Strain Name:
C57BL/6J-MtgxR6401Btlr/Mmmh
Stock Number:
044548-MU
Citation ID:
RRID:MMRRC_044548-MU
Other Names:
R6401 (G1)
Major Collection:

Strain Information

Col2a1
Name: collagen, type II, alpha 1
Synonyms: M100856, Col2a, Col2, Rgsc413, Rgsc856, Col2a-1, Del1, Lpk, M100413
Type: Gene
Species: Mouse
Chromosome: 15
NCBI: 12824
HGNC: HGNC:2200
Homologene: 55607
Ext1
Name: exostosin glycosyltransferase 1
Type: Gene
Species: Mouse
Chromosome: 15
NCBI: 14042
HGNC: HGNC:3512
Homologene: 30957
Notch3
Name: notch 3
Synonyms: hpbk, N3
Type: Gene
Species: Mouse
Chromosome: 17
NCBI: 18131
HGNC: HGNC:7883
Homologene: 376
Rgs12
Name: regulator of G-protein signaling 12
Synonyms: 1200016K18Rik, 4632412M04Rik
Type: Gene
Species: Mouse
Chromosome: 5
NCBI: 71729
HGNC: HGNC:9994
Homologene: 2195
Zbtb32
Name: zinc finger and BTB domain containing 32
Synonyms: Tzfp, Rog, PLZP, 4930524C15Rik
Type: Gene
Species: Mouse
Chromosome: 7
NCBI: 58206
Homologene: 8661
Smg7
Name: SMG7 nonsense mediated mRNA decay factor
Synonyms: Smg-7 homolog, nonsense mediated mRNA decay factor (C. elegans), 9430023P16Rik
Type: Gene
Species: Mouse
Chromosome: 1
NCBI: 226517
Homologene: 32235
Prex2
Name: phosphatidylinositol-3,4,5-trisphosphate-dependent Rac exchange factor 2
Synonyms: 6230420N16Rik, Depdc2, C030045D06Rik
Type: Gene
Species: Mouse
Chromosome: 1
NCBI: 109294
Homologene: 23523
Mcmbp
Name: minichromosome maintenance complex binding protein
Synonyms: 1110007A13Rik
Type: Gene
Species: Mouse
Chromosome: 7
NCBI: 210711
Homologene: 11749
Mib1
Name: MIB E3 ubiquitin protein ligase 1
Synonyms: E430019M12Rik, mindbomb, skeletrophin, Mib, mind bomb-1
Type: Gene
Species: Mouse
Chromosome: 18
NCBI: 225164
Homologene: 10810
Xpo7
Name: exportin 7
Synonyms: Ranbp16, 4930506C02Rik
Type: Gene
Species: Mouse
Chromosome: 14
NCBI: 65246
VEGA: 14
Homologene: 22857
Tonsl
Name: tonsoku-like, DNA repair protein
Synonyms: Nfkbil2, 2810439M11Rik
Type: Gene
Species: Mouse
Chromosome: 15
NCBI: 72749
HGNC: HGNC:7801
Homologene: 22754
St7
Name: suppression of tumorigenicity 7
Synonyms: Fam4a2, 9430001H04Rik, HELG, SEN4, RAY1, TSG7
Type: Gene
Species: Mouse
Chromosome: 6
NCBI: 64213
Homologene: 10185
Cngb1
Name: cyclic nucleotide gated channel beta 1
Synonyms: BC016201, Cngb1, Cngb1b
Type: Gene
Species: Mouse
Chromosome: 8
NCBI: 333329
HGNC: HGNC:2151
Homologene: 136420
Ap3s1
Name: adaptor-related protein complex 3, sigma 1 subunit
Synonyms: [s]3A
Type: Gene
Species: Mouse
Chromosome: 18
NCBI: 11777
VEGA: 18
HGNC: HGNC:2013
Homologene: 4254
Ldb3
Name: LIM domain binding 3
Synonyms: cypher, ZASP
Type: Gene
Species: Mouse
Chromosome: 14
NCBI: 24131
VEGA: 14
Homologene: 134626
Nos3
Name: nitric oxide synthase 3, endothelial cell
Synonyms: 2310065A03Rik, ecNOS, Nos-3, eNOS
Type: Gene
Species: Mouse
Chromosome: 5
NCBI: 18127
HGNC: HGNC:7876
Homologene: 504
Fbn1
Name: fibrillin 1
Synonyms: Fib-1
Type: Gene
Species: Mouse
Chromosome: 2
NCBI: 14118
HGNC: HGNC:3603
Homologene: 30958
Ttn
Name: titin
Synonyms: connectin, shru, D330041I19Rik, 2310057K23Rik, mdm, D830007G01Rik, 2310074I15Rik, 1100001C23Rik, 2310036G12Rik, L56
Type: Gene
Species: Mouse
Chromosome: 2
NCBI: 22138
Homologene: 130650
Or1n1
Name: olfactory receptor family 1 subfamily N member 1
Synonyms: GA_x6K02T2NLDC-33554926-33553994, Olfr351, MOR127-2
Type: Gene
Species: Mouse
Chromosome: 2
NCBI: 258944
HGNC: HGNC:8221
Homologene: 10583
Vwa7
Name: von Willebrand factor A domain containing 7
Synonyms: G7c, D17H6S56E-3
Type: Gene
Species: Mouse
Chromosome: 17
NCBI: 27762
Homologene: 11895
Stk31
Name: serine threonine kinase 31
Synonyms: C330007K24Rik
Type: Gene
Species: Mouse
Chromosome: 6
NCBI: 77485
Homologene: 12677
Fsip2
Name: fibrous sheath-interacting protein 2
Synonyms: OTTMUSG00000013335
Type: Gene
Species: Mouse
Chromosome: 2
NCBI: 241516
Homologene: 110349
D930020B18Rik
Name: RIKEN cDNA D930020B18 gene
Type: Gene
Species: Mouse
Chromosome: 10
NCBI: 216393
Homologene: 87610
Or13f5
Name: olfactory receptor family 13 subfamily F member 5
Synonyms: GA_x6K02T2N78B-7168533-7167574, Olfr275, MOR262-2
Type: Gene
Species: Mouse
Chromosome: 4
NCBI: 258857
Homologene: 17418
Nrxn3
Name: neurexin III
Synonyms: 4933401A11Rik, neurexin III alpha, neurexin III beta, neurexin III alpha, neurexin III beta, 9330112C09Rik, D12Bwg0831e
Type: Gene
Species: Mouse
Chromosome: 12
NCBI: 18191
HGNC: HGNC:8010
Homologene: 83225
Spata22
Name: spermatogenesis associated 22
Synonyms: LOC380709
Type: Gene
Species: Mouse
Chromosome: 11
NCBI: 380709
Homologene: 13076
Rxfp2
Name: relaxin/insulin-like family peptide receptor 2
Synonyms: Gpr106, LGR8, Great
Type: Gene
Species: Mouse
Chromosome: 5
NCBI: 140498
Homologene: 15402
Vmn2r112
Name: vomeronasal 2, receptor 112
Synonyms: EG628185
Type: Gene
Species: Mouse
Chromosome: 17
NCBI: 628185
Homologene: 86604
Ccn5
Name: cellular communication network factor 5
Synonyms: Crgr4, rCop1, CCN5, Wisp2
Type: Gene
Species: Mouse
Chromosome: 2
NCBI: 22403
Homologene: 2882
Ano3
Name: anoctamin 3
Synonyms: Tmem16c, B230324K02Rik
Type: Gene
Species: Mouse
Chromosome: 2
NCBI: 228432
Homologene: 57147
Cyp3a16
Name: cytochrome P450, family 3, subfamily a, polypeptide 16
Type: Gene
Species: Mouse
Chromosome: 5
NCBI: 13114
HGNC: HGNC:2638
Homologene: 133568
Wscd2
Name: WSC domain containing 2
Synonyms: 4933413A10Rik, C530024P05Rik
Type: Gene
Species: Mouse
Chromosome: 5
NCBI: 320916
Homologene: 35372
Vcpkmt
Name: valosin containing protein lysine (K) methyltransferase
Synonyms: Gm71, LOC207965, Mettl21d
Type: Gene
Species: Mouse
Chromosome: 12
NCBI: 207965
VEGA: 12
Homologene: 56995
Or5b111
Name: olfactory receptor family 5 subfamily B member 111
Synonyms: Olfr1465, MOR202-28, GA_x6K02T2RE5P-3645346-3644423
Type: Gene
Species: Mouse
Chromosome: 19
NCBI: 258121
HGNC: HGNC:8324
Nt5c2
Name: 5'-nucleotidase, cytosolic II
Synonyms: cN-II, NT5B, PNT5
Type: Gene
Species: Mouse
Chromosome: 19
NCBI: 76952
HGNC: HGNC:8022
Homologene: 8158
Polm
Name: polymerase (DNA directed), mu
Synonyms: B230309I03Rik, Tdt-N
Type: Gene
Species: Mouse
Chromosome: 11
NCBI: 54125
HGNC: HGNC:9185
Homologene: 41170
Ifi209
Name: interferon activated gene 209
Synonyms: Ifix, Pyhin1, Pyhin-1
Type: Gene
Species: Mouse
Chromosome: 1
NCBI: 236312
HGNC: HGNC:5395
Homologene: 115929
Rfpl4b
Name: ret finger protein-like 4B
Synonyms: Gm3037, LOC215919, Gm62
Type: Gene
Species: Mouse
Chromosome: 10
NCBI: 215919
Homologene: 130122
Gm5431
Name: predicted gene 5431
Type: Gene
Species: Mouse
Chromosome: 11
NCBI: 432555
Tcp10b
Name: t-complex protein 10b
Synonyms: D17Leh66B, Tcp-10bt, D17Leh66ba, T66B-a, Tcp-10b
Type: Gene
Species: Mouse
Chromosome: 17
NCBI: 21462
Homologene: 83254
Ighv2-4
Name: immunoglobulin heavy variable V2-4
Type: Gene
Species: Mouse
Chromosome: 12
NCBI: 668389
Kif19b
Name: kinesin family member 19B
Synonyms: Gm4869
Type: Gene
Species: Mouse
Chromosome: 5
NCBI: 101055939
Homologene: 82478
Genetic Alterations
ENU-induced transitions at the following base pair locations (GRCm38):
  • T to A, chromosome 1 at 11,186,727 bp
  • A to G, chromosome 1 at 152,840,136 bp
  • T to C, chromosome 1 at 173,644,703 bp
  • A to T, chromosome 2 at 36,860,165 bp
  • A to T, chromosome 2 at 76,969,862 bp
  • A to G, chromosome 2 at 82,990,086 bp
  • T to A, chromosome 2 at 110,775,114 bp
  • C to A, chromosome 2 at 125,346,450 bp
  • T to C, chromosome 2 at 163,829,026 bp
  • A to C, chromosome 4 at 52,826,242 bp
  • A to G, chromosome 5 at 24,379,811 bp
  • T to C, chromosome 5 at 35,020,332 bp
  • A to T, chromosome 5 at 113,588,145 bp
  • A to T, chromosome 5 at 140,456,943 bp
  • T to C, chromosome 5 at 145,440,364 bp
  • A to G, chromosome 5 at 150,043,130 bp
  • T to A, chromosome 6 at 17,855,318 bp
  • A to G, chromosome 6 at 49,423,438 bp
  • A to C, chromosome 7 at 30,591,819 bp
  • G to T, chromosome 7 at 128,707,059 bp
  • A to G, chromosome 8 at 95,303,739 bp
  • T to C, chromosome 10 at 38,820,945 bp
  • A to G, chromosome 10 at 121,641,857 bp
  • A to T, chromosome 11 at 5,829,491 bp
  • T to A, chromosome 11 at 48,888,709 bp
  • T to C, chromosome 11 at 73,333,354 bp
  • C to A, chromosome 12 at 69,582,845 bp
  • C to A, chromosome 12 at 89,255,000 bp
  • G to T, chromosome 12 at 113,653,462 bp
  • A to T, chromosome 14 at 34,577,334 bp
  • A to G, chromosome 14 at 70,682,347 bp
  • T to C, chromosome 15 at 53,106,097 bp
  • T to C, chromosome 15 at 76,633,666 bp
  • T to C, chromosome 15 at 97,985,892 bp
  • C to A, chromosome 17 at 13,073,579 bp
  • T to A, chromosome 17 at 22,603,551 bp
  • T to A, chromosome 17 at 32,158,623 bp
  • G to A, chromosome 17 at 35,017,310 bp
  • T to A, chromosome 18 at 10,795,802 bp
  • A to C, chromosome 18 at 46,758,007 bp
  • T to C, chromosome 19 at 13,313,514 bp
  • A to G, chromosome 19 at 46,889,811 bp
Phenotype
G1 male heterozygous mice are viable and fertile. The G1 mice were not characterized prior to archiving.
Strain Development
C57BL/6J mice were injected with ENU, resulting in G0 mice that were bred back to C57BL/6J. Sperm was archived from G1 male mice that produced viable G2 mice.
Suggested Control Mice
Wild-type littermates or C57BL/6J mice
MMRRC Genetic QC Summary
The MMRRC Centers have developed a genetic QC pipeline using MiniMUGA array genotyping to provide additional information on strain backgrounds for MMRRC congenic and inbred strains. For more information on when data may be available, or to request genotyping for a strain of interest, please contact mmrrc@missouri.edu. Older strains may not have this information.
Donor
Bruce Beutler, M.D., University of Texas Southwestern Medical Center.
Primary Reference
MUTAGENETIX record for R6401 (G1). B. Beutler and colleagues, Center for the Genetics of Host Defense, UT Southwestern, Dallas, TX.

Colony and Husbandry Information

Colony Surveillance Program and Current Health Reports

Mice recovered from a cryo-archive will have health surveillance performed on recipient females. Health reports will be provided prior to shipment. If you require additional health status information, please email mmrrc@missouri.edu.
Coat Color
Black
MMRRC Breeding System
Random intra-strain mating
Breeding Scheme(s)
When maintaining a live colony, heterozygous mice may be bred together, bred with wild-type siblings, or bred with C57BL/6J inbred mice.
Overall Breeding Performance
Undetermined; during development, mating was done with heterozygous males; viability, fertility and other breeding statistics for heterozygous females and homozygotes are unverified, but presumably would be similar to the C57BL/6J background strain
Average litter size
Undetermined
Recommended wean age
3 weeks

Order Request Information

Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.

Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.

The donor or their institution limits the distribution to non-profit institutions only.

Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.

Click button to Request this one strain. (Use the MMRRC Catalog Search to request more than one strain.)
MMRRC Item # Description Distribution Fee / Unit (US $)
*Shipping & Handling not included*
Units Notes
044548-MU-RESUS Litter recovered from cryo-archive $5,475.00 / Non-Profit Litter Recovered litter4; additional fees for any special requests.
Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

1 The distribution fee covers the expense of rederiving mice from a live mouse; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

2 An aliquot contains a sufficient number of embryos (in one or more vials or straws and based on the transfer success rate of the MMRRC facility) to transfer into one to three recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.

3 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.

4 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.