Strain Name:
C57BL/6J-MtgxR6458Btlr/Mmmh
Stock Number:
044593-MU
Citation ID:
RRID:MMRRC_044593-MU
Other Names:
R6458 (G1)
Major Collection:

Strain Information

Lrp2
Name: low density lipoprotein receptor-related protein 2
Synonyms: b2b1625.2Clo, Gp330, Megalin, D230004K18Rik
Type: Gene
Species: Mouse
Chromosome: 2
NCBI: 14725
HGNC: HGNC:6694
Homologene: 20952
Chd1
Name: chromodomain helicase DNA binding protein 1
Synonyms: 4930525N21Rik
Type: Gene
Species: Mouse
Chromosome: 17
NCBI: 12648
HGNC: HGNC:1915
Homologene: 68174
Cxcr4
Name: C-X-C motif chemokine receptor 4
Synonyms: fusin, CD184, Cmkar4, PB-CKR, Sdf1r, b2b220Clo
Type: Gene
Species: Mouse
Chromosome: 1
NCBI: 12767
HGNC: HGNC:2561
Homologene: 20739
Srek1
Name: splicing regulatory glutamine/lysine-rich protein 1
Synonyms: AL118220, SRrp86, Sfrs12, SRrp508
Type: Gene
Species: Mouse
Chromosome: 13
NCBI: 218543
Homologene: 10581
Nckap1
Name: NCK-associated protein 1
Synonyms: Hem-2, Hem2, Nap1, mh19, H19
Type: Gene
Species: Mouse
Chromosome: 2
NCBI: 50884
HGNC: HGNC:7666
Homologene: 8384
Ralgapb
Name: Ral GTPase activating protein, beta subunit (non-catalytic)
Synonyms: B230339M05Rik
Type: Gene
Species: Mouse
Chromosome: 2
NCBI: 228850
Homologene: 10666
Akap12
Name: A kinase anchor protein 12
Synonyms: Tsga12, Srcs5, SSeCKS
Type: Gene
Species: Mouse
Chromosome: 10
NCBI: 83397
VEGA: 10
HGNC: HGNC:370
Homologene: 3740
Thoc1
Name: THO complex 1
Synonyms: NMP-84, 3110002N20Rik
Type: Gene
Species: Mouse
Chromosome: 18
NCBI: 225160
VEGA: 18
Homologene: 38012
Zfp516
Name: zinc finger protein 516
Synonyms: C330029B10Rik, Zfp26l
Type: Gene
Species: Mouse
Chromosome: 18
NCBI: 329003
VEGA: 18
Homologene: 37122
Slc30a6
Name: solute carrier family 30 (zinc transporter), member 6
Synonyms: ZnT6, ZnT-6
Type: Gene
Species: Mouse
Chromosome: 17
NCBI: 210148
VEGA: 17
Homologene: 32380
Nek1
Name: NIMA (never in mitosis gene a)-related expressed kinase 1
Synonyms: kat, D8Ertd790e, kidney, anemia and testis
Type: Gene
Species: Mouse
Chromosome: 8
NCBI: 18004
HGNC: HGNC:7744
Homologene: 14376
Arel1
Name: apoptosis resistant E3 ubiquitin protein ligase 1
Synonyms: 1110018G07Rik
Type: Gene
Species: Mouse
Chromosome: 12
NCBI: 68497
Homologene: 8885
Fnbp1l
Name: formin binding protein 1-like
Synonyms: 2610318I01Rik, TOCA1
Type: Gene
Species: Mouse
Chromosome: 3
NCBI: 214459
Homologene: 134636
Epg5
Name: ectopic P-granules 5 autophagy tethering factor
Synonyms: 5430411K18Rik
Type: Gene
Species: Mouse
Chromosome: 18
NCBI: 100502841
VEGA: 18
Homologene: 14575
Clca4b
Name: chloride channel accessory 4B
Synonyms: AI747448
Type: Gene
Species: Mouse
Chromosome: 3
NCBI: 99709
HGNC: HGNC:2018
Homologene: 40808
Muc16
Name: mucin 16
Synonyms: LOC385009, 1110008I14Rik, Gm21044
Type: Gene
Species: Mouse
Chromosome: 9
NCBI: 73732
Homologene: 141193
Col4a4
Name: collagen, type IV, alpha 4
Synonyms: E130010M05Rik, [a]4(IV)
Type: Gene
Species: Mouse
Chromosome: 1
NCBI: 12829
HGNC: HGNC:2206
Homologene: 20071
Nbas
Name: neuroblastoma amplified sequence
Synonyms: 4933425L03Rik
Type: Gene
Species: Mouse
Chromosome: 12
NCBI: 71169
VEGA: 12
Homologene: 41073
Anapc4
Name: anaphase promoting complex subunit 4
Synonyms: 2610306D21Rik, APC4, D5Ertd249e
Type: Gene
Species: Mouse
Chromosome: 5
NCBI: 52206
Homologene: 40873
Ttn
Name: titin
Synonyms: 2310036G12Rik, 2310057K23Rik, D330041I19Rik, mdm, connectin, D830007G01Rik, 1100001C23Rik, shru, 2310074I15Rik, L56
Type: Gene
Species: Mouse
Chromosome: 2
NCBI: 22138
Homologene: 130650
Dnah10
Name: dynein, axonemal, heavy chain 10
Synonyms: Dnahc10
Type: Gene
Species: Mouse
Chromosome: 5
NCBI: 56087
HGNC: HGNC:2941
Homologene: 25816
Muc4
Name: mucin 4
Synonyms: 4933405I11Rik
Type: Gene
Species: Mouse
Chromosome: 16
NCBI: 140474
HGNC: HGNC:7514
Homologene: 124469
Ermap
Name: erythroblast membrane-associated protein
Type: Gene
Species: Mouse
Chromosome: 4
NCBI: 27028
Homologene: 22782
Vmn1r69
Name: vomeronasal 1 receptor 69
Synonyms: V1re9
Type: Gene
Species: Mouse
Chromosome: 7
NCBI: 252904
Homologene: 120153
Ihh
Name: Indian hedgehog
Type: Gene
Species: Mouse
Chromosome: 1
NCBI: 16147
HGNC: HGNC:5956
Homologene: 22586
Myt1l
Name: myelin transcription factor 1-like
Synonyms: 2900046C06Rik, C630034G21Rik, Png-1, 2900093J19Rik, Nztf1, Pmng1
Type: Gene
Species: Mouse
Chromosome: 12
NCBI: 17933
VEGA: 12
HGNC: HGNC:7623
Homologene: 7435
Tmc5
Name: transmembrane channel-like gene family 5
Synonyms: 4932443L08Rik
Type: Gene
Species: Mouse
Chromosome: 7
NCBI: 74424
Homologene: 11713
Ttc41
Name: tetratricopeptide repeat domain 41
Synonyms: Gnn, BC030307
Type: Gene
Species: Mouse
Chromosome: 10
NCBI: 103220
VEGA: 10
Homologene: 52968
Vmn2r56
Name: vomeronasal 2, receptor 56
Synonyms: EG629079
Type: Gene
Species: Mouse
Chromosome: 7
NCBI: 629079
Homologene: 104040
Stox2
Name: storkhead box 2
Synonyms: 4933409N07Rik
Type: Gene
Species: Mouse
Chromosome: 8
NCBI: 71069
Homologene: 18414
Gadl1
Name: glutamate decarboxylase-like 1
Synonyms: 1110027M19Rik
Type: Gene
Species: Mouse
Chromosome: 9
NCBI: 73748
Homologene: 45812
Rgma
Name: repulsive guidance molecule family member A
Synonyms: RGM domain family, member A
Type: Gene
Species: Mouse
Chromosome: 7
NCBI: 244058
Homologene: 10626
Dgkq
Name: diacylglycerol kinase, theta
Synonyms: DAGK7, Dagk4, 110kDa, Dgk theta, Dgkd
Type: Gene
Species: Mouse
Chromosome: 5
NCBI: 110524
HGNC: HGNC:2856
Homologene: 20352
Zfp800
Name: zinc finger protein 800
Type: Gene
Species: Mouse
Chromosome: 6
NCBI: 627049
Homologene: 18529
Thoc2l
Name: THO complex subunit 2-like
Synonyms: BC005561, Gm3179
Type: Gene
Species: Mouse
Chromosome: 5
NCBI: 100042165
Ppm1j
Name: protein phosphatase 1J
Synonyms: 2310008J22Rik, Ppp2cz, PP2Czeta
Type: Gene
Species: Mouse
Chromosome: 3
NCBI: 71887
Homologene: 23632
Or8b40
Name: olfactory receptor family 8 subfamily B member 40
Synonyms: MOR162-2, GA_x6K02T2PVTD-31795028-31795957, Olfr889
Type: Gene
Species: Mouse
Chromosome: 9
NCBI: 258475
Homologene: 138311
Mical1
Name: microtubule associated monooxygenase, calponin and LIM domain containing 1
Synonyms: Nical
Type: Gene
Species: Mouse
Chromosome: 10
NCBI: 171580
Homologene: 11246
Bco1
Name: beta-carotene oxygenase 1
Synonyms: Cmoi, Bcmo1, Bcdo, beta-CD, Bcdo1, betaCMOOX
Type: Gene
Species: Mouse
Chromosome: 8
NCBI: 63857
Homologene: 41172
Mpp2
Name: membrane protein, palmitoylated 2 (MAGUK p55 subfamily member 2)
Synonyms: Dlgh2, Pals4, Dlg2, D11Bwg0652e
Type: Gene
Species: Mouse
Chromosome: 11
NCBI: 50997
HGNC: HGNC:7220
Homologene: 3920
Il18r1
Name: interleukin 18 receptor 1
Synonyms: Il18ralpha, Il1rrp
Type: Gene
Species: Mouse
Chromosome: 1
NCBI: 16182
HGNC: HGNC:5988
Homologene: 2861
Ston1
Name: stonin 1
Synonyms: 4921524J06Rik
Type: Gene
Species: Mouse
Chromosome: 17
NCBI: 77057
Homologene: 122152
Lingo3
Name: leucine rich repeat and Ig domain containing 3
Synonyms: LERN2
Type: Gene
Species: Mouse
Chromosome: 10
NCBI: 237403
VEGA: 10
Homologene: 78065
Or14c43
Name: olfactory receptor family 14 subfamily C member 43
Synonyms: GA_x6K02T2NHDJ-9643949-9642957, Olfr299, MOR221-2
Type: Gene
Species: Mouse
Chromosome: 7
NCBI: 257929
Homologene: 115489
Ak1
Name: adenylate kinase 1
Synonyms: B430205N08Rik, Ak-1
Type: Gene
Species: Mouse
Chromosome: 2
NCBI: 11636
HGNC: HGNC:361
Homologene: 20135
H2-T3
Name: histocompatibility 2, T region locus 3
Synonyms: H-2T3, TL, H2-Tw3
Type: Gene
Species: Mouse
Chromosome: 17
NCBI: 15043
Homologene: 74884
Gm5591
Name: predicted gene 5591
Type: Gene
Species: Mouse
Chromosome: 7
NCBI: 434171
Homologene: 80005
Acot8
Name: acyl-CoA thioesterase 8
Synonyms: Pte1, PTE-2
Type: Gene
Species: Mouse
Chromosome: 2
NCBI: 170789
Homologene: 3991
Ccr1l1
Name: C-C motif chemokine receptor 1 like 1
Synonyms: MIP-1 alphaRL1, Cmkbr1l1
Type: Gene
Species: Mouse
Chromosome: 9
NCBI: 12770
VEGA: 9
HGNC: HGNC:1602
Homologene: 56388
Zpbp
Name: zona pellucida binding protein
Synonyms: Sp38, 4930486K01Rik
Type: Gene
Species: Mouse
Chromosome: 11
NCBI: 53604
Homologene: 5092
Or4p22
Name: olfactory receptor family 4 subfamily P member 22
Synonyms: MOR225-3, Olfr1184, GA_x6K02T2Q125-49974190-49975125
Type: Gene
Species: Mouse
Chromosome: 2
NCBI: 258820
Homologene: 73980
Spr
Name: sepiapterin reductase
Type: Gene
Species: Mouse
Chromosome: 6
NCBI: 20751
Homologene: 37735
Or1e18-ps1
Name: olfactory receptor family 1 subfamily E member 18, pseudogene 1
Synonyms: GA_x6K02T2P1NL-3549348-3548405, MOR135-15, Olfr377-ps1
Type: Gene
Species: Mouse
Chromosome: 11
NCBI: 258226
Genetic Alterations
ENU-induced transitions at the following base pair locations (GRCm38):
  • T to A, chromosome 1 at 40,491,182 bp
  • C to T, chromosome 1 at 74,946,442 bp
  • T to C, chromosome 1 at 82,455,825 bp
  • T to C, chromosome 1 at 128,589,094 bp
  • T to A, chromosome 2 at 32,630,373 bp
  • T to G, chromosome 2 at 69,505,156 bp
  • A to T, chromosome 2 at 76,778,510 bp
  • T to C, chromosome 2 at 80,512,549 bp
  • T to A, chromosome 2 at 88,487,218 bp
  • T to A, chromosome 2 at 158,444,620 bp
  • A to G, chromosome 2 at 164,804,735 bp
  • T to C, chromosome 3 at 104,781,244 bp
  • A to T, chromosome 3 at 122,556,440 bp
  • T to G, chromosome 3 at 144,911,327 bp
  • A to T, chromosome 4 at 119,178,140 bp
  • G to A, chromosome 5 at 52,864,553 bp
  • A to T, chromosome 5 at 104,522,303 bp
  • A to G, chromosome 5 at 108,654,376 bp
  • G to T, chromosome 5 at 124,809,269 bp
  • A to T, chromosome 6 at 28,244,216 bp
  • T to C, chromosome 6 at 85,137,057 bp
  • A to G, chromosome 7 at 10,580,438 bp
  • T to A, chromosome 7 at 12,694,057 bp
  • T to C, chromosome 7 at 24,228,445 bp
  • T to A, chromosome 7 at 38,519,035 bp
  • T to A, chromosome 7 at 73,409,694 bp
  • A to G, chromosome 7 at 86,465,680 bp
  • T to A, chromosome 7 at 118,645,316 bp
  • T to C, chromosome 8 at 47,192,044 bp
  • T to G, chromosome 8 at 61,100,012 bp
  • A to G, chromosome 8 at 117,127,506 bp
  • A to T, chromosome 9 at 18,641,721 bp
  • T to C, chromosome 9 at 38,116,054 bp
  • T to A, chromosome 9 at 116,041,002 bp
  • A to T, chromosome 9 at 123,978,166 bp
  • T to C, chromosome 10 at 4,355,148 bp
  • C to A, chromosome 10 at 41,484,735 bp
  • A to G, chromosome 10 at 80,835,316 bp
  • A to G, chromosome 10 at 86,758,270 bp
  • T to A, chromosome 11 at 11,408,538 bp
  • T to G, chromosome 11 at 73,388,691 bp
  • T to A, chromosome 11 at 102,080,769 bp
  • T to C, chromosome 12 at 13,288,749 bp
  • T to A, chromosome 12 at 29,895,299 bp
  • T to C, chromosome 12 at 84,940,385 bp
  • A to T, chromosome 13 at 103,743,568 bp
  • T to A, chromosome 16 at 32,759,320 bp
  • A to T, chromosome 17 at 15,730,602 bp
  • A to T, chromosome 17 at 36,187,019 bp
  • A to G, chromosome 17 at 74,423,113 bp
  • A to T, chromosome 17 at 88,635,303 bp
  • G to A, chromosome 18 at 9,993,333 bp
  • T to A, chromosome 18 at 77,948,254 bp
  • A to G, chromosome 18 at 82,987,350 bp
Phenotype
G1 male heterozygous mice are viable and fertile. The G1 mice were not characterized prior to archiving.
Strain Development
C57BL/6J mice were injected with ENU, resulting in G0 mice that were bred back to C57BL/6J. Sperm was archived from G1 male mice that produced viable G2 mice.
Suggested Control Mice
Wild-type littermates or C57BL/6J mice
MMRRC Genetic QC Summary
The MMRRC Centers have developed a genetic QC pipeline using MiniMUGA array genotyping to provide additional information on strain backgrounds for MMRRC congenic and inbred strains. For more information on when data may be available, or to request genotyping for a strain of interest, please contact mmrrc@missouri.edu. Older strains may not have this information.
Donor
Bruce Beutler, M.D., University of Texas Southwestern Medical Center.
Primary Reference
MUTAGENETIX record for R6458 (G1). B. Beutler and colleagues, Center for the Genetics of Host Defense, UT Southwestern, Dallas, TX.

Colony and Husbandry Information

Colony Surveillance Program and Current Health Reports

Mice recovered from a cryo-archive will have health surveillance performed on recipient females. Health reports will be provided prior to shipment. If you require additional health status information, please email mmrrc@missouri.edu.
Coat Color
Black
MMRRC Breeding System
Random intra-strain mating
Breeding Scheme(s)
When maintaining a live colony, heterozygous mice may be bred together, bred with wild-type siblings, or bred with C57BL/6J inbred mice.
Overall Breeding Performance
Undetermined; during development, mating was done with heterozygous males; viability, fertility and other breeding statistics for heterozygous females and homozygotes are unverified, but presumably would be similar to the C57BL/6J background strain
Average litter size
Undetermined
Recommended wean age
3 weeks

Order Request Information

Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.

Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.

The donor or their institution limits the distribution to non-profit institutions only.

Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.

Click button to Request this one strain. (Use the MMRRC Catalog Search to request more than one strain.)
MMRRC Item # Description Distribution Fee / Unit (US $)
*Shipping & Handling not included*
Units Notes
044593-MU-RESUS Litter recovered from cryo-archive $5,475.00 / Non-Profit Litter Recovered litter4; additional fees for any special requests.
Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

1 The distribution fee covers the expense of rederiving mice from a live mouse; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

2 An aliquot contains a sufficient number of embryos (in one or more vials or straws and based on the transfer success rate of the MMRRC facility) to transfer into one to three recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.

3 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.

4 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.