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Strain Name:
C57BL/6J-MtgxR6469Btlr/Mmmh
Stock Number:
044602-MU
Citation ID:
RRID:MMRRC_044602-MU
Other Names:
R6469 (G1)
Major Collection:

Strain Information

Fgb
Name: fibrinogen beta chain
Synonyms: 2510049G14Rik
Type: Gene
Species: Mouse
Chromosome: 3
NCBI: 110135
HGNC: HGNC:3662
Homologene: 3772
Gba1
Name: glucosylceramidase beta 1
Synonyms: glucocerebrosidase, GC, GCase, GBA1, betaGC, Gba
Type: Gene
Species: Mouse
Chromosome: 3
NCBI: 14466
HGNC: HGNC:4177
Homologene: 68040
Ncor1
Name: nuclear receptor co-repressor 1
Synonyms: N-CoR, Rxrip13, A230020K14Rik, 5730405M06Rik
Type: Gene
Species: Mouse
Chromosome: 11
NCBI: 20185
HGNC: HGNC:7672
Homologene: 38166
Rpl3
Name: ribosomal protein L3
Synonyms: J1, F2
Type: Gene
Species: Mouse
Chromosome: 15
NCBI: 27367
Homologene: 747
Prkdc
Name: protein kinase, DNA activated, catalytic polypeptide
Synonyms: DNA-PKcs, slip, DNAPDcs, XRCC7, DNA-PK, DOXNPH, dxnph
Type: Gene
Species: Mouse
Chromosome: 16
NCBI: 19090
HGNC: HGNC:9413
Homologene: 5037
Camsap3
Name: calmodulin regulated spectrin-associated protein family, member 3
Synonyms: Nezha, 2310057J16Rik
Type: Gene
Species: Mouse
Chromosome: 8
NCBI: 69697
Homologene: 18966
Diaph3
Name: diaphanous related formin 3
Synonyms: p134MDia2, mDia2, 4930417P13Rik, Drf3, Diap3
Type: Gene
Species: Mouse
Chromosome: 14
NCBI: 56419
VEGA: 14
Homologene: 84829
Genetic Alterations
ENU-induced transitions at the following base pair locations (GRCm38):
  • G to A, chromosome 1 at 106,287,103 bp
  • A to G, chromosome 2 at 10,123,413 bp
  • G to A, chromosome 2 at 28,671,886 bp
  • AG to AGCACCACAACTG, chromosome 2 at 130,279,673 bp
  • A to T, chromosome 3 at 60,024,789 bp
  • A to T, chromosome 3 at 83,046,142 bp
  • C to T, chromosome 3 at 89,204,081 bp
  • T to G, chromosome 3 at 138,066,975 bp
  • T to C, chromosome 3 at 141,856,461 bp
  • A to T, chromosome 4 at 58,956,483 bp
  • GACTCTAC to GAC, chromosome 4 at 120,973,085 bp
  • T to C, chromosome 4 at 149,192,596 bp
  • G to A, chromosome 5 at 73,534,614 bp
  • C to T, chromosome 7 at 42,265,859 bp
  • T to C, chromosome 7 at 47,235,106 bp
  • T to A, chromosome 7 at 64,372,486 bp
  • T to A, chromosome 8 at 3,603,941 bp
  • T to C, chromosome 9 at 26,796,532 bp
  • T to C, chromosome 9 at 30,427,743 bp
  • A to G, chromosome 9 at 74,213,361 bp
  • A to T, chromosome 9 at 105,698,691 bp
  • A to C, chromosome 10 at 80,712,158 bp
  • T to A, chromosome 11 at 53,684,235 bp
  • T to C, chromosome 11 at 62,343,302 bp
  • T to A, chromosome 11 at 87,097,177 bp
  • C to A, chromosome 12 at 56,696,863 bp
  • T to A, chromosome 12 at 104,266,517 bp
  • T to C, chromosome 13 at 4,196,512 bp
  • T to A, chromosome 13 at 120,154,813 bp
  • G to T, chromosome 14 at 61,191,248 bp
  • C to T, chromosome 14 at 74,817,085 bp
  • T to C, chromosome 14 at 86,656,538 bp
  • A to G, chromosome 15 at 67,111,346 bp
  • A to T, chromosome 15 at 80,083,345 bp
  • A to G, chromosome 16 at 15,795,075 bp
  • T to C, chromosome 16 at 38,450,309 bp
  • T to C, chromosome 16 at 97,956,772 bp
  • G to A, chromosome 17 at 27,021,319 bp
  • A to T, chromosome 17 at 37,610,313 bp
  • G to A, chromosome 17 at 44,085,264 bp
  • T to C, chromosome 17 at 67,631,865 bp
  • T to C, chromosome 19 at 8,902,632 bp
Phenotype
G1 male heterozygous mice are viable and fertile. The G1 mice were not characterized prior to archiving.
Strain GQC Summary
Gene Specific Genotyping:

To request gene-specific and other genotyping services for a strain, please contact the distribution MMRRC Center for more information.

Background Genetic Quality:

The MMRRC has developed a Genetic Quality Control pipeline using the MiniMUGA array to provide additional information to identify and validate genetic backgrounds of MMRRC strains. For more information on whether genetic background data is available, please contact MMRRC_GeneticQC@med.unc.edu. Note: that MiniMUGA genetic background data is not available on all strains, but can be ordered if desired.

Suggested Control Mice
Littermates of all relevant genotypes.
Donor
Bruce Beutler, M.D., University of Texas Southwestern Medical Center.
Primary Reference
MUTAGENETIX record for R6469 (G1). B. Beutler and colleagues, Center for the Genetics of Host Defense, UT Southwestern, Dallas, TX.
Strain Development
C57BL/6J mice were injected with ENU, resulting in G0 mice that were bred back to C57BL/6J. Sperm was archived from G1 male mice that produced viable G2 mice.


Disclaimer: If MMRRC Strain Genetic Quality Control (GQC; based on MiniMUGA genotyping and analysis) has been completed for this strain, the information might differ from the genetic background information provided by the submitter. MiniMUGA genetic analysis is done on a strain’s tissue samples taken when archived by or ordered from the assigned MMRRC Center.

Colony and Husbandry Information

Colony Surveillance Program and Current Health Reports

Mice recovered from a cryo-archive will have health surveillance performed on recipient females. Health reports will be provided prior to shipment. If you require additional health status information, please email mmrrc@missouri.edu.
Coat Color
Black
MMRRC Breeding System
Random intra-strain mating
Breeding Scheme(s)
When maintaining a live colony, heterozygous mice may be bred together, bred with wild-type siblings, or bred with C57BL/6J inbred mice.
Overall Breeding Performance
Undetermined; during development, mating was done with heterozygous males; viability, fertility and other breeding statistics for heterozygous females and homozygotes are unverified, but presumably would be similar to the C57BL/6J background strain
Average litter size
Undetermined
Recommended wean age
3 weeks

Order Information

Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.

Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.

The donor or their institution limits the distribution to non-profit institutions only.

Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.

Click button to Request this one strain. (Use the MMRRC Catalog Search to request more than one strain.)
MMRRC Item # Description Distribution Fee / Unit (US $)
*Shipping & Handling not included*
Units Notes
044602-MU-RESUS Litter recovered from cryo-archive $5,475.00 / Non-Profit Litter Recovered litter4; additional fees for any special requests.
Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

1 The distribution fee covers the expense of rederiving mice from a live mouse; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

2 An aliquot contains a sufficient number of embryos (in one or more vials or straws and based on the transfer success rate of the MMRRC facility) to transfer into one to three recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.

3 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.

4 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.