Strain Name:
C57BL/6J-MtgxR6422Btlr/Mmmh
Stock Number:
044563-MU
Citation ID:
RRID:MMRRC_044563-MU
Other Names:
R6422 (G1)
Major Collection:

Strain Information

Il4i1
Name: interleukin 4 induced 1
Synonyms: H-46, Fig1, Fig1-ps, H4, H-4, H46
Type: Gene
Species: Mouse
Chromosome: 7
NCBI: 14204
Homologene: 22567
Tyw5
Name: tRNA-yW synthesizing protein 5
Synonyms: 1110034B05Rik
Type: Gene
Species: Mouse
Chromosome: 1
NCBI: 68736
Homologene: 72085
Gcn1
Name: GCN1 activator of EIF2AK4
Synonyms: G431004K08Rik, GCN1L, Gcn1l1
Type: Gene
Species: Mouse
Chromosome: 5
NCBI: 231659
HGNC: HGNC:4199
Homologene: 5887
Cabin1
Name: calcineurin binding protein 1
Synonyms: A330070M20Rik, Cain, Ppp3in
Type: Gene
Species: Mouse
Chromosome: 10
NCBI: 104248
VEGA: 10
Homologene: 49307
Dip2b
Name: disco interacting protein 2 homolog B
Type: Gene
Species: Mouse
Chromosome: 15
NCBI: 239667
Homologene: 72227
Btbd9
Name: BTB domain containing 9
Synonyms: 1700023F20Rik
Type: Gene
Species: Mouse
Chromosome: 17
NCBI: 224671
VEGA: 17
Homologene: 14995
Kif26a
Name: kinesin family member 26A
Synonyms: N-11 kinesin
Type: Gene
Species: Mouse
Chromosome: 12
NCBI: 668303
Homologene: 18970
Adam11
Name: a disintegrin and metallopeptidase domain 11
Synonyms: Mdc
Type: Gene
Species: Mouse
Chromosome: 11
NCBI: 11488
HGNC: HGNC:189
Homologene: 7614
Dlst
Name: dihydrolipoamide S-succinyltransferase
Synonyms: 4632413C10Rik, 4930529O08Rik, 1600017E01Rik
Type: Gene
Species: Mouse
Chromosome: 12
NCBI: 78920
VEGA: 12
HGNC: HGNC:2911
Homologene: 1456
Rsrc1
Name: arginine/serine-rich coiled-coil 1
Synonyms: 1200013F24Rik, SRrp53
Type: Gene
Species: Mouse
Chromosome: 3
NCBI: 66880
Homologene: 41147
Paxbp1
Name: PAX3 and PAX7 binding protein 1
Synonyms: Pax3/7bp, 1810007M14Rik, Gcfc1
Type: Gene
Species: Mouse
Chromosome: 16
NCBI: 67367
Homologene: 9604
Tbc1d32
Name: TBC1 domain family, member 32
Synonyms: C6orf170, b2b2284Clo, D630037F22Rik, Bromi
Type: Gene
Species: Mouse
Chromosome: 10
NCBI: 544696
VEGA: 10
Homologene: 51889
Snapc4
Name: small nuclear RNA activating complex, polypeptide 4
Synonyms: 5730436L13Rik
Type: Gene
Species: Mouse
Chromosome: 2
NCBI: 227644
Homologene: 2321
Cep162
Name: centrosomal protein 162
Synonyms: 4922501C03Rik
Type: Gene
Species: Mouse
Chromosome: 9
NCBI: 382090
Homologene: 8930
Cep85l
Name: centrosomal protein 85-like
Synonyms: Gm9766
Type: Gene
Species: Mouse
Chromosome: 10
NCBI: 100038725
VEGA: 10
Homologene: 52598
Plxnb1
Name: plexin B1
Synonyms: 2900002G15Rik
Type: Gene
Species: Mouse
Chromosome: 9
NCBI: 235611
HGNC: HGNC:9103
Homologene: 130508
Notch4
Name: notch 4
Synonyms: Int-3, N4, Int3
Type: Gene
Species: Mouse
Chromosome: 17
NCBI: 18132
HGNC: HGNC:7884
Homologene: 3351
Col20a1
Name: collagen, type XX, alpha 1
Synonyms: 1700051I12Rik
Type: Gene
Species: Mouse
Chromosome: 2
NCBI: 73368
Homologene: 75138
Prr5
Name: proline rich 5 (renal)
Synonyms: Protor-1, C030017C09Rik
Type: Gene
Species: Mouse
Chromosome: 15
NCBI: 109270
Homologene: 17132
Slc5a4b
Name: solute carrier family 5 (neutral amino acid transporters, system A), member 4b
Synonyms: SGLT3b, 2010104G07Rik, SAAT1, pSGLT2
Type: Gene
Species: Mouse
Chromosome: 10
NCBI: 64454
Homologene: 56968
Dsc1
Name: desmocollin 1
Synonyms: Dsc1b, Dsc1a
Type: Gene
Species: Mouse
Chromosome: 18
NCBI: 13505
VEGA: 18
HGNC: HGNC:3035
Homologene: 22761
Tmprss11d
Name: transmembrane protease, serine 11d
Synonyms: AsP
Type: Gene
Species: Mouse
Chromosome: 5
NCBI: 231382
Homologene: 74519
Ptpn7
Name: protein tyrosine phosphatase, non-receptor type 7
Synonyms: BPTP-4, C920001D21Rik, LC-PTP
Type: Gene
Species: Mouse
Chromosome: 1
NCBI: 320139
HGNC: HGNC:9659
Homologene: 15411
Or51v14
Name: olfactory receptor family 51 subfamily V member 14
Synonyms: GA_x6K02T2PBJ9-6335095-6334154, Olfr620, MOR4-1
Type: Gene
Species: Mouse
Chromosome: 7
NCBI: 258808
Homologene: 103777
Ndst1
Name: N-deacetylase/N-sulfotransferase (heparan glucosaminyl) 1
Synonyms: glucosaminyl N-deacetylase/N-sulfotransferase 1, Ndst-1, Hsst, 1200015G06Rik, b2b2230Clo
Type: Gene
Species: Mouse
Chromosome: 18
NCBI: 15531
VEGA: 18
HGNC: HGNC:7680
Homologene: 20386
Tpp1
Name: tripeptidyl peptidase I
Synonyms: Cln2
Type: Gene
Species: Mouse
Chromosome: 7
NCBI: 12751
HGNC: HGNC:2073
Homologene: 335
Castor2
Name: cytosolic arginine sensor for mTORC1 subunit 2
Synonyms: Gats, Gatsl2
Type: Gene
Species: Mouse
Chromosome: 5
NCBI: 80909
Homologene: 84866
Lat2
Name: linker for activation of T cells family, member 2
Synonyms: Wbscr5, Wbscr15
Type: Gene
Species: Mouse
Chromosome: 5
NCBI: 56743
Homologene: 11297
Gm8159
Name: predicted gene 8159
Type: Gene
Species: Mouse
Chromosome: 14
NCBI: 666549
Homologene: 115686
Sall2
Name: spalt like transcription factor 2
Synonyms: Msal-2
Type: Gene
Species: Mouse
Chromosome: 14
NCBI: 50524
Homologene: 9269
A430005L14Rik
Name: RIKEN cDNA A430005L14 gene
Type: Gene
Species: Mouse
Chromosome: 4
NCBI: 97159
Homologene: 18433
Genetic Alterations
ENU-induced transitions at the following base pair locations (GRCm38):
  • G to A, chromosome 1 at 57,401,411 bp
  • G to T, chromosome 1 at 135,134,502 bp
  • A to G, chromosome 2 at 26,368,303 bp
  • G to A, chromosome 2 at 175,170,381 bp
  • C to A, chromosome 2 at 181,014,819 bp
  • C to T, chromosome 3 at 66,994,649 bp
  • T to A, chromosome 4 at 153,960,924 bp
  • T to A, chromosome 5 at 86,309,425 bp
  • T to C, chromosome 5 at 115,609,544 bp
  • T to A, chromosome 5 at 134,135,710 bp
  • T to C, chromosome 5 at 134,603,161 bp
  • G to T, chromosome 7 at 44,840,136 bp
  • T to G, chromosome 7 at 103,612,014 bp
  • G to A, chromosome 7 at 105,746,956 bp
  • G to T, chromosome 9 at 87,232,016 bp
  • A to G, chromosome 9 at 109,108,924 bp
  • A to T, chromosome 10 at 53,291,780 bp
  • A to T, chromosome 10 at 56,028,061 bp
  • A to G, chromosome 10 at 75,656,792 bp
  • C to A, chromosome 10 at 76,103,862 bp
  • A to G, chromosome 11 at 102,774,283 bp
  • T to A, chromosome 12 at 85,130,885 bp
  • T to G, chromosome 12 at 112,168,875 bp
  • T to C, chromosome 14 at 4,635,211 bp
  • A to C, chromosome 14 at 52,312,724 bp
  • C to T, chromosome 15 at 84,693,804 bp
  • T to A, chromosome 15 at 100,199,011 bp
  • A to T, chromosome 16 at 91,023,444 bp
  • G to A, chromosome 17 at 30,530,256 bp
  • T to C, chromosome 17 at 34,584,559 bp
  • A to C, chromosome 18 at 20,095,033 bp
  • G to T, chromosome 18 at 60,702,953 bp
Phenotype
G1 male heterozygous mice are viable and fertile. The G1 mice were not characterized prior to archiving.
Strain Development
C57BL/6J mice were injected with ENU, resulting in G0 mice that were bred back to C57BL/6J. Sperm was archived from G1 male mice that produced viable G2 mice.
Suggested Control Mice
Wild-type littermates or C57BL/6J mice
MMRRC Genetic QC Summary
The MMRRC Centers have developed a genetic QC pipeline using MiniMUGA array genotyping to provide additional information on strain backgrounds for MMRRC congenic and inbred strains. For more information on when data may be available, or to request genotyping for a strain of interest, please contact mmrrc@missouri.edu. Older strains may not have this information.
Donor
Bruce Beutler, M.D., University of Texas Southwestern Medical Center.
Primary Reference
MUTAGENETIX record for R6422 (G1). B. Beutler and colleagues, Center for the Genetics of Host Defense, UT Southwestern, Dallas, TX.

Colony and Husbandry Information

Colony Surveillance Program and Current Health Reports

Mice recovered from a cryo-archive will have health surveillance performed on recipient females. Health reports will be provided prior to shipment. If you require additional health status information, please email mmrrc@missouri.edu.
Coat Color
Black
MMRRC Breeding System
Random intra-strain mating
Breeding Scheme(s)
When maintaining a live colony, heterozygous mice may be bred together, bred with wild-type siblings, or bred with C57BL/6J inbred mice.
Overall Breeding Performance
Undetermined; during development, mating was done with heterozygous males; viability, fertility and other breeding statistics for heterozygous females and homozygotes are unverified, but presumably would be similar to the C57BL/6J background strain
Average litter size
Undetermined
Recommended wean age
3 weeks

Order Request Information

Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.

Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.

The donor or their institution limits the distribution to non-profit institutions only.

Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.

Click button to Request this one strain. (Use the MMRRC Catalog Search to request more than one strain.)
MMRRC Item # Description Distribution Fee / Unit (US $)
*Shipping & Handling not included*
Units Notes
044563-MU-RESUS Litter recovered from cryo-archive $5,475.00 / Non-Profit Litter Recovered litter4; additional fees for any special requests.
Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

1 The distribution fee covers the expense of rederiving mice from a live mouse; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

2 An aliquot contains a sufficient number of embryos (in one or more vials or straws and based on the transfer success rate of the MMRRC facility) to transfer into one to three recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.

3 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.

4 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.