mouse
Chromosome: 2
NCBI: 12236
HGNC: HGNC:1149
Homologene: 933
Tbc1d2
Name: TBC1 domain family, member 2
Synonyms: A630005A06Rik, LOC381605, PARIS-1, PARIS1
Type: Gene
Species: Mouse
Chromosome: 4
NCBI: 381605
Homologene: 10190
4931406C07Rik
Name: RIKEN cDNA 4931406C07 gene
Type: Gene
Species: Mouse
Chromosome: 9
NCBI: 70984
VEGA: 9
Homologene: 8531
Rnasek
Name: ribonuclease, RNase K
Synonyms: 2310033H11Rik, D11Bwg0434e
Type: Gene
Species: Mouse
Chromosome: 11
NCBI: 52898
Homologene: 45475
Clec4b2
Name: C-type lectin domain family 4, member b2
Synonyms: F830043G12Rik, mDCAR1
Type: Gene
Species: Mouse
Chromosome: 6
NCBI: 381809
Homologene: 128249
Rnf213
Name: ring finger protein 213
Synonyms: D11Ertd759e
Type: Gene
Species: Mouse
Chromosome: 11
NCBI: 672511
Homologene: 45439
Dnah3
Name: dynein, axonemal, heavy chain 3
Synonyms: Dnahc3
Type: Gene
Species: Mouse
Chromosome: 7
NCBI: 381917
HGNC: HGNC:2949
Homologene: 19674
Sctr
Name: secretin receptor
Synonyms: 6530402O03Rik
Type: Gene
Species: Mouse
Chromosome: 1
NCBI: 319229
Homologene: 68290
AI182371
Name: expressed sequence AI182371
Type: Gene
Species: Mouse
Chromosome: 2
NCBI: 98870
Homologene: 87268
Abhd18
Name: abhydrolase domain containing 18
Synonyms: 3110057O12Rik
Type: Gene
Species: Mouse
Chromosome: 3
NCBI: 269423
Homologene: 105457
Trmt1l
Name: tRNA methyltransferase 1 like
Synonyms: Trm1-like, 1190005F20Rik
Type: Gene
Species: Mouse
Chromosome: 1
NCBI: 98685
Homologene: 12801
Vmn2r104
Name: vomeronasal 2, receptor 104
Synonyms: V2r7
Type: Gene
Species: Mouse
Chromosome: 17
NCBI: 22313
Homologene: 129751
Cep85l
Name: centrosomal protein 85-like
Synonyms: Gm9766
Type: Gene
Species: Mouse
Chromosome: 10
NCBI: 100038725
VEGA: 10
Homologene: 52598
Defa34
Name: defensin, alpha, 34
Synonyms: Gm15315, ENSMUSG00000063206
Type: Gene
Species: Mouse
Chromosome: 8
NCBI: 100041952
Homologene: 113382
Acap1
Name: ArfGAP with coiled-coil, ankyrin repeat and PH domains 1
Synonyms: Centb1
Type: Gene
Species: Mouse
Chromosome: 11
NCBI: 216859
Homologene: 22835
Prss39
Name: serine protease 39
Synonyms: Tesp1
Type: Gene
Species: Mouse
Chromosome: 1
NCBI: 21755
Homologene: 84781
Ptar1
Name: protein prenyltransferase alpha subunit repeat containing 1
Synonyms: 2010013L17Rik, 4930428J16Rik, 1700084D09Rik
Type: Gene
Species: Mouse
Chromosome: 19
NCBI: 72351
VEGA: 19
Homologene: 34254
Fcgr4
Name: Fc receptor, IgG, low affinity IV
Synonyms: 4833442P21Rik, Fcgr3a, FcgammaRIV, CD16-2
Type: Gene
Species: Mouse
Chromosome: 1
NCBI: 246256
Homologene: 477
Sec14l5
Name: SEC14-like lipid binding 5
Type: Gene
Species: Mouse
Chromosome: 16
NCBI: 665119
VEGA: 16
Homologene: 96163
Sirpd
Name: signal regulatory protein delta
Synonyms: Gm9733
Type: Gene
Species: Mouse
Chromosome: 3
NCBI: 751864
Homologene: 135594
Hdac9
Name: histone deacetylase 9
Synonyms: HDRP, Mitr, Hdac7b, D030072B18Rik
Type: Gene
Species: Mouse
Chromosome: 12
NCBI: 79221
Homologene: 128578
Ppig
Name: peptidyl-prolyl isomerase G (cyclophilin G)
Synonyms: SRCyp, B230312B02Rik
Type: Gene
Species: Mouse
Chromosome: 2
NCBI: 228005
Homologene: 3520
Fbxw19
Name: F-box and WD-40 domain protein 19
Type: Gene
Species: Mouse
Chromosome: 9
NCBI: 235612
Homologene: 110776
Pcdh1
Name: protocadherin 1
Synonyms: 2010005A06Rik
Type: Gene
Species: Mouse
Chromosome: 18
NCBI: 75599
HGNC: HGNC:8655
Homologene: 12613
Esp36
Name: exocrine gland secreted peptide 36
Synonyms: Gm20408
Type: Gene
Species: Mouse
Chromosome: 17
NCBI: 100126765
Zfp3
Name: zinc finger protein 3
Synonyms: Zfp-3, Fnp-1
Type: Gene
Species: Mouse
Chromosome: 11
NCBI: 193043
Homologene: 27406
Plrg1
Name: pleiotropic regulator 1
Synonyms: Tango4
Type: Gene
Species: Mouse
Chromosome: 3
NCBI: 53317
HGNC: HGNC:9089
Homologene: 2004
Gm10110
Name: predicted gene 10110
Type: Gene
Species: Mouse
Chromosome: 14
Hsd3b6
Name: hydroxy-delta-5-steroid dehydrogenase, 3 beta- and steroid delta-isomerase 6
Synonyms: 3beta-HSD VI
Type: Gene
Species: Mouse
Chromosome: 3
NCBI: 15497
Homologene: 133013
Spag8
Name: sperm associated antigen 8
Type: Gene
Species: Mouse
Chromosome: 4
NCBI: 433700
Homologene: 8250
Dynlt2a1
Name: dynein light chain Tctex-type 2A1
Synonyms: Tctex4, Tcte3, LC2, Dynlt2, Tctex2, D17Leh117c, Tcte-3, Tctex-2, Tctex-4
Type: Gene
Species: Mouse
Chromosome: 17
NCBI: 21647
VEGA: 17
Homologene: 7971
Sash3
Name: SAM and SH3 domain containing 3
Synonyms: 1200013B08Rik, SLY1
Type: Gene
Species: Mouse
Chromosome: X
NCBI: 74131
Homologene: 10362
Genetic Alterations
ENU-induced transitions at the following base pair locations (GRCm38):
  • A to G, chromosome 1 at 34,500,069 bp
  • T to C, chromosome 1 at 120,056,452 bp
  • T to A, chromosome 1 at 151,453,934 bp
  • A to G, chromosome 1 at 171,029,269 bp
  • A to G, chromosome 2 at 22,901,766 bp
  • A to T, chromosome 2 at 35,085,269 bp
  • A to G, chromosome 2 at 52,258,263 bp
  • C to T, chromosome 2 at 69,749,558 bp
  • T to C, chromosome 2 at 118,598,463 bp
  • A to G, chromosome 2 at 132,373,978 bp
  • T to C, chromosome 3 at 15,320,611 bp
  • A to G, chromosome 3 at 40,933,783 bp
  • A to G, chromosome 3 at 63,341,961 bp
  • A to G, chromosome 3 at 83,056,795 bp
  • A to G, chromosome 3 at 98,806,224 bp
  • T to C, chromosome 4 at 43,653,186 bp
  • T to C, chromosome 4 at 46,620,736 bp
  • T to C, chromosome 4 at 137,561,955 bp
  • A to T, chromosome 4 at 138,871,397 bp
  • A to G, chromosome 4 at 149,348,037 bp
  • G to A, chromosome 6 at 5,423,597 bp
  • A to T, chromosome 6 at 123,200,678 bp
  • G to A, chromosome 7 at 19,443,283 bp
  • A to G, chromosome 7 at 96,774,124 bp
  • A to T, chromosome 7 at 120,054,633 bp
  • T to C, chromosome 8 at 21,665,846 bp
  • A to G, chromosome 8 at 27,393,327 bp
  • A to G, chromosome 8 at 70,591,538 bp
  • T to C, chromosome 8 at 88,345,191 bp
  • T to C, chromosome 9 at 15,292,076 bp
  • A to T, chromosome 9 at 109,494,683 bp
  • G to A, chromosome 10 at 53,349,101 bp
  • A to G, chromosome 10 at 72,654,952 bp
  • T to C, chromosome 11 at 69,883,601 bp
  • T to C, chromosome 11 at 70,238,426 bp
  • T to A, chromosome 11 at 70,771,440 bp
  • C to T, chromosome 11 at 101,468,999 bp
  • T to C, chromosome 11 at 119,463,366 bp
  • T to C, chromosome 12 at 34,052,324 bp
  • G to A, chromosome 13 at 93,186,313 bp
  • G to A, chromosome 14 at 34,267,608 bp
  • A to C, chromosome 14 at 76,111,985 bp
  • A to C, chromosome 14 at 89,898,297 bp
  • G to T, chromosome 15 at 10,574,936 bp
  • G to A, chromosome 16 at 5,167,044 bp
  • T to A, chromosome 16 at 65,540,250 bp
  • G to A, chromosome 17 at 15,041,455 bp
  • C to T, chromosome 17 at 20,029,586 bp
  • T to A, chromosome 17 at 38,417,244 bp
  • A to G, chromosome 18 at 38,198,807 bp
  • G to A, chromosome 19 at 23,694,322 bp
  • C to T, chromosome X at 48,159,521 bp
Phenotype
G1 male heterozygous mice are viable and fertile. The G1 mice were not characterized prior to archiving.
Strain Development
C57BL/6J mice were injected with ENU, resulting in G0 mice that were bred back to C57BL/6J. Sperm was archived from G1 male mice that produced viable G2 mice.
Suggested Control Mice
Wild-type littermates or C57BL/6J mice
MMRRC Genetic QC Summary
The MMRRC Centers have developed a genetic QC pipeline using MiniMUGA array genotyping to provide additional information on strain backgrounds for MMRRC congenic and inbred strains. For more information on when data may be available, or to request genotyping for a strain of interest, please contact mmrrc@missouri.edu. Older strains may not have this information.
Donor
Bruce Beutler, M.D., University of Texas Southwestern Medical Center.
Primary Reference
MUTAGENETIX record for R6333 (G1). B. Beutler and colleagues, Center for the Genetics of Host Defense, UT Southwestern, Dallas, TX.

Colony and Husbandry Information

Colony Surveillance Program and Current Health Reports

Mice recovered from a cryo-archive will have health surveillance performed on recipient females. Health reports will be provided prior to shipment. If you require additional health status information, please email mmrrc@missouri.edu.
Coat Color
Black
MMRRC Breeding System
Random intra-strain mating
Breeding Scheme(s)
When maintaining a live colony, heterozygous mice may be bred together, bred with wild-type siblings, or bred with C57BL/6J inbred mice.
Overall Breeding Performance
Undetermined; during development, mating was done with heterozygous males; viability, fertility and other breeding statistics for heterozygous females and homozygotes are unverified, but presumably would be similar to the C57BL/6J background strain
Average litter size
Undetermined
Recommended wean age
3 weeks

Order Request Information

Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.

Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.

The donor or their institution limits the distribution to non-profit institutions only.

Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.

Click button to Request this one strain. (Use the MMRRC Catalog Search to request more than one strain.)
MMRRC Item # Description Distribution Fee / Unit (US $)
*Shipping & Handling not included*
Units Notes
044487-MU-RESUS Litter recovered from cryo-archive $5,475.00 / Non-Profit Litter Recovered litter4; additional fees for any special requests.
Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

1 The distribution fee covers the expense of rederiving mice from a live mouse; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

2 An aliquot contains a sufficient number of embryos (in one or more vials or straws and based on the transfer success rate of the MMRRC facility) to transfer into one to three recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.

3 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.

4 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.