Strain Name:
C57BL/6J-MtgxR6444Btlr/Mmmh
Stock Number:
044582-MU
Citation ID:
RRID:MMRRC_044582-MU
Other Names:
R6444 (G1)
Major Collection:

Strain Information

Dysf
Name: dysferlin
Synonyms: 2310004N10Rik
Type: Gene
Species: Mouse
Chromosome: 6
NCBI: 26903
HGNC: HGNC:3097
Homologene: 20748
Mybl1
Name: myeloblastosis oncogene-like 1
Synonyms: A-myb, repro9, G1-419-6
Type: Gene
Species: Mouse
Chromosome: 1
NCBI: 17864
HGNC: HGNC:7547
Homologene: 32045
Ptpn3
Name: protein tyrosine phosphatase, non-receptor type 3
Synonyms: 9530011I20Rik, PTPCL, PTP-H1
Type: Gene
Species: Mouse
Chromosome: 4
NCBI: 545622
HGNC: HGNC:9655
Homologene: 74451
Cnot2
Name: CCR4-NOT transcription complex, subunit 2
Synonyms: 2600016M12Rik, 2810470K03Rik
Type: Gene
Species: Mouse
Chromosome: 10
NCBI: 72068
HGNC: HGNC:7878
Homologene: 40953
Zfp568
Name: zinc finger protein 568
Synonyms: chato, LOC381866
Type: Gene
Species: Mouse
Chromosome: 7
NCBI: 243905
Homologene: 136307
Ttc17
Name: tetratricopeptide repeat domain 17
Synonyms: D2Bwg1005e, 9130020K17Rik
Type: Gene
Species: Mouse
Chromosome: 2
NCBI: 74569
Homologene: 10100
Rufy3
Name: RUN and FYVE domain containing 3
Synonyms: 2810428M05Rik, Rpipx, D5Bwg0860e, 6330416M07Rik
Type: Gene
Species: Mouse
Chromosome: 5
NCBI: 52822
Homologene: 87809
Tacc2
Name: transforming, acidic coiled-coil containing protein 2
Type: Gene
Species: Mouse
Chromosome: 7
NCBI: 57752
Homologene: 5087
Miga1
Name: mitoguardin 1
Synonyms: C030011O14Rik, Mita1, Fam73a
Type: Gene
Species: Mouse
Chromosome: 3
NCBI: 215708
Homologene: 18369
Psmd12
Name: proteasome (prosome, macropain) 26S subunit, non-ATPase, 12
Synonyms: P55, 1500002F15Rik
Type: Gene
Species: Mouse
Chromosome: 11
NCBI: 66997
HGNC: HGNC:9557
Homologene: 2109
Fntb
Name: farnesyltransferase, CAAX box, beta
Synonyms: 2010013E13Rik
Type: Gene
Species: Mouse
Chromosome: 12
NCBI: 110606
Homologene: 1535
Znhit1
Name: zinc finger, HIT domain containing 1
Synonyms: 2700001K05Rik
Type: Gene
Species: Mouse
Chromosome: 5
NCBI: 70103
Homologene: 4630
Chd2
Name: chromodomain helicase DNA binding protein 2
Synonyms: 5630401D06Rik, 2810013C04Rik, 2810040A01Rik
Type: Gene
Species: Mouse
Chromosome: 7
NCBI: 244059
HGNC: HGNC:1917
Homologene: 37462
Magel2
Name: MAGE family member L2
Synonyms: ns7, NDNL1, nM15, Mage-l2
Type: Gene
Species: Mouse
Chromosome: 7
NCBI: 27385
HGNC: HGNC:6814
Homologene: 8460
Myo19
Name: myosin XIX
Synonyms: 1110055A02Rik, Myohd1
Type: Gene
Species: Mouse
Chromosome: 11
NCBI: 66196
Homologene: 49819
Ncbp2
Name: nuclear cap binding protein subunit 2
Synonyms: 20kDa
Type: Gene
Species: Mouse
Chromosome: 16
NCBI: 68092
Homologene: 56377
Eif5b
Name: eukaryotic translation initiation factor 5B
Synonyms: IF2, A030003E17Rik
Type: Gene
Species: Mouse
Chromosome: 1
NCBI: 226982
Homologene: 134613
Camk1d
Name: calcium/calmodulin-dependent protein kinase ID
Synonyms: CKLiK, E030025C11Rik, CaMKIdelta, A630059D12Rik
Type: Gene
Species: Mouse
Chromosome: 2
NCBI: 227541
Homologene: 121858
Galns
Name: galactosamine (N-acetyl)-6-sulfatase
Synonyms: N-acetylgalactosamine-6-sulfate sulfatase
Type: Gene
Species: Mouse
Chromosome: 8
NCBI: 50917
HGNC: HGNC:4122
Homologene: 55468
Dscam
Name: DS cell adhesion molecule
Synonyms: Down syndrome cell adhesion molecule, 4932410A21Rik
Type: Gene
Species: Mouse
Chromosome: 16
NCBI: 13508
HGNC: HGNC:3039
Homologene: 74393
Cped1
Name: cadherin-like and PC-esterase domain containing 1
Synonyms: A430107O13Rik
Type: Gene
Species: Mouse
Chromosome: 6
NCBI: 214642
Homologene: 57014
Myo1h
Name: myosin 1H
Synonyms: 4631401O15Rik
Type: Gene
Species: Mouse
Chromosome: 5
NCBI: 231646
Homologene: 82639
Spsb3
Name: splA/ryanodine receptor domain and SOCS box containing 3
Synonyms: SSB3, 3300001M01Rik, Tce1, 2310012N15Rik
Type: Gene
Species: Mouse
Chromosome: 17
NCBI: 79043
Homologene: 12322
Trim75
Name: tripartite motif-containing 75
Synonyms: LOC333307
Type: Gene
Species: Mouse
Chromosome: 8
NCBI: 333307
Homologene: 53452
Slc14a2
Name: solute carrier family 14 (urea transporter), member 2
Synonyms: UT-A5, UT-A3
Type: Gene
Species: Mouse
Chromosome: 18
NCBI: 27411
VEGA: 18
Homologene: 5183
A430033K04Rik
Name: RIKEN cDNA A430033K04 gene
Type: Gene
Species: Mouse
Chromosome: 5
NCBI: 243308
Homologene: 65621
Or8g20
Name: olfactory receptor family 8 subfamily G member 20
Synonyms: IB3, Olfr44, MOR171-5, GA_x6K02T2PVTD-33181773-33180838
Type: Gene
Species: Mouse
Chromosome: 9
NCBI: 18343
VEGA: 9
HGNC: HGNC:8484
Homologene: 133633
Mrgprx1
Name: MAS-related GPR, member X1
Synonyms: MrgC11, Mrgprc11
Type: Gene
Species: Mouse
Chromosome: 7
NCBI: 404242
Homologene: 86184
Galnt15
Name: polypeptide N-acetylgalactosaminyltransferase 15
Synonyms: 4631401E18Rik, Galntl2
Type: Gene
Species: Mouse
Chromosome: 14
NCBI: 78754
Homologene: 14286
Or5b119
Name: olfactory receptor family 5 subfamily B member 119
Synonyms: Olfr1475, MOR202-36, GA_x6K02T2RE5P-3812807-3811863
Type: Gene
Species: Mouse
Chromosome: 19
NCBI: 258298
Homologene: 77370
Trim56
Name: tripartite motif-containing 56
Synonyms: A130009K11Rik, RNF109, LOC384309
Type: Gene
Species: Mouse
Chromosome: 5
NCBI: 384309
Homologene: 12812
Dcdc2c
Name: doublecortin domain containing 2C
Synonyms: 1110015M06Rik
Type: Gene
Species: Mouse
Chromosome: 12
NCBI: 68511
Homologene: 131934
Ydjc
Name: YdjC homolog (bacterial)
Synonyms: 4930521M19Rik, 1810015A11Rik
Type: Gene
Species: Mouse
Chromosome: 16
NCBI: 69101
Homologene: 19062
Bcas2
Name: BCAS2 pre-mRNA processing factor
Synonyms: C76366, breast carcinoma amplified sequence 2, 6430539P16Rik
Type: Gene
Species: Mouse
Chromosome: 3
NCBI: 68183
HGNC: HGNC:975
Homologene: 4291
Smok2a
Name: sperm motility kinase 2A
Synonyms: Smok2
Type: Gene
Species: Mouse
Chromosome: 17
NCBI: 27263
Homologene: 128738
Tmem185b
Name: transmembrane protein 185B
Synonyms: 2500001K11Rik
Type: Gene
Species: Mouse
Chromosome: 1
NCBI: 226351
Homologene: 15760
Gm8229
Name: predicted gene 8229
Type: Gene
Species: Mouse
Chromosome: 14
NCBI: 666675
Homologene: 128452
Genetic Alterations
ENU-induced transitions at the following base pair locations (GRCm38):
  • G to A, chromosome 1 at 9,685,692 bp
  • G to A, chromosome 1 at 38,036,211 bp
  • C to A, chromosome 1 at 119,526,635 bp
  • A to T, chromosome 2 at 5,313,145 bp
  • A to C, chromosome 2 at 94,303,546 bp
  • T to A, chromosome 3 at 103,172,046 bp
  • A to T, chromosome 3 at 152,283,831 bp
  • T to C, chromosome 4 at 57,195,730 bp
  • A to G, chromosome 5 at 88,637,307 bp
  • A to G, chromosome 5 at 114,314,956 bp
  • A to G, chromosome 5 at 136,982,400 bp
  • A to G, chromosome 5 at 137,112,616 bp
  • ACAGAGCAGTGCCTACCAG to ACAG, chromosome 5 at 138,639,569 bp
  • A to C, chromosome 6 at 21,986,931 bp
  • G to A, chromosome 6 at 84,190,840 bp
  • A to T, chromosome 7 at 30,017,257 bp
  • C to T, chromosome 7 at 48,021,814 bp
  • T to C, chromosome 7 at 62,379,999 bp
  • T to C, chromosome 7 at 73,501,037 bp
  • T to C, chromosome 7 at 130,623,412 bp
  • T to C, chromosome 8 at 64,982,836 bp
  • T to C, chromosome 8 at 122,611,338 bp
  • T to C, chromosome 9 at 39,484,318 bp
  • T to C, chromosome 10 at 116,499,355 bp
  • A to G, chromosome 11 at 84,895,308 bp
  • A to G, chromosome 11 at 107,486,454 bp
  • A to G, chromosome 12 at 28,535,476 bp
  • A to G, chromosome 12 at 76,916,440 bp
  • T to C, chromosome 14 at 32,040,411 bp
  • A to T, chromosome 14 at 44,365,471 bp
  • C to A, chromosome 16 at 17,147,681 bp
  • CGTCTGGATG to CG, chromosome 16 at 31,956,343 bp
  • G to A, chromosome 16 at 96,619,644 bp
  • G to T, chromosome 17 at 13,225,613 bp
  • T to A, chromosome 17 at 24,891,576 bp
  • A to G, chromosome 18 at 78,154,102 bp
  • A to T, chromosome 19 at 13,479,430 bp
Phenotype
G1 male heterozygous mice are viable and fertile. The G1 mice were not characterized prior to archiving.
Strain Development
C57BL/6J mice were injected with ENU, resulting in G0 mice that were bred back to C57BL/6J. Sperm was archived from G1 male mice that produced viable G2 mice.
Suggested Control Mice
Wild-type littermates or C57BL/6J mice
MMRRC Genetic QC Summary
The MMRRC Centers have developed a genetic QC pipeline using MiniMUGA array genotyping to provide additional information on strain backgrounds for MMRRC congenic and inbred strains. For more information on when data may be available, or to request genotyping for a strain of interest, please contact mmrrc@missouri.edu. Older strains may not have this information.
Donor
Bruce Beutler, M.D., University of Texas Southwestern Medical Center.
Primary Reference
MUTAGENETIX record for R6444 (G1). B. Beutler and colleagues, Center for the Genetics of Host Defense, UT Southwestern, Dallas, TX.

Colony and Husbandry Information

Colony Surveillance Program and Current Health Reports

Mice recovered from a cryo-archive will have health surveillance performed on recipient females. Health reports will be provided prior to shipment. If you require additional health status information, please email mmrrc@missouri.edu.
Coat Color
Black
MMRRC Breeding System
Random intra-strain mating
Breeding Scheme(s)
When maintaining a live colony, heterozygous mice may be bred together, bred with wild-type siblings, or bred with C57BL/6J inbred mice.
Overall Breeding Performance
Undetermined; during development, mating was done with heterozygous males; viability, fertility and other breeding statistics for heterozygous females and homozygotes are unverified, but presumably would be similar to the C57BL/6J background strain
Average litter size
Undetermined
Recommended wean age
3 weeks

Order Request Information

Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.

Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.

The donor or their institution limits the distribution to non-profit institutions only.

Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.

Click button to Request this one strain. (Use the MMRRC Catalog Search to request more than one strain.)
MMRRC Item # Description Distribution Fee / Unit (US $)
*Shipping & Handling not included*
Units Notes
044582-MU-RESUS Litter recovered from cryo-archive $5,475.00 / Non-Profit Litter Recovered litter4; additional fees for any special requests.
Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

1 The distribution fee covers the expense of rederiving mice from a live mouse; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

2 An aliquot contains a sufficient number of embryos (in one or more vials or straws and based on the transfer success rate of the MMRRC facility) to transfer into one to three recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.

3 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.

4 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.