Strain Name:
C57BL/6J-MtgxR6466Btlr/Mmmh
Stock Number:
044599-MU
Citation ID:
RRID:MMRRC_044599-MU
Other Names:
R6466 (G1)
Major Collection:

Strain Information

Nfat5
Name: nuclear factor of activated T cells 5
Synonyms: nfatz, TonEBP, B130038B15Rik, OREBP
Type: Gene
Species: Mouse
Chromosome: 8
NCBI: 54446
HGNC: HGNC:7774
Homologene: 4811
Qki
Name: quaking, KH domain containing RNA binding
Synonyms: l(17)-1Wis, l17Wis1, QkI, 1110003F05Rik, Qk
Type: Gene
Species: Mouse
Chromosome: 17
NCBI: 19317
VEGA: 17
Homologene: 11059
Chrm1
Name: cholinergic receptor, muscarinic 1, CNS
Synonyms: M1, muscarinic acetylcholine receptor 1, Chrm-1, M1R, AW495047
Type: Gene
Species: Mouse
Chromosome: 19
NCBI: 12669
HGNC: HGNC:1950
Homologene: 20189
Bpifb3
Name: BPI fold containing family B, member 3
Synonyms: Rya3
Type: Gene
Species: Mouse
Chromosome: 2
NCBI: 378700
Homologene: 18807
Slco1a5
Name: solute carrier organic anion transporter family, member 1a5
Synonyms: Oatp3, Slc21a7
Type: Gene
Species: Mouse
Chromosome: 6
NCBI: 108096
Homologene: 56603
1190005I06Rik
Name: RIKEN cDNA 1190005I06 gene
Type: Gene
Species: Mouse
Chromosome: 8
NCBI: 68918
Homologene: 18858
Fmn2
Name: formin 2
Type: Gene
Species: Mouse
Chromosome: 1
NCBI: 54418
Homologene: 137334
Akap13
Name: A kinase anchor protein 13
Synonyms: AKAP-Lbc, PROTO-LBC, PROTO-LB, Ht31, 5730522G15Rik, 1700026G02Rik, 5830460E08Rik
Type: Gene
Species: Mouse
Chromosome: 7
NCBI: 75547
HGNC: HGNC:371
Homologene: 4903
Ints8
Name: integrator complex subunit 8
Synonyms: D130008D20Rik, 2810013E07Rik
Type: Gene
Species: Mouse
Chromosome: 4
NCBI: 72656
Homologene: 9888
Arid1b
Name: AT-rich interaction domain 1B
Synonyms: B230217J03Rik, 9330189K18Rik
Type: Gene
Species: Mouse
Chromosome: 17
NCBI: 239985
Homologene: 32344
Ppp2r1a
Name: protein phosphatase 2, regulatory subunit A, alpha
Synonyms: PR65, protein phosphatase PP2A, 6330556D22Rik, PP2A
Type: Gene
Species: Mouse
Chromosome: 17
NCBI: 51792
HGNC: HGNC:9302
Homologene: 68559
Thbs1
Name: thrombospondin 1
Synonyms: TSP1, TSP-1, Thbs-1, tbsp1
Type: Gene
Species: Mouse
Chromosome: 2
NCBI: 21825
Homologene: 31142
Rfx2
Name: regulatory factor X, 2 (influences HLA class II expression)
Synonyms: 5430432H19Rik
Type: Gene
Species: Mouse
Chromosome: 17
NCBI: 19725
VEGA: 17
HGNC: HGNC:9983
Homologene: 30980
Lmbr1
Name: limb region 1
Synonyms: 1110048D14Rik, C79130
Type: Gene
Species: Mouse
Chromosome: 5
NCBI: 56873
Homologene: 49706
Vps13d
Name: vacuolar protein sorting 13D
Type: Gene
Species: Mouse
Chromosome: 4
NCBI: 230895
Homologene: 15583
Slc39a14
Name: solute carrier family 39 (zinc transporter), member 14
Synonyms: G630015O18Rik, Zip14
Type: Gene
Species: Mouse
Chromosome: 14
NCBI: 213053
Homologene: 15037
Zbtb16
Name: zinc finger and BTB domain containing 16
Synonyms: PLZF, Zfp145, Green's luxoid
Type: Gene
Species: Mouse
Chromosome: 9
NCBI: 235320
VEGA: 9
Homologene: 21214
Slc3a2
Name: solute carrier family 3 (activators of dibasic and neutral amino acid transport), member 2
Synonyms: Mgp-2hc, Ly-10, Ly-m10, Mdu1, 4F2HC, Cd98
Type: Gene
Species: Mouse
Chromosome: 19
NCBI: 17254
Homologene: 1795
Tenm3
Name: teneurin transmembrane protein 3
Synonyms: Ten-m3, 2610100B16Rik, Odz3
Type: Gene
Species: Mouse
Chromosome: 8
NCBI: 23965
Homologene: 22673
Pold3
Name: polymerase (DNA-directed), delta 3, accessory subunit
Synonyms: P68, P66, 2410142G14Rik, C85233, GC12
Type: Gene
Species: Mouse
Chromosome: 7
NCBI: 67967
Homologene: 38202
Firrm
Name: FIGNL1 interacting regulator of recombination and mitosis
Synonyms: BC055324, Flip
Type: Gene
Species: Mouse
Chromosome: 1
NCBI: 381306
Homologene: 10058
Sez6l
Name: seizure related 6 homolog like
Synonyms: Acig1
Type: Gene
Species: Mouse
Chromosome: 5
NCBI: 56747
Homologene: 10895
Hydin
Name: HYDIN, axonemal central pair apparatus protein
Synonyms: hy-3, hy3, 1700034M11Rik, 4930545D19Rik, hyrh
Type: Gene
Species: Mouse
Chromosome: 8
NCBI: 244653
Homologene: 52118
AAdacl4fm3
Name: AADACL4 family member 3
Synonyms: Gm13178
Type: Gene
Species: Mouse
Chromosome: 4
NCBI: 546849
Homologene: 82586
Dchs1
Name: dachsous cadherin related 1
Synonyms: C130033F22Rik, 3110041P15Rik
Type: Gene
Species: Mouse
Chromosome: 7
NCBI: 233651
Homologene: 2771
Dnah2
Name: dynein, axonemal, heavy chain 2
Synonyms: D330014H01Rik, 2900022L05Rik, Dnhd3, Dnahc2
Type: Gene
Species: Mouse
Chromosome: 11
NCBI: 327954
HGNC: HGNC:2948
Homologene: 72110
Syne2
Name: spectrin repeat containing, nuclear envelope 2
Synonyms: nesprin-2, syne-2, D12Ertd777e, 6820443O06Rik, Nesp2g, Cpfl8, diminished cone electroretinogram, dice
Type: Gene
Species: Mouse
Chromosome: 12
NCBI: 319565
Homologene: 56700
Clcn3
Name: chloride channel, voltage-sensitive 3
Synonyms: Clc3
Type: Gene
Species: Mouse
Chromosome: 8
NCBI: 12725
HGNC: HGNC:2021
Homologene: 20435
Adgrv1
Name: adhesion G protein-coupled receptor V1
Synonyms: VLGR1, Mass1, Mgr1, Gpr98
Type: Gene
Species: Mouse
Chromosome: 13
NCBI: 110789
Homologene: 19815
Rp1
Name: retinitis pigmentosa 1 (human)
Synonyms: oxygen-regulated protein 1, Orp1, mG145, Dcdc3, Rp1h
Type: Gene
Species: Mouse
Chromosome: 1
NCBI: 19888
Homologene: 4564
Gas2l2
Name: growth arrest-specific 2 like 2
Synonyms: OTTMUSG00000000934
Type: Gene
Species: Mouse
Chromosome: 11
NCBI: 237891
Homologene: 16409
Trp63
Name: transformation related protein 63
Synonyms: KET protein, p63, p73L, Trp53rp1, TAp63, p51/p63, deltaNp63
Type: Gene
Species: Mouse
Chromosome: 16
NCBI: 22061
Homologene: 31189
Fam171a2
Name: family with sequence similarity 171, member A2
Type: Gene
Species: Mouse
Chromosome: 11
NCBI: 217219
Homologene: 19144
Or5p5
Name: olfactory receptor family 5 subfamily P member 5
Synonyms: GA_x6K02T2PBJ9-10144091-10145011, MOR204-33P, Olfr467
Type: Gene
Species: Mouse
Chromosome: 7
NCBI: 257919
Homologene: 81542
Sprr2e
Name: small proline-rich protein 2E
Type: Gene
Species: Mouse
Chromosome: 3
NCBI: 20759
Kcnk4
Name: potassium channel, subfamily K, member 4
Synonyms: TRAAKt
Type: Gene
Species: Mouse
Chromosome: 19
NCBI: 16528
VEGA: 19
HGNC: HGNC:6279
Homologene: 7391
Gramd1a
Name: GRAM domain containing 1A
Synonyms: 1300003M23Rik, D7Bwg0611e
Type: Gene
Species: Mouse
Chromosome: 7
NCBI: 52857
Homologene: 10843
Klhl9
Name: kelch-like 9
Synonyms: C530050O22Rik
Type: Gene
Species: Mouse
Chromosome: 4
NCBI: 242521
Homologene: 23125
Wdfy2
Name: WD repeat and FYVE domain containing 2
Synonyms: B130024L21Rik
Type: Gene
Species: Mouse
Chromosome: 14
NCBI: 268752
VEGA: 14
Homologene: 14188
Klra9
Name: killer cell lectin-like receptor subfamily A, member 9
Synonyms: Ly49I, LY49I1
Type: Gene
Species: Mouse
Chromosome: 6
NCBI: 16640
Homologene: 110821
Kcnh3
Name: potassium voltage-gated channel, subfamily H (eag-related), member 3
Synonyms: ether a go-go like, Elk2, Melk2, C030044P22Rik
Type: Gene
Species: Mouse
Chromosome: 15
NCBI: 16512
VEGA: 15
HGNC: HGNC:6252
Homologene: 32150
C2cd2
Name: C2 calcium-dependent domain containing 2
Synonyms: ORF25, 5830404H04Rik
Type: Gene
Species: Mouse
Chromosome: 16
NCBI: 207781
VEGA: 16
HGNC: HGNC:1266
Homologene: 18368
Vmn2r69
Name: vomeronasal 2, receptor 69
Type: Gene
Species: Mouse
Chromosome: 7
NCBI: 330581
Homologene: 115466
Ahr
Name: aryl-hydrocarbon receptor
Synonyms: dioxin receptor, Ahh, Ah, In, Ahre, bHLHe76
Type: Gene
Species: Mouse
Chromosome: 12
NCBI: 11622
HGNC: HGNC:348
Homologene: 1224
Fut11
Name: fucosyltransferase 11
Synonyms: 3110009G03Rik
Type: Gene
Species: Mouse
Chromosome: 14
NCBI: 73068
VEGA: 14
Homologene: 18313
Irx5
Name: Iroquois homeobox 5
Type: Gene
Species: Mouse
Chromosome: 8
NCBI: 54352
Homologene: 38105
Map10
Name: microtubule-associated protein 10
Synonyms: 4933403G14Rik
Type: Gene
Species: Mouse
Chromosome: 8
NCBI: 74393
Homologene: 10416
Ugp2
Name: UDP-glucose pyrophosphorylase 2
Type: Gene
Species: Mouse
Chromosome: 11
NCBI: 216558
Homologene: 121676
Tmem221
Name: transmembrane protein 221
Type: Gene
Species: Mouse
Chromosome: 8
NCBI: 434325
Homologene: 110174
Nectin4
Name: nectin cell adhesion molecule 4
Synonyms: nectin 4, Prr4, 1200017F15Rik, Pvrl4
Type: Gene
Species: Mouse
Chromosome: 1
NCBI: 71740
Homologene: 32744
Igkv3-2
Name: immunoglobulin kappa variable 3-2
Synonyms: V(kappa)21A
Type: Gene
Species: Mouse
Chromosome: 6
NCBI: 626583
Tigd5
Name: tigger transposable element derived 5
Synonyms: FLJ14926
Type: Gene
Species: Mouse
Chromosome: 15
NCBI: 105734
Homologene: 129878
Grem2
Name: gremlin 2, DAN family BMP antagonist
Synonyms: Prdc
Type: Gene
Species: Mouse
Chromosome: 1
NCBI: 23893
Homologene: 8023
Genetic Alterations
ENU-induced transitions at the following base pair locations (GRCm38):
  • T to C, chromosome 1 at 4,347,886 bp
  • G to T, chromosome 1 at 163,954,165 bp
  • C to A, chromosome 1 at 171,386,753 bp
  • T to A, chromosome 1 at 174,609,583 bp
  • A to G, chromosome 1 at 174,836,884 bp
  • G to T, chromosome 2 at 118,119,847 bp
  • G to T, chromosome 2 at 153,922,188 bp
  • A to T, chromosome 3 at 92,353,034 bp
  • T to C, chromosome 4 at 11,252,878 bp
  • A to G, chromosome 4 at 88,721,162 bp
  • T to A, chromosome 4 at 144,703,867 bp
  • T to A, chromosome 4 at 145,057,495 bp
  • C to T, chromosome 5 at 29,378,168 bp
  • A to G, chromosome 5 at 112,461,141 bp
  • T to C, chromosome 6 at 70,699,039 bp
  • G to T, chromosome 6 at 130,179,032 bp
  • T to C, chromosome 6 at 142,237,534 bp
  • A to T, chromosome 7 at 31,143,796 bp
  • A to G, chromosome 7 at 75,727,044 bp
  • A to C, chromosome 7 at 85,407,170 bp
  • A to G, chromosome 7 at 100,100,632 bp
  • A to T, chromosome 7 at 105,764,541 bp
  • A to G, chromosome 7 at 107,814,694 bp
  • A to T, chromosome 8 at 48,236,063 bp
  • A to C, chromosome 8 at 60,929,561 bp
  • A to G, chromosome 8 at 71,557,849 bp
  • A to G, chromosome 8 at 92,359,726 bp
  • T to A, chromosome 8 at 107,355,508 bp
  • T to A, chromosome 8 at 110,506,968 bp
  • A to T, chromosome 8 at 120,608,996 bp
  • G to T, chromosome 8 at 125,672,384 bp
  • T to C, chromosome 9 at 48,665,319 bp
  • A to T, chromosome 11 at 21,328,883 bp
  • T to A, chromosome 11 at 69,539,415 bp
  • A to C, chromosome 11 at 83,429,353 bp
  • T to A, chromosome 11 at 102,439,885 bp
  • A to T, chromosome 12 at 35,504,032 bp
  • A to G, chromosome 12 at 75,943,901 bp
  • G to T, chromosome 13 at 81,575,101 bp
  • C to T, chromosome 14 at 20,695,309 bp
  • A to G, chromosome 14 at 62,948,666 bp
  • A to G, chromosome 14 at 70,309,886 bp
  • A to G, chromosome 15 at 75,910,503 bp
  • A to G, chromosome 15 at 76,177,884 bp
  • T to C, chromosome 15 at 99,238,243 bp
  • C to A, chromosome 16 at 25,763,358 bp
  • A to G, chromosome 16 at 97,879,622 bp
  • T to C, chromosome 17 at 5,327,678 bp
  • T to C, chromosome 17 at 10,215,465 bp
  • G to T, chromosome 17 at 20,960,631 bp
  • C to T, chromosome 17 at 56,784,397 bp
  • T to A, chromosome 19 at 6,928,297 bp
  • T to A, chromosome 19 at 8,678,178 bp
  • G to T, chromosome 19 at 8,709,319 bp
Phenotype
G1 male heterozygous mice are viable and fertile. The G1 mice were not characterized prior to archiving.
Strain Development
C57BL/6J mice were injected with ENU, resulting in G0 mice that were bred back to C57BL/6J. Sperm was archived from G1 male mice that produced viable G2 mice.
Suggested Control Mice
Wild-type littermates or C57BL/6J mice
MMRRC Genetic QC Summary
The MMRRC Centers have developed a genetic QC pipeline using MiniMUGA array genotyping to provide additional information on strain backgrounds for MMRRC congenic and inbred strains. For more information on when data may be available, or to request genotyping for a strain of interest, please contact mmrrc@missouri.edu. Older strains may not have this information.
Donor
Bruce Beutler, M.D., University of Texas Southwestern Medical Center.
Primary Reference
MUTAGENETIX record for R6466 (G1). B. Beutler and colleagues, Center for the Genetics of Host Defense, UT Southwestern, Dallas, TX.

Colony and Husbandry Information

Colony Surveillance Program and Current Health Reports

Mice recovered from a cryo-archive will have health surveillance performed on recipient females. Health reports will be provided prior to shipment. If you require additional health status information, please email mmrrc@missouri.edu.
Coat Color
Black
MMRRC Breeding System
Random intra-strain mating
Breeding Scheme(s)
When maintaining a live colony, heterozygous mice may be bred together, bred with wild-type siblings, or bred with C57BL/6J inbred mice.
Overall Breeding Performance
Undetermined; during development, mating was done with heterozygous males; viability, fertility and other breeding statistics for heterozygous females and homozygotes are unverified, but presumably would be similar to the C57BL/6J background strain
Average litter size
Undetermined
Recommended wean age
3 weeks

Order Request Information

Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.

Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.

The donor or their institution limits the distribution to non-profit institutions only.

Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.

Click button to Request this one strain. (Use the MMRRC Catalog Search to request more than one strain.)
MMRRC Item # Description Distribution Fee / Unit (US $)
*Shipping & Handling not included*
Units Notes
044599-MU-RESUS Litter recovered from cryo-archive $5,475.00 / Non-Profit Litter Recovered litter4; additional fees for any special requests.
Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

1 The distribution fee covers the expense of rederiving mice from a live mouse; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

2 An aliquot contains a sufficient number of embryos (in one or more vials or straws and based on the transfer success rate of the MMRRC facility) to transfer into one to three recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.

3 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.

4 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.