Strain Name:
C57BL/6J-MtgxR6527Btlr/Mmmh
Stock Number:
044653-MU
Citation ID:
RRID:MMRRC_044653-MU
Other Names:
R6527 (G1)
Major Collection:

Strain Information

Tmem59l
Name: transmembrane protein 59-like
Synonyms: 5330410G16Rik
Type: Gene
Species: Mouse
Chromosome: 8
NCBI: 67937
Homologene: 8104
Ppp1r9a
Name: protein phosphatase 1, regulatory subunit 9A
Synonyms: NRB, A230094E16Rik, neurabin-I, 2810430P21Rik, Neurabin I, 4930518N04Rik
Type: Gene
Species: Mouse
Chromosome: 6
NCBI: 243725
Homologene: 14247
Mif4gd
Name: MIF4G domain containing
Synonyms: 2310075G12Rik, 1110014L05Rik
Type: Gene
Species: Mouse
Chromosome: 11
NCBI: 69674
Homologene: 41389
Adgrl3
Name: adhesion G protein-coupled receptor L3
Synonyms: 5430402I23Rik, Lphn3, D130075K09Rik, LEC3, lectomedin 3
Type: Gene
Species: Mouse
Chromosome: 5
NCBI: 319387
Homologene: 22878
Mast2
Name: microtubule associated serine/threonine kinase 2
Synonyms: Mtssk, MAST205
Type: Gene
Species: Mouse
Chromosome: 4
NCBI: 17776
Homologene: 7428
Dscaml1
Name: DS cell adhesion molecule like 1
Synonyms: 4930435C18Rik, 4921507G06Rik
Type: Gene
Species: Mouse
Chromosome: 9
NCBI: 114873
VEGA: 9
Homologene: 79549
Psme4
Name: proteasome (prosome, macropain) activator subunit 4
Type: Gene
Species: Mouse
Chromosome: 11
NCBI: 103554
Homologene: 113742
Tnks
Name: tankyrase, TRF1-interacting ankyrin-related ADP-ribose polymerase
Synonyms: 4930554K12Rik, Parp5a, D130072O21Rik, tankyrase 1, TANK1, mTNKS1
Type: Gene
Species: Mouse
Chromosome: 8
NCBI: 21951
Homologene: 18405
Osbpl8
Name: oxysterol binding protein-like 8
Synonyms: D330025H14Rik, ORP-8
Type: Gene
Species: Mouse
Chromosome: 10
NCBI: 237542
VEGA: 10
Homologene: 68813
Psmd12
Name: proteasome (prosome, macropain) 26S subunit, non-ATPase, 12
Synonyms: 1500002F15Rik, P55
Type: Gene
Species: Mouse
Chromosome: 11
NCBI: 66997
HGNC: HGNC:9557
Homologene: 2109
Jam3
Name: junction adhesion molecule 3
Synonyms: JAM-3, Jcam3, 1110002N23Rik, JAM-C
Type: Gene
Species: Mouse
Chromosome: 9
NCBI: 83964
Homologene: 11338
Msr1
Name: macrophage scavenger receptor 1
Synonyms: SR-AI, MRS-A, Scvr, Scara1, MSR-A, SR-AII
Type: Gene
Species: Mouse
Chromosome: 8
NCBI: 20288
HGNC: HGNC:7376
Homologene: 12822
Tmem161b
Name: transmembrane protein 161B
Synonyms: 2810446P07Rik
Type: Gene
Species: Mouse
Chromosome: 13
NCBI: 72745
Homologene: 14519
Wwc1
Name: WW, C2 and coiled-coil domain containing 1
Synonyms: Kibra
Type: Gene
Species: Mouse
Chromosome: 11
NCBI: 211652
Homologene: 69180
Zzef1
Name: zinc finger, ZZ-type with EF hand domain 1
Synonyms: 8430405D05Rik, C130099L13Rik
Type: Gene
Species: Mouse
Chromosome: 11
NCBI: 195018
Homologene: 9027
Smc5
Name: structural maintenance of chromosomes 5
Synonyms: Smc5l1
Type: Gene
Species: Mouse
Chromosome: 19
NCBI: 226026
VEGA: 19
Homologene: 41009
Dsp
Name: desmoplakin
Synonyms: 2300002E22Rik, 5730453H04Rik, rul, DP
Type: Gene
Species: Mouse
Chromosome: 13
NCBI: 109620
HGNC: HGNC:3052
Homologene: 37922
Tomt
Name: transmembrane O-methyltransferase
Synonyms: Comt2, F930017I19Rik
Type: Gene
Species: Mouse
Chromosome: 7
NCBI: 791260
Homologene: 19664
Podxl2
Name: podocalyxin-like 2
Synonyms: Endoglycan, D130074J02Rik, PODLX2
Type: Gene
Species: Mouse
Chromosome: 6
NCBI: 319655
Homologene: 9254
Slc15a4
Name: solute carrier family 15, member 4
Synonyms: PTR4, C130069N12Rik, PHT1
Type: Gene
Species: Mouse
Chromosome: 5
NCBI: 100561
Homologene: 26432
Dhx29
Name: DExH-box helicase 29
Synonyms: DEAH (Asp-Glu-Ala-His) box polypeptide 29, E130202M19Rik
Type: Gene
Species: Mouse
Chromosome: 13
NCBI: 218629
VEGA: 13
Homologene: 10387
Trim37
Name: tripartite motif-containing 37
Synonyms: TEF3, 2810004E07Rik, MUL, 1110032A10Rik
Type: Gene
Species: Mouse
Chromosome: 11
NCBI: 68729
HGNC: HGNC:7523
Homologene: 9084
Zfp984
Name: zinc finger protein 984
Synonyms: Gm13157
Type: Gene
Species: Mouse
Chromosome: 4
NCBI: 100041677
Homologene: 133076
Abca16
Name: ATP-binding cassette, sub-family A member 16
Type: Gene
Species: Mouse
Chromosome: 7
NCBI: 233810
Homologene: 132942
Abcb6
Name: ATP-binding cassette, sub-family B member 6
Synonyms: 1200005B17Rik
Type: Gene
Species: Mouse
Chromosome: 1
NCBI: 74104
HGNC: HGNC:47
Homologene: 11375
Cfap74
Name: cilia and flagella associated protein 74
Synonyms: 2010015L04Rik
Type: Gene
Species: Mouse
Chromosome: 4
NCBI: 544678
Homologene: 129584
Muc4
Name: mucin 4
Synonyms: 4933405I11Rik
Type: Gene
Species: Mouse
Chromosome: 16
NCBI: 140474
HGNC: HGNC:7514
Homologene: 124469
Sycp1
Name: synaptonemal complex protein 1
Synonyms: SCP1
Type: Gene
Species: Mouse
Chromosome: 3
NCBI: 20957
Homologene: 2389
Vsig8
Name: V-set and immunoglobulin domain containing 8
Synonyms: EG240916
Type: Gene
Species: Mouse
Chromosome: 1
NCBI: 240916
Homologene: 18616
Jakmip2
Name: janus kinase and microtubule interacting protein 2
Synonyms: 6430702L21Rik, D930046L20Rik
Type: Gene
Species: Mouse
Chromosome: 18
NCBI: 76217
VEGA: 18
Homologene: 8866
Gbe1
Name: 1,4-alpha-glucan branching enzyme 1
Synonyms: 2810426P10Rik, 2310045H19Rik, D16Ertd536e
Type: Gene
Species: Mouse
Chromosome: 16
NCBI: 74185
HGNC: HGNC:4180
Homologene: 129
Rab11fip1
Name: RAB11 family interacting protein 1 (class I)
Synonyms: 4833414G05Rik, 2010200K21Rik
Type: Gene
Species: Mouse
Chromosome: 8
NCBI: 75767
Homologene: 11853
Duox2
Name: dual oxidase 2
Synonyms: A430065P05Rik
Type: Gene
Species: Mouse
Chromosome: 2
NCBI: 214593
Homologene: 9689
Ros1
Name: Ros1 proto-oncogene
Synonyms: c-ros, Ros-1
Type: Gene
Species: Mouse
Chromosome: 10
NCBI: 19886
Homologene: 2207
Vwa8
Name: von Willebrand factor A domain containing 8
Synonyms: 1300010F03Rik, 4932416F07Rik
Type: Gene
Species: Mouse
Chromosome: 14
NCBI: 219189
VEGA: 14
Homologene: 44881
Letm2
Name: leucine zipper-EF-hand containing transmembrane protein 2
Synonyms: D030041N04Rik
Type: Gene
Species: Mouse
Chromosome: 8
NCBI: 270035
Homologene: 56358
Or11j4
Name: olfactory receptor family 11 subfamily J member 4
Synonyms: Olfr736, MOR106-5, GA_x6K02T2PMLR-6089963-6090901
Type: Gene
Species: Mouse
Chromosome: 14
NCBI: 258660
Homologene: 64902
Or13p3
Name: olfactory receptor family 13 subfamily P member 3
Synonyms: MOR258-1, GA_x6K02T2QD9B-18838170-18837232, Olfr1341
Type: Gene
Species: Mouse
Chromosome: 4
NCBI: 258852
Homologene: 27281
Lmod3
Name: leiomodin 3 (fetal)
Synonyms: 5430424A14Rik
Type: Gene
Species: Mouse
Chromosome: 6
NCBI: 320502
HGNC: HGNC:6649
Homologene: 28097
Or1n1b
Name: olfactory receptor family 1 subfamily N member 1B
Synonyms: GA_x6K02T2NLDC-33585366-33584431, MOR127-3, Olfr353
Type: Gene
Species: Mouse
Chromosome: 2
NCBI: 258943
HGNC: HGNC:8221
Homologene: 115503
Mtus2
Name: microtubule associated tumor suppressor candidate 2
Synonyms: A730013O20Rik, C130038G02Rik, 5730592G18Rik
Type: Gene
Species: Mouse
Chromosome: 5
NCBI: 77521
Homologene: 78141
Dlg5
Name: discs large MAGUK scaffold protein 5
Synonyms: 4933429D20Rik
Type: Gene
Species: Mouse
Chromosome: 14
NCBI: 71228
HGNC: HGNC:2904
Homologene: 3486
Sqor
Name: sulfide quinone oxidoreductase
Synonyms: 0610039J17Rik, flavo-binding protein, 4930557M22Rik, Sqrdl
Type: Gene
Species: Mouse
Chromosome: 2
NCBI: 59010
Homologene: 10921
Vmn1r56
Name: vomeronasal 1 receptor 56
Synonyms: V1rd3, V3R3
Type: Gene
Species: Mouse
Chromosome: 7
NCBI: 81015
Homologene: 41799
Nudt14
Name: nudix hydrolase 14
Synonyms: 1110030M18Rik, nudix (nucleoside diphosphate linked moiety X)-type motif 14
Type: Gene
Species: Mouse
Chromosome: 12
NCBI: 66174
VEGA: 12
Homologene: 11930
Steap4
Name: STEAP family member 4
Synonyms: Tiarp, Tnfaip9
Type: Gene
Species: Mouse
Chromosome: 5
NCBI: 117167
Homologene: 36422
V1ra8
Name: vomeronasal 1 receptor, A8
Synonyms: Vmn1r-ps33
Type: Gene
Species: Mouse
Chromosome: 6
NCBI: 113850
Prss42
Name: serine protease 42
Synonyms: Tessp2
Type: Gene
Species: Mouse
Chromosome: 9
NCBI: 235628
Homologene: 27410
Flacc1
Name: flagellum associated containing coiled-coil domains 1
Synonyms: Als2cr12, 4933425F06Rik, 4933405P16Rik
Type: Gene
Species: Mouse
Chromosome: 1
NCBI: 108812
Homologene: 51399
Heatr4
Name: HEAT repeat containing 4
Synonyms: Gm17673
Type: Gene
Species: Mouse
Chromosome: 12
NCBI: 101055670
Homologene: 45728
Amd2
Name: S-adenosylmethionine decarboxylase 2
Synonyms: Amd3, ENSMUSG00000063953
Type: Gene
Species: Mouse
Chromosome: 10
NCBI: 100041585
VEGA: 10
HGNC: HGNC:457
Homologene: 1235
Genetic Alterations
ENU-induced transitions at the following base pair locations (GRCm38):
  • T to C, chromosome 1 at 58,692,413 bp
  • T to C, chromosome 1 at 75,177,488 bp
  • T to C, chromosome 1 at 172,560,358 bp
  • T to C, chromosome 2 at 36,890,582 bp
  • A to G, chromosome 2 at 122,294,614 bp
  • A to G, chromosome 2 at 122,809,286 bp
  • A to G, chromosome 3 at 102,898,887 bp
  • A to G, chromosome 3 at 138,067,527 bp
  • T to C, chromosome 4 at 116,314,939 bp
  • T to A, chromosome 4 at 118,709,848 bp
  • T to C, chromosome 4 at 147,755,924 bp
  • A to G, chromosome 4 at 155,422,265 bp
  • T to A, chromosome 5 at 7,978,502 bp
  • A to C, chromosome 5 at 81,787,517 bp
  • G to A, chromosome 5 at 127,596,709 bp
  • A to G, chromosome 5 at 148,277,598 bp
  • A to G, chromosome 6 at 5,045,949 bp
  • T to C, chromosome 6 at 88,842,930 bp
  • T to A, chromosome 6 at 90,203,313 bp
  • T to C, chromosome 6 at 97,247,378 bp
  • A to G, chromosome 6 at 121,769,710 bp
  • A to G, chromosome 7 at 5,196,576 bp
  • A to G, chromosome 7 at 101,900,392 bp
  • T to A, chromosome 7 at 120,477,772 bp
  • A to G, chromosome 8 at 25,592,506 bp
  • A to T, chromosome 8 at 27,174,392 bp
  • A to T, chromosome 8 at 34,873,093 bp
  • T to C, chromosome 8 at 39,624,233 bp
  • T to C, chromosome 8 at 70,486,125 bp
  • C to T, chromosome 9 at 27,155,344 bp
  • C to T, chromosome 9 at 45,712,184 bp
  • T to C, chromosome 9 at 110,800,856 bp
  • A to C, chromosome 10 at 35,710,806 bp
  • T to C, chromosome 10 at 52,143,377 bp
  • T to C, chromosome 10 at 111,293,205 bp
  • A to C, chromosome 11 at 30,832,175 bp
  • C to T, chromosome 11 at 35,853,437 bp
  • G to A, chromosome 11 at 72,874,990 bp
  • T to A, chromosome 11 at 83,213,106 bp
  • A to G, chromosome 11 at 87,190,084 bp
  • A to G, chromosome 11 at 107,488,968 bp
  • C to T, chromosome 11 at 115,609,275 bp
  • C to T, chromosome 12 at 83,979,763 bp
  • T to A, chromosome 12 at 112,934,887 bp
  • T to A, chromosome 13 at 38,195,873 bp
  • T to G, chromosome 13 at 84,272,264 bp
  • G to T, chromosome 13 at 112,932,542 bp
  • T to A, chromosome 14 at 24,190,448 bp
  • T to A, chromosome 14 at 50,393,428 bp
  • A to T, chromosome 14 at 78,947,213 bp
  • A to G, chromosome 16 at 32,753,433 bp
  • T to A, chromosome 16 at 70,433,672 bp
  • A to G, chromosome 18 at 43,556,524 bp
  • T to C, chromosome 19 at 23,228,190 bp
Phenotype
G1 male heterozygous mice are viable and fertile. The G1 mice were not characterized prior to archiving.
Strain Development
C57BL/6J mice were injected with ENU, resulting in G0 mice that were bred back to C57BL/6J. Sperm was archived from G1 male mice that produced viable G2 mice.
Suggested Control Mice
Wild-type littermates or C57BL/6J mice
MMRRC Genetic QC Summary
The MMRRC Centers have developed a genetic QC pipeline using MiniMUGA array genotyping to provide additional information on strain backgrounds for MMRRC congenic and inbred strains. For more information on when data may be available, or to request genotyping for a strain of interest, please contact mmrrc@missouri.edu. Older strains may not have this information.
Donor
Bruce Beutler, M.D., University of Texas Southwestern Medical Center.
Primary Reference
MUTAGENETIX record for R6527 (G1). B. Beutler and colleagues, Center for the Genetics of Host Defense, UT Southwestern, Dallas, TX.

Colony and Husbandry Information

Colony Surveillance Program and Current Health Reports

Mice recovered from a cryo-archive will have health surveillance performed on recipient females. Health reports will be provided prior to shipment. If you require additional health status information, please email mmrrc@missouri.edu.
Coat Color
Black
MMRRC Breeding System
Random intra-strain mating
Breeding Scheme(s)
When maintaining a live colony, heterozygous mice may be bred together, bred with wild-type siblings, or bred with C57BL/6J inbred mice.
Overall Breeding Performance
Undetermined; during development, mating was done with heterozygous males; viability, fertility and other breeding statistics for heterozygous females and homozygotes are unverified, but presumably would be similar to the C57BL/6J background strain
Average litter size
Undetermined
Recommended wean age
3 weeks

Order Request Information

Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.

Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.

The donor or their institution limits the distribution to non-profit institutions only.

Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.

Click button to Request this one strain. (Use the MMRRC Catalog Search to request more than one strain.)
MMRRC Item # Description Distribution Fee / Unit (US $)
*Shipping & Handling not included*
Units Notes
044653-MU-RESUS Litter recovered from cryo-archive $5,475.00 / Non-Profit Litter Recovered litter4; additional fees for any special requests.
Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

1 The distribution fee covers the expense of rederiving mice from a live mouse; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

2 An aliquot contains a sufficient number of embryos (in one or more vials or straws and based on the transfer success rate of the MMRRC facility) to transfer into one to three recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.

3 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.

4 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.