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Strain Name:
C57BL/6J-MtgxR6477Btlr/Mmmh
Stock Number:
044609-MU
Citation ID:
RRID:MMRRC_044609-MU
Other Names:
R6477 (G1)
Major Collection:

Strain Information

Hgs
Name: HGF-regulated tyrosine kinase substrate
Synonyms: Hrs, tn
Type: Gene
Species: Mouse
Chromosome: 11
NCBI: 15239
HGNC: HGNC:4897
Homologene: 37954
Mtrr
Name: 5-methyltetrahydrofolate-homocysteine methyltransferase reductase
Type: Gene
Species: Mouse
Chromosome: 13
NCBI: 210009
VEGA: 13
HGNC: HGNC:7473
Homologene: 11419
Pomt1
Name: protein-O-mannosyltransferase 1
Type: Gene
Species: Mouse
Chromosome: 2
NCBI: 99011
HGNC: HGNC:9202
Homologene: 68548
Dst
Name: dystonin
Synonyms: bullous pemphigoid antigen 1, BPAG1-n, BPAG1, Bpag1, Bpag, ah, bullous pemphigoid antigen 1, athetoid, Macf2, 2310001O04Rik, nmf203, A830042E19Rik, nmf339
Type: Gene
Species: Mouse
Chromosome: 1
NCBI: 13518
HGNC: HGNC:1090
Homologene: 136716
Ubald2
Name: UBA-like domain containing 2
Synonyms: D030012E24Rik, 1110014K08Rik, Fam100b
Type: Gene
Species: Mouse
Chromosome: 11
NCBI: 319370
Homologene: 100077
Utp20
Name: UTP20 small subunit processome component
Synonyms: mDRIM, DRIM, 3830408P06Rik
Type: Gene
Species: Mouse
Chromosome: 10
NCBI: 70683
VEGA: 10
Homologene: 38373
Osbpl1a
Name: oxysterol binding protein-like 1A
Synonyms: LOC328902, G430090F17Rik
Type: Gene
Species: Mouse
Chromosome: 18
NCBI: 64291
Homologene: 84746
Genetic Alterations
ENU-induced transitions at the following base pair locations (GRCm38):
  • A to T, chromosome 1 at 23,101,780 bp
  • A to G, chromosome 1 at 34,208,728 bp
  • T to C, chromosome 1 at 135,805,566 bp
  • C to A, chromosome 1 at 163,076,304 bp
  • G to A, chromosome 2 at 32,248,716 bp
  • A to T, chromosome 2 at 69,979,429 bp
  • A to G, chromosome 2 at 87,510,602 bp
  • A to G, chromosome 2 at 87,902,990 bp
  • C to T, chromosome 2 at 132,861,233 bp
  • A to G, chromosome 2 at 165,763,761 bp
  • T to A, chromosome 2 at 167,503,488 bp
  • A to C, chromosome 2 at 174,704,167 bp
  • T to A, chromosome 2 at 180,660,481 bp
  • T to C, chromosome 3 at 20,239,575 bp
  • C to T, chromosome 5 at 67,328,524 bp
  • C to A, chromosome 5 at 75,968,841 bp
  • T to A, chromosome 5 at 120,621,778 bp
  • C to T, chromosome 5 at 144,889,792 bp
  • T to A, chromosome 6 at 24,449,745 bp
  • A to G, chromosome 6 at 83,965,578 bp
  • A to T, chromosome 6 at 124,960,176 bp
  • G to A, chromosome 7 at 27,749,803 bp
  • A to G, chromosome 7 at 34,257,630 bp
  • A to T, chromosome 7 at 46,633,826 bp
  • T to A, chromosome 7 at 83,794,022 bp
  • T to A, chromosome 7 at 102,928,378 bp
  • G to A, chromosome 7 at 104,128,258 bp
  • T to C, chromosome 7 at 104,635,679 bp
  • G to A, chromosome 7 at 105,677,886 bp
  • T to C, chromosome 7 at 120,325,102 bp
  • A to G, chromosome 7 at 126,291,178 bp
  • A to G, chromosome 8 at 3,536,627 bp
  • T to C, chromosome 8 at 75,112,602 bp
  • A to G, chromosome 8 at 81,844,714 bp
  • C to T, chromosome 9 at 65,502,212 bp
  • A to T, chromosome 9 at 80,093,625 bp
  • A to G, chromosome 10 at 88,768,918 bp
  • T to C, chromosome 11 at 36,010,507 bp
  • A to T, chromosome 11 at 56,029,591 bp
  • G to A, chromosome 11 at 72,730,482 bp
  • T to C, chromosome 11 at 116,434,574 bp
  • T to C, chromosome 11 at 120,018,870 bp
  • G to A, chromosome 11 at 120,469,655 bp
  • A to T, chromosome 12 at 76,606,392 bp
  • A to G, chromosome 13 at 9,623,760 bp
  • A to T, chromosome 13 at 68,570,073 bp
  • A to T, chromosome 13 at 74,480,910 bp
  • A to G, chromosome 13 at 100,316,008 bp
  • A to T, chromosome 14 at 54,437,658 bp
  • A to G, chromosome 14 at 56,647,237 bp
  • A to G, chromosome 15 at 73,790,755 bp
  • G to T, chromosome 16 at 42,168,165 bp
  • C to G, chromosome 16 at 45,980,659 bp
  • A to G, chromosome 17 at 24,044,261 bp
  • C to A, chromosome 17 at 32,481,817 bp
  • A to G, chromosome 17 at 34,719,539 bp
  • G to T, chromosome 17 at 59,011,145 bp
  • A to T, chromosome 17 at 88,742,373 bp
  • T to C, chromosome 18 at 12,756,261 bp
  • C to T, chromosome 18 at 35,648,258 bp
  • T to A, chromosome 18 at 42,333,318 bp
  • G to A, chromosome 19 at 10,228,785 bp
Phenotype
G1 male heterozygous mice are viable and fertile. The G1 mice were not characterized prior to archiving.
Strain GQC Summary
Gene Specific Genotyping:

To request gene-specific and other genotyping services for a strain, please contact the distribution MMRRC Center for more information.

Background Genetic Quality:

The MMRRC has developed a Genetic Quality Control pipeline using the MiniMUGA array to provide additional information to identify and validate genetic backgrounds of MMRRC strains. For more information on whether genetic background data is available, please contact MMRRC_GeneticQC@med.unc.edu. Note: that MiniMUGA genetic background data is not available on all strains, but can be ordered if desired.

Suggested Control Mice
Littermates of all relevant genotypes.
Donor
Bruce Beutler, M.D., University of Texas Southwestern Medical Center.
Primary Reference
MUTAGENETIX record for R6477 (G1). B. Beutler and colleagues, Center for the Genetics of Host Defense, UT Southwestern, Dallas, TX.
Strain Development
C57BL/6J mice were injected with ENU, resulting in G0 mice that were bred back to C57BL/6J. Sperm was archived from G1 male mice that produced viable G2 mice.


Disclaimer: If MMRRC Strain Genetic Quality Control (GQC; based on MiniMUGA genotyping and analysis) has been completed for this strain, the information might differ from the genetic background information provided by the submitter. MiniMUGA genetic analysis is done on a strain’s tissue samples taken when archived by or ordered from the assigned MMRRC Center.

Colony and Husbandry Information

Colony Surveillance Program and Current Health Reports

Mice recovered from a cryo-archive will have health surveillance performed on recipient females. Health reports will be provided prior to shipment. If you require additional health status information, please email mmrrc@missouri.edu.
Coat Color
Black
MMRRC Breeding System
Random intra-strain mating
Breeding Scheme(s)
When maintaining a live colony, heterozygous mice may be bred together, bred with wild-type siblings, or bred with C57BL/6J inbred mice.
Overall Breeding Performance
Undetermined; during development, mating was done with heterozygous males; viability, fertility and other breeding statistics for heterozygous females and homozygotes are unverified, but presumably would be similar to the C57BL/6J background strain
Average litter size
Undetermined
Recommended wean age
3 weeks

Order Information

Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.

Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.

The donor or their institution limits the distribution to non-profit institutions only.

Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.

Click button to Request this one strain. (Use the MMRRC Catalog Search to request more than one strain.)
MMRRC Item # Description Distribution Fee / Unit (US $)
*Shipping & Handling not included*
Units Notes
044609-MU-RESUS Litter recovered from cryo-archive $5,475.00 / Non-Profit Litter Recovered litter4; additional fees for any special requests.
Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

1 The distribution fee covers the expense of rederiving mice from a live mouse; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

2 An aliquot contains a sufficient number of embryos (in one or more vials or straws and based on the transfer success rate of the MMRRC facility) to transfer into one to three recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.

3 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.

4 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.