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Strain Name:
C57BL/6J-MtgxR6362Btlr/Mmmh
Stock Number:
044512-MU
Citation ID:
RRID:MMRRC_044512-MU
Other Names:
R6362 (G1)
Major Collection:

Strain Information

Dspp
Name: dentin sialophosphoprotein
Synonyms: Dmp3, Dpp, Dsp
Type: Gene
Species: Mouse
Chromosome: 5
NCBI: 666279
HGNC: HGNC:3054
Colgalt2
Name: collagen beta(1-O)galactosyltransferase 2
Synonyms: Glt25d2
Type: Gene
Species: Mouse
Chromosome: 1
NCBI: 269132
Homologene: 22865
Met
Name: met proto-oncogene
Synonyms: HGF receptor, c-Met, Par4
Type: Gene
Species: Mouse
Chromosome: 6
NCBI: 17295
HGNC: HGNC:7029
Homologene: 206
Cacna1g
Name: calcium channel, voltage-dependent, T type, alpha 1G subunit
Synonyms: a1G, Cav3.1d
Type: Gene
Species: Mouse
Chromosome: 11
NCBI: 12291
HGNC: HGNC:1394
Homologene: 22544
Lefty1
Name: left right determination factor 1
Synonyms: lefty-1, Stra3, Lefty, Ebaf
Type: Gene
Species: Mouse
Chromosome: 1
NCBI: 13590
HGNC: HGNC:6552
Homologene: 49231
Mad1l1
Name: MAD1 mitotic arrest deficient 1-like 1
Synonyms: Mad1
Type: Gene
Species: Mouse
Chromosome: 5
NCBI: 17120
HGNC: HGNC:6762
Homologene: 74500
Srr
Name: serine racemase
Synonyms: M100034, Rgsc34
Type: Gene
Species: Mouse
Chromosome: 11
NCBI: 27364
Homologene: 22775
Genetic Alterations
ENU-induced transitions at the following base pair locations (GRCm38):
  • A to T, chromosome 1 at 88,275,050 bp
  • T to A, chromosome 1 at 90,810,563 bp
  • A to G, chromosome 1 at 133,680,596 bp
  • A to C, chromosome 1 at 152,471,798 bp
  • A to G, chromosome 1 at 180,937,160 bp
  • T to C, chromosome 2 at 52,212,692 bp
  • T to C, chromosome 2 at 84,859,784 bp
  • C to A, chromosome 2 at 85,991,941 bp
  • C to T, chromosome 2 at 87,033,289 bp
  • C to A, chromosome 2 at 121,025,779 bp
  • T to C, chromosome 2 at 122,598,196 bp
  • G to C, chromosome 2 at 127,431,918 bp
  • C to T, chromosome 2 at 156,048,250 bp
  • T to G, chromosome 2 at 173,528,174 bp
  • T to C, chromosome 3 at 37,419,489 bp
  • A to T, chromosome 3 at 94,434,988 bp
  • T to C, chromosome 4 at 24,629,195 bp
  • A to T, chromosome 4 at 43,416,416 bp
  • A to G, chromosome 4 at 96,071,695 bp
  • G to C, chromosome 4 at 130,066,190 bp
  • A to G, chromosome 4 at 143,852,865 bp
  • A to G, chromosome 4 at 148,254,308 bp
  • A to G, chromosome 5 at 104,176,034 bp
  • A to G, chromosome 5 at 115,886,676 bp
  • A to T, chromosome 5 at 140,315,055 bp
  • C to T, chromosome 6 at 17,558,733 bp
  • C to G, chromosome 6 at 92,458,615 bp
  • G to T, chromosome 7 at 3,675,459 bp
  • T to A, chromosome 7 at 4,129,324 bp
  • C to T, chromosome 7 at 4,629,836 bp
  • C to T, chromosome 7 at 27,351,695 bp
  • C to T, chromosome 7 at 42,903,297 bp
  • A to G, chromosome 7 at 46,376,881 bp
  • T to A, chromosome 7 at 46,647,443 bp
  • T to C, chromosome 7 at 80,923,131 bp
  • G to T, chromosome 7 at 85,751,174 bp
  • G to T, chromosome 7 at 109,934,265 bp
  • G to A, chromosome 7 at 127,136,723 bp
  • C to G, chromosome 8 at 25,811,807 bp
  • C to T, chromosome 8 at 85,119,650 bp
  • C to A, chromosome 8 at 105,331,641 bp
  • T to C, chromosome 8 at 119,941,316 bp
  • G to A, chromosome 9 at 44,986,291 bp
  • G to T, chromosome 9 at 66,471,908 bp
  • T to A, chromosome 9 at 110,895,446 bp
  • A to T, chromosome 10 at 39,921,609 bp
  • T to C, chromosome 10 at 54,074,795 bp
  • A to T, chromosome 10 at 105,369,970 bp
  • A to T, chromosome 10 at 111,273,068 bp
  • A to G, chromosome 10 at 115,478,525 bp
  • A to C, chromosome 10 at 129,436,943 bp
  • T to A, chromosome 11 at 50,838,475 bp
  • T to C, chromosome 11 at 70,554,675 bp
  • T to A, chromosome 11 at 74,910,202 bp
  • T to A, chromosome 11 at 76,411,127 bp
  • A to T, chromosome 11 at 94,439,707 bp
  • G to C, chromosome 11 at 103,620,652 bp
  • A to G, chromosome 11 at 115,583,734 bp
  • T to C, chromosome 11 at 120,064,690 bp
  • T to A, chromosome 12 at 112,920,122 bp
  • T to G, chromosome 13 at 24,918,550 bp
  • G to A, chromosome 14 at 14,116,949 bp
  • G to T, chromosome 14 at 41,967,770 bp
  • A to G, chromosome 14 at 86,772,130 bp
  • C to A, chromosome 15 at 10,984,805 bp
  • A to G, chromosome 15 at 79,588,823 bp
  • G to T, chromosome 15 at 79,643,350 bp
  • T to A, chromosome 15 at 100,940,115 bp
  • G to A, chromosome 15 at 103,308,510 bp
  • A to G, chromosome 16 at 31,989,884 bp
  • A to G, chromosome 16 at 44,218,577 bp
  • G to A, chromosome 16 at 64,786,094 bp
  • C to T, chromosome 16 at 96,002,307 bp
  • G to A, chromosome 17 at 6,991,694 bp
  • G to A, chromosome 17 at 33,911,364 bp
  • A to T, chromosome 17 at 34,694,388 bp
  • A to T, chromosome 17 at 38,082,729 bp
  • A to T, chromosome 18 at 20,035,463 bp
  • G to T, chromosome 18 at 24,754,255 bp
  • A to G, chromosome 18 at 37,670,905 bp
  • A to T, chromosome 19 at 13,063,345 bp
Phenotype
G1 male heterozygous mice are viable and fertile. The G1 mice were not characterized prior to archiving.
Strain GQC Summary
Gene Specific Genotyping:

To request gene-specific and other genotyping services for a strain, please contact the distribution MMRRC Center for more information.

Background Genetic Quality:

The MMRRC has developed a Genetic Quality Control pipeline using the MiniMUGA array to provide additional information to identify and validate genetic backgrounds of MMRRC strains. For more information on whether genetic background data is available, please contact MMRRC_GeneticQC@med.unc.edu. Note: that MiniMUGA genetic background data is not available on all strains, but can be ordered if desired.

Suggested Control Mice
Littermates of all relevant genotypes.
Donor
Bruce Beutler, M.D., University of Texas Southwestern Medical Center.
Primary Reference
MUTAGENETIX record for R6362 (G1). B. Beutler and colleagues, Center for the Genetics of Host Defense, UT Southwestern, Dallas, TX.
Strain Development
C57BL/6J mice were injected with ENU, resulting in G0 mice that were bred back to C57BL/6J. Sperm was archived from G1 male mice that produced viable G2 mice.


Disclaimer: If MMRRC Strain Genetic Quality Control (GQC; based on MiniMUGA genotyping and analysis) has been completed for this strain, the information might differ from the genetic background information provided by the submitter. MiniMUGA genetic analysis is done on a strain’s tissue samples taken when archived by or ordered from the assigned MMRRC Center.

Colony and Husbandry Information

Colony Surveillance Program and Current Health Reports

Mice recovered from a cryo-archive will have health surveillance performed on recipient females. Health reports will be provided prior to shipment. If you require additional health status information, please email mmrrc@missouri.edu.
Coat Color
Black
MMRRC Breeding System
Random intra-strain mating
Breeding Scheme(s)
When maintaining a live colony, heterozygous mice may be bred together, bred with wild-type siblings, or bred with C57BL/6J inbred mice.
Overall Breeding Performance
Undetermined; during development, mating was done with heterozygous males; viability, fertility and other breeding statistics for heterozygous females and homozygotes are unverified, but presumably would be similar to the C57BL/6J background strain
Average litter size
Undetermined
Recommended wean age
3 weeks

Order Information

Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.

Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.

The donor or their institution limits the distribution to non-profit institutions only.

Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.

Click button to Request this one strain. (Use the MMRRC Catalog Search to request more than one strain.)
MMRRC Item # Description Distribution Fee / Unit (US $)
*Shipping & Handling not included*
Units Notes
044512-MU-RESUS Litter recovered from cryo-archive $5,475.00 / Non-Profit Litter Recovered litter4; additional fees for any special requests.
Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

1 The distribution fee covers the expense of rederiving mice from a live mouse; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

2 An aliquot contains a sufficient number of embryos (in one or more vials or straws and based on the transfer success rate of the MMRRC facility) to transfer into one to three recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.

3 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.

4 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.