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Strain Name:
C57BL/6J-MtgxR6516Btlr/Mmmh
Stock Number:
044643-MU
Citation ID:
RRID:MMRRC_044643-MU
Other Names:
R6516 (G1)
Major Collection:

Strain Information

Eno2
Name: enolase 2, gamma neuronal
Synonyms: Eno-2, D6Ertd375e, NSE
Type: Gene
Species: Mouse
Chromosome: 6
NCBI: 13807
HGNC: HGNC:3353
Homologene: 74414
Tbx21
Name: T-box 21
Synonyms: Tblym, TBT1, T-bet, Tbet
Type: Gene
Species: Mouse
Chromosome: 11
NCBI: 57765
Homologene: 8353
Plin3
Name: perilipin 3
Synonyms: 1300012C15Rik, Tip47, M6prbp1
Type: Gene
Species: Mouse
Chromosome: 17
NCBI: 66905
VEGA: 17
Homologene: 4247
Slc25a12
Name: solute carrier family 25 (mitochondrial carrier, Aralar), member 12
Synonyms: B230107K20Rik
Type: Gene
Species: Mouse
Chromosome: 2
NCBI: 78830
Homologene: 48235
Adgrl3
Name: adhesion G protein-coupled receptor L3
Synonyms: lectomedin 3, LEC3, D130075K09Rik, 5430402I23Rik, Lphn3
Type: Gene
Species: Mouse
Chromosome: 5
NCBI: 319387
Homologene: 22878
Gpbp1
Name: GC-rich promoter binding protein 1
Synonyms: 1700034P14Rik, D230035M11Rik
Type: Gene
Species: Mouse
Chromosome: 13
NCBI: 73274
Homologene: 11292
Dock4
Name: dedicator of cytokinesis 4
Synonyms: EST N28122, 6330411N01Rik
Type: Gene
Species: Mouse
Chromosome: 12
NCBI: 238130
VEGA: 12
Homologene: 56680
Genetic Alterations
ENU-induced transitions at the following base pair locations (GRCm38):
  • G to T, chromosome 1 at 65,265,781 bp
  • T to C, chromosome 1 at 80,540,461 bp
  • A to T, chromosome 1 at 161,952,666 bp
  • T to A, chromosome 1 at 192,418,781 bp
  • T to C, chromosome 2 at 14,195,980 bp
  • T to C, chromosome 2 at 71,324,083 bp
  • A to G, chromosome 2 at 84,957,761 bp
  • T to C, chromosome 2 at 85,070,727 bp
  • A to T, chromosome 2 at 87,802,770 bp
  • T to C, chromosome 2 at 112,251,217 bp
  • T to A, chromosome 2 at 120,047,950 bp
  • A to G, chromosome 2 at 125,088,107 bp
  • T to A, chromosome 2 at 130,614,301 bp
  • C to T, chromosome 2 at 156,035,199 bp
  • A to G, chromosome 3 at 100,145,676 bp
  • G to A, chromosome 3 at 130,729,067 bp
  • A to C, chromosome 3 at 152,373,416 bp
  • G to A, chromosome 4 at 32,836,427 bp
  • C to T, chromosome 4 at 120,983,106 bp
  • A to T, chromosome 5 at 62,839,264 bp
  • A to G, chromosome 5 at 81,465,272 bp
  • C to T, chromosome 5 at 86,420,128 bp
  • A to T, chromosome 5 at 112,961,549 bp
  • C to T, chromosome 6 at 48,413,272 bp
  • T to G, chromosome 6 at 48,844,068 bp
  • C to A, chromosome 6 at 123,139,406 bp
  • G to T, chromosome 6 at 124,761,709 bp
  • C to G, chromosome 7 at 4,920,202 bp
  • T to A, chromosome 7 at 27,655,987 bp
  • A to T, chromosome 7 at 108,893,765 bp
  • T to C, chromosome 7 at 121,912,112 bp
  • G to A, chromosome 7 at 140,848,805 bp
  • A to G, chromosome 8 at 24,674,687 bp
  • G to C, chromosome 8 at 48,417,222 bp
  • G to T, chromosome 8 at 71,481,780 bp
  • A to C, chromosome 9 at 15,610,614 bp
  • A to G, chromosome 9 at 38,581,472 bp
  • A to T, chromosome 9 at 54,416,676 bp
  • A to G, chromosome 9 at 119,076,203 bp
  • A to G, chromosome 10 at 23,971,666 bp
  • T to A, chromosome 10 at 40,631,745 bp
  • A to G, chromosome 10 at 112,462,000 bp
  • G to A, chromosome 11 at 35,867,302 bp
  • A to G, chromosome 11 at 69,465,386 bp
  • T to C, chromosome 11 at 97,099,956 bp
  • T to A, chromosome 12 at 40,731,899 bp
  • A to T, chromosome 13 at 111,453,102 bp
  • A to T, chromosome 14 at 31,375,672 bp
  • A to T, chromosome 14 at 52,451,129 bp
  • A to G, chromosome 15 at 5,057,081 bp
  • A to G, chromosome 15 at 52,477,804 bp
  • A to T, chromosome 15 at 64,699,387 bp
  • A to G, chromosome 15 at 81,237,868 bp
  • A to G, chromosome 16 at 38,609,404 bp
  • T to A, chromosome 16 at 49,137,633 bp
  • T to C, chromosome 16 at 73,024,353 bp
  • A to T, chromosome 17 at 21,819,373 bp
  • C to T, chromosome 17 at 27,091,370 bp
  • A to T, chromosome 17 at 46,642,255 bp
  • G to A, chromosome 17 at 56,286,223 bp
  • T to C, chromosome 18 at 42,530,892 bp
  • C to A, chromosome 19 at 27,426,008 bp
  • T to A, chromosome 19 at 39,617,429 bp
  • C to T, chromosome 19 at 55,380,958 bp
Phenotype
G1 male heterozygous mice are viable and fertile. The G1 mice were not characterized prior to archiving.
Strain GQC Summary
The MMRRC Centers have developed a Strain GQC pipeline using MiniMUGA array genotyping to provide additional information on strain backgrounds for MMRRC strains. For more information on whether data may be available, or to request genotyping for a strain of interest, please contact MMRRC_GeneticQC@med.unc.edu. Older strains may not have this information available.
Suggested Control Mice
Littermates of all relevant genotypes.
Donor
Bruce Beutler, M.D., University of Texas Southwestern Medical Center.
Primary Reference
MUTAGENETIX record for R6516 (G1). B. Beutler and colleagues, Center for the Genetics of Host Defense, UT Southwestern, Dallas, TX.
Strain Development
C57BL/6J mice were injected with ENU, resulting in G0 mice that were bred back to C57BL/6J. Sperm was archived from G1 male mice that produced viable G2 mice.


Disclaimer: If MMRRC Strain Genetic Quality Control (GQC; based on MiniMUGA genotyping and analysis) has been completed for this strain, the information might differ from the genetic background information provided by the submitter. MiniMUGA genetic analysis is done on a strain’s tissue samples taken when archived by or ordered from the assigned MMRRC Center.

Colony and Husbandry Information

Colony Surveillance Program and Current Health Reports

Mice recovered from a cryo-archive will have health surveillance performed on recipient females. Health reports will be provided prior to shipment. If you require additional health status information, please email mmrrc@missouri.edu.
Coat Color
Black
MMRRC Breeding System
Random intra-strain mating
Breeding Scheme(s)
When maintaining a live colony, heterozygous mice may be bred together, bred with wild-type siblings, or bred with C57BL/6J inbred mice.
Overall Breeding Performance
Undetermined; during development, mating was done with heterozygous males; viability, fertility and other breeding statistics for heterozygous females and homozygotes are unverified, but presumably would be similar to the C57BL/6J background strain
Average litter size
Undetermined
Recommended wean age
3 weeks

Order Request Information

Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.

Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.

The donor or their institution limits the distribution to non-profit institutions only.

Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.

Click button to Request this one strain. (Use the MMRRC Catalog Search to request more than one strain.)
MMRRC Item # Description Distribution Fee / Unit (US $)
*Shipping & Handling not included*
Units Notes
044643-MU-RESUS Litter recovered from cryo-archive $5,475.00 / Non-Profit Litter Recovered litter4; additional fees for any special requests.
Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

1 The distribution fee covers the expense of rederiving mice from a live mouse; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

2 An aliquot contains a sufficient number of embryos (in one or more vials or straws and based on the transfer success rate of the MMRRC facility) to transfer into one to three recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.

3 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.

4 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.