Loading Mouse GIF
Loading...

Strain Name:
C57BL/6J-MtgxR6393Btlr/Mmmh
Stock Number:
044542-MU
Citation ID:
RRID:MMRRC_044542-MU
Other Names:
R6393 (G1)
Major Collection:

Strain Information

Ank2
Name: ankyrin 2, brain
Synonyms: ankyrin B, Ank-2, Ankyrin-2, Ankyrin-B, Gm4392
Type: Gene
Species: Mouse
Chromosome: 3
NCBI: 109676
HGNC: HGNC:493
Rcan2
Name: regulator of calcineurin 2
Synonyms: MCIP2, ZAKI-4, Csp2, Dscr1l1
Type: Gene
Species: Mouse
Chromosome: 17
NCBI: 53901
VEGA: 17
HGNC: HGNC:3041
Homologene: 130985
Tlr9
Name: toll-like receptor 9
Type: Gene
Species: Mouse
Chromosome: 9
NCBI: 81897
Homologene: 68126
Frem2
Name: Fras1 related extracellular matrix protein 2
Synonyms: 8430406N05Rik, 6030440P17Rik, my, ne, b2b1562Clo
Type: Gene
Species: Mouse
Chromosome: 3
NCBI: 242022
Homologene: 18454
Fancd2
Name: Fanconi anemia, complementation group D2
Synonyms: 2410150O07Rik
Type: Gene
Species: Mouse
Chromosome: 6
NCBI: 211651
HGNC: HGNC:3585
Homologene: 13212
Dock5
Name: dedicator of cytokinesis 5
Synonyms: lr2, 1110060D06Rik, rlc
Type: Gene
Species: Mouse
Chromosome: 14
NCBI: 68813
VEGA: 14
Homologene: 57016
Htr1b
Name: 5-hydroxytryptamine (serotonin) receptor 1B
Synonyms: 5-HT1B receptor, 5HT1B receptor, 5-HT1B receptor
Type: Gene
Species: Mouse
Chromosome: 9
NCBI: 15551
HGNC: HGNC:5287
Homologene: 669
Genetic Alterations
ENU-induced transitions at the following base pair locations (GRCm38):
  • A to G, chromosome 1 at 53,066,489 bp
  • T to A, chromosome 1 at 88,205,662 bp
  • A to T, chromosome 1 at 91,361,190 bp
  • A to G, chromosome 1 at 193,174,382 bp
  • T to C, chromosome 2 at 13,355,680 bp
  • A to G, chromosome 2 at 87,371,565 bp
  • A to G, chromosome 2 at 122,686,046 bp
  • G to C, chromosome 2 at 127,431,918 bp
  • A to G, chromosome 2 at 160,979,487 bp
  • G to T, chromosome 3 at 53,585,640 bp
  • A to C, chromosome 3 at 56,091,119 bp
  • A to G, chromosome 3 at 58,846,320 bp
  • C to A, chromosome 3 at 73,049,914 bp
  • G to A, chromosome 3 at 82,863,955 bp
  • A to G, chromosome 3 at 83,129,911 bp
  • G to A, chromosome 3 at 87,448,327 bp
  • A to G, chromosome 3 at 93,397,199 bp
  • A to G, chromosome 3 at 107,930,826 bp
  • G to A, chromosome 3 at 126,929,757 bp
  • A to G, chromosome 4 at 40,976,851 bp
  • T to C, chromosome 4 at 106,447,596 bp
  • T to G, chromosome 4 at 123,743,531 bp
  • T to A, chromosome 4 at 136,984,866 bp
  • A to G, chromosome 4 at 155,589,351 bp
  • C to A, chromosome 5 at 15,473,039 bp
  • A to G, chromosome 5 at 29,254,294 bp
  • T to C, chromosome 5 at 65,791,001 bp
  • C to T, chromosome 5 at 127,616,886 bp
  • A to G, chromosome 6 at 3,708,586 bp
  • GC to GCTCC, chromosome 6 at 4,756,452 bp
  • A to T, chromosome 6 at 90,269,322 bp
  • G to A, chromosome 6 at 90,325,081 bp
  • T to A, chromosome 6 at 113,578,413 bp
  • T to C, chromosome 6 at 121,336,842 bp
  • T to C, chromosome 6 at 122,315,380 bp
  • A to C, chromosome 6 at 123,316,153 bp
  • A to G, chromosome 6 at 136,328,598 bp
  • T to C, chromosome 7 at 4,957,622 bp
  • T to C, chromosome 7 at 5,030,066 bp
  • T to C, chromosome 7 at 10,900,901 bp
  • T to C, chromosome 7 at 65,969,014 bp
  • T to C, chromosome 7 at 105,809,915 bp
  • T to C, chromosome 7 at 139,019,171 bp
  • A to G, chromosome 8 at 18,948,700 bp
  • A to G, chromosome 9 at 14,785,509 bp
  • A to G, chromosome 9 at 15,274,583 bp
  • T to A, chromosome 9 at 18,647,399 bp
  • T to C, chromosome 9 at 79,655,485 bp
  • T to A, chromosome 9 at 81,631,757 bp
  • T to C, chromosome 9 at 106,224,937 bp
  • T to A, chromosome 10 at 24,873,476 bp
  • A to G, chromosome 11 at 97,463,672 bp
  • A to G, chromosome 12 at 4,278,181 bp
  • C to T, chromosome 13 at 46,752,455 bp
  • A to T, chromosome 13 at 97,994,019 bp
  • A to G, chromosome 14 at 57,638,313 bp
  • G to T, chromosome 14 at 59,773,755 bp
  • G to A, chromosome 14 at 67,822,602 bp
  • G to C, chromosome 14 at 70,309,813 bp
  • T to C, chromosome 15 at 10,393,808 bp
  • A to G, chromosome 15 at 11,255,635 bp
  • T to A, chromosome 16 at 45,096,465 bp
  • T to C, chromosome 17 at 26,921,842 bp
  • T to G, chromosome 17 at 33,137,776 bp
  • T to A, chromosome 17 at 43,953,479 bp
  • T to C, chromosome 17 at 46,782,622 bp
  • C to A, chromosome 17 at 56,721,331 bp
  • T to C, chromosome 18 at 12,479,756 bp
  • T to C, chromosome 18 at 33,205,225 bp
  • A to T, chromosome 18 at 84,013,220 bp
  • A to T, chromosome 19 at 11,870,727 bp
  • T to A, chromosome 19 at 29,437,298 bp
Phenotype
G1 male heterozygous mice are viable and fertile. The G1 mice were not characterized prior to archiving.
Strain GQC Summary
Gene Specific Genotyping:

To request gene-specific and other genotyping services for a strain, please contact the distribution MMRRC Center for more information.

Background Genetic Quality:

The MMRRC has developed a Genetic Quality Control pipeline using the MiniMUGA array to provide additional information to identify and validate genetic backgrounds of MMRRC strains. For more information on whether genetic background data is available, please contact MMRRC_GeneticQC@med.unc.edu. Note: that MiniMUGA genetic background data is not available on all strains, but can be ordered if desired.

Suggested Control Mice
Littermates of all relevant genotypes.
Donor
Bruce Beutler, M.D., University of Texas Southwestern Medical Center.
Primary Reference
MUTAGENETIX record for R6393 (G1). B. Beutler and colleagues, Center for the Genetics of Host Defense, UT Southwestern, Dallas, TX.
Strain Development
C57BL/6J mice were injected with ENU, resulting in G0 mice that were bred back to C57BL/6J. Sperm was archived from G1 male mice that produced viable G2 mice.


Disclaimer: If MMRRC Strain Genetic Quality Control (GQC; based on MiniMUGA genotyping and analysis) has been completed for this strain, the information might differ from the genetic background information provided by the submitter. MiniMUGA genetic analysis is done on a strain’s tissue samples taken when archived by or ordered from the assigned MMRRC Center.

Colony and Husbandry Information

Colony Surveillance Program and Current Health Reports

Mice recovered from a cryo-archive will have health surveillance performed on recipient females. Health reports will be provided prior to shipment. If you require additional health status information, please email mmrrc@missouri.edu.
Coat Color
Black
MMRRC Breeding System
Random intra-strain mating
Breeding Scheme(s)
When maintaining a live colony, heterozygous mice may be bred together, bred with wild-type siblings, or bred with C57BL/6J inbred mice.
Overall Breeding Performance
Undetermined; during development, mating was done with heterozygous males; viability, fertility and other breeding statistics for heterozygous females and homozygotes are unverified, but presumably would be similar to the C57BL/6J background strain
Average litter size
Undetermined
Recommended wean age
3 weeks

Order Information

Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.

Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.

The donor or their institution limits the distribution to non-profit institutions only.

Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.

Click button to Request this one strain. (Use the MMRRC Catalog Search to request more than one strain.)
MMRRC Item # Description Distribution Fee / Unit (US $)
*Shipping & Handling not included*
Units Notes
044542-MU-RESUS Litter recovered from cryo-archive $5,475.00 / Non-Profit Litter Recovered litter4; additional fees for any special requests.
Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

1 The distribution fee covers the expense of rederiving mice from a live mouse; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

2 An aliquot contains a sufficient number of embryos (in one or more vials or straws and based on the transfer success rate of the MMRRC facility) to transfer into one to three recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.

3 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.

4 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.