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Strain Name:
C57BL/6J-MtgxR6382Btlr/Mmmh
Stock Number:
044531-MU
Citation ID:
RRID:MMRRC_044531-MU
Other Names:
R6382 (G1)
Major Collection:

Strain Information

Notch2
Name: notch 2
Synonyms: Motch B, N2
Type: Gene
Species: Mouse
Chromosome: 3
NCBI: 18129
HGNC: HGNC:7882
Homologene: 7865
Unc93b1
Name: unc-93 homolog B1, TLR signaling regulator
Type: Gene
Species: Mouse
Chromosome: 19
NCBI: 54445
Homologene: 41325
Hsp90aa1
Name: heat shock protein 90, alpha (cytosolic), class A member 1
Synonyms: hsp4, Hsp90, Hsp86-1, Hsp89, Hspca
Type: Gene
Species: Mouse
Chromosome: 12
NCBI: 15519
Homologene: 68464
Ufc1
Name: ubiquitin-fold modifier conjugating enzyme 1
Synonyms: ESTM29, 1110021H02Rik
Type: Gene
Species: Mouse
Chromosome: 1
NCBI: 66155
Homologene: 9500
Pard3
Name: par-3 family cell polarity regulator
Synonyms: ASIP, D8Ertd580e, PAR-3, Par3, Pard3a
Type: Gene
Species: Mouse
Chromosome: 8
NCBI: 93742
Homologene: 10489
Mdm2
Name: transformed mouse 3T3 cell double minute 2
Synonyms: Mdm-2, 1700007J15Rik
Type: Gene
Species: Mouse
Chromosome: 10
NCBI: 17246
HGNC: HGNC:6973
Homologene: 1793
Genetic Alterations
ENU-induced transitions at the following base pair locations (GRCm38):
  • A to G, chromosome 1 at 10,083,475 bp
  • C to T, chromosome 1 at 51,774,307 bp
  • A to G, chromosome 1 at 171,294,675 bp
  • A to G, chromosome 1 at 188,814,302 bp
  • A to T, chromosome 2 at 126,917,029 bp
  • G to C, chromosome 2 at 127,431,918 bp
  • A to T, chromosome 2 at 163,569,006 bp
  • A to G, chromosome 3 at 98,141,543 bp
  • A to G, chromosome 3 at 158,058,696 bp
  • T to C, chromosome 4 at 119,404,166 bp
  • T to C, chromosome 4 at 147,825,868 bp
  • A to T, chromosome 4 at 149,626,120 bp
  • C to T, chromosome 5 at 72,736,480 bp
  • C to A, chromosome 5 at 96,128,999 bp
  • T to A, chromosome 6 at 34,957,043 bp
  • T to A, chromosome 6 at 43,153,965 bp
  • T to A, chromosome 6 at 47,502,439 bp
  • A to G, chromosome 6 at 66,553,361 bp
  • A to G, chromosome 6 at 68,680,688 bp
  • C to T, chromosome 6 at 70,371,830 bp
  • A to G, chromosome 6 at 139,719,998 bp
  • A to G, chromosome 7 at 11,930,499 bp
  • C to A, chromosome 7 at 27,352,059 bp
  • A to G, chromosome 7 at 145,269,749 bp
  • T to A, chromosome 8 at 46,055,991 bp
  • T to A, chromosome 8 at 104,621,543 bp
  • T to A, chromosome 8 at 127,376,783 bp
  • T to A, chromosome 9 at 57,700,690 bp
  • C to A, chromosome 9 at 108,208,137 bp
  • G to A, chromosome 9 at 108,975,393 bp
  • T to C, chromosome 10 at 77,999,837 bp
  • G to A, chromosome 10 at 78,752,841 bp
  • C to A, chromosome 10 at 79,017,517 bp
  • A to G, chromosome 10 at 80,293,986 bp
  • A to G, chromosome 10 at 115,407,908 bp
  • A to G, chromosome 10 at 117,692,721 bp
  • A to T, chromosome 10 at 127,991,327 bp
  • C to T, chromosome 11 at 58,999,413 bp
  • A to T, chromosome 11 at 59,042,208 bp
  • T to C, chromosome 11 at 70,672,041 bp
  • T to C, chromosome 11 at 78,325,742 bp
  • T to C, chromosome 11 at 118,003,360 bp
  • A to T, chromosome 12 at 110,695,517 bp
  • T to C, chromosome 13 at 24,677,845 bp
  • T to A, chromosome 16 at 8,600,986 bp
  • T to C, chromosome 16 at 58,613,910 bp
  • T to C, chromosome 17 at 26,938,454 bp
  • T to C, chromosome 17 at 36,031,832 bp
  • C to G, chromosome 17 at 46,957,315 bp
  • A to G, chromosome 17 at 64,309,615 bp
  • G to A, chromosome 17 at 71,459,243 bp
  • A to G, chromosome 18 at 43,571,179 bp
  • C to A, chromosome 19 at 3,935,297 bp
  • T to A, chromosome 19 at 34,594,883 bp
  • A to G, chromosome 19 at 43,388,202 bp
  • TGAGACGAACTCTCCGAG to TGAG, chromosome X at 75,125,769 bp
Phenotype
G1 male heterozygous mice are viable and fertile. The G1 mice were not characterized prior to archiving.
Strain GQC Summary
Gene Specific Genotyping:

To request gene-specific and other genotyping services for a strain, please contact the distribution MMRRC Center for more information.

Background Genetic Quality:

The MMRRC has developed a Genetic Quality Control pipeline using the MiniMUGA array to provide additional information to identify and validate genetic backgrounds of MMRRC strains. For more information on whether genetic background data is available, please contact MMRRC_GeneticQC@med.unc.edu. Note: that MiniMUGA genetic background data is not available on all strains, but can be ordered if desired.

Suggested Control Mice
Littermates of all relevant genotypes.
Donor
Bruce Beutler, M.D., University of Texas Southwestern Medical Center.
Primary Reference
MUTAGENETIX record for R6382 (G1). B. Beutler and colleagues, Center for the Genetics of Host Defense, UT Southwestern, Dallas, TX.
Strain Development
C57BL/6J mice were injected with ENU, resulting in G0 mice that were bred back to C57BL/6J. Sperm was archived from G1 male mice that produced viable G2 mice.


Disclaimer: If MMRRC Strain Genetic Quality Control (GQC; based on MiniMUGA genotyping and analysis) has been completed for this strain, the information might differ from the genetic background information provided by the submitter. MiniMUGA genetic analysis is done on a strain’s tissue samples taken when archived by or ordered from the assigned MMRRC Center.

Colony and Husbandry Information

Colony Surveillance Program and Current Health Reports

Mice recovered from a cryo-archive will have health surveillance performed on recipient females. Health reports will be provided prior to shipment. If you require additional health status information, please email mmrrc@missouri.edu.
Coat Color
Black
MMRRC Breeding System
Random intra-strain mating
Breeding Scheme(s)
When maintaining a live colony, heterozygous mice may be bred together, bred with wild-type siblings, or bred with C57BL/6J inbred mice.
Overall Breeding Performance
Undetermined; during development, mating was done with heterozygous males; viability, fertility and other breeding statistics for heterozygous females and homozygotes are unverified, but presumably would be similar to the C57BL/6J background strain
Average litter size
Undetermined
Recommended wean age
3 weeks

Order Information

Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.

Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.

The donor or their institution limits the distribution to non-profit institutions only.

Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.

Click button to Request this one strain. (Use the MMRRC Catalog Search to request more than one strain.)
MMRRC Item # Description Distribution Fee / Unit (US $)
*Shipping & Handling not included*
Units Notes
044531-MU-RESUS Litter recovered from cryo-archive $5,475.00 / Non-Profit Litter Recovered litter4; additional fees for any special requests.
Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

1 The distribution fee covers the expense of rederiving mice from a live mouse; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

2 An aliquot contains a sufficient number of embryos (in one or more vials or straws and based on the transfer success rate of the MMRRC facility) to transfer into one to three recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.

3 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.

4 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.