Strain Name:
C57BL/6J-MtgxR6482Btlr/Mmmh
Stock Number:
044614-MU
Citation ID:
RRID:MMRRC_044614-MU
Other Names:
R6482 (G1)
Major Collection:

Strain Information

Wnt5b
Name: wingless-type MMTV integration site family, member 5B
Synonyms: Wnt-5b
Type: Gene
Species: Mouse
Chromosome: 6
NCBI: 22419
Homologene: 22530
Rnf111
Name: ring finger 111
Synonyms: Arkadia
Type: Gene
Species: Mouse
Chromosome: 9
NCBI: 93836
VEGA: 9
Homologene: 9741
Efnb2
Name: ephrin B2
Synonyms: NLERK-1, Epl5, Lerk5, LERK-5, Eplg5, Htk-L, ELF-2
Type: Gene
Species: Mouse
Chromosome: 8
NCBI: 13642
HGNC: HGNC:3227
Homologene: 3019
Usp48
Name: ubiquitin specific peptidase 48
Synonyms: 2810449C13Rik, Usp31, D330022K21Rik
Type: Gene
Species: Mouse
Chromosome: 4
NCBI: 170707
Homologene: 12988
Wapl
Name: WAPL cohesin release factor
Synonyms: Wapal, A530089A20Rik
Type: Gene
Species: Mouse
Chromosome: 14
NCBI: 218914
Homologene: 41002
Akap8l
Name: A kinase anchor protein 8-like
Synonyms: Nakap95
Type: Gene
Species: Mouse
Chromosome: 17
NCBI: 54194
VEGA: 17
Homologene: 8658
Acadvl
Name: acyl-Coenzyme A dehydrogenase, very long chain
Synonyms: VLCAD
Type: Gene
Species: Mouse
Chromosome: 11
NCBI: 11370
HGNC: HGNC:92
Homologene: 5
Plxna4
Name: plexin A4
Synonyms: Plxa4
Type: Gene
Species: Mouse
Chromosome: 6
NCBI: 243743
HGNC: HGNC:9102
Homologene: 77587
Spag9
Name: sperm associated antigen 9
Synonyms: JLP, syd1, 4733401I23Rik, JIP4, Mapk8ip4, 3110018C07Rik, 4831406C20Rik
Type: Gene
Species: Mouse
Chromosome: 11
NCBI: 70834
Homologene: 2954
Dsg2
Name: desmoglein 2
Synonyms: D18Ertd293e
Type: Gene
Species: Mouse
Chromosome: 18
NCBI: 13511
HGNC: HGNC:3049
Homologene: 1464
Casp8ap2
Name: caspase 8 associated protein 2
Synonyms: D4Ertd659e, FLASH
Type: Gene
Species: Mouse
Chromosome: 4
NCBI: 26885
HGNC: HGNC:1510
Homologene: 8066
Obi1
Name: ORC ubiquitin ligase 1
Synonyms: 2610206B13Rik, Rnf219, 2810449K13Rik
Type: Gene
Species: Mouse
Chromosome: 14
NCBI: 72486
VEGA: 14
Homologene: 11578
Zfp142
Name: zinc finger protein 142
Synonyms: 9330177B18Rik
Type: Gene
Species: Mouse
Chromosome: 1
NCBI: 77264
Homologene: 3723
Dpys
Name: dihydropyrimidinase
Synonyms: 1300004I01Rik, 1200017I10Rik, DHPase
Type: Gene
Species: Mouse
Chromosome: 15
NCBI: 64705
HGNC: HGNC:3013
Homologene: 20359
Tmtc1
Name: transmembrane and tetratricopeptide repeat containing 1
Type: Gene
Species: Mouse
Chromosome: 6
NCBI: 387314
Homologene: 65299
Nkx2-2
Name: NK2 homeobox 2
Synonyms: Nkx2.2, Nkx-2.2, tinman
Type: Gene
Species: Mouse
Chromosome: 2
NCBI: 18088
HGNC: HGNC:7835
Homologene: 1879
Klrg1
Name: killer cell lectin-like receptor subfamily G, member 1
Synonyms: 2F1-Ag
Type: Gene
Species: Mouse
Chromosome: 6
NCBI: 50928
HGNC: HGNC:6380
Homologene: 4244
Tarbp1
Name: TAR RNA binding protein 1
Synonyms: Gm17296
Type: Gene
Species: Mouse
Chromosome: 8
NCBI: 212728
Homologene: 38082
Il12rb2
Name: interleukin 12 receptor, beta 2
Synonyms: A930027I18Rik, Ifnm, IL-12RB2
Type: Gene
Species: Mouse
Chromosome: 6
NCBI: 16162
HGNC: HGNC:5972
Homologene: 1197
Zfp385b
Name: zinc finger protein 385B
Synonyms: B830010L13Rik, Zfp533, C130013B13Rik
Type: Gene
Species: Mouse
Chromosome: 2
NCBI: 241494
Homologene: 17606
Mcc
Name: mutated in colorectal cancers
Synonyms: D18Ertd451e
Type: Gene
Species: Mouse
Chromosome: 18
NCBI: 328949
HGNC: HGNC:6935
Homologene: 20539
Ttc21b
Name: tetratricopeptide repeat domain 21B
Synonyms: 2410066K11Rik, aln, line 158, Thm1
Type: Gene
Species: Mouse
Chromosome: 2
NCBI: 73668
Homologene: 57006
Ccdc110
Name: coiled-coil domain containing 110
Synonyms: LOC212392
Type: Gene
Species: Mouse
Chromosome: 8
NCBI: 212392
Homologene: 17668
Vmn1r20
Name: vomeronasal 1 receptor 20
Synonyms: Gm5569
Type: Gene
Species: Mouse
Chromosome: 6
NCBI: 434017
Homologene: 79462
Col22a1
Name: collagen, type XXII, alpha 1
Synonyms: 2310067L16Rik, C80743
Type: Gene
Species: Mouse
Chromosome: 15
NCBI: 69700
Homologene: 43567
Vwde
Name: von Willebrand factor D and EGF domains
Synonyms: LOC232585
Type: Gene
Species: Mouse
Chromosome: 6
NCBI: 232585
Homologene: 35456
Psg21
Name: pregnancy-specific beta-1-glycoprotein 21
Synonyms: cea8, 1600026N13Rik, 1600025N01Rik, 1600019C01Rik
Type: Gene
Species: Mouse
Chromosome: 7
NCBI: 72242
Homologene: 110989
Or8i2
Name: olfactory receptor family 8 subfamily I member 2
Synonyms: MOR207-1, Olfr1104, GA_x6K02T2Q125-48508763-48507833
Type: Gene
Species: Mouse
Chromosome: 2
NCBI: 258763
Homologene: 64916
Itgav
Name: integrin alpha V
Synonyms: CD51, 2610028E01Rik, 1110004F14Rik, alphav-integrin, D430040G12Rik, vitronectin receptor alpha polypeptide (VNRA)
Type: Gene
Species: Mouse
Chromosome: 2
NCBI: 16410
HGNC: HGNC:6150
Homologene: 20510
Fbxo11
Name: F-box protein 11
Synonyms: Jf, GENA 104
Type: Gene
Species: Mouse
Chromosome: 17
NCBI: 225055
Homologene: 11843
Zfp948
Name: zinc finger protein 948
Synonyms: BC049807
Type: Gene
Species: Mouse
Chromosome: 17
NCBI: 381066
VEGA: 17
Hrh1
Name: histamine receptor H1
Synonyms: Hir, Bphs
Type: Gene
Species: Mouse
Chromosome: 6
NCBI: 15465
HGNC: HGNC:5182
Homologene: 668
Plekhg3
Name: pleckstrin homology domain containing, family G (with RhoGef domain) member 3
Synonyms: MGC40768
Type: Gene
Species: Mouse
Chromosome: 12
NCBI: 263406
VEGA: 12
Homologene: 77478
Ano3
Name: anoctamin 3
Synonyms: Tmem16c, B230324K02Rik
Type: Gene
Species: Mouse
Chromosome: 2
NCBI: 228432
Homologene: 57147
Chit1
Name: chitinase 1
Synonyms: 2300002L19Rik
Type: Gene
Species: Mouse
Chromosome: 1
NCBI: 71884
HGNC: HGNC:1936
Homologene: 68318
Pde2a
Name: phosphodiesterase 2A, cGMP-stimulated
Type: Gene
Species: Mouse
Chromosome: 7
NCBI: 207728
HGNC: HGNC:8777
Homologene: 1952
Nppa
Name: natriuretic peptide type A
Synonyms: atrial natriuretic peptide, Pnd, natriuretic peptide precursor A, Anf, ANP
Type: Gene
Species: Mouse
Chromosome: 4
NCBI: 230899
HGNC: HGNC:7939
Homologene: 4498
Pgpep1l
Name: pyroglutamyl-peptidase I-like
Synonyms: C330024D12Rik
Type: Gene
Species: Mouse
Chromosome: 7
NCBI: 78444
Homologene: 25138
Gm35315
Name: predicted gene, 35315
Synonyms: Gm20090
Type: Gene
Species: Mouse
Chromosome: 5
NCBI: 102638847
Homologene: 141161
Eif1ad13
Name: eukaryotic translation initiation factor 1A domain containing 13
Synonyms: Gm21936
Type: Gene
Species: Mouse
Chromosome: 12
NCBI: 100861898
Homologene: 103865
Genetic Alterations
ENU-induced transitions at the following base pair locations (GRCm38):
  • G to A, chromosome 1 at 74,570,217 bp
  • T to C, chromosome 1 at 134,143,242 bp
  • A to G, chromosome 2 at 66,226,900 bp
  • ATCTTCTTCTTCT to ATCTTCTTCTTCTTCT, chromosome 2 at 77,719,648 bp
  • T to C, chromosome 2 at 83,794,270 bp
  • G to C, chromosome 2 at 87,022,525 bp
  • T to A, chromosome 2 at 110,697,055 bp
  • T to A, chromosome 2 at 147,185,976 bp
  • T to C, chromosome 4 at 32,634,813 bp
  • T to A, chromosome 4 at 137,634,921 bp
  • G to A, chromosome 4 at 148,000,871 bp
  • A to T, chromosome 5 at 110,078,089 bp
  • A to G, chromosome 6 at 13,205,844 bp
  • A to G, chromosome 6 at 32,516,737 bp
  • A to G, chromosome 6 at 57,432,108 bp
  • A to G, chromosome 6 at 67,356,686 bp
  • A to G, chromosome 6 at 114,480,763 bp
  • A to T, chromosome 6 at 119,433,612 bp
  • G to T, chromosome 6 at 122,271,453 bp
  • A to G, chromosome 6 at 148,412,745 bp
  • A to T, chromosome 7 at 18,654,739 bp
  • C to T, chromosome 7 at 68,239,067 bp
  • A to G, chromosome 7 at 101,501,037 bp
  • A to T, chromosome 8 at 8,620,637 bp
  • A to T, chromosome 8 at 45,942,788 bp
  • A to G, chromosome 8 at 126,450,695 bp
  • T to C, chromosome 9 at 70,429,607 bp
  • T to C, chromosome 11 at 70,011,562 bp
  • T to C, chromosome 11 at 94,093,502 bp
  • A to G, chromosome 12 at 76,576,004 bp
  • A to G, chromosome 12 at 87,795,795 bp
  • A to G, chromosome 14 at 34,692,692 bp
  • A to T, chromosome 14 at 104,479,817 bp
  • T to C, chromosome 15 at 39,841,973 bp
  • G to A, chromosome 15 at 71,890,489 bp
  • A to G, chromosome 17 at 21,587,551 bp
  • A to G, chromosome 17 at 32,345,396 bp
  • A to T, chromosome 17 at 88,012,658 bp
  • A to T, chromosome 18 at 20,601,314 bp
  • T to C, chromosome 18 at 44,445,864 bp
Phenotype
G1 male heterozygous mice are viable and fertile. The G1 mice were not characterized prior to archiving.
Strain Development
C57BL/6J mice were injected with ENU, resulting in G0 mice that were bred back to C57BL/6J. Sperm was archived from G1 male mice that produced viable G2 mice.
Suggested Control Mice
Wild-type littermates or C57BL/6J mice
MMRRC Genetic QC Summary
The MMRRC Centers have developed a genetic QC pipeline using MiniMUGA array genotyping to provide additional information on strain backgrounds for MMRRC congenic and inbred strains. For more information on when data may be available, or to request genotyping for a strain of interest, please contact mmrrc@missouri.edu. Older strains may not have this information.
Donor
Bruce Beutler, M.D., University of Texas Southwestern Medical Center.
Primary Reference
MUTAGENETIX record for R6482 (G1). B. Beutler and colleagues, Center for the Genetics of Host Defense, UT Southwestern, Dallas, TX.

Colony and Husbandry Information

Colony Surveillance Program and Current Health Reports

Mice recovered from a cryo-archive will have health surveillance performed on recipient females. Health reports will be provided prior to shipment. If you require additional health status information, please email mmrrc@missouri.edu.
Coat Color
Black
MMRRC Breeding System
Random intra-strain mating
Breeding Scheme(s)
When maintaining a live colony, heterozygous mice may be bred together, bred with wild-type siblings, or bred with C57BL/6J inbred mice.
Overall Breeding Performance
Undetermined; during development, mating was done with heterozygous males; viability, fertility and other breeding statistics for heterozygous females and homozygotes are unverified, but presumably would be similar to the C57BL/6J background strain
Average litter size
Undetermined
Recommended wean age
3 weeks

Order Request Information

Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.

Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.

The donor or their institution limits the distribution to non-profit institutions only.

Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.

Click button to Request this one strain. (Use the MMRRC Catalog Search to request more than one strain.)
MMRRC Item # Description Distribution Fee / Unit (US $)
*Shipping & Handling not included*
Units Notes
044614-MU-RESUS Litter recovered from cryo-archive $5,475.00 / Non-Profit Litter Recovered litter4; additional fees for any special requests.
Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

1 The distribution fee covers the expense of rederiving mice from a live mouse; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

2 An aliquot contains a sufficient number of embryos (in one or more vials or straws and based on the transfer success rate of the MMRRC facility) to transfer into one to three recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.

3 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.

4 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.