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Strain Name:
C57BL/6J-MtgxR6466Btlr/Mmmh
Stock Number:
044599-MU
Citation ID:
RRID:MMRRC_044599-MU
Other Names:
R6466 (G1)
Major Collection:

Strain Information

Nfat5
Name: nuclear factor of activated T cells 5
Synonyms: nfatz, TonEBP, B130038B15Rik, OREBP
Type: Gene
Species: Mouse
Chromosome: 8
NCBI: 54446
HGNC: HGNC:7774
Homologene: 4811
Qki
Name: quaking, KH domain containing RNA binding
Synonyms: l(17)-1Wis, QkI, l17Wis1, 1110003F05Rik, Qk
Type: Gene
Species: Mouse
Chromosome: 17
NCBI: 19317
VEGA: 17
Homologene: 11059
Chrm1
Name: cholinergic receptor, muscarinic 1, CNS
Synonyms: M1, muscarinic acetylcholine receptor 1, Chrm-1, M1R, AW495047
Type: Gene
Species: Mouse
Chromosome: 19
NCBI: 12669
HGNC: HGNC:1950
Homologene: 20189
Bpifb3
Name: BPI fold containing family B, member 3
Synonyms: Rya3
Type: Gene
Species: Mouse
Chromosome: 2
NCBI: 378700
Homologene: 18807
Slco1a5
Name: solute carrier organic anion transporter family, member 1a5
Synonyms: Oatp3, Slc21a7
Type: Gene
Species: Mouse
Chromosome: 6
NCBI: 108096
Homologene: 56603
1190005I06Rik
Name: RIKEN cDNA 1190005I06 gene
Type: Gene
Species: Mouse
Chromosome: 8
NCBI: 68918
Homologene: 18858
Genetic Alterations
ENU-induced transitions at the following base pair locations (GRCm38):
  • T to C, chromosome 1 at 4,347,886 bp
  • G to T, chromosome 1 at 163,954,165 bp
  • C to A, chromosome 1 at 171,386,753 bp
  • T to A, chromosome 1 at 174,609,583 bp
  • A to G, chromosome 1 at 174,836,884 bp
  • G to T, chromosome 2 at 118,119,847 bp
  • G to T, chromosome 2 at 153,922,188 bp
  • A to T, chromosome 3 at 92,353,034 bp
  • T to C, chromosome 4 at 11,252,878 bp
  • A to G, chromosome 4 at 88,721,162 bp
  • T to A, chromosome 4 at 144,703,867 bp
  • T to A, chromosome 4 at 145,057,495 bp
  • C to T, chromosome 5 at 29,378,168 bp
  • A to G, chromosome 5 at 112,461,141 bp
  • T to C, chromosome 6 at 70,699,039 bp
  • G to T, chromosome 6 at 130,179,032 bp
  • T to C, chromosome 6 at 142,237,534 bp
  • A to T, chromosome 7 at 31,143,796 bp
  • A to G, chromosome 7 at 75,727,044 bp
  • A to C, chromosome 7 at 85,407,170 bp
  • A to G, chromosome 7 at 100,100,632 bp
  • A to T, chromosome 7 at 105,764,541 bp
  • A to G, chromosome 7 at 107,814,694 bp
  • A to T, chromosome 8 at 48,236,063 bp
  • A to C, chromosome 8 at 60,929,561 bp
  • A to G, chromosome 8 at 71,557,849 bp
  • A to G, chromosome 8 at 92,359,726 bp
  • T to A, chromosome 8 at 107,355,508 bp
  • T to A, chromosome 8 at 110,506,968 bp
  • A to T, chromosome 8 at 120,608,996 bp
  • G to T, chromosome 8 at 125,672,384 bp
  • T to C, chromosome 9 at 48,665,319 bp
  • A to T, chromosome 11 at 21,328,883 bp
  • T to A, chromosome 11 at 69,539,415 bp
  • A to C, chromosome 11 at 83,429,353 bp
  • T to A, chromosome 11 at 102,439,885 bp
  • A to T, chromosome 12 at 35,504,032 bp
  • A to G, chromosome 12 at 75,943,901 bp
  • G to T, chromosome 13 at 81,575,101 bp
  • C to T, chromosome 14 at 20,695,309 bp
  • A to G, chromosome 14 at 62,948,666 bp
  • A to G, chromosome 14 at 70,309,886 bp
  • A to G, chromosome 15 at 75,910,503 bp
  • A to G, chromosome 15 at 76,177,884 bp
  • T to C, chromosome 15 at 99,238,243 bp
  • C to A, chromosome 16 at 25,763,358 bp
  • A to G, chromosome 16 at 97,879,622 bp
  • T to C, chromosome 17 at 5,327,678 bp
  • T to C, chromosome 17 at 10,215,465 bp
  • G to T, chromosome 17 at 20,960,631 bp
  • C to T, chromosome 17 at 56,784,397 bp
  • T to A, chromosome 19 at 6,928,297 bp
  • T to A, chromosome 19 at 8,678,178 bp
  • G to T, chromosome 19 at 8,709,319 bp
Phenotype
G1 male heterozygous mice are viable and fertile. The G1 mice were not characterized prior to archiving.
Strain GQC Summary
The MMRRC Centers have developed a Strain GQC pipeline using MiniMUGA array genotyping to provide additional information on strain backgrounds for MMRRC strains. For more information on whether data may be available, or to request genotyping for a strain of interest, please contact MMRRC_GeneticQC@med.unc.edu. Older strains may not have this information available.
Suggested Control Mice
Littermates of all relevant genotypes.
Donor
Bruce Beutler, M.D., University of Texas Southwestern Medical Center.
Primary Reference
MUTAGENETIX record for R6466 (G1). B. Beutler and colleagues, Center for the Genetics of Host Defense, UT Southwestern, Dallas, TX.
Strain Development
C57BL/6J mice were injected with ENU, resulting in G0 mice that were bred back to C57BL/6J. Sperm was archived from G1 male mice that produced viable G2 mice.


Disclaimer: If MMRRC Strain Genetic Quality Control (GQC; based on MiniMUGA genotyping and analysis) has been completed for this strain, the information might differ from the genetic background information provided by the submitter. MiniMUGA genetic analysis is done on a strain’s tissue samples taken when archived by or ordered from the assigned MMRRC Center.

Colony and Husbandry Information

Colony Surveillance Program and Current Health Reports

Mice recovered from a cryo-archive will have health surveillance performed on recipient females. Health reports will be provided prior to shipment. If you require additional health status information, please email mmrrc@missouri.edu.
Coat Color
Black
MMRRC Breeding System
Random intra-strain mating
Breeding Scheme(s)
When maintaining a live colony, heterozygous mice may be bred together, bred with wild-type siblings, or bred with C57BL/6J inbred mice.
Overall Breeding Performance
Undetermined; during development, mating was done with heterozygous males; viability, fertility and other breeding statistics for heterozygous females and homozygotes are unverified, but presumably would be similar to the C57BL/6J background strain
Average litter size
Undetermined
Recommended wean age
3 weeks

Order Request Information

Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.

Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.

The donor or their institution limits the distribution to non-profit institutions only.

Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.

Click button to Request this one strain. (Use the MMRRC Catalog Search to request more than one strain.)
MMRRC Item # Description Distribution Fee / Unit (US $)
*Shipping & Handling not included*
Units Notes
044599-MU-RESUS Litter recovered from cryo-archive $5,475.00 / Non-Profit Litter Recovered litter4; additional fees for any special requests.
Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

1 The distribution fee covers the expense of rederiving mice from a live mouse; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

2 An aliquot contains a sufficient number of embryos (in one or more vials or straws and based on the transfer success rate of the MMRRC facility) to transfer into one to three recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.

3 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.

4 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.