Strain Name:
C57BL/6J-MtgxR6516Btlr/Mmmh
Stock Number:
044643-MU
Citation ID:
RRID:MMRRC_044643-MU
Other Names:
R6516 (G1)
Major Collection:

Strain Information

Eno2
Name: enolase 2, gamma neuronal
Synonyms: NSE, Eno-2, D6Ertd375e
Type: Gene
Species: Mouse
Chromosome: 6
NCBI: 13807
HGNC: HGNC:3353
Homologene: 74414
Tbx21
Name: T-box 21
Synonyms: Tblym, T-bet, TBT1, Tbet
Type: Gene
Species: Mouse
Chromosome: 11
NCBI: 57765
Homologene: 8353
Plin3
Name: perilipin 3
Synonyms: Tip47, M6prbp1, 1300012C15Rik
Type: Gene
Species: Mouse
Chromosome: 17
NCBI: 66905
VEGA: 17
Homologene: 4247
Slc25a12
Name: solute carrier family 25 (mitochondrial carrier, Aralar), member 12
Synonyms: B230107K20Rik
Type: Gene
Species: Mouse
Chromosome: 2
NCBI: 78830
Homologene: 48235
Adgrl3
Name: adhesion G protein-coupled receptor L3
Synonyms: 5430402I23Rik, LEC3, Lphn3, D130075K09Rik, lectomedin 3
Type: Gene
Species: Mouse
Chromosome: 5
NCBI: 319387
Homologene: 22878
Gpbp1
Name: GC-rich promoter binding protein 1
Synonyms: 1700034P14Rik, D230035M11Rik
Type: Gene
Species: Mouse
Chromosome: 13
NCBI: 73274
Homologene: 11292
Dock4
Name: dedicator of cytokinesis 4
Synonyms: EST N28122, 6330411N01Rik
Type: Gene
Species: Mouse
Chromosome: 12
NCBI: 238130
VEGA: 12
Homologene: 56680
Wdr3
Name: WD repeat domain 3
Synonyms: D030020G18Rik
Type: Gene
Species: Mouse
Chromosome: 3
NCBI: 269470
Homologene: 4937
Vti1a
Name: vesicle transport through interaction with t-SNAREs 1A
Synonyms: 1110014F16Rik, Vti1-rp2, 4921537J05Rik, 1110018K19Rik
Type: Gene
Species: Mouse
Chromosome: 19
NCBI: 53611
VEGA: 19
Homologene: 39963
Wwc1
Name: WW, C2 and coiled-coil domain containing 1
Synonyms: Kibra
Type: Gene
Species: Mouse
Chromosome: 11
NCBI: 211652
Homologene: 69180
Pikfyve
Name: phosphoinositide kinase, FYVE type zinc finger containing
Synonyms: PipkIII, Pip5k3, 5230400C17Rik
Type: Gene
Species: Mouse
Chromosome: 1
NCBI: 18711
Homologene: 32115
Dmxl2
Name: Dmx-like 2
Synonyms: 6430411K14Rik, E130119P06Rik
Type: Gene
Species: Mouse
Chromosome: 9
NCBI: 235380
HGNC: HGNC:2938
Homologene: 41022
Robo1
Name: roundabout guidance receptor 1
Synonyms: DUTT1
Type: Gene
Species: Mouse
Chromosome: 16
NCBI: 19876
Homologene: 2206
Adam18
Name: a disintegrin and metallopeptidase domain 18
Synonyms: Dtgn3, Adam27
Type: Gene
Species: Mouse
Chromosome: 8
NCBI: 13524
HGNC: HGNC:196
Homologene: 74941
Pum3
Name: pumilio RNA-binding family member 3
Synonyms: 1110069H02Rik, D19Bwg1357e
Type: Gene
Species: Mouse
Chromosome: 19
NCBI: 52874
VEGA: 19
Homologene: 5762
Tenm3
Name: teneurin transmembrane protein 3
Synonyms: Odz3, Ten-m3, 2610100B16Rik
Type: Gene
Species: Mouse
Chromosome: 8
NCBI: 23965
Homologene: 22673
Smap2
Name: small ArfGAP 2
Synonyms: Smap2, 1810031K02Rik, Smap1l
Type: Gene
Species: Mouse
Chromosome: 4
NCBI: 69780
Homologene: 41489
Tcerg1
Name: transcription elongation regulator 1 (CA150)
Synonyms: 2410022J09Rik, p144, Taf2s, Fbp28, 2900090C16Rik, ca150
Type: Gene
Species: Mouse
Chromosome: 18
NCBI: 56070
Homologene: 4879
Usp33
Name: ubiquitin specific peptidase 33
Synonyms: 9830169D19Rik, Vdu1
Type: Gene
Species: Mouse
Chromosome: 3
NCBI: 170822
Homologene: 8996
Tnks1bp1
Name: tankyrase 1 binding protein 1
Synonyms: TAB182
Type: Gene
Species: Mouse
Chromosome: 2
NCBI: 228140
Homologene: 14117
Clec4a2
Name: C-type lectin domain family 4, member a2
Synonyms: Dcir1, DCIR, Clecsf6, dendritic cell immunoreceptor
Type: Gene
Species: Mouse
Chromosome: 6
NCBI: 26888
Homologene: 49291
Itpr3
Name: inositol 1,4,5-triphosphate receptor 3
Synonyms: Ip3r3, Itpr-3, tf
Type: Gene
Species: Mouse
Chromosome: 17
NCBI: 16440
HGNC: HGNC:6182
Homologene: 1675
Rpl34
Name: ribosomal protein L34
Synonyms: 1100001I22Rik
Type: Gene
Species: Mouse
Chromosome: 3
NCBI: 68436
Homologene: 105146
Dnah2
Name: dynein, axonemal, heavy chain 2
Synonyms: 2900022L05Rik, Dnhd3, Dnahc2, D330014H01Rik
Type: Gene
Species: Mouse
Chromosome: 11
NCBI: 327954
HGNC: HGNC:2948
Homologene: 72110
Myh15
Name: myosin, heavy chain 15
Synonyms: EG667772
Type: Gene
Species: Mouse
Chromosome: 16
NCBI: 667772
VEGA: 16
Homologene: 18929
C7
Name: complement component 7
Synonyms: LOC383055
Type: Gene
Species: Mouse
Chromosome: 15
NCBI: 109828
VEGA: 15
HGNC: HGNC:1346
Homologene: 489
Dock10
Name: dedicator of cytokinesis 10
Synonyms: Jr4, Zizimin3, ZIZ3, Jr5, A630054M16Rik, 9330153B10Rik
Type: Gene
Species: Mouse
Chromosome: 1
NCBI: 210293
Homologene: 45952
Sptbn5
Name: spectrin beta, non-erythrocytic 5
Synonyms: Spnb5, EG640524
Type: Gene
Species: Mouse
Chromosome: 2
NCBI: 640524
Homologene: 41150
Fer1l4
Name: fer-1 like family member 4
Synonyms: 9130402C12Rik
Type: Gene
Species: Mouse
Chromosome: 2
NCBI: 74562
Homologene: 19075
Klc4
Name: kinesin light chain 4
Synonyms: Knsl8, 1200014P03Rik
Type: Gene
Species: Mouse
Chromosome: 17
NCBI: 74764
VEGA: 17
Homologene: 12584
Deup1
Name: deuterosome assembly protein 1
Synonyms: Ccdc67, 4933401K09Rik
Type: Gene
Species: Mouse
Chromosome: 9
NCBI: 234964
Homologene: 18760
Adcy8
Name: adenylate cyclase 8
Synonyms: AC8
Type: Gene
Species: Mouse
Chromosome: 15
NCBI: 11514
VEGA: 15
HGNC: HGNC:239
Homologene: 37443
Cimap1a
Name: ciliary microtubule associated protein 1A
Synonyms: 1700011O04Rik, Odf3, SHIPPO1
Type: Gene
Species: Mouse
Chromosome: 7
NCBI: 69287
Homologene: 12306
Krba1
Name: KRAB-A domain containing 1
Synonyms: A930040G15Rik
Type: Gene
Species: Mouse
Chromosome: 6
NCBI: 77827
Homologene: 15880
Dthd1
Name: death domain containing 1
Synonyms: Gm17384
Type: Gene
Species: Mouse
Chromosome: 5
NCBI: 100322896
Homologene: 123510
Zfp628
Name: zinc finger protein 628
Synonyms: Zec
Type: Gene
Species: Mouse
Chromosome: 7
NCBI: 232816
Homologene: 72200
Kcnc2
Name: potassium voltage gated channel, Shaw-related subfamily, member 2
Synonyms: Kv3.2, KShIIIA
Type: Gene
Species: Mouse
Chromosome: 10
NCBI: 268345
VEGA: 10
HGNC: HGNC:6234
Homologene: 71199
Taar5
Name: trace amine-associated receptor 5
Synonyms: LOC215854
Type: Gene
Species: Mouse
Chromosome: 10
NCBI: 215854
Homologene: 20850
Grk3
Name: G protein-coupled receptor kinase 3
Synonyms: Adrbk-2, 4833444A01Rik, beta ARK2, Adrbk2, Bark-2
Type: Gene
Species: Mouse
Chromosome: 5
NCBI: 320129
HGNC: HGNC:290
Homologene: 21072
Zfp820
Name: zinc finger protein 820
Synonyms: 2610036F08Rik
Type: Gene
Species: Mouse
Chromosome: 17
NCBI: 75424
VEGA: 17
Tmem236
Name: transmembrane protein 236
Synonyms: 2010003H20Rik, Fam23a
Type: Gene
Species: Mouse
Chromosome: 2
NCBI: 625286
Homologene: 46598
Kcnh1
Name: potassium voltage-gated channel, subfamily H (eag-related), member 1
Synonyms: Kv10.1, Eag1, ether a go-go
Type: Gene
Species: Mouse
Chromosome: 1
NCBI: 16510
HGNC: HGNC:6250
Homologene: 68242
Or4e2
Name: olfactory receptor family 4 subfamily E member 2
Synonyms: Olfr1509, MOR83, MOR244-3, GA_x6K02T2RJGY-534312-533386
Type: Gene
Species: Mouse
Chromosome: 14
NCBI: 57271
HGNC: HGNC:8297
Homologene: 41378
Ano8
Name: anoctamin 8
Synonyms: Tmem16h
Type: Gene
Species: Mouse
Chromosome: 8
NCBI: 382014
Homologene: 124473
Scnn1b
Name: sodium channel, nonvoltage-gated 1 beta
Synonyms: ENaC beta
Type: Gene
Species: Mouse
Chromosome: 7
NCBI: 20277
Homologene: 133555
Arhgap31
Name: Rho GTPase activating protein 31
Synonyms: CdGAP
Type: Gene
Species: Mouse
Chromosome: 16
NCBI: 12549
Homologene: 10644
Slc43a3
Name: solute carrier family 43, member 3
Synonyms: Eeg1, SEEEG-1
Type: Gene
Species: Mouse
Chromosome: 2
NCBI: 58207
Homologene: 40925
Or5w18
Name: olfactory receptor family 5 subfamily W member 18
Synonyms: MOR177-14, Olfr1143, GA_x6K02T2Q125-49303473-49304405
Type: Gene
Species: Mouse
Chromosome: 2
NCBI: 258290
Homologene: 74079
Slc24a5
Name: solute carrier family 24, member 5
Synonyms: F630045L20Rik, NCX5, Oca6, NCKX5
Type: Gene
Species: Mouse
Chromosome: 2
NCBI: 317750
Homologene: 18400
Ankrd6
Name: ankyrin repeat domain 6
Synonyms: diversin
Type: Gene
Species: Mouse
Chromosome: 4
NCBI: 140577
Homologene: 8967
Plcd1
Name: phospholipase C, delta 1
Synonyms: PLC-delta 1
Type: Gene
Species: Mouse
Chromosome: 9
NCBI: 18799
VEGA: 9
HGNC: HGNC:9060
Homologene: 21252
Mchr1
Name: melanin-concentrating hormone receptor 1
Synonyms: Gpr24, melanin-concentrating hormone receptor 1, MCH-1R, Gpr24-9, Mch1r
Type: Gene
Species: Mouse
Chromosome: 15
NCBI: 207911
VEGA: 15
HGNC: HGNC:4479
Homologene: 21097
Fastkd5
Name: FAST kinase domains 5
Type: Gene
Species: Mouse
Chromosome: 2
NCBI: 380601
Homologene: 36400
Cyp2c67
Name: cytochrome P450, family 2, subfamily c, polypeptide 67
Synonyms: C730004C24Rik
Type: Gene
Species: Mouse
Chromosome: 19
NCBI: 545288
Homologene: 74936
Or8b48
Name: olfactory receptor family 8 subfamily B member 48
Synonyms: Olfr912, GA_x6K02T2PVTD-32283590-32284522, MOR165-4
Type: Gene
Species: Mouse
Chromosome: 9
NCBI: 258806
VEGA: 9
Homologene: 115510
Nutm1
Name: NUT midline carcinoma, family member 1
Synonyms: BC125332, Nut
Type: Gene
Species: Mouse
Chromosome: 2
NCBI: 213765
Homologene: 18185
Or10a3n
Name: olfactory receptor family 10 subfamily A member 3N
Synonyms: Olfr519, MOR268-6, GA_x6K02T2PBJ9-11224559-11223615
Type: Gene
Species: Mouse
Chromosome: 7
NCBI: 277935
Homologene: 17186
Tmprss11a
Name: transmembrane protease, serine 11a
Synonyms: LOC194597
Type: Gene
Species: Mouse
Chromosome: 5
NCBI: 194597
Homologene: 62723
Ddo
Name: D-aspartate oxidase
Synonyms: 5330420D20Rik, 5730402C02Rik
Type: Gene
Species: Mouse
Chromosome: 10
NCBI: 70503
VEGA: 10
HGNC: HGNC:2727
Homologene: 101531
Sh3bp5
Name: SH3-domain binding protein 5 (BTK-associated)
Synonyms: Sab
Type: Gene
Species: Mouse
Chromosome: 14
NCBI: 24056
Homologene: 23450
Ttc9b
Name: tetratricopeptide repeat domain 9B
Synonyms: 2900074C18Rik
Type: Gene
Species: Mouse
Chromosome: 7
NCBI: 73032
Homologene: 19938
Rpl15-ps6
Name: ribosomal protein L15, pseudogene 6
Synonyms: Gm10020
Type: Gene
Species: Mouse
Chromosome: 15
NCBI: 100039748
VEGA: 15
4930558K02Rik
Name: RIKEN cDNA 4930558K02 gene
Type: Gene
Species: Mouse
Chromosome: 1
NCBI: 75368
Homologene: 51403
Tmem176a
Name: transmembrane protein 176A
Synonyms: 0610011I04Rik
Type: Gene
Species: Mouse
Chromosome: 6
NCBI: 66058
Homologene: 10224
Genetic Alterations
ENU-induced transitions at the following base pair locations (GRCm38):
  • G to T, chromosome 1 at 65,265,781 bp
  • T to C, chromosome 1 at 80,540,461 bp
  • A to T, chromosome 1 at 161,952,666 bp
  • T to A, chromosome 1 at 192,418,781 bp
  • T to C, chromosome 2 at 14,195,980 bp
  • T to C, chromosome 2 at 71,324,083 bp
  • A to G, chromosome 2 at 84,957,761 bp
  • T to C, chromosome 2 at 85,070,727 bp
  • A to T, chromosome 2 at 87,802,770 bp
  • T to C, chromosome 2 at 112,251,217 bp
  • T to A, chromosome 2 at 120,047,950 bp
  • A to G, chromosome 2 at 125,088,107 bp
  • T to A, chromosome 2 at 130,614,301 bp
  • C to T, chromosome 2 at 156,035,199 bp
  • A to G, chromosome 3 at 100,145,676 bp
  • G to A, chromosome 3 at 130,729,067 bp
  • A to C, chromosome 3 at 152,373,416 bp
  • G to A, chromosome 4 at 32,836,427 bp
  • C to T, chromosome 4 at 120,983,106 bp
  • A to T, chromosome 5 at 62,839,264 bp
  • A to G, chromosome 5 at 81,465,272 bp
  • C to T, chromosome 5 at 86,420,128 bp
  • A to T, chromosome 5 at 112,961,549 bp
  • C to T, chromosome 6 at 48,413,272 bp
  • T to G, chromosome 6 at 48,844,068 bp
  • C to A, chromosome 6 at 123,139,406 bp
  • G to T, chromosome 6 at 124,761,709 bp
  • C to G, chromosome 7 at 4,920,202 bp
  • T to A, chromosome 7 at 27,655,987 bp
  • A to T, chromosome 7 at 108,893,765 bp
  • T to C, chromosome 7 at 121,912,112 bp
  • G to A, chromosome 7 at 140,848,805 bp
  • A to G, chromosome 8 at 24,674,687 bp
  • G to C, chromosome 8 at 48,417,222 bp
  • G to T, chromosome 8 at 71,481,780 bp
  • A to C, chromosome 9 at 15,610,614 bp
  • A to G, chromosome 9 at 38,581,472 bp
  • A to T, chromosome 9 at 54,416,676 bp
  • A to G, chromosome 9 at 119,076,203 bp
  • A to G, chromosome 10 at 23,971,666 bp
  • T to A, chromosome 10 at 40,631,745 bp
  • A to G, chromosome 10 at 112,462,000 bp
  • G to A, chromosome 11 at 35,867,302 bp
  • A to G, chromosome 11 at 69,465,386 bp
  • T to C, chromosome 11 at 97,099,956 bp
  • T to A, chromosome 12 at 40,731,899 bp
  • A to T, chromosome 13 at 111,453,102 bp
  • A to T, chromosome 14 at 31,375,672 bp
  • A to T, chromosome 14 at 52,451,129 bp
  • A to G, chromosome 15 at 5,057,081 bp
  • A to G, chromosome 15 at 52,477,804 bp
  • A to T, chromosome 15 at 64,699,387 bp
  • A to G, chromosome 15 at 81,237,868 bp
  • A to G, chromosome 16 at 38,609,404 bp
  • T to A, chromosome 16 at 49,137,633 bp
  • T to C, chromosome 16 at 73,024,353 bp
  • A to T, chromosome 17 at 21,819,373 bp
  • C to T, chromosome 17 at 27,091,370 bp
  • A to T, chromosome 17 at 46,642,255 bp
  • G to A, chromosome 17 at 56,286,223 bp
  • T to C, chromosome 18 at 42,530,892 bp
  • C to A, chromosome 19 at 27,426,008 bp
  • T to A, chromosome 19 at 39,617,429 bp
  • C to T, chromosome 19 at 55,380,958 bp
Phenotype
G1 male heterozygous mice are viable and fertile. The G1 mice were not characterized prior to archiving.
Strain Development
C57BL/6J mice were injected with ENU, resulting in G0 mice that were bred back to C57BL/6J. Sperm was archived from G1 male mice that produced viable G2 mice.
Suggested Control Mice
Wild-type littermates or C57BL/6J mice
MMRRC Genetic QC Summary
The MMRRC Centers have developed a genetic QC pipeline using MiniMUGA array genotyping to provide additional information on strain backgrounds for MMRRC congenic and inbred strains. For more information on when data may be available, or to request genotyping for a strain of interest, please contact mmrrc@missouri.edu. Older strains may not have this information.
Donor
Bruce Beutler, M.D., University of Texas Southwestern Medical Center.
Primary Reference
MUTAGENETIX record for R6516 (G1). B. Beutler and colleagues, Center for the Genetics of Host Defense, UT Southwestern, Dallas, TX.

Colony and Husbandry Information

Colony Surveillance Program and Current Health Reports

Mice recovered from a cryo-archive will have health surveillance performed on recipient females. Health reports will be provided prior to shipment. If you require additional health status information, please email mmrrc@missouri.edu.
Coat Color
Black
MMRRC Breeding System
Random intra-strain mating
Breeding Scheme(s)
When maintaining a live colony, heterozygous mice may be bred together, bred with wild-type siblings, or bred with C57BL/6J inbred mice.
Overall Breeding Performance
Undetermined; during development, mating was done with heterozygous males; viability, fertility and other breeding statistics for heterozygous females and homozygotes are unverified, but presumably would be similar to the C57BL/6J background strain
Average litter size
Undetermined
Recommended wean age
3 weeks

Order Request Information

Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.

Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.

The donor or their institution limits the distribution to non-profit institutions only.

Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.

Click button to Request this one strain. (Use the MMRRC Catalog Search to request more than one strain.)
MMRRC Item # Description Distribution Fee / Unit (US $)
*Shipping & Handling not included*
Units Notes
044643-MU-RESUS Litter recovered from cryo-archive $5,475.00 / Non-Profit Litter Recovered litter4; additional fees for any special requests.
Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

1 The distribution fee covers the expense of rederiving mice from a live mouse; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

2 An aliquot contains a sufficient number of embryos (in one or more vials or straws and based on the transfer success rate of the MMRRC facility) to transfer into one to three recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.

3 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.

4 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.