Strain Name:
C57BL/6J-MtgxR6521Btlr/Mmmh
Stock Number:
044647-MU
Citation ID:
RRID:MMRRC_044647-MU
Other Names:
R6521 (G1)
Major Collection:

Strain Information

Erbb4
Name: erb-b2 receptor tyrosine kinase 4
Synonyms: Her4, ErbB4
Type: Gene
Species: Mouse
Chromosome: 1
NCBI: 13869
HGNC: HGNC:3432
Homologene: 21084
Sptan1
Name: spectrin alpha, non-erythrocytic 1
Synonyms: alphaII-spectrin, 2610027H02Rik, Spna-2, Spna2, alpha-fodrin
Type: Gene
Species: Mouse
Chromosome: 2
NCBI: 20740
Homologene: 2353
Ptma
Name: prothymosin alpha
Synonyms: Thym
Type: Gene
Species: Mouse
Chromosome: 1
NCBI: 19231
HGNC: HGNC:9623
Homologene: 137217
Swap70
Name: SWA-70 protein
Synonyms: 70kDa
Type: Gene
Species: Mouse
Chromosome: 7
NCBI: 20947
Homologene: 7557
Ankfy1
Name: ankyrin repeat and FYVE domain containing 1
Synonyms: Ankhzn
Type: Gene
Species: Mouse
Chromosome: 11
NCBI: 11736
Homologene: 9491
Zfp407
Name: zinc finger protein 407
Synonyms: LOC240469, 6430585N13Rik, LOC381139
Type: Gene
Species: Mouse
Chromosome: 18
NCBI: 240476
Homologene: 86402
Senp1
Name: SUMO1/sentrin specific peptidase 1
Synonyms: E330036L07Rik, D15Ertd528e, 2310046A20Rik
Type: Gene
Species: Mouse
Chromosome: 15
NCBI: 223870
Homologene: 8731
Ank3
Name: ankyrin 3, epithelial
Synonyms: Ank-3, AnkG, 2900054D09Rik, Ankyrin-3, Ankyrin-G
Type: Gene
Species: Mouse
Chromosome: 10
NCBI: 11735
HGNC: HGNC:494
Homologene: 56908
Slc12a3
Name: solute carrier family 12, member 3
Synonyms: TSC, NCC
Type: Gene
Species: Mouse
Chromosome: 8
NCBI: 20497
Homologene: 287
Cyfip2
Name: cytoplasmic FMR1 interacting protein 2
Synonyms: 1500004I01Rik, 6430511D02Rik, Pir121
Type: Gene
Species: Mouse
Chromosome: 11
NCBI: 76884
Homologene: 7936
Unc5a
Name: unc-5 netrin receptor A
Synonyms: Unc5h1
Type: Gene
Species: Mouse
Chromosome: 13
NCBI: 107448
Homologene: 41474
Aars1
Name: alanyl-tRNA synthetase 1
Synonyms: Aars
Type: Gene
Species: Mouse
Chromosome: 8
NCBI: 234734
HGNC: HGNC:20
Homologene: 1213
Crebbp
Name: CREB binding protein
Synonyms: KAT3A, CBP
Type: Gene
Species: Mouse
Chromosome: 16
NCBI: 12914
HGNC: HGNC:2348
Homologene: 68393
Acsbg2
Name: acyl-CoA synthetase bubblegum family member 2
Synonyms: Bgr
Type: Gene
Species: Mouse
Chromosome: 17
NCBI: 328845
Homologene: 57133
Mkln1
Name: muskelin 1, intracellular mediator containing kelch motifs
Synonyms: A130067F06Rik
Type: Gene
Species: Mouse
Chromosome: 6
NCBI: 27418
HGNC: HGNC:7109
Homologene: 8305
Mtmr4
Name: myotubularin related protein 4
Synonyms: FYVE zinc finger phosphatase, ESTM44, ZFYVE11, FYVE-DSP2
Type: Gene
Species: Mouse
Chromosome: 11
NCBI: 170749
HGNC: HGNC:7452
Homologene: 3440
Myo15a
Name: myosin XVA
Synonyms: Myo15
Type: Gene
Species: Mouse
Chromosome: 11
NCBI: 17910
HGNC: HGNC:7594
Homologene: 56504
Sirpa
Name: signal-regulatory protein alpha
Synonyms: Idd13.2, Bit, Ptpns1, SHPS-1, SIRP, CD172a, P84
Type: Gene
Species: Mouse
Chromosome: 2
NCBI: 19261
HGNC: HGNC:9662
Homologene: 7246
Ano4
Name: anoctamin 4
Synonyms: A330096O15Rik, Tmem16d
Type: Gene
Species: Mouse
Chromosome: 10
NCBI: 320091
Homologene: 66599
Piezo2
Name: piezo-type mechanosensitive ion channel component 2
Synonyms: Piezo2, Fam38b, 9030411M15Rik, Fam38b2, 9430028L06Rik
Type: Gene
Species: Mouse
Chromosome: 18
NCBI: 667742
Homologene: 49695
Fsip2
Name: fibrous sheath-interacting protein 2
Synonyms: OTTMUSG00000013335
Type: Gene
Species: Mouse
Chromosome: 2
NCBI: 241516
Homologene: 110349
Adgrv1
Name: adhesion G protein-coupled receptor V1
Synonyms: Mgr1, Mass1, Gpr98, VLGR1
Type: Gene
Species: Mouse
Chromosome: 13
NCBI: 110789
Homologene: 19815
Nckap5
Name: NCK-associated protein 5
Synonyms: E030049G20Rik, D130011D22Rik, LOC380609
Type: Gene
Species: Mouse
Chromosome: 1
NCBI: 210356
Homologene: 35542
Muc5b
Name: mucin 5, subtype B, tracheobronchial
Synonyms: MUC5, 2300002I04Rik, MUC9
Type: Gene
Species: Mouse
Chromosome: 7
NCBI: 74180
HGNC: HGNC:7516
Homologene: 136756
Or11j4
Name: olfactory receptor family 11 subfamily J member 4
Synonyms: MOR106-5, GA_x6K02T2PMLR-6089963-6090901, Olfr736
Type: Gene
Species: Mouse
Chromosome: 14
NCBI: 258660
Homologene: 64902
Prss1
Name: serine protease 1 (trypsin 1)
Synonyms: PRSS1, Try-1, Try1, Trygn16
Type: Gene
Species: Mouse
Chromosome: 6
NCBI: 114228
Homologene: 88408
Rem2
Name: rad and gem related GTP binding protein 2
Type: Gene
Species: Mouse
Chromosome: 14
NCBI: 140743
Homologene: 11222
Tcaf3
Name: TRPM8 channel-associated factor 3
Synonyms: Eapa2, Fam115e
Type: Gene
Species: Mouse
Chromosome: 6
NCBI: 403088
Homologene: 74374
Cdh20
Name: cadherin 20
Synonyms: Cdh7
Type: Gene
Species: Mouse
Chromosome: 1
NCBI: 23836
HGNC: HGNC:1760
Homologene: 8015
Rab39
Name: RAB39, member RAS oncogene family
Synonyms: Rab39a, C230094F14Rik
Type: Gene
Species: Mouse
Chromosome: 9
NCBI: 270160
Homologene: 56773
Mpl
Name: myeloproliferative leukemia virus oncogene
Synonyms: c-mpl-II, CD110, c-mpl-I, hlb219, TPO-R, thrombopoietin receptor, c-mpl
Type: Gene
Species: Mouse
Chromosome: 4
NCBI: 17480
HGNC: HGNC:7217
Homologene: 7845
Slc22a14
Name: solute carrier family 22 (organic cation transporter), member 14
Synonyms: LOC382113
Type: Gene
Species: Mouse
Chromosome: 9
NCBI: 382113
HGNC: HGNC:8495
Homologene: 3530
Klhl18
Name: kelch-like 18
Type: Gene
Species: Mouse
Chromosome: 9
NCBI: 270201
Homologene: 14805
Ceacam5
Name: CEA cell adhesion molecule 5
Synonyms: 1600029H12Rik, Psg30
Type: Gene
Species: Mouse
Chromosome: 7
NCBI: 73250
HGNC: HGNC:1819
Homologene: 115938
Nfxl1
Name: nuclear transcription factor, X-box binding-like 1
Synonyms: D430033A06Rik, TCF9, 1700012H24Rik, LOC381696
Type: Gene
Species: Mouse
Chromosome: 5
NCBI: 100978
Homologene: 26752
Mdfic
Name: MyoD family inhibitor domain containing
Synonyms: Kdt1, clone 1.5
Type: Gene
Species: Mouse
Chromosome: 6
NCBI: 16543
Homologene: 18404
Slfn5
Name: schlafen 5
Type: Gene
Species: Mouse
Chromosome: 11
NCBI: 327978
Homologene: 18839
Or8d2
Name: olfactory receptor family 8 subfamily D member 2
Synonyms: GA_x6K02T2PVTD-32543982-32544908, Olfr1520-ps1, MOR171-27P, MOR171-47, MOR171-27P, Olfr924
Type: Gene
Species: Mouse
Chromosome: 9
NCBI: 404322
VEGA: 9
HGNC: HGNC:8482
Homologene: 128215
Mmd2
Name: monocyte to macrophage differentiation-associated 2
Type: Gene
Species: Mouse
Chromosome: 5
NCBI: 75104
Homologene: 18425
Klhdc3
Name: kelch domain containing 3
Synonyms: Peas, 1300011D16Rik
Type: Gene
Species: Mouse
Chromosome: 17
NCBI: 71765
VEGA: 17
Homologene: 14290
Or4c11c
Name: olfactory receptor family 4 subfamily C member 11C
Synonyms: MOR230-3, Olfr1205, Olfr1203, MOR230-1, GA_x6K02T2Q125-50304328-50305251, GA_x6K02T2Q125-50336588-50337313
Type: Gene
Species: Mouse
Chromosome: 2
NCBI: 258898
Homologene: 81567
Hoxc8
Name: homeobox C8
Synonyms: Hox-3.1, D130011F21Rik
Type: Gene
Species: Mouse
Chromosome: 15
NCBI: 15426
HGNC: HGNC:5129
Homologene: 130642
Celf4
Name: CUGBP, Elav-like family member 4
Synonyms: Brunol4, C130060B05Rik, Brul4, A230070D14Rik, BRUNOL-4
Type: Gene
Species: Mouse
Chromosome: 18
NCBI: 108013
VEGA: 18
Homologene: 23202
Catsper2
Name: cation channel, sperm associated 2
Type: Gene
Species: Mouse
Chromosome: 2
NCBI: 212670
Homologene: 77423
Serhl
Name: serine hydrolase-like
Synonyms: 1110019M09Rik
Type: Gene
Species: Mouse
Chromosome: 15
NCBI: 68607
Homologene: 65196
Pigx
Name: phosphatidylinositol glycan anchor biosynthesis, class X
Synonyms: 2010319C14Rik
Type: Gene
Species: Mouse
Chromosome: 16
NCBI: 72084
Homologene: 9886
Tas2r119
Name: taste receptor, type 2, member 119
Synonyms: T2R19, Tas2r19, mt2r19, mGR19
Type: Gene
Species: Mouse
Chromosome: 15
NCBI: 57254
Homologene: 10480
Cfap91
Name: cilia and flagella associated protein 91
Synonyms: Spata26, 4932425I24Rik, Maats1
Type: Gene
Species: Mouse
Chromosome: 16
NCBI: 320214
Homologene: 33470
Or2ah1
Name: olfactory receptor family 2 subfamily AH member 1
Synonyms: Olfr1018, GA_x6K02T2Q125-47301584-47302519, MOR260-5
Type: Gene
Species: Mouse
Chromosome: 2
NCBI: 258579
Homologene: 27216
Traj31
Name: T cell receptor alpha joining 31
Synonyms: Gm17008
Type: Gene
Species: Mouse
Chromosome: 14
NCBI: 100124358
Genetic Alterations
ENU-induced transitions at the following base pair locations (GRCm38):
  • T to A, chromosome 1 at 68,042,530 bp
  • A to G, chromosome 1 at 86,527,847 bp
  • A to C, chromosome 1 at 104,942,134 bp
  • A to T, chromosome 1 at 126,382,172 bp
  • T to C, chromosome 2 at 30,020,455 bp
  • A to G, chromosome 2 at 82,990,086 bp
  • A to T, chromosome 2 at 85,823,450 bp
  • A to G, chromosome 2 at 88,831,356 bp
  • A to G, chromosome 2 at 121,406,807 bp
  • T to G, chromosome 2 at 129,630,155 bp
  • C to T, chromosome 4 at 118,455,117 bp
  • A to T, chromosome 5 at 72,540,308 bp
  • A to G, chromosome 5 at 142,574,830 bp
  • T to A, chromosome 6 at 15,729,028 bp
  • T to A, chromosome 6 at 31,490,544 bp
  • C to T, chromosome 6 at 41,463,681 bp
  • T to A, chromosome 6 at 42,593,238 bp
  • T to C, chromosome 7 at 17,750,831 bp
  • T to C, chromosome 7 at 110,255,820 bp
  • C to A, chromosome 7 at 141,859,171 bp
  • T to C, chromosome 8 at 94,343,113 bp
  • T to A, chromosome 8 at 111,043,336 bp
  • C to T, chromosome 9 at 38,848,597 bp
  • T to C, chromosome 9 at 53,706,031 bp
  • A to G, chromosome 9 at 110,428,635 bp
  • T to C, chromosome 9 at 119,220,769 bp
  • A to G, chromosome 10 at 69,992,766 bp
  • A to G, chromosome 10 at 88,983,778 bp
  • A to T, chromosome 11 at 46,254,588 bp
  • C to T, chromosome 11 at 60,502,369 bp
  • G to A, chromosome 11 at 72,730,482 bp
  • A to G, chromosome 11 at 82,960,415 bp
  • A to G, chromosome 11 at 87,613,527 bp
  • T to A, chromosome 13 at 55,004,935 bp
  • A to T, chromosome 13 at 81,433,652 bp
  • T to C, chromosome 14 at 50,393,548 bp
  • A to G, chromosome 14 at 54,187,930 bp
  • C to T, chromosome 14 at 54,477,687 bp
  • G to A, chromosome 15 at 32,178,173 bp
  • A to G, chromosome 15 at 83,101,642 bp
  • A to G, chromosome 15 at 98,048,271 bp
  • G to A, chromosome 15 at 102,992,703 bp
  • A to T, chromosome 16 at 4,119,128 bp
  • A to G, chromosome 16 at 32,087,311 bp
  • A to G, chromosome 16 at 38,306,759 bp
  • A to G, chromosome 17 at 46,677,761 bp
  • T to C, chromosome 17 at 56,861,565 bp
  • T to A, chromosome 18 at 25,479,474 bp
  • T to C, chromosome 18 at 63,021,328 bp
  • T to A, chromosome 18 at 84,432,411 bp
Phenotype
G1 male heterozygous mice are viable and fertile. The G1 mice were not characterized prior to archiving.
Strain Development
C57BL/6J mice were injected with ENU, resulting in G0 mice that were bred back to C57BL/6J. Sperm was archived from G1 male mice that produced viable G2 mice.
Suggested Control Mice
Wild-type littermates or C57BL/6J mice
MMRRC Genetic QC Summary
The MMRRC Centers have developed a genetic QC pipeline using MiniMUGA array genotyping to provide additional information on strain backgrounds for MMRRC congenic and inbred strains. For more information on when data may be available, or to request genotyping for a strain of interest, please contact mmrrc@missouri.edu. Older strains may not have this information.
Donor
Bruce Beutler, M.D., University of Texas Southwestern Medical Center.
Primary Reference
MUTAGENETIX record for R6521 (G1). B. Beutler and colleagues, Center for the Genetics of Host Defense, UT Southwestern, Dallas, TX.

Colony and Husbandry Information

Colony Surveillance Program and Current Health Reports

Mice recovered from a cryo-archive will have health surveillance performed on recipient females. Health reports will be provided prior to shipment. If you require additional health status information, please email mmrrc@missouri.edu.
Coat Color
Black
MMRRC Breeding System
Random intra-strain mating
Breeding Scheme(s)
When maintaining a live colony, heterozygous mice may be bred together, bred with wild-type siblings, or bred with C57BL/6J inbred mice.
Overall Breeding Performance
Undetermined; during development, mating was done with heterozygous males; viability, fertility and other breeding statistics for heterozygous females and homozygotes are unverified, but presumably would be similar to the C57BL/6J background strain
Average litter size
Undetermined
Recommended wean age
3 weeks

Order Request Information

Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.

Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.

The donor or their institution limits the distribution to non-profit institutions only.

Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.

Click button to Request this one strain. (Use the MMRRC Catalog Search to request more than one strain.)
MMRRC Item # Description Distribution Fee / Unit (US $)
*Shipping & Handling not included*
Units Notes
044647-MU-RESUS Litter recovered from cryo-archive $5,475.00 / Non-Profit Litter Recovered litter4; additional fees for any special requests.
Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

1 The distribution fee covers the expense of rederiving mice from a live mouse; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

2 An aliquot contains a sufficient number of embryos (in one or more vials or straws and based on the transfer success rate of the MMRRC facility) to transfer into one to three recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.

3 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.

4 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.