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Strain Name:
C57BL/6J-MtgxR6513Btlr/Mmmh
Stock Number:
044640-MU
Citation ID:
RRID:MMRRC_044640-MU
Other Names:
R6513 (G1)
Major Collection:

Strain Information

Tle4
Name: transducin-like enhancer of split 4
Synonyms: Grg4, ESTM13, ESTM14, Bce1, 5730411M05Rik
Type: Gene
Species: Mouse
Chromosome: 19
NCBI: 21888
VEGA: 19
Homologene: 38259
Shprh
Name: SNF2 histone linker PHD RING helicase
Synonyms: 2610103K11Rik, D230017O13Rik
Type: Gene
Species: Mouse
Chromosome: 10
NCBI: 268281
Homologene: 6489
Lrig2
Name: leucine-rich repeats and immunoglobulin-like domains 2
Synonyms: 4632419I10Rik
Type: Gene
Species: Mouse
Chromosome: 3
NCBI: 269473
Homologene: 8882
Ptf1a
Name: pancreas specific transcription factor, 1a
Synonyms: PTF1-p48, bHLHa29
Type: Gene
Species: Mouse
Chromosome: 2
NCBI: 19213
Homologene: 10304
Gli2
Name: GLI-Kruppel family member GLI2
Type: Gene
Species: Mouse
Chromosome: 1
NCBI: 14633
HGNC: HGNC:4318
Homologene: 12725
Mtap
Name: methylthioadenosine phosphorylase
Synonyms: 1300019I21Rik
Type: Gene
Species: Mouse
Chromosome: 4
NCBI: 66902
HGNC: HGNC:7413
Homologene: 1838
Genetic Alterations
ENU-induced transitions at the following base pair locations (GRCm38):
  • A to G, chromosome 1 at 58,213,053 bp
  • A to G, chromosome 1 at 97,838,027 bp
  • G to A, chromosome 1 at 118,855,554 bp
  • A to T, chromosome 1 at 150,100,128 bp
  • A to G, chromosome 1 at 182,014,355 bp
  • T to A, chromosome 2 at 19,447,037 bp
  • G to A, chromosome 2 at 22,407,332 bp
  • G to A, chromosome 2 at 25,016,538 bp
  • T to A, chromosome 2 at 38,999,958 bp
  • C to G, chromosome 2 at 111,912,883 bp
  • T to A, chromosome 2 at 125,383,671 bp
  • T to C, chromosome 3 at 93,396,112 bp
  • A to T, chromosome 3 at 104,465,729 bp
  • C to T, chromosome 4 at 48,083,255 bp
  • G to A, chromosome 4 at 48,397,534 bp
  • A to G, chromosome 4 at 89,148,261 bp
  • A to T, chromosome 4 at 94,501,403 bp
  • A to T, chromosome 4 at 118,754,027 bp
  • A to C, chromosome 4 at 125,089,904 bp
  • A to G, chromosome 4 at 154,159,405 bp
  • A to G, chromosome 4 at 155,441,286 bp
  • T to A, chromosome 5 at 65,615,601 bp
  • C to A, chromosome 5 at 93,637,532 bp
  • C to T, chromosome 5 at 105,123,120 bp
  • T to C, chromosome 5 at 115,095,936 bp
  • T to A, chromosome 5 at 115,169,134 bp
  • C to A, chromosome 5 at 121,356,196 bp
  • A to G, chromosome 5 at 122,880,239 bp
  • A to G, chromosome 5 at 137,574,531 bp
  • A to G, chromosome 5 at 139,392,818 bp
  • A to G, chromosome 6 at 40,452,619 bp
  • A to G, chromosome 7 at 9,976,597 bp
  • C to A, chromosome 7 at 16,811,112 bp
  • A to G, chromosome 7 at 44,055,745 bp
  • T to C, chromosome 7 at 123,292,156 bp
  • T to C, chromosome 8 at 4,744,507 bp
  • A to G, chromosome 8 at 122,890,180 bp
  • C to T, chromosome 9 at 66,970,890 bp
  • C to T, chromosome 9 at 121,443,756 bp
  • G to A, chromosome 9 at 122,896,105 bp
  • T to C, chromosome 10 at 11,186,893 bp
  • T to A, chromosome 10 at 34,041,634 bp
  • T to C, chromosome 10 at 62,575,034 bp
  • G to A, chromosome 11 at 72,730,482 bp
  • C to T, chromosome 11 at 95,830,687 bp
  • T to A, chromosome 12 at 25,038,435 bp
  • T to C, chromosome 12 at 76,385,830 bp
  • T to A, chromosome 12 at 112,175,492 bp
  • A to T, chromosome 13 at 74,058,463 bp
  • A to T, chromosome 15 at 57,955,382 bp
  • G to A, chromosome 15 at 75,947,468 bp
  • T to C, chromosome 15 at 99,624,275 bp
  • T to G, chromosome 15 at 101,776,752 bp
  • T to C, chromosome 16 at 11,158,765 bp
  • T to A, chromosome 16 at 45,747,877 bp
  • T to C, chromosome 16 at 91,055,766 bp
  • T to C, chromosome 16 at 91,659,947 bp
  • T to C, chromosome 17 at 22,958,741 bp
  • T to C, chromosome 17 at 25,101,412 bp
  • T to A, chromosome 17 at 55,608,093 bp
  • T to A, chromosome 17 at 71,744,706 bp
  • T to C, chromosome 18 at 20,046,238 bp
  • T to G, chromosome 18 at 20,274,630 bp
  • T to A, chromosome 18 at 82,955,710 bp
  • T to C, chromosome 19 at 14,451,692 bp
Phenotype
G1 male heterozygous mice are viable and fertile. The G1 mice were not characterized prior to archiving.
Strain GQC Summary
The MMRRC Centers have developed a Strain GQC pipeline using MiniMUGA array genotyping to provide additional information on strain backgrounds for MMRRC strains. For more information on whether data may be available, or to request genotyping for a strain of interest, please contact MMRRC_GeneticQC@med.unc.edu. Older strains may not have this information available.
Suggested Control Mice
Littermates of all relevant genotypes.
Donor
Bruce Beutler, M.D., University of Texas Southwestern Medical Center.
Primary Reference
MUTAGENETIX record for R6513 (G1). B. Beutler and colleagues, Center for the Genetics of Host Defense, UT Southwestern, Dallas, TX.
Strain Development
C57BL/6J mice were injected with ENU, resulting in G0 mice that were bred back to C57BL/6J. Sperm was archived from G1 male mice that produced viable G2 mice.


Disclaimer: If MMRRC Strain Genetic Quality Control (GQC; based on MiniMUGA genotyping and analysis) has been completed for this strain, the information might differ from the genetic background information provided by the submitter. MiniMUGA genetic analysis is done on a strain’s tissue samples taken when archived by or ordered from the assigned MMRRC Center.

Colony and Husbandry Information

Colony Surveillance Program and Current Health Reports

Mice recovered from a cryo-archive will have health surveillance performed on recipient females. Health reports will be provided prior to shipment. If you require additional health status information, please email mmrrc@missouri.edu.
Coat Color
Black
MMRRC Breeding System
Random intra-strain mating
Breeding Scheme(s)
When maintaining a live colony, heterozygous mice may be bred together, bred with wild-type siblings, or bred with C57BL/6J inbred mice.
Overall Breeding Performance
Undetermined; during development, mating was done with heterozygous males; viability, fertility and other breeding statistics for heterozygous females and homozygotes are unverified, but presumably would be similar to the C57BL/6J background strain
Average litter size
Undetermined
Recommended wean age
3 weeks

Order Request Information

Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.

Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.

The donor or their institution limits the distribution to non-profit institutions only.

Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.

Click button to Request this one strain. (Use the MMRRC Catalog Search to request more than one strain.)
MMRRC Item # Description Distribution Fee / Unit (US $)
*Shipping & Handling not included*
Units Notes
044640-MU-RESUS Litter recovered from cryo-archive $5,475.00 / Non-Profit Litter Recovered litter4; additional fees for any special requests.
Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

1 The distribution fee covers the expense of rederiving mice from a live mouse; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

2 An aliquot contains a sufficient number of embryos (in one or more vials or straws and based on the transfer success rate of the MMRRC facility) to transfer into one to three recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.

3 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.

4 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.