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Strain Name:
C57BL/6J-MtgxR6394Btlr/Mmmh
Stock Number:
044543-MU
Citation ID:
RRID:MMRRC_044543-MU
Other Names:
R6394 (G1)
Major Collection:

Strain Information

Setd5
Name: SET domain containing 5
Synonyms: 2900045N06Rik
Type: Gene
Species: Mouse
Chromosome: 6
NCBI: 72895
Homologene: 12485
Cacna1h
Name: calcium channel, voltage-dependent, T type, alpha 1H subunit
Synonyms: alpha13.2, Cav3.2, T-type Cav3.2
Type: Gene
Species: Mouse
Chromosome: 17
NCBI: 58226
HGNC: HGNC:1395
Homologene: 56913
Gcm2
Name: glial cells missing homolog 2
Synonyms: Gcm1-rs2
Type: Gene
Species: Mouse
Chromosome: 13
NCBI: 107889
VEGA: 13
HGNC: HGNC:4198
Homologene: 3490
Zmynd8
Name: zinc finger, MYND-type containing 8
Synonyms: 1110013E22Rik, 2010005I16Rik, ZMYND8, RACK7, 3632413B07Rik, Prkcbp1
Type: Gene
Species: Mouse
Chromosome: 2
NCBI: 228880
HGNC: HGNC:9397
Homologene: 32679
Rictor
Name: RPTOR independent companion of MTOR, complex 2
Synonyms: 6030405M08Rik, D530039E11Rik, 4921505C17Rik
Type: Gene
Species: Mouse
Chromosome: 15
NCBI: 78757
VEGA: 15
Homologene: 34317
Brd4
Name: bromodomain containing 4
Synonyms: MCAP, HUNK1, WI-11513
Type: Gene
Species: Mouse
Chromosome: 17
NCBI: 57261
Homologene: 136213
Prpf40a
Name: pre-mRNA processing factor 40A
Synonyms: FBP11, 2810012K09Rik, Fnbp3
Type: Gene
Species: Mouse
Chromosome: 2
NCBI: 56194
Homologene: 6377
Genetic Alterations
ENU-induced transitions at the following base pair locations (GRCm38):
  • A to T, chromosome 1 at 59,167,197 bp
  • T to A, chromosome 1 at 87,100,706 bp
  • A to G, chromosome 1 at 173,729,015 bp
  • A to G, chromosome 2 at 4,936,003 bp
  • A to T, chromosome 2 at 53,144,878 bp
  • T to C, chromosome 2 at 76,555,954 bp
  • C to T, chromosome 2 at 88,794,952 bp
  • A to G, chromosome 2 at 127,284,241 bp
  • A to G, chromosome 2 at 136,061,009 bp
  • C to T, chromosome 2 at 165,434,097 bp
  • C to A, chromosome 2 at 165,846,023 bp
  • C to T, chromosome 2 at 181,719,002 bp
  • A to G, chromosome 3 at 59,235,087 bp
  • T to C, chromosome 4 at 62,524,026 bp
  • C to A, chromosome 5 at 37,378,275 bp
  • T to TCCG, chromosome 6 at 4,756,451 bp
  • A to T, chromosome 6 at 40,921,792 bp
  • A to G, chromosome 6 at 42,307,590 bp
  • A to G, chromosome 6 at 42,313,238 bp
  • A to T, chromosome 6 at 73,155,418 bp
  • T to A, chromosome 6 at 90,417,623 bp
  • T to C, chromosome 6 at 91,787,201 bp
  • T to G, chromosome 6 at 113,115,544 bp
  • T to A, chromosome 6 at 132,755,076 bp
  • A to T, chromosome 6 at 141,487,511 bp
  • T to C, chromosome 6 at 144,041,313 bp
  • A to G, chromosome 7 at 10,040,326 bp
  • A to T, chromosome 7 at 13,260,963 bp
  • G to A, chromosome 7 at 19,201,163 bp
  • T to C, chromosome 7 at 24,422,307 bp
  • A to G, chromosome 7 at 56,215,981 bp
  • ATGGCG to ATGGCGACGGTGGCG, chromosome 7 at 97,579,904 bp
  • T to A, chromosome 7 at 104,635,702 bp
  • T to C, chromosome 7 at 104,636,027 bp
  • C to T, chromosome 7 at 108,255,763 bp
  • A to T, chromosome 8 at 4,736,176 bp
  • C to T, chromosome 8 at 60,941,291 bp
  • A to G, chromosome 8 at 95,557,192 bp
  • A to T, chromosome 9 at 7,168,331 bp
  • C to A, chromosome 9 at 7,386,316 bp
  • A to C, chromosome 9 at 13,245,490 bp
  • A to G, chromosome 9 at 15,074,101 bp
  • T to C, chromosome 9 at 40,000,705 bp
  • T to A, chromosome 9 at 62,735,266 bp
  • A to G, chromosome 9 at 66,395,059 bp
  • T to A, chromosome 9 at 75,445,470 bp
  • T to A, chromosome 9 at 98,507,820 bp
  • T to A, chromosome 9 at 119,661,320 bp
  • A to T, chromosome 10 at 9,899,231 bp
  • A to G, chromosome 10 at 80,496,013 bp
  • T to A, chromosome 10 at 107,642,943 bp
  • G to T, chromosome 10 at 129,911,920 bp
  • A to G, chromosome 11 at 43,225,637 bp
  • A to G, chromosome 11 at 53,436,697 bp
  • G to A, chromosome 11 at 78,231,065 bp
  • T to C, chromosome 11 at 85,339,672 bp
  • A to G, chromosome 11 at 102,914,410 bp
  • T to C, chromosome 11 at 106,148,883 bp
  • A to T, chromosome 12 at 5,094,830 bp
  • T to C, chromosome 12 at 5,137,720 bp
  • T to A, chromosome 12 at 70,255,213 bp
  • C to T, chromosome 12 at 75,990,495 bp
  • T to G, chromosome 13 at 41,109,897 bp
  • C to T, chromosome 13 at 46,752,455 bp
  • A to G, chromosome 14 at 44,285,677 bp
  • A to T, chromosome 14 at 52,202,585 bp
  • A to T, chromosome 14 at 76,107,412 bp
  • A to T, chromosome 14 at 78,522,589 bp
  • T to C, chromosome 15 at 6,769,309 bp
  • T to A, chromosome 15 at 39,143,737 bp
  • T to A, chromosome 15 at 84,699,724 bp
  • G to A, chromosome 17 at 25,387,481 bp
  • C to A, chromosome 17 at 32,224,147 bp
  • G to A, chromosome 17 at 33,199,513 bp
  • A to T, chromosome 17 at 35,620,166 bp
  • T to C, chromosome 18 at 36,686,786 bp
  • C to T, chromosome 19 at 20,383,849 bp
  • T to A, chromosome 19 at 58,761,598 bp
Phenotype
G1 male heterozygous mice are viable and fertile. The G1 mice were not characterized prior to archiving.
Strain GQC Summary
Gene Specific Genotyping:

To request gene-specific and other genotyping services for a strain, please contact the distribution MMRRC Center for more information.

Background Genetic Quality:

The MMRRC has developed a Genetic Quality Control pipeline using the MiniMUGA array to provide additional information to identify and validate genetic backgrounds of MMRRC strains. For more information on whether genetic background data is available, please contact MMRRC_GeneticQC@med.unc.edu. Note: that MiniMUGA genetic background data is not available on all strains, but can be ordered if desired.

Suggested Control Mice
Littermates of all relevant genotypes.
Donor
Bruce Beutler, M.D., University of Texas Southwestern Medical Center.
Primary Reference
MUTAGENETIX record for R6394 (G1). B. Beutler and colleagues, Center for the Genetics of Host Defense, UT Southwestern, Dallas, TX.
Strain Development
C57BL/6J mice were injected with ENU, resulting in G0 mice that were bred back to C57BL/6J. Sperm was archived from G1 male mice that produced viable G2 mice.


Disclaimer: If MMRRC Strain Genetic Quality Control (GQC; based on MiniMUGA genotyping and analysis) has been completed for this strain, the information might differ from the genetic background information provided by the submitter. MiniMUGA genetic analysis is done on a strain’s tissue samples taken when archived by or ordered from the assigned MMRRC Center.

Colony and Husbandry Information

Colony Surveillance Program and Current Health Reports

Mice recovered from a cryo-archive will have health surveillance performed on recipient females. Health reports will be provided prior to shipment. If you require additional health status information, please email mmrrc@missouri.edu.
Coat Color
Black
MMRRC Breeding System
Random intra-strain mating
Breeding Scheme(s)
When maintaining a live colony, heterozygous mice may be bred together, bred with wild-type siblings, or bred with C57BL/6J inbred mice.
Overall Breeding Performance
Undetermined; during development, mating was done with heterozygous males; viability, fertility and other breeding statistics for heterozygous females and homozygotes are unverified, but presumably would be similar to the C57BL/6J background strain
Average litter size
Undetermined
Recommended wean age
3 weeks

Order Information

Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.

Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.

The donor or their institution limits the distribution to non-profit institutions only.

Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.

Click button to Request this one strain. (Use the MMRRC Catalog Search to request more than one strain.)
MMRRC Item # Description Distribution Fee / Unit (US $)
*Shipping & Handling not included*
Units Notes
044543-MU-RESUS Litter recovered from cryo-archive $5,475.00 / Non-Profit Litter Recovered litter4; additional fees for any special requests.
Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

1 The distribution fee covers the expense of rederiving mice from a live mouse; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

2 An aliquot contains a sufficient number of embryos (in one or more vials or straws and based on the transfer success rate of the MMRRC facility) to transfer into one to three recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.

3 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.

4 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.