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Strain Detail Sheet

Strain Name:
C57BL/6J-MtgxR6431Btlr/Mmmh
Stock Number:
044569-MU
Citation ID:
RRID:MMRRC_044569-MU
Other Names:
R6431 (G1)
Major Collection:
Beutler Mutagenetix

Strain Information

Gene Details

Fn1
Name: fibronectin 1
Synonyms: Fn-1, Fn
Type: Gene
Species: Mouse
Chromosome: 1
NCBI: 14268
HGNC: HGNC:3778
Homologene: 1533

Additional Resources
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IMSR
Gene Ontology
BioGPS
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Mouse Mine
KOMP Phenotyping
Monarch
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ClinGen
Slc32a1
Name: solute carrier family 32 (GABA vesicular transporter), member 1
Synonyms: R75019, VGAT, Viaat
Type: Gene
Species: Mouse
Chromosome: 2
NCBI: 22348
Homologene: 56451

Additional Resources
IMPC
Google
Entrez
PubMed
IMSR
Gene Ontology
BioGPS
Gene Cloud
IGTC
Mouse Phenome DB
Mouse Mine
KOMP Phenotyping
Monarch
GeneCards
ClinGen
Bpifb3
Name: BPI fold containing family B, member 3
Synonyms: Rya3
Type: Gene
Species: Mouse
Chromosome: 2
NCBI: 378700
Homologene: 18807

Additional Resources
IMPC
Google
Entrez
PubMed
IMSR
Gene Ontology
BioGPS
Gene Cloud
IGTC
Mouse Phenome DB
Mouse Mine
KOMP Phenotyping
Monarch
GeneCards
ClinGen
Pum1
Name: pumilio RNA-binding family member 1
Synonyms: Pumm
Type: Gene
Species: Mouse
Chromosome: 4
NCBI: 80912
Homologene: 22830

Additional Resources
IMPC
Google
Entrez
PubMed
IMSR
Gene Ontology
BioGPS
Gene Cloud
IGTC
Mouse Phenome DB
Mouse Mine
KOMP Phenotyping
Monarch
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ClinGen
Slk
Name: STE20-like kinase
Synonyms: 9A2, mSLK, Etk4, SLK, Stk2
Type: Gene
Species: Mouse
Chromosome: 19
NCBI: 20874
Homologene: 22515

Additional Resources
IMPC
Google
Entrez
PubMed
IMSR
Gene Ontology
BioGPS
Gene Cloud
IGTC
Mouse Phenome DB
Mouse Mine
KOMP Phenotyping
Monarch
GeneCards
ClinGen
Setd2
Name: SET domain containing 2
Synonyms: 4921524K10Rik, KMT3A
Type: Gene
Species: Mouse
Chromosome: 9
NCBI: 235626
Homologene: 56493

Additional Resources
IMPC
Google
Entrez
PubMed
IMSR
Gene Ontology
BioGPS
Gene Cloud
IGTC
Mouse Phenome DB
Mouse Mine
KOMP Phenotyping
Monarch
GeneCards
ClinGen
Ppp1r12a
Name: protein phosphatase 1, regulatory subunit 12A
Synonyms: 1200015F06Rik, Mypt1, D10Ertd625e, 5730577I22Rik
Type: Gene
Species: Mouse
Chromosome: 10
NCBI: 17931
VEGA: 10
HGNC: HGNC:7618
Homologene: 1855

Additional Resources
IMPC
Google
Entrez
PubMed
IMSR
Gene Ontology
BioGPS
Gene Cloud
IGTC
Mouse Phenome DB
Mouse Mine
KOMP Phenotyping
Monarch
GeneCards
ClinGen
Genetic Alterations
ENU-induced transitions at the following base pair locations (GRCm38):
  • T to A, chromosome 1 at 26,684,030 bp
  • G to C, chromosome 1 at 55,697,252 bp
  • G to A, chromosome 1 at 71,647,844 bp
  • A to T, chromosome 1 at 110,925,057 bp
  • T to C, chromosome 1 at 119,932,853 bp
  • A to T, chromosome 1 at 150,744,960 bp
  • T to G, chromosome 1 at 153,221,671 bp
  • T to C, chromosome 2 at 32,082,831 bp
  • G to A, chromosome 2 at 86,255,358 bp
  • T to A, chromosome 2 at 89,157,161 bp
  • T to A, chromosome 2 at 111,527,656 bp
  • G to T, chromosome 2 at 153,924,808 bp
  • A to C, chromosome 2 at 158,611,537 bp
  • A to T, chromosome 2 at 163,502,404 bp
  • C to T, chromosome 3 at 72,958,174 bp
  • T to A, chromosome 3 at 88,351,220 bp
  • T to A, chromosome 4 at 20,042,219 bp
  • T to A, chromosome 4 at 65,156,464 bp
  • T to A, chromosome 4 at 130,774,505 bp
  • T to C, chromosome 4 at 144,393,083 bp
  • T to C, chromosome 5 at 24,504,918 bp
  • A to G, chromosome 5 at 105,811,760 bp
  • T to A, chromosome 5 at 115,450,925 bp
  • C to T, chromosome 5 at 136,133,034 bp
  • A to G, chromosome 6 at 41,851,457 bp
  • A to T, chromosome 6 at 89,976,407 bp
  • T to C, chromosome 6 at 118,751,373 bp
  • T to C, chromosome 7 at 11,006,929 bp
  • C to A, chromosome 7 at 28,288,033 bp
  • G to T, chromosome 7 at 28,840,692 bp
  • A to T, chromosome 7 at 45,326,568 bp
  • T to A, chromosome 8 at 125,135,389 bp
  • C to A, chromosome 9 at 21,583,077 bp
  • A to G, chromosome 9 at 29,411,682 bp
  • T to C, chromosome 9 at 39,318,778 bp
  • T to C, chromosome 9 at 44,382,025 bp
  • A to T, chromosome 9 at 48,392,845 bp
  • C to T, chromosome 9 at 58,907,071 bp
  • A to G, chromosome 9 at 65,516,938 bp
  • T to A, chromosome 9 at 110,550,385 bp
  • T to A, chromosome 10 at 27,053,031 bp
  • T to G, chromosome 10 at 33,139,114 bp
  • T to C, chromosome 10 at 94,188,216 bp
  • T to C, chromosome 10 at 108,262,420 bp
  • A to G, chromosome 10 at 128,516,674 bp
  • T to A, chromosome 11 at 53,772,738 bp
  • A to C, chromosome 11 at 74,269,409 bp
  • A to T, chromosome 11 at 86,773,823 bp
  • T to A, chromosome 11 at 99,772,774 bp
  • G to T, chromosome 11 at 100,374,341 bp
  • T to C, chromosome 11 at 100,889,574 bp
  • A to T, chromosome 11 at 121,836,403 bp
  • A to G, chromosome 13 at 22,102,355 bp
  • A to C, chromosome 13 at 93,074,464 bp
  • G to A, chromosome 13 at 109,601,786 bp
  • A to T, chromosome 14 at 50,951,014 bp
  • T to C, chromosome 14 at 59,419,056 bp
  • A to C, chromosome 15 at 28,349,824 bp
  • T to C, chromosome 16 at 29,251,402 bp
  • A to G, chromosome 16 at 35,279,237 bp
  • A to G, chromosome 16 at 38,193,949 bp
  • A to G, chromosome 16 at 65,815,754 bp
  • G to A, chromosome 16 at 74,046,809 bp
  • T to G, chromosome 18 at 31,666,365 bp
  • T to A, chromosome 18 at 74,273,691 bp
  • G to T, chromosome 18 at 80,451,255 bp
  • A to G, chromosome 18 at 80,973,187 bp
  • T to A, chromosome 18 at 84,675,078 bp
  • A to T, chromosome 19 at 39,031,409 bp
  • A to G, chromosome 19 at 47,620,888 bp

Strain Description

Phenotype
G1 male heterozygous mice are viable and fertile. The G1 mice were not characterized prior to archiving.
Strain GQC Summary
Gene Specific Genotyping:

To request gene-specific and other genotyping services for a strain, please contact the distribution MMRRC Center for more information.

Background Genetic Quality:

The MMRRC has developed a Genetic Quality Control pipeline using the MiniMUGA array to provide additional information to identify and validate genetic backgrounds of MMRRC strains. For more information on whether genetic background data is available, please contact MMRRC_GeneticQC@med.unc.edu. Note: that MiniMUGA genetic background data is not available on all strains, but can be ordered if desired.

Suggested Control Mice
Littermates of all relevant genotypes.

Strain Origin

Donor
Bruce Beutler, M.D., University of Texas Southwestern Medical Center.
Primary Reference
MUTAGENETIX record for R6431 (G1). B. Beutler and colleagues, Center for the Genetics of Host Defense, UT Southwestern, Dallas, TX.
Strain Development
C57BL/6J mice were injected with ENU, resulting in G0 mice that were bred back to C57BL/6J. Sperm was archived from G1 male mice that produced viable G2 mice.


Disclaimer: If MMRRC Strain Genetic Quality Control (GQC; based on MiniMUGA genotyping and analysis) has been completed for this strain, the information might differ from the genetic background information provided by the submitter. MiniMUGA genetic analysis is done on a strain’s tissue samples taken when archived by or ordered from the assigned MMRRC Center.

Colony and Husbandry Information

Health Status Report

Colony Surveillance Program and Current Health Reports

Mice recovered from a cryo-archive will have health surveillance performed on recipient females. Health reports will be provided prior to shipment. If you require additional health status information, please email mmrrc@missouri.edu.

Appearance

Coat Color
Black

Breeding

MMRRC Breeding System
Random intra-strain mating
Breeding Scheme(s)
When maintaining a live colony, heterozygous mice may be bred together, bred with wild-type siblings, or bred with C57BL/6J inbred mice.
Overall Breeding Performance
Undetermined; during development, mating was done with heterozygous males; viability, fertility and other breeding statistics for heterozygous females and homozygotes are unverified, but presumably would be similar to the C57BL/6J background strain

Reproductive Statistics

Average litter size
Undetermined
Recommended wean age
3 weeks

Order Information

Availability Level

Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.

Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

Conditions of Distribution

Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.

The donor or their institution limits the distribution to non-profit institutions only.

Fees

Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.

Click button to Request this one strain. (Use the MMRRC Catalog Search to request more than one strain.)
MMRRC Item # Description Distribution Fee / Unit (US $)
*Shipping & Handling not included*		 Units Notes
044569-MU-RESUS Litter recovered from cryo-archive $5,475.00 / Non-Profit Litter Recovered litter4; additional fees for any special requests.
Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

1 The distribution fee covers the expense of rederiving mice from a live mouse; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

2 An aliquot contains a sufficient number of embryos (in one or more vials or straws and based on the transfer success rate of the MMRRC facility) to transfer into one to three recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.

3 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.

4 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.