Strain Name:
C57BL/6J-MtgxR6408Btlr/Mmmh
Stock Number:
044553-MU
Citation ID:
RRID:MMRRC_044553-MU
Other Names:
R6408 (G1)
Major Collection:

Strain Information

Crybg3
Name: beta-gamma crystallin domain containing 3
Synonyms: Gm9581
Type: Gene
Species: Mouse
Chromosome: 16
NCBI: 224273
Homologene: 28544
Diaph3
Name: diaphanous related formin 3
Synonyms: p134MDia2, mDia2, 4930417P13Rik, Drf3, Diap3
Type: Gene
Species: Mouse
Chromosome: 14
NCBI: 56419
VEGA: 14
Homologene: 84829
Bzw2
Name: basic leucine zipper and W2 domains 2
Synonyms: HSPC028, 1110001I24Rik
Type: Gene
Species: Mouse
Chromosome: 12
NCBI: 66912
VEGA: 12
Homologene: 8530
Polr3a
Name: polymerase (RNA) III (DNA directed) polypeptide A
Synonyms: RPC155, RPC1, 9330175N20Rik
Type: Gene
Species: Mouse
Chromosome: 14
NCBI: 218832
VEGA: 14
Homologene: 5124
Tbc1d8
Name: TBC1 domain family, member 8
Synonyms: BUB2-like protein 1, HBLP1, AD3, GRAM domain
Type: Gene
Species: Mouse
Chromosome: 1
NCBI: 54610
Homologene: 31421
Gab1
Name: growth factor receptor bound protein 2-associated protein 1
Type: Gene
Species: Mouse
Chromosome: 8
NCBI: 14388
HGNC: HGNC:4066
Homologene: 1542
Ralgapa1
Name: Ral GTPase activating protein, alpha subunit 1
Synonyms: 4930400K19Rik, 2310003F20Rik, Tulip1, Garnl1
Type: Gene
Species: Mouse
Chromosome: 12
NCBI: 56784
VEGA: 12
Homologene: 84805
Robo1
Name: roundabout guidance receptor 1
Synonyms: DUTT1
Type: Gene
Species: Mouse
Chromosome: 16
NCBI: 19876
Homologene: 2206
Slit2
Name: slit guidance ligand 2
Synonyms: Slil3, Drad-1, E130320P19Rik, E030015M03Rik, b2b1200.1Clo
Type: Gene
Species: Mouse
Chromosome: 5
NCBI: 20563
Homologene: 3516
Clca4b
Name: chloride channel accessory 4B
Synonyms: AI747448
Type: Gene
Species: Mouse
Chromosome: 3
NCBI: 99709
HGNC: HGNC:2018
Homologene: 40808
Azi2
Name: 5-azacytidine induced gene 2
Synonyms: AZ2
Type: Gene
Species: Mouse
Chromosome: 9
NCBI: 27215
Homologene: 8443
Ddx24
Name: DEAD box helicase 24
Synonyms: 2510027P10Rik, 1700055J08Rik, DEAD (Asp-Glu-Ala-Asp) box polypeptide 24
Type: Gene
Species: Mouse
Chromosome: 12
NCBI: 27225
VEGA: 12
Homologene: 10702
Rnf26rt
Name: ring finger protein 26, retrotransposed
Synonyms: Gm9008
Type: Gene
Species: Mouse
Chromosome: 6
NCBI: 668155
Cubn
Name: cubilin
Synonyms: D2Wsu88e, intrinsic factor-cobalamin receptor
Type: Gene
Species: Mouse
Chromosome: 2
NCBI: 65969
HGNC: HGNC:2548
Homologene: 37434
Spmip2
Name: sperm microtubule inner protein 2
Synonyms: Gm17359
Type: Gene
Species: Mouse
Chromosome: 3
NCBI: 100233207
Homologene: 105634
Ush2a
Name: usherin
Synonyms: MUSH2A, A930011D15Rik, LOC381317, LOC269160, A930037M10Rik, Ushrn, Ush2a
Type: Gene
Species: Mouse
Chromosome: 1
NCBI: 22283
Homologene: 66151
Srgap3
Name: SLIT-ROBO Rho GTPase activating protein 3
Synonyms: Arhgap14, D130026O08Rik
Type: Gene
Species: Mouse
Chromosome: 6
NCBI: 259302
Homologene: 56686
Vmn1r37
Name: vomeronasal 1 receptor 37
Synonyms: V1rc10
Type: Gene
Species: Mouse
Chromosome: 6
NCBI: 171183
Homologene: 110800
Dnah3
Name: dynein, axonemal, heavy chain 3
Synonyms: Dnahc3
Type: Gene
Species: Mouse
Chromosome: 7
NCBI: 381917
HGNC: HGNC:2949
Homologene: 19674
Zfp282
Name: zinc finger protein 282
Synonyms: HUB1
Type: Gene
Species: Mouse
Chromosome: 6
NCBI: 101095
Homologene: 2647
Psmb9
Name: proteasome (prosome, macropain) subunit, beta type 9 (large multifunctional peptidase 2)
Synonyms: Lmp-2, Lmp2
Type: Gene
Species: Mouse
Chromosome: 17
NCBI: 16912
HGNC: HGNC:9546
Homologene: 2094
Taok2
Name: TAO kinase 2
Synonyms: MAP3K17, TAO2, TAO1, PSK1, 1110033K02Rik
Type: Gene
Species: Mouse
Chromosome: 7
NCBI: 381921
Homologene: 74531
Acss1
Name: acyl-CoA synthetase short-chain family member 1
Synonyms: 1110032O15Rik, AceCS2, Acas2, Acas2l
Type: Gene
Species: Mouse
Chromosome: 2
NCBI: 68738
Homologene: 56037
4930407I10Rik
Name: RIKEN cDNA 4930407I10 gene
Synonyms: LOC328573
Type: Gene
Species: Mouse
Chromosome: 15
NCBI: 328573
VEGA: 15
Homologene: 130065
Shroom1
Name: shroom family member 1
Synonyms: 1300007L22Rik, Shrm1, Apx
Type: Gene
Species: Mouse
Chromosome: 11
NCBI: 71774
Homologene: 12411
Capn13
Name: calpain 13
Synonyms: LOC381122
Type: Gene
Species: Mouse
Chromosome: 17
NCBI: 381122
VEGA: 17
Homologene: 27026
Dlx4
Name: distal-less homeobox 4
Synonyms: DII D, Dlx-4, Dlx7
Type: Gene
Species: Mouse
Chromosome: 11
NCBI: 13394
HGNC: HGNC:2917
Homologene: 7292
Kif26b
Name: kinesin family member 26B
Synonyms: N-11 kinesin, D230039L06Rik
Type: Gene
Species: Mouse
Chromosome: 1
NCBI: 269152
Homologene: 18623
Drc1
Name: dynein regulatory complex subunit 1
Synonyms: LOC381738, Gm1060, b2b1654Clo, Ccdc164
Type: Gene
Species: Mouse
Chromosome: 5
NCBI: 381738
Homologene: 35977
Hs6st3
Name: heparan sulfate 6-O-sulfotransferase 3
Synonyms: 6OST3
Type: Gene
Species: Mouse
Chromosome: 14
NCBI: 50787
VEGA: 14
Homologene: 89201
Pcsk7
Name: proprotein convertase subtilisin/kexin type 7
Synonyms: SPC7
Type: Gene
Species: Mouse
Chromosome: 9
NCBI: 18554
VEGA: 9
HGNC: HGNC:8748
Homologene: 37955
Lrfn2
Name: leucine rich repeat and fibronectin type III domain containing 2
Synonyms: 5730420O05Rik, SALM1
Type: Gene
Species: Mouse
Chromosome: 17
NCBI: 70530
Homologene: 19182
Thoc2l
Name: THO complex subunit 2-like
Synonyms: Gm3179, BC005561
Type: Gene
Species: Mouse
Chromosome: 5
NCBI: 100042165
Gbp2b
Name: guanylate binding protein 2b
Synonyms: Mag-1, Mpa-1, Gbp-1, Mpa1, LIMIT, Gbp1
Type: Gene
Species: Mouse
Chromosome: 3
NCBI: 14468
Homologene: 137233
Amer2
Name: APC membrane recruitment 2
Synonyms: 2600011E07Rik, Amer2, Fam123a
Type: Gene
Species: Mouse
Chromosome: 14
NCBI: 72125
VEGA: 14
Homologene: 45141
D7Ertd443e
Name: DNA segment, Chr 7, ERATO Doi 443, expressed
Synonyms: 4933400E14Rik, Fats
Type: Gene
Species: Mouse
Chromosome: 7
NCBI: 71007
Homologene: 18998
Lrrc27
Name: leucine rich repeat containing 27
Synonyms: 1700071K18Rik, 2310044E02Rik
Type: Gene
Species: Mouse
Chromosome: 7
NCBI: 76612
Homologene: 19268
Ralb
Name: v-ral simian leukemia viral oncogene B
Synonyms: 5730472O18Rik
Type: Gene
Species: Mouse
Chromosome: 1
NCBI: 64143
HGNC: HGNC:9840
Homologene: 20632
Cyp4f16
Name: cytochrome P450, family 4, subfamily f, polypeptide 16
Synonyms: 2310021J05Rik
Type: Gene
Species: Mouse
Chromosome: 17
NCBI: 70101
Homologene: 135840
1700028K03Rik
Name: RIKEN cDNA 1700028K03 gene
Synonyms: SCRE, Spo16
Type: Gene
Species: Mouse
Chromosome: 5
NCBI: 76421
Homologene: 18810
Brdt
Name: bromodomain, testis-specific
Synonyms: 7420412D09Rik, Brd6, Fsrg3
Type: Gene
Species: Mouse
Chromosome: 5
NCBI: 114642
HGNC: HGNC:1105
Homologene: 21064
Pus7l
Name: pseudouridylate synthase 7-like
Synonyms: 3000003F02Rik
Type: Gene
Species: Mouse
Chromosome: 15
NCBI: 78895
Homologene: 12842
Dcaf15
Name: DDB1 and CUL4 associated factor 15
Type: Gene
Species: Mouse
Chromosome: 8
NCBI: 212123
Homologene: 18182
Gjb5
Name: gap junction protein, beta 5
Synonyms: Cnx31.1, connexin 31.1, Gjb-5, Cx31.1
Type: Gene
Species: Mouse
Chromosome: 4
NCBI: 14622
HGNC: HGNC:4287
Homologene: 3858
Mettl6
Name: methyltransferase 6, methylcytidine
Synonyms: 1600013P15Rik
Type: Gene
Species: Mouse
Chromosome: 14
NCBI: 67011
VEGA: 14
Homologene: 12069
Raet1e
Name: retinoic acid early transcript 1E
Synonyms: Rae-1 epsilon
Type: Gene
Species: Mouse
Chromosome: 10
NCBI: 379043
Homologene: 134115
Genetic Alterations
ENU-induced transitions at the following base pair locations (GRCm38):
  • T to A, chromosome 1 at 39,402,899 bp
  • A to C, chromosome 1 at 119,478,109 bp
  • T to C, chromosome 1 at 178,917,568 bp
  • G to T, chromosome 1 at 188,267,032 bp
  • C to T, chromosome 2 at 13,294,203 bp
  • A to G, chromosome 2 at 150,628,492 bp
  • A to G, chromosome 3 at 79,449,399 bp
  • T to C, chromosome 3 at 142,618,138 bp
  • A to G, chromosome 3 at 144,919,275 bp
  • A to T, chromosome 4 at 127,356,147 bp
  • A to G, chromosome 5 at 30,356,288 bp
  • A to G, chromosome 5 at 47,984,986 bp
  • A to T, chromosome 5 at 74,685,646 bp
  • A to T, chromosome 5 at 76,343,971 bp
  • A to G, chromosome 5 at 104,518,777 bp
  • A to G, chromosome 5 at 107,385,492 bp
  • T to C, chromosome 5 at 107,543,992 bp
  • G to A, chromosome 6 at 47,880,385 bp
  • A to G, chromosome 6 at 66,731,579 bp
  • A to G, chromosome 6 at 76,496,458 bp
  • T to C, chromosome 6 at 112,723,006 bp
  • T to C, chromosome 7 at 119,922,968 bp
  • A to G, chromosome 7 at 126,870,992 bp
  • G to T, chromosome 7 at 134,349,711 bp
  • A to G, chromosome 7 at 139,218,268 bp
  • C to A, chromosome 8 at 80,788,597 bp
  • T to C, chromosome 8 at 84,104,726 bp
  • T to C, chromosome 9 at 45,909,696 bp
  • A to T, chromosome 9 at 118,061,482 bp
  • A to G, chromosome 10 at 22,180,746 bp
  • A to C, chromosome 11 at 53,463,387 bp
  • A to G, chromosome 11 at 95,145,252 bp
  • C to T, chromosome 12 at 36,107,525 bp
  • C to A, chromosome 12 at 55,683,910 bp
  • A to C, chromosome 12 at 103,425,560 bp
  • A to T, chromosome 14 at 24,486,871 bp
  • T to C, chromosome 14 at 31,479,726 bp
  • C to A, chromosome 14 at 52,770,167 bp
  • T to C, chromosome 14 at 60,380,225 bp
  • T to C, chromosome 14 at 86,828,994 bp
  • C to A, chromosome 14 at 119,138,634 bp
  • T to C, chromosome 15 at 82,065,106 bp
  • A to G, chromosome 15 at 94,531,575 bp
  • T to A, chromosome 16 at 59,495,690 bp
  • T to C, chromosome 16 at 72,972,046 bp
  • T to A, chromosome 17 at 32,551,199 bp
  • G to T, chromosome 17 at 34,185,733 bp
  • A to G, chromosome 17 at 49,070,626 bp
  • A to T, chromosome 17 at 73,365,959 bp
Phenotype
G1 male heterozygous mice are viable and fertile. The G1 mice were not characterized prior to archiving.
Strain Development
C57BL/6J mice were injected with ENU, resulting in G0 mice that were bred back to C57BL/6J. Sperm was archived from G1 male mice that produced viable G2 mice.
Suggested Control Mice
Wild-type littermates or C57BL/6J mice
MMRRC Genetic QC Summary
The MMRRC Centers have developed a genetic QC pipeline using MiniMUGA array genotyping to provide additional information on strain backgrounds for MMRRC congenic and inbred strains. For more information on when data may be available, or to request genotyping for a strain of interest, please contact mmrrc@missouri.edu. Older strains may not have this information.
Donor
Bruce Beutler, M.D., University of Texas Southwestern Medical Center.
Primary Reference
MUTAGENETIX record for R6408 (G1). B. Beutler and colleagues, Center for the Genetics of Host Defense, UT Southwestern, Dallas, TX.

Colony and Husbandry Information

Colony Surveillance Program and Current Health Reports

Mice recovered from a cryo-archive will have health surveillance performed on recipient females. Health reports will be provided prior to shipment. If you require additional health status information, please email mmrrc@missouri.edu.
Coat Color
Black
MMRRC Breeding System
Random intra-strain mating
Breeding Scheme(s)
When maintaining a live colony, heterozygous mice may be bred together, bred with wild-type siblings, or bred with C57BL/6J inbred mice.
Overall Breeding Performance
Undetermined; during development, mating was done with heterozygous males; viability, fertility and other breeding statistics for heterozygous females and homozygotes are unverified, but presumably would be similar to the C57BL/6J background strain
Average litter size
Undetermined
Recommended wean age
3 weeks

Order Request Information

Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.

Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.

The donor or their institution limits the distribution to non-profit institutions only.

Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.

Click button to Request this one strain. (Use the MMRRC Catalog Search to request more than one strain.)
MMRRC Item # Description Distribution Fee / Unit (US $)
*Shipping & Handling not included*
Units Notes
044553-MU-RESUS Litter recovered from cryo-archive $5,475.00 / Non-Profit Litter Recovered litter4; additional fees for any special requests.
Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

1 The distribution fee covers the expense of rederiving mice from a live mouse; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

2 An aliquot contains a sufficient number of embryos (in one or more vials or straws and based on the transfer success rate of the MMRRC facility) to transfer into one to three recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.

3 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.

4 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.