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Strain Name:
C57BL/6J-MtgxR6370Btlr/Mmmh
Stock Number:
044520-MU
Citation ID:
RRID:MMRRC_044520-MU
Other Names:
R6370 (G1)
Major Collection:

Strain Information

Chrnb4
Name: cholinergic receptor, nicotinic, beta polypeptide 4
Synonyms: Acrb-4, Acrb4
Type: Gene
Species: Mouse
Chromosome: 9
NCBI: 108015
VEGA: 9
HGNC: HGNC:1964
Homologene: 20196
Satb1
Name: special AT-rich sequence binding protein 1
Synonyms: 2610306G12Rik
Type: Gene
Species: Mouse
Chromosome: 17
NCBI: 20230
Homologene: 2232
Atic
Name: 5-aminoimidazole-4-carboxamide ribonucleotide formyltransferase/IMP cyclohydrolase
Synonyms: 2610509C24Rik
Type: Gene
Species: Mouse
Chromosome: 1
NCBI: 108147
HGNC: HGNC:794
Homologene: 2983
Lrrn1
Name: leucine rich repeat protein 1, neuronal
Synonyms: NLRR-1, 2810047E21Rik
Type: Gene
Species: Mouse
Chromosome: 6
NCBI: 16979
Homologene: 32036
Pik3cb
Name: phosphatidylinositol-4,5-bisphosphate 3-kinase catalytic subunit beta
Synonyms: 1110001J02Rik, p110beta
Type: Gene
Species: Mouse
Chromosome: 9
NCBI: 74769
HGNC: HGNC:8976
Homologene: 21250
Faah
Name: fatty acid amide hydrolase
Type: Gene
Species: Mouse
Chromosome: 4
NCBI: 14073
HGNC: HGNC:3553
Homologene: 68184
Ctcf
Name: CCCTC-binding factor
Type: Gene
Species: Mouse
Chromosome: 8
NCBI: 13018
Homologene: 4786
Genetic Alterations
ENU-induced transitions at the following base pair locations (GRCm38):
  • T to A, chromosome 1 at 71,578,660 bp
  • A to T, chromosome 1 at 139,822,327 bp
  • T to C, chromosome 1 at 167,634,437 bp
  • T to C, chromosome 1 at 184,795,492 bp
  • A to G, chromosome 2 at 5,976,010 bp
  • G to A, chromosome 2 at 25,397,417 bp
  • A to G, chromosome 2 at 30,827,012 bp
  • A to G, chromosome 2 at 44,756,322 bp
  • T to A, chromosome 2 at 88,499,368 bp
  • C to A, chromosome 2 at 130,443,384 bp
  • T to C, chromosome 2 at 166,065,905 bp
  • G to A, chromosome 3 at 19,691,486 bp
  • G to T, chromosome 4 at 106,380,521 bp
  • T to G, chromosome 4 at 113,614,110 bp
  • T to C, chromosome 4 at 116,003,056 bp
  • A to G, chromosome 5 at 148,340,673 bp
  • A to G, chromosome 6 at 107,569,224 bp
  • A to G, chromosome 6 at 120,865,622 bp
  • A to T, chromosome 6 at 124,820,428 bp
  • T to C, chromosome 7 at 10,098,216 bp
  • T to C, chromosome 7 at 26,763,358 bp
  • A to T, chromosome 7 at 29,932,410 bp
  • A to G, chromosome 7 at 34,357,481 bp
  • G to A, chromosome 7 at 42,820,586 bp
  • A to G, chromosome 7 at 44,395,683 bp
  • G to T, chromosome 7 at 45,076,740 bp
  • GCG to GCGACGGCGACG, chromosome 7 at 97,579,907 bp
  • A to T, chromosome 7 at 102,877,170 bp
  • T to C, chromosome 7 at 104,733,917 bp
  • A to G, chromosome 8 at 60,923,024 bp
  • C to T, chromosome 8 at 95,264,422 bp
  • T to C, chromosome 8 at 105,664,220 bp
  • T to A, chromosome 8 at 106,195,437 bp
  • G to T, chromosome 9 at 21,587,519 bp
  • T to C, chromosome 9 at 44,106,261 bp
  • A to T, chromosome 9 at 50,682,223 bp
  • A to G, chromosome 9 at 55,034,859 bp
  • A to G, chromosome 9 at 60,737,363 bp
  • A to T, chromosome 9 at 99,040,934 bp
  • C to T, chromosome 10 at 62,764,529 bp
  • C to T, chromosome 10 at 81,077,983 bp
  • C to T, chromosome 10 at 83,571,362 bp
  • T to C, chromosome 10 at 93,497,506 bp
  • A to G, chromosome 10 at 128,570,074 bp
  • T to C, chromosome 11 at 51,586,716 bp
  • T to A, chromosome 11 at 119,477,078 bp
  • A to G, chromosome 12 at 87,109,199 bp
  • A to T, chromosome 12 at 98,689,034 bp
  • A to T, chromosome 13 at 21,288,702 bp
  • G to A, chromosome 13 at 73,668,159 bp
  • C to A, chromosome 14 at 33,068,850 bp
  • T to C, chromosome 14 at 79,610,334 bp
  • A to G, chromosome 15 at 67,111,346 bp
  • T to A, chromosome 15 at 77,943,306 bp
  • T to C, chromosome 15 at 85,393,385 bp
  • T to C, chromosome 16 at 89,403,631 bp
  • A to T, chromosome 17 at 51,782,797 bp
Phenotype
G1 male heterozygous mice are viable and fertile. The G1 mice were not characterized prior to archiving.
Strain GQC Summary
Gene Specific Genotyping:

To request gene-specific and other genotyping services for a strain, please contact the distribution MMRRC Center for more information.

Background Genetic Quality:

The MMRRC has developed a Genetic Quality Control pipeline using the MiniMUGA array to provide additional information to identify and validate genetic backgrounds of MMRRC strains. For more information on whether genetic background data is available, please contact MMRRC_GeneticQC@med.unc.edu. Note: that MiniMUGA genetic background data is not available on all strains, but can be ordered if desired.

Suggested Control Mice
Littermates of all relevant genotypes.
Donor
Bruce Beutler, M.D., University of Texas Southwestern Medical Center.
Primary Reference
MUTAGENETIX record for R6370 (G1). B. Beutler and colleagues, Center for the Genetics of Host Defense, UT Southwestern, Dallas, TX.
Strain Development
C57BL/6J mice were injected with ENU, resulting in G0 mice that were bred back to C57BL/6J. Sperm was archived from G1 male mice that produced viable G2 mice.


Disclaimer: If MMRRC Strain Genetic Quality Control (GQC; based on MiniMUGA genotyping and analysis) has been completed for this strain, the information might differ from the genetic background information provided by the submitter. MiniMUGA genetic analysis is done on a strain’s tissue samples taken when archived by or ordered from the assigned MMRRC Center.

Colony and Husbandry Information

Colony Surveillance Program and Current Health Reports

Mice recovered from a cryo-archive will have health surveillance performed on recipient females. Health reports will be provided prior to shipment. If you require additional health status information, please email mmrrc@missouri.edu.
Coat Color
Black
MMRRC Breeding System
Random intra-strain mating
Breeding Scheme(s)
When maintaining a live colony, heterozygous mice may be bred together, bred with wild-type siblings, or bred with C57BL/6J inbred mice.
Overall Breeding Performance
Undetermined; during development, mating was done with heterozygous males; viability, fertility and other breeding statistics for heterozygous females and homozygotes are unverified, but presumably would be similar to the C57BL/6J background strain
Average litter size
Undetermined
Recommended wean age
3 weeks

Order Information

Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.

Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.

The donor or their institution limits the distribution to non-profit institutions only.

Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.

Click button to Request this one strain. (Use the MMRRC Catalog Search to request more than one strain.)
MMRRC Item # Description Distribution Fee / Unit (US $)
*Shipping & Handling not included*
Units Notes
044520-MU-RESUS Litter recovered from cryo-archive $5,475.00 / Non-Profit Litter Recovered litter4; additional fees for any special requests.
Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

1 The distribution fee covers the expense of rederiving mice from a live mouse; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

2 An aliquot contains a sufficient number of embryos (in one or more vials or straws and based on the transfer success rate of the MMRRC facility) to transfer into one to three recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.

3 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.

4 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.