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Strain Name:
C57BL/6J-MtgxR6494Btlr/Mmmh
Stock Number:
044626-MU
Citation ID:
RRID:MMRRC_044626-MU
Other Names:
R6494 (G1)
Major Collection:

Strain Information

Tsacc
Name: TSSK6 activating co-chaperone
Synonyms: SIP, 1700021C14Rik
Type: Gene
Species: Mouse
Chromosome: 3
NCBI: 76927
Homologene: 16953
Nptn
Name: neuroplastin
Synonyms: Sdfr1, NP65
Type: Gene
Species: Mouse
Chromosome: 9
NCBI: 20320
Homologene: 105617
Kpnb1
Name: karyopherin subunit beta 1
Synonyms: Impnb
Type: Gene
Species: Mouse
Chromosome: 11
NCBI: 16211
HGNC: HGNC:6400
Homologene: 1707
Pkn2
Name: protein kinase N2
Synonyms: PRK2, 6030436C20Rik, Stk7, Prkcl2
Type: Gene
Species: Mouse
Chromosome: 3
NCBI: 109333
HGNC: HGNC:9406
Homologene: 2054
Chic2
Name: cysteine-rich hydrophobic domain 2
Synonyms: BTL, 1700081B18Rik, 4930502K01Rik
Type: Gene
Species: Mouse
Chromosome: 5
NCBI: 74277
HGNC: HGNC:1935
Homologene: 8105
Slc9a8
Name: solute carrier family 9 (sodium/hydrogen exchanger), member 8
Synonyms: NHE8, 1200006P13Rik, 6430709P13Rik
Type: Gene
Species: Mouse
Chromosome: 2
NCBI: 77031
Homologene: 75041
Rbck1
Name: RanBP-type and C3HC4-type zinc finger containing 1
Synonyms: Ubce7ip3, HOIL-1L, HOIL-1
Type: Gene
Species: Mouse
Chromosome: 2
NCBI: 24105
Homologene: 32448
Genetic Alterations
ENU-induced transitions at the following base pair locations (GRCm38):
  • C to A, chromosome 1 at 45,378,327 bp
  • A to T, chromosome 1 at 46,099,431 bp
  • T to A, chromosome 1 at 107,435,346 bp
  • T to A, chromosome 1 at 133,680,448 bp
  • A to T, chromosome 2 at 87,309,176 bp
  • A to T, chromosome 2 at 87,357,632 bp
  • A to G, chromosome 2 at 122,113,225 bp
  • T to C, chromosome 2 at 152,330,966 bp
  • T to A, chromosome 2 at 156,045,470 bp
  • G to A, chromosome 2 at 167,424,291 bp
  • T to A, chromosome 3 at 34,651,097 bp
  • T to C, chromosome 3 at 88,295,396 bp
  • G to A, chromosome 3 at 97,587,167 bp
  • T to A, chromosome 3 at 142,632,008 bp
  • T to C, chromosome 3 at 142,803,668 bp
  • T to A, chromosome 3 at 144,957,298 bp
  • C to T, chromosome 3 at 146,624,577 bp
  • A to G, chromosome 4 at 42,971,924 bp
  • G to T, chromosome 4 at 112,010,712 bp
  • T to A, chromosome 5 at 53,829,383 bp
  • T to C, chromosome 5 at 75,044,282 bp
  • C to T, chromosome 5 at 96,759,564 bp
  • T to C, chromosome 5 at 110,324,722 bp
  • A to G, chromosome 6 at 24,765,746 bp
  • A to T, chromosome 6 at 137,382,642 bp
  • T to A, chromosome 7 at 24,261,357 bp
  • T to C, chromosome 7 at 27,616,349 bp
  • G to T, chromosome 7 at 99,472,899 bp
  • T to C, chromosome 7 at 130,198,550 bp
  • C to T, chromosome 8 at 16,211,695 bp
  • C to T, chromosome 9 at 7,353,479 bp
  • T to G, chromosome 9 at 58,623,752 bp
  • A to T, chromosome 11 at 74,085,192 bp
  • T to C, chromosome 11 at 97,181,648 bp
  • A to T, chromosome 11 at 105,100,019 bp
  • C to T, chromosome 12 at 4,634,792 bp
  • C to T, chromosome 12 at 100,495,407 bp
  • T to A, chromosome 14 at 59,402,414 bp
  • A to T, chromosome 14 at 65,648,222 bp
  • T to A, chromosome 15 at 84,044,322 bp
  • A to G, chromosome 17 at 46,911,081 bp
  • C to A, chromosome 18 at 33,464,305 bp
  • T to A, chromosome 18 at 37,708,541 bp
  • T to A, chromosome 19 at 7,802,286 bp
  • C to T, chromosome 19 at 44,555,305 bp
  • T to A, chromosome 19 at 58,962,794 bp
Phenotype
G1 male heterozygous mice are viable and fertile. The G1 mice were not characterized prior to archiving.
Strain GQC Summary
The MMRRC Centers have developed a Strain GQC pipeline using MiniMUGA array genotyping to provide additional information on strain backgrounds for MMRRC strains. For more information on whether data may be available, or to request genotyping for a strain of interest, please contact MMRRC_GeneticQC@med.unc.edu. Older strains may not have this information available.
Suggested Control Mice
Littermates of all relevant genotypes.
Donor
Bruce Beutler, M.D., University of Texas Southwestern Medical Center.
Primary Reference
MUTAGENETIX record for R6494 (G1). B. Beutler and colleagues, Center for the Genetics of Host Defense, UT Southwestern, Dallas, TX.
Strain Development
C57BL/6J mice were injected with ENU, resulting in G0 mice that were bred back to C57BL/6J. Sperm was archived from G1 male mice that produced viable G2 mice.


Disclaimer: If MMRRC Strain Genetic Quality Control (GQC; based on MiniMUGA genotyping and analysis) has been completed for this strain, the information might differ from the genetic background information provided by the submitter. MiniMUGA genetic analysis is done on a strain’s tissue samples taken when archived by or ordered from the assigned MMRRC Center.

Colony and Husbandry Information

Colony Surveillance Program and Current Health Reports

Mice recovered from a cryo-archive will have health surveillance performed on recipient females. Health reports will be provided prior to shipment. If you require additional health status information, please email mmrrc@missouri.edu.
Coat Color
Black
MMRRC Breeding System
Random intra-strain mating
Breeding Scheme(s)
When maintaining a live colony, heterozygous mice may be bred together, bred with wild-type siblings, or bred with C57BL/6J inbred mice.
Overall Breeding Performance
Undetermined; during development, mating was done with heterozygous males; viability, fertility and other breeding statistics for heterozygous females and homozygotes are unverified, but presumably would be similar to the C57BL/6J background strain
Average litter size
Undetermined
Recommended wean age
3 weeks

Order Request Information

Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.

Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.

The donor or their institution limits the distribution to non-profit institutions only.

Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.

Click button to Request this one strain. (Use the MMRRC Catalog Search to request more than one strain.)
MMRRC Item # Description Distribution Fee / Unit (US $)
*Shipping & Handling not included*
Units Notes
044626-MU-RESUS Litter recovered from cryo-archive $5,475.00 / Non-Profit Litter Recovered litter4; additional fees for any special requests.
Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

1 The distribution fee covers the expense of rederiving mice from a live mouse; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

2 An aliquot contains a sufficient number of embryos (in one or more vials or straws and based on the transfer success rate of the MMRRC facility) to transfer into one to three recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.

3 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.

4 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.