Strain Name:
C57BL/6J-MtgxR6518Btlr/Mmmh
Stock Number:
044645-MU
Citation ID:
RRID:MMRRC_044645-MU
Other Names:
R6518 (G1)
Major Collection:

Strain Information

Gtf2h3
Name: general transcription factor IIH, polypeptide 3
Synonyms: 5033417D07Rik, BTF2, D5Ertd679e, 34kDa
Type: Gene
Species: Mouse
Chromosome: 5
NCBI: 209357
HGNC: HGNC:4657
Homologene: 1160
Mindy3
Name: MINDY lysine 48 deubiquitinase 3
Synonyms: 1810041E18Rik, 5830410F13Rik, 2310047O13Rik, Fam188a
Type: Gene
Species: Mouse
Chromosome: 2
NCBI: 66960
Homologene: 11478
Cdkn1b
Name: cyclin dependent kinase inhibitor 1B
Synonyms: p27Kip1, p27
Type: Gene
Species: Mouse
Chromosome: 6
NCBI: 12576
HGNC: HGNC:1785
Homologene: 2999
Gpr139
Name: G protein-coupled receptor 139
Synonyms: LOC209776, GPRg1
Type: Gene
Species: Mouse
Chromosome: 7
NCBI: 209776
Homologene: 45860
Dcaf1
Name: DDB1 and CUL4 associated factor 1
Synonyms: B930007L02Rik, Vprbp
Type: Gene
Species: Mouse
Chromosome: 9
NCBI: 321006
Homologene: 8805
Wdr70
Name: WD repeat domain 70
Synonyms: 4833422F06Rik
Type: Gene
Species: Mouse
Chromosome: 15
NCBI: 545085
VEGA: 15
Homologene: 9972
Ddx4
Name: DEAD box helicase 4
Synonyms: mvh / m'vasa, VASA, Mvh, DEAD (Asp-Glu-Ala-Asp) box polypeptide 4
Type: Gene
Species: Mouse
Chromosome: 13
NCBI: 13206
VEGA: 13
Homologene: 49227
Pias1
Name: protein inhibitor of activated STAT 1
Synonyms: 2900068C24Rik, GBP, Ddxbp1
Type: Gene
Species: Mouse
Chromosome: 9
NCBI: 56469
VEGA: 9
HGNC: HGNC:2752
Homologene: 22953
Lmo1
Name: LIM domain only 1
Synonyms: Ttg1, Rbtn1, Rbtn-1
Type: Gene
Species: Mouse
Chromosome: 7
NCBI: 109594
HGNC: HGNC:6641
Homologene: 48101
Seh1l
Name: SEH1-like (S. cerevisiae
Synonyms: 2610007A16Rik
Type: Gene
Species: Mouse
Chromosome: 18
NCBI: 72124
VEGA: 18
Homologene: 68701
Angptl6
Name: angiopoietin-like 6
Synonyms: Arp5, AGF, Angiopoietin-related growth factor, 6330404E11Rik
Type: Gene
Species: Mouse
Chromosome: 9
NCBI: 70726
Homologene: 88931
Timm23
Name: translocase of inner mitochondrial membrane 23
Synonyms: Tim23
Type: Gene
Species: Mouse
Chromosome: 14
NCBI: 53600
Homologene: 9651
Obi1
Name: ORC ubiquitin ligase 1
Synonyms: 2810449K13Rik, 2610206B13Rik, Rnf219
Type: Gene
Species: Mouse
Chromosome: 14
NCBI: 72486
VEGA: 14
Homologene: 11578
Ckap2
Name: cytoskeleton associated protein 2
Synonyms: LB1
Type: Gene
Species: Mouse
Chromosome: 8
NCBI: 80986
HGNC: HGNC:1990
Homologene: 10070
Ice1
Name: interactor of little elongation complex ELL subunit 1
Synonyms: BC018507
Type: Gene
Species: Mouse
Chromosome: 13
NCBI: 218333
VEGA: 13
Homologene: 18902
Asxl3
Name: ASXL transcriptional regulator 3
Synonyms: LOC381127, D930044O18Rik, D430002O22Rik, C230079D11Rik
Type: Gene
Species: Mouse
Chromosome: 18
NCBI: 211961
Homologene: 19371
Dsg3
Name: desmoglein 3
Type: Gene
Species: Mouse
Chromosome: 18
NCBI: 13512
VEGA: 18
HGNC: HGNC:3050
Homologene: 55513
Tmem86b
Name: transmembrane protein 86B
Synonyms: C330014O21Rik
Type: Gene
Species: Mouse
Chromosome: 7
NCBI: 68255
Homologene: 52159
Dnah10
Name: dynein, axonemal, heavy chain 10
Synonyms: Dnahc10
Type: Gene
Species: Mouse
Chromosome: 5
NCBI: 56087
HGNC: HGNC:2941
Homologene: 25816
Piezo2
Name: piezo-type mechanosensitive ion channel component 2
Synonyms: 9430028L06Rik, 9030411M15Rik, Fam38b2, Piezo2, Fam38b
Type: Gene
Species: Mouse
Chromosome: 18
NCBI: 667742
Homologene: 49695
Col7a1
Name: collagen, type VII, alpha 1
Type: Gene
Species: Mouse
Chromosome: 9
NCBI: 12836
HGNC: HGNC:2214
Homologene: 73
Mcc
Name: mutated in colorectal cancers
Synonyms: D18Ertd451e
Type: Gene
Species: Mouse
Chromosome: 18
NCBI: 328949
HGNC: HGNC:6935
Homologene: 20539
Igkv6-29
Name: immunoglobulin kappa chain variable 6-29
Synonyms: Gm16692
Type: Gene
Species: Mouse
Chromosome: 6
NCBI: 620191
HGNC: HGNC:5834
Abcc12
Name: ATP-binding cassette, sub-family C member 12
Synonyms: MRP9, 4930467B22Rik
Type: Gene
Species: Mouse
Chromosome: 8
NCBI: 244562
Homologene: 57211
Ormdl1
Name: ORM1-like 1 (S. cerevisiae)
Synonyms: C730042F17Rik
Type: Gene
Species: Mouse
Chromosome: 1
NCBI: 227102
Homologene: 41135
C4b
Name: complement C4B (Chido blood group)
Synonyms: Ss, C4
Type: Gene
Species: Mouse
Chromosome: 17
NCBI: 12268
Homologene: 36030
Vmn2r85
Name: vomeronasal 2, receptor 85
Synonyms: EG623734
Type: Gene
Species: Mouse
Chromosome: 10
NCBI: 623734
Homologene: 129606
Cog2
Name: component of oligomeric golgi complex 2
Synonyms: 1190002B08Rik, 2700012E02Rik, Cog2
Type: Gene
Species: Mouse
Chromosome: 8
NCBI: 76332
HGNC: HGNC:6546
Homologene: 7206
Pdzrn3
Name: PDZ domain containing RING finger 3
Synonyms: semaphorin cytoplasmic domain-associated protein 3A, 1110020C07Rik, LNX3, Semcap3
Type: Gene
Species: Mouse
Chromosome: 6
NCBI: 55983
Homologene: 10328
Rsph10b
Name: radial spoke head 10 homolog B (Chlamydomonas)
Synonyms: 4930526H21Rik, Rsph10b2
Type: Gene
Species: Mouse
Chromosome: 5
Homologene: 18321
C2
Name: complement C2
Synonyms: classical-complement pathway C3/C5 convertase
Type: Gene
Species: Mouse
Chromosome: 17
NCBI: 12263
HGNC: HGNC:1248
Homologene: 45
Sp140
Name: Sp140 nuclear body protein
Type: Gene
Species: Mouse
Chromosome: 1
NCBI: 434484
Homologene: 128391
C1ra
Name: complement component 1, r subcomponent A
Synonyms: mC1rA
Type: Gene
Species: Mouse
Chromosome: 6
NCBI: 50909
HGNC: HGNC:1246
Homologene: 1313
Prkd1
Name: protein kinase D1
Synonyms: Pkcm, PKD1, Prkcm
Type: Gene
Species: Mouse
Chromosome: 12
NCBI: 18760
VEGA: 12
HGNC: HGNC:9407
Homologene: 55680
Or11a4
Name: olfactory receptor family 11 subfamily A member 4
Synonyms: MOR121-1, GA_x6K02T2PSCP-1665046-1665987, Olfr96
Type: Gene
Species: Mouse
Chromosome: 17
NCBI: 258507
HGNC: HGNC:8176
Homologene: 27189
Samhd1
Name: SAM domain and HD domain, 1
Synonyms: E330031J07Rik
Type: Gene
Species: Mouse
Chromosome: 2
NCBI: 56045
Homologene: 9160
4921539E11Rik
Name: RIKEN cDNA 4921539E11 gene
Type: Gene
Species: Mouse
Chromosome: 4
NCBI: 70941
Homologene: 52843
Wars2
Name: tryptophanyl tRNA synthetase 2 (mitochondrial)
Synonyms: 9430020O07Rik, TrpRS
Type: Gene
Species: Mouse
Chromosome: 3
NCBI: 70560
Homologene: 5673
Vmn2r71
Name: vomeronasal 2, receptor 71
Synonyms: EG233445
Type: Gene
Species: Mouse
Chromosome: 7
NCBI: 233445
Homologene: 115466
Or4e5
Name: olfactory receptor family 4 subfamily E member 5
Synonyms: GA_x6K02T2RJGY-491851-492792, MOR244-1, MOR28, Olfr1507
Type: Gene
Species: Mouse
Chromosome: 14
NCBI: 57269
HGNC: HGNC:8296
Homologene: 10736
Or13j1
Name: olfactory receptor family 13 subfamily J member 1
Synonyms: mOR17, MOR262-4, GA_x6K02T2N78B-16230286-16231224, Olfr71
Type: Gene
Species: Mouse
Chromosome: 4
NCBI: 56015
Homologene: 10460
Zfp677
Name: zinc finger protein 677
Synonyms: A830058L05Rik
Type: Gene
Species: Mouse
Chromosome: 17
NCBI: 210503
Homologene: 138402
Atp13a2
Name: ATPase type 13A2
Synonyms: 1110012E06Rik
Type: Gene
Species: Mouse
Chromosome: 4
NCBI: 74772
Homologene: 56940
Cenpn
Name: centromere protein N
Synonyms: 2610510J17Rik
Type: Gene
Species: Mouse
Chromosome: 8
NCBI: 72155
Homologene: 12450
Lipt1
Name: lipoyltransferase 1
Synonyms: EG623661
Type: Gene
Species: Mouse
Chromosome: 1
NCBI: 623661
Homologene: 50569
Tmem17
Name: transmembrane protein 17
Type: Gene
Species: Mouse
Chromosome: 11
NCBI: 103765
Homologene: 17795
Speer3
Name: spermatogenesis associated glutamate (E)-rich protein 3
Synonyms: SPEER-3, 4933405P08Rik
Type: Gene
Species: Mouse
Chromosome: 5
NCBI: 71026
Homologene: 69402
Try5
Name: trypsin 5
Synonyms: Tc, 1810049H19Rik
Type: Gene
Species: Mouse
Chromosome: 6
NCBI: 103964
Homologene: 106639
Gm16686
Name: predicted gene, 16686
Type: Gene
Species: Mouse
Chromosome: 4
Spata31f1e
Name: spermatogenesis associated 31 subfamily F member 1E
Synonyms: Gm12394
Type: Gene
Species: Mouse
Chromosome: 4
Smim24
Name: small integral membrane protein 24
Synonyms: 2210404O07Rik
Type: Gene
Species: Mouse
Chromosome: 10
NCBI: 72273
Homologene: 52813
Genetic Alterations
ENU-induced transitions at the following base pair locations (GRCm38):
  • A to G, chromosome 1 at 37,875,775 bp
  • T to C, chromosome 1 at 53,308,957 bp
  • TTTTTTTTTTTT to TTTTTTTTTTTTTTTTT, chromosome 1 at 85,644,570 bp
  • T to C, chromosome 2 at 12,382,129 bp
  • G to A, chromosome 2 at 157,114,297 bp
  • A to T, chromosome 3 at 99,216,800 bp
  • A to G, chromosome 4 at 42,791,750 bp
  • A to G, chromosome 4 at 43,706,334 bp
  • A to C, chromosome 4 at 88,755,488 bp
  • T to C, chromosome 4 at 103,266,411 bp
  • G to C, chromosome 4 at 141,000,854 bp
  • T to G, chromosome 5 at 13,795,448 bp
  • C to T, chromosome 5 at 124,584,297 bp
  • T to C, chromosome 5 at 124,758,355 bp
  • A to G, chromosome 5 at 143,963,873 bp
  • A to G, chromosome 6 at 41,314,679 bp
  • T to A, chromosome 6 at 70,138,513 bp
  • A to T, chromosome 6 at 101,150,514 bp
  • T to A, chromosome 6 at 124,521,575 bp
  • T to A, chromosome 6 at 134,921,320 bp
  • A to T, chromosome 7 at 4,629,609 bp
  • G to T, chromosome 7 at 85,621,228 bp
  • A to C, chromosome 7 at 109,143,576 bp
  • A to T, chromosome 7 at 119,144,511 bp
  • A to T, chromosome 8 at 22,173,303 bp
  • A to G, chromosome 8 at 86,509,089 bp
  • A to G, chromosome 8 at 116,937,165 bp
  • G to A, chromosome 8 at 124,527,103 bp
  • T to C, chromosome 9 at 20,875,360 bp
  • A to G, chromosome 9 at 62,951,860 bp
  • T to C, chromosome 9 at 106,835,589 bp
  • T to C, chromosome 9 at 108,955,527 bp
  • A to G, chromosome 10 at 81,393,861 bp
  • A to T, chromosome 10 at 130,429,412 bp
  • A to T, chromosome 11 at 22,517,427 bp
  • T to C, chromosome 12 at 50,425,495 bp
  • A to T, chromosome 13 at 70,606,309 bp
  • T to C, chromosome 13 at 112,604,547 bp
  • A to G, chromosome 14 at 32,201,637 bp
  • T to C, chromosome 14 at 52,490,620 bp
  • A to T, chromosome 14 at 104,479,065 bp
  • A to T, chromosome 15 at 8,079,337 bp
  • A to G, chromosome 17 at 21,398,130 bp
  • A to T, chromosome 17 at 34,734,205 bp
  • T to A, chromosome 17 at 34,864,118 bp
  • G to T, chromosome 17 at 37,225,268 bp
  • T to C, chromosome 18 at 20,533,422 bp
  • T to A, chromosome 18 at 22,516,340 bp
  • T to A, chromosome 18 at 44,661,811 bp
  • A to T, chromosome 18 at 63,106,271 bp
  • A to G, chromosome 18 at 67,789,449 bp
Phenotype
G1 male heterozygous mice are viable and fertile. The G1 mice were not characterized prior to archiving.
Strain Development
C57BL/6J mice were injected with ENU, resulting in G0 mice that were bred back to C57BL/6J. Sperm was archived from G1 male mice that produced viable G2 mice.
Suggested Control Mice
Wild-type littermates or C57BL/6J mice
MMRRC Genetic QC Summary
The MMRRC Centers have developed a genetic QC pipeline using MiniMUGA array genotyping to provide additional information on strain backgrounds for MMRRC congenic and inbred strains. For more information on when data may be available, or to request genotyping for a strain of interest, please contact mmrrc@missouri.edu. Older strains may not have this information.
Donor
Bruce Beutler, M.D., University of Texas Southwestern Medical Center.
Primary Reference
MUTAGENETIX record for R6518 (G1). B. Beutler and colleagues, Center for the Genetics of Host Defense, UT Southwestern, Dallas, TX.

Colony and Husbandry Information

Colony Surveillance Program and Current Health Reports

Mice recovered from a cryo-archive will have health surveillance performed on recipient females. Health reports will be provided prior to shipment. If you require additional health status information, please email mmrrc@missouri.edu.
Coat Color
Black
MMRRC Breeding System
Random intra-strain mating
Breeding Scheme(s)
When maintaining a live colony, heterozygous mice may be bred together, bred with wild-type siblings, or bred with C57BL/6J inbred mice.
Overall Breeding Performance
Undetermined; during development, mating was done with heterozygous males; viability, fertility and other breeding statistics for heterozygous females and homozygotes are unverified, but presumably would be similar to the C57BL/6J background strain
Average litter size
Undetermined
Recommended wean age
3 weeks

Order Request Information

Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.

Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.

The donor or their institution limits the distribution to non-profit institutions only.

Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.

Click button to Request this one strain. (Use the MMRRC Catalog Search to request more than one strain.)
MMRRC Item # Description Distribution Fee / Unit (US $)
*Shipping & Handling not included*
Units Notes
044645-MU-RESUS Litter recovered from cryo-archive $5,475.00 / Non-Profit Litter Recovered litter4; additional fees for any special requests.
Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

1 The distribution fee covers the expense of rederiving mice from a live mouse; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

2 An aliquot contains a sufficient number of embryos (in one or more vials or straws and based on the transfer success rate of the MMRRC facility) to transfer into one to three recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.

3 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.

4 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.