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Strain Name:
C57BL/6J-MtgxR6335Btlr/Mmmh
Stock Number:
044489-MU
Citation ID:
RRID:MMRRC_044489-MU
Other Names:
R6335 (G1)
Major Collection:

Strain Information

Iqgap1
Name: IQ motif containing GTPase activating protein 1
Synonyms: D7Ertd237e, D7Ertd257e
Type: Gene
Species: Mouse
Chromosome: 7
NCBI: 29875
HGNC: HGNC:6110
Homologene: 74514
Tead3
Name: TEA domain family member 3
Synonyms: ETFR-1, TEAD-3, DTEF-1, Tcf13r2, TEF-5
Type: Gene
Species: Mouse
Chromosome: 17
NCBI: 21678
Homologene: 81821
Mtor
Name: mechanistic target of rapamycin kinase
Synonyms: FKBP-rapamycin-associated protein FRAP, 2610315D21Rik, RAPT1, RAFT1, flat, Frap1, mechanistic target of rapamycin (serine/threonine kinase)
Type: Gene
Species: Mouse
Chromosome: 4
NCBI: 56717
HGNC: HGNC:3942
Homologene: 3637
Dcaf1
Name: DDB1 and CUL4 associated factor 1
Synonyms: B930007L02Rik, Vprbp
Type: Gene
Species: Mouse
Chromosome: 9
NCBI: 321006
Homologene: 8805
Prex2
Name: phosphatidylinositol-3,4,5-trisphosphate-dependent Rac exchange factor 2
Synonyms: 6230420N16Rik, C030045D06Rik, Depdc2
Type: Gene
Species: Mouse
Chromosome: 1
NCBI: 109294
Homologene: 23523
Ticrr
Name: TOPBP1-interacting checkpoint and replication regulator
Synonyms: 5730590G19Rik
Type: Gene
Species: Mouse
Chromosome: 7
NCBI: 77011
Homologene: 67120
Rexo1
Name: REX1, RNA exonuclease 1
Synonyms: 1700021P10Rik, 2610511M11Rik, Tceb3bp1, Rex1
Type: Gene
Species: Mouse
Chromosome: 10
NCBI: 66932
Homologene: 41391
Genetic Alterations
ENU-induced transitions at the following base pair locations (GRCm38):
  • A to C, chromosome 1 at 11,110,320 bp
  • A to T, chromosome 1 at 93,202,838 bp
  • T to A, chromosome 1 at 132,576,394 bp
  • A to G, chromosome 2 at 36,452,722 bp
  • T to A, chromosome 2 at 66,568,264 bp
  • T to A, chromosome 2 at 87,857,467 bp
  • T to C, chromosome 2 at 91,944,379 bp
  • T to C, chromosome 2 at 117,293,870 bp
  • C to A, chromosome 2 at 120,054,419 bp
  • T to C, chromosome 2 at 121,161,436 bp
  • T to C, chromosome 2 at 125,109,712 bp
  • A to G, chromosome 3 at 29,980,756 bp
  • T to C, chromosome 3 at 54,317,574 bp
  • A to G, chromosome 3 at 109,563,196 bp
  • G to T, chromosome 4 at 43,665,942 bp
  • A to G, chromosome 4 at 58,366,811 bp
  • A to C, chromosome 4 at 70,266,612 bp
  • A to G, chromosome 4 at 75,954,183 bp
  • G to T, chromosome 4 at 143,949,032 bp
  • A to G, chromosome 4 at 148,465,927 bp
  • A to T, chromosome 5 at 57,942,265 bp
  • T to C, chromosome 5 at 92,235,754 bp
  • G to T, chromosome 7 at 16,115,637 bp
  • A to G, chromosome 7 at 25,013,594 bp
  • A to C, chromosome 7 at 79,694,283 bp
  • A to G, chromosome 7 at 80,728,024 bp
  • A to G, chromosome 7 at 110,018,468 bp
  • T to C, chromosome 8 at 12,859,481 bp
  • A to G, chromosome 8 at 94,502,274 bp
  • C to A, chromosome 8 at 106,692,676 bp
  • A to G, chromosome 8 at 119,601,779 bp
  • T to G, chromosome 8 at 123,200,652 bp
  • T to A, chromosome 9 at 18,660,708 bp
  • C to T, chromosome 9 at 37,632,981 bp
  • A to G, chromosome 9 at 106,838,646 bp
  • T to C, chromosome 9 at 107,946,695 bp
  • C to T, chromosome 9 at 121,712,606 bp
  • A to T, chromosome 10 at 75,428,301 bp
  • A to T, chromosome 10 at 80,544,081 bp
  • G to T, chromosome 10 at 117,002,651 bp
  • C to T, chromosome 11 at 101,468,999 bp
  • C to T, chromosome 11 at 105,857,556 bp
  • A to G, chromosome 11 at 118,448,134 bp
  • A to T, chromosome 11 at 120,815,359 bp
  • T to C, chromosome 12 at 98,242,714 bp
  • G to T, chromosome 12 at 112,041,752 bp
  • T to A, chromosome 13 at 22,293,717 bp
  • C to A, chromosome 13 at 33,837,822 bp
  • A to G, chromosome 13 at 100,426,552 bp
  • G to T, chromosome 14 at 31,084,095 bp
  • T to C, chromosome 14 at 101,900,636 bp
  • A to G, chromosome 15 at 76,242,188 bp
  • A to G, chromosome 16 at 87,947,906 bp
  • A to T, chromosome 17 at 28,333,325 bp
  • T to C, chromosome 18 at 61,719,721 bp
  • T to A, chromosome 18 at 67,834,713 bp
  • G to T, chromosome 19 at 5,159,977 bp
  • T to C, chromosome 19 at 13,768,780 bp
  • T to A, chromosome 19 at 25,673,571 bp
  • C to T, chromosome X at 48,159,521 bp
Phenotype
G1 male heterozygous mice are viable and fertile. The G1 mice were not characterized prior to archiving.
Strain GQC Summary
Gene Specific Genotyping:

To request gene-specific and other genotyping services for a strain, please contact the distribution MMRRC Center for more information.

Background Genetic Quality:

The MMRRC has developed a Genetic Quality Control pipeline using the MiniMUGA array to provide additional information to identify and validate genetic backgrounds of MMRRC strains. For more information on whether genetic background data is available, please contact MMRRC_GeneticQC@med.unc.edu. Note: that MiniMUGA genetic background data is not available on all strains, but can be ordered if desired.

Suggested Control Mice
Littermates of all relevant genotypes.
Donor
Bruce Beutler, M.D., University of Texas Southwestern Medical Center.
Primary Reference
MUTAGENETIX record for R6335 (G1). B. Beutler and colleagues, Center for the Genetics of Host Defense, UT Southwestern, Dallas, TX.
Strain Development
C57BL/6J mice were injected with ENU, resulting in G0 mice that were bred back to C57BL/6J. Sperm was archived from G1 male mice that produced viable G2 mice.


Disclaimer: If MMRRC Strain Genetic Quality Control (GQC; based on MiniMUGA genotyping and analysis) has been completed for this strain, the information might differ from the genetic background information provided by the submitter. MiniMUGA genetic analysis is done on a strain’s tissue samples taken when archived by or ordered from the assigned MMRRC Center.

Colony and Husbandry Information

Colony Surveillance Program and Current Health Reports

Mice recovered from a cryo-archive will have health surveillance performed on recipient females. Health reports will be provided prior to shipment. If you require additional health status information, please email mmrrc@missouri.edu.
Coat Color
Black
MMRRC Breeding System
Random intra-strain mating
Breeding Scheme(s)
When maintaining a live colony, heterozygous mice may be bred together, bred with wild-type siblings, or bred with C57BL/6J inbred mice.
Overall Breeding Performance
Undetermined; during development, mating was done with heterozygous males; viability, fertility and other breeding statistics for heterozygous females and homozygotes are unverified, but presumably would be similar to the C57BL/6J background strain
Average litter size
Undetermined
Recommended wean age
3 weeks

Order Information

Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.

Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.

The donor or their institution limits the distribution to non-profit institutions only.

Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.

Click button to Request this one strain. (Use the MMRRC Catalog Search to request more than one strain.)
MMRRC Item # Description Distribution Fee / Unit (US $)
*Shipping & Handling not included*
Units Notes
044489-MU-RESUS Litter recovered from cryo-archive $5,475.00 / Non-Profit Litter Recovered litter4; additional fees for any special requests.
Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

1 The distribution fee covers the expense of rederiving mice from a live mouse; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

2 An aliquot contains a sufficient number of embryos (in one or more vials or straws and based on the transfer success rate of the MMRRC facility) to transfer into one to three recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.

3 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.

4 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.