Strain Name:
C57BL/6J-MtgxR6528Btlr/Mmmh
Stock Number:
044654-MU
Citation ID:
RRID:MMRRC_044654-MU
Other Names:
R6528 (G1)
Major Collection:

Strain Information

Gtf2h3
Name: general transcription factor IIH, polypeptide 3
Synonyms: 5033417D07Rik, BTF2, D5Ertd679e, 34kDa
Type: Gene
Species: Mouse
Chromosome: 5
NCBI: 209357
HGNC: HGNC:4657
Homologene: 1160
Mycbp2
Name: MYC binding protein 2, E3 ubiquitin protein ligase
Synonyms: Pam, C130061D10Rik, Phr1
Type: Gene
Species: Mouse
Chromosome: 14
NCBI: 105689
Homologene: 9005
Fbxo21
Name: F-box protein 21
Synonyms: 2810425J22Rik
Type: Gene
Species: Mouse
Chromosome: 5
NCBI: 231670
Homologene: 14145
Fkbp15
Name: FK506 binding protein 15
Synonyms: FKBP133, C430014M02Rik
Type: Gene
Species: Mouse
Chromosome: 4
NCBI: 338355
Homologene: 28743
Trps1
Name: transcriptional repressor GATA binding 1
Synonyms: D15Ertd586e, trichorhinophalangeal syndrome I (human)
Type: Gene
Species: Mouse
Chromosome: 15
NCBI: 83925
Homologene: 8556
Mdn1
Name: midasin AAA ATPase 1
Synonyms: LOC213784, 4833432B22Rik, D4Abb1e
Type: Gene
Species: Mouse
Chromosome: 4
NCBI: 100019
Homologene: 39689
Lpin2
Name: lipin 2
Synonyms: 2610511G02Rik
Type: Gene
Species: Mouse
Chromosome: 17
NCBI: 64898
Homologene: 8769
Rad50
Name: RAD50 double strand break repair protein
Synonyms: Rad50l, Mrell
Type: Gene
Species: Mouse
Chromosome: 11
NCBI: 19360
HGNC: HGNC:9816
Homologene: 38092
Wwc1
Name: WW, C2 and coiled-coil domain containing 1
Synonyms: Kibra
Type: Gene
Species: Mouse
Chromosome: 11
NCBI: 211652
Homologene: 69180
Trio
Name: triple functional domain (PTPRF interacting)
Synonyms: 6720464I07Rik, Solo
Type: Gene
Species: Mouse
Chromosome: 15
NCBI: 223435
VEGA: 15
Homologene: 20847
Vps8
Name: VPS8 CORVET complex subunit
Type: Gene
Species: Mouse
Chromosome: 16
NCBI: 209018
Homologene: 44592
Tgif1
Name: TGFB-induced factor homeobox 1
Synonyms: Tgif
Type: Gene
Species: Mouse
Chromosome: 17
NCBI: 21815
Homologene: 7574
Med13
Name: mediator complex subunit 13
Synonyms: Trap240, 1110067M05Rik, Thrap1
Type: Gene
Species: Mouse
Chromosome: 11
NCBI: 327987
Homologene: 21067
Zfp451
Name: zinc finger protein 451
Synonyms: Kiaa0576-hp, 4933435G09Rik, 4930515K21Rik
Type: Gene
Species: Mouse
Chromosome: 1
NCBI: 98403
Homologene: 9188
Lypd8
Name: LY6/PLAUR domain containing 8
Synonyms: 2210415F13Rik
Type: Gene
Species: Mouse
Chromosome: 11
NCBI: 70163
Homologene: 52815
Cacfd1
Name: calcium channel flower domain containing 1
Synonyms: mFwe, 5930434B04Rik
Type: Gene
Species: Mouse
Chromosome: 2
NCBI: 381356
HGNC: HGNC:1365
Homologene: 41182
Zfp54
Name: zinc finger protein 54
Synonyms: clone 18, KRAB10, Zfp-54, Zfp76
Type: Gene
Species: Mouse
Chromosome: 17
NCBI: 22712
Homologene: 129791
Khsrp
Name: KH-type splicing regulatory protein
Synonyms: 6330409F21Rik, KSRP
Type: Gene
Species: Mouse
Chromosome: 17
NCBI: 16549
VEGA: 17
HGNC: HGNC:6316
Homologene: 2734
Vps26b
Name: VPS26 retromer complex component B
Synonyms: 1810012I05Rik, 2310075A12Rik
Type: Gene
Species: Mouse
Chromosome: 9
NCBI: 69091
VEGA: 9
Homologene: 3601
Ranbp10
Name: RAN binding protein 10
Synonyms: 4432417N03Rik
Type: Gene
Species: Mouse
Chromosome: 8
NCBI: 74334
Homologene: 49639
Eml5
Name: echinoderm microtubule associated protein like 5
Synonyms: C130068M19Rik
Type: Gene
Species: Mouse
Chromosome: 12
NCBI: 319670
VEGA: 12
Homologene: 26807
Robo4
Name: roundabout guidance receptor 4
Synonyms: 1200012D01Rik, Magic roundabout
Type: Gene
Species: Mouse
Chromosome: 9
NCBI: 74144
Homologene: 10397
Arhgap29
Name: Rho GTPase activating protein 29
Synonyms: 6720461J18Rik, Parg1, C76601, B130017I01Rik
Type: Gene
Species: Mouse
Chromosome: 3
NCBI: 214137
Homologene: 3539
Xcr1
Name: chemokine (C motif) receptor 1
Synonyms: XCR1, GPR5, C motif-1/lymphotactin receptor, Ccxcr1
Type: Gene
Species: Mouse
Chromosome: 9
NCBI: 23832
HGNC: HGNC:1625
Homologene: 21095
Gm12887
Name: predicted gene 12887
Type: Gene
Species: Mouse
Chromosome: 4
NCBI: 666927
Homologene: 111034
Chd5
Name: chromodomain helicase DNA binding protein 5
Synonyms: B230399N07Rik, 4930532L22Rik
Type: Gene
Species: Mouse
Chromosome: 4
NCBI: 269610
Homologene: 56712
Or10a3m
Name: olfactory receptor family 10 subfamily A member 3M
Synonyms: GA_x6K02T2PBJ9-11043421-11044365, MOR268-3, Olfr512
Type: Gene
Species: Mouse
Chromosome: 7
NCBI: 258719
HGNC: HGNC:8162
Homologene: 138316
Nrxn3
Name: neurexin III
Synonyms: neurexin III beta, neurexin III alpha, neurexin III beta, neurexin III alpha, 9330112C09Rik, D12Bwg0831e, 4933401A11Rik
Type: Gene
Species: Mouse
Chromosome: 12
NCBI: 18191
HGNC: HGNC:8010
Homologene: 83225
Ccnj
Name: cyclin J
Synonyms: D430039C20Rik
Type: Gene
Species: Mouse
Chromosome: 19
NCBI: 240665
Homologene: 10413
Irgm2
Name: immunity-related GTPase family M member 2
Synonyms: Gtpi, Iigp2
Type: Gene
Species: Mouse
Chromosome: 11
NCBI: 54396
Homologene: 78363
Vmn2r28
Name: vomeronasal 2, receptor 28
Synonyms: EG665255
Type: Gene
Species: Mouse
Chromosome: 7
NCBI: 665255
Homologene: 129605
Adam29
Name: a disintegrin and metallopeptidase domain 29
Type: Gene
Species: Mouse
Chromosome: 8
NCBI: 244486
HGNC: HGNC:207
Homologene: 8607
Shox2
Name: SHOX homeobox 2
Synonyms: Prx3, Og12x, 6330543G17Rik
Type: Gene
Species: Mouse
Chromosome: 3
NCBI: 20429
Homologene: 68535
Cyp3a16
Name: cytochrome P450, family 3, subfamily a, polypeptide 16
Type: Gene
Species: Mouse
Chromosome: 5
NCBI: 13114
HGNC: HGNC:2638
Homologene: 133568
Endou
Name: endonuclease, polyU-specific
Synonyms: Tcl-30, Pp11r
Type: Gene
Species: Mouse
Chromosome: 15
NCBI: 19011
VEGA: 15
Homologene: 48394
Tcl1b5
Name: T cell leukemia/lymphoma 1B, 5
Synonyms: D12Ertd644e
Type: Gene
Species: Mouse
Chromosome: 12
NCBI: 27382
VEGA: 12
Homologene: 83274
Or8k35
Name: olfactory receptor family 8 subfamily K member 35
Synonyms: GA_x6K02T2Q125-48079993-48079157, MOR192-4_p, Olfr1082
Type: Gene
Species: Mouse
Chromosome: 2
NCBI: 404473
Homologene: 104285
Or12j3
Name: olfactory receptor family 12 subfamily J member 3
Synonyms: GA_x6K02T2PBJ9-42523824-42522901, MOR252-2, Olfr530
Type: Gene
Species: Mouse
Chromosome: 7
NCBI: 258512
Homologene: 128384
Pcdhb6
Name: protocadherin beta 6
Synonyms: Pcdhb5B, PcdhbF
Type: Gene
Species: Mouse
Chromosome: 18
NCBI: 93877
HGNC: HGNC:8690
Homologene: 62177
Pramel29
Name: PRAME like 29
Synonyms: C87977
Type: Gene
Species: Mouse
Chromosome: 4
NCBI: 97187
Homologene: 103830
Zar1l
Name: zygote arrest 1-like
Synonyms: LOC545824
Type: Gene
Species: Mouse
Chromosome: 5
NCBI: 545824
Homologene: 85370
Ppp2r2b
Name: protein phosphatase 2, regulatory subunit B, beta
Synonyms: 6330404L05Rik, PP2A-PR55B, PR55-BETA, SCA12, 2900026H06Rik, E130009M08Rik
Type: Gene
Species: Mouse
Chromosome: 18
NCBI: 72930
HGNC: HGNC:9305
Homologene: 55833
Ppl
Name: periplakin
Type: Gene
Species: Mouse
Chromosome: 16
NCBI: 19041
VEGA: 16
HGNC: HGNC:9273
Homologene: 2026
Ttll8
Name: tubulin tyrosine ligase-like family, member 8
Synonyms: 1700019P01Rik
Type: Gene
Species: Mouse
Chromosome: 15
NCBI: 239591
Homologene: 52766
Vmn1r232
Name: vomeronasal 1 receptor 232
Synonyms: V1re4
Type: Gene
Species: Mouse
Chromosome: 17
NCBI: 171227
Homologene: 121611
Plekho1
Name: pleckstrin homology domain containing, family O member 1
Synonyms: JZA-20, Jza2, 2810052M02Rik, CKIP-1
Type: Gene
Species: Mouse
Chromosome: 3
NCBI: 67220
Homologene: 9448
Prickle4
Name: prickle planar cell polarity protein 4
Synonyms: LOC381104
Type: Gene
Species: Mouse
Chromosome: 17
NCBI: 381104
Homologene: 22752
Chad
Name: chondroadherin
Synonyms: SLRR4A
Type: Gene
Species: Mouse
Chromosome: 11
NCBI: 12643
HGNC: HGNC:1909
Homologene: 36063
Or13a22
Name: olfactory receptor family 13 subfamily A member 22
Synonyms: GA_x6K02T2PBJ9-42641642-42642574, MOR253-7, Olfr535
Type: Gene
Species: Mouse
Chromosome: 7
NCBI: 258956
Homologene: 138320
Tmem128
Name: transmembrane protein 128
Synonyms: 2810021O14Rik
Type: Gene
Species: Mouse
Chromosome: 5
NCBI: 66309
Homologene: 11944
Cmtm1
Name: CKLF-like MARVEL transmembrane domain containing 1
Synonyms: CHLFH1a, CKLFH1, Cklfsf1
Type: Gene
Species: Mouse
Chromosome: 8
NCBI: 100504164
Homologene: 134399
Pnma1
Name: paraneoplastic antigen MA1
Synonyms: 5730402C15Rik
Type: Gene
Species: Mouse
Chromosome: 12
NCBI: 70481
HGNC: HGNC:9158
Homologene: 4399
Genetic Alterations
ENU-induced transitions at the following base pair locations (GRCm38):
  • A to G, chromosome 1 at 33,777,781 bp
  • T to G, chromosome 2 at 27,018,939 bp
  • T to C, chromosome 2 at 86,594,465 bp
  • T to A, chromosome 2 at 177,193,508 bp
  • C to A, chromosome 3 at 66,981,285 bp
  • C to T, chromosome 3 at 95,989,321 bp
  • T to A, chromosome 3 at 122,014,702 bp
  • G to T, chromosome 4 at 32,713,780 bp
  • A to G, chromosome 4 at 62,332,270 bp
  • C to A, chromosome 4 at 121,615,637 bp
  • A to G, chromosome 4 at 144,208,811 bp
  • T to C, chromosome 4 at 152,356,676 bp
  • T to A, chromosome 5 at 38,266,499 bp
  • C to A, chromosome 5 at 118,000,356 bp
  • A to G, chromosome 5 at 119,883,111 bp
  • C to T, chromosome 5 at 124,584,297 bp
  • C to T, chromosome 5 at 145,440,431 bp
  • T to A, chromosome 5 at 150,507,130 bp
  • T to A, chromosome 7 at 5,490,685 bp
  • A to T, chromosome 7 at 103,250,755 bp
  • T to C, chromosome 7 at 108,713,431 bp
  • G to T, chromosome 7 at 140,373,441 bp
  • A to G, chromosome 7 at 140,493,051 bp
  • C to T, chromosome 8 at 55,872,561 bp
  • T to C, chromosome 8 at 104,309,295 bp
  • T to C, chromosome 8 at 105,779,956 bp
  • C to G, chromosome 9 at 27,010,466 bp
  • T to C, chromosome 9 at 37,404,368 bp
  • A to T, chromosome 9 at 123,855,983 bp
  • C to T, chromosome 11 at 35,853,437 bp
  • G to A, chromosome 11 at 53,652,282 bp
  • C to T, chromosome 11 at 58,220,052 bp
  • G to A, chromosome 11 at 58,384,613 bp
  • G to A, chromosome 11 at 86,298,954 bp
  • A to G, chromosome 11 at 94,565,624 bp
  • T to A, chromosome 12 at 84,147,423 bp
  • C to T, chromosome 12 at 89,513,049 bp
  • T to C, chromosome 12 at 98,824,637 bp
  • A to G, chromosome 12 at 105,178,999 bp
  • T to C, chromosome 14 at 103,142,881 bp
  • A to G, chromosome 15 at 27,805,870 bp
  • A to G, chromosome 15 at 50,822,427 bp
  • T to C, chromosome 15 at 76,174,430 bp
  • G to T, chromosome 15 at 88,914,238 bp
  • C to T, chromosome 15 at 97,719,629 bp
  • T to A, chromosome 16 at 5,087,616 bp
  • C to A, chromosome 16 at 21,554,125 bp
  • G to A, chromosome 17 at 20,914,047 bp
  • G to T, chromosome 17 at 21,433,474 bp
  • T to A, chromosome 17 at 47,689,333 bp
  • G to A, chromosome 17 at 57,023,543 bp
  • G to A, chromosome 17 at 70,846,560 bp
  • T to A, chromosome 17 at 71,244,005 bp
  • A to G, chromosome 18 at 37,334,503 bp
  • T to A, chromosome 18 at 42,688,338 bp
  • C to A, chromosome 19 at 40,832,085 bp
Phenotype
G1 male heterozygous mice are viable and fertile. The G1 mice were not characterized prior to archiving.
Strain Development
C57BL/6J mice were injected with ENU, resulting in G0 mice that were bred back to C57BL/6J. Sperm was archived from G1 male mice that produced viable G2 mice.
Suggested Control Mice
Wild-type littermates or C57BL/6J mice
MMRRC Genetic QC Summary
The MMRRC Centers have developed a genetic QC pipeline using MiniMUGA array genotyping to provide additional information on strain backgrounds for MMRRC congenic and inbred strains. For more information on when data may be available, or to request genotyping for a strain of interest, please contact mmrrc@missouri.edu. Older strains may not have this information.
Donor
Bruce Beutler, M.D., University of Texas Southwestern Medical Center.
Primary Reference
MUTAGENETIX record for R6528 (G1). B. Beutler and colleagues, Center for the Genetics of Host Defense, UT Southwestern, Dallas, TX.

Colony and Husbandry Information

Colony Surveillance Program and Current Health Reports

Mice recovered from a cryo-archive will have health surveillance performed on recipient females. Health reports will be provided prior to shipment. If you require additional health status information, please email mmrrc@missouri.edu.
Coat Color
Black
MMRRC Breeding System
Random intra-strain mating
Breeding Scheme(s)
When maintaining a live colony, heterozygous mice may be bred together, bred with wild-type siblings, or bred with C57BL/6J inbred mice.
Overall Breeding Performance
Undetermined; during development, mating was done with heterozygous males; viability, fertility and other breeding statistics for heterozygous females and homozygotes are unverified, but presumably would be similar to the C57BL/6J background strain
Average litter size
Undetermined
Recommended wean age
3 weeks

Order Request Information

Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.

Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.

The donor or their institution limits the distribution to non-profit institutions only.

Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.

Click button to Request this one strain. (Use the MMRRC Catalog Search to request more than one strain.)
MMRRC Item # Description Distribution Fee / Unit (US $)
*Shipping & Handling not included*
Units Notes
044654-MU-RESUS Litter recovered from cryo-archive $5,475.00 / Non-Profit Litter Recovered litter4; additional fees for any special requests.
Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

1 The distribution fee covers the expense of rederiving mice from a live mouse; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

2 An aliquot contains a sufficient number of embryos (in one or more vials or straws and based on the transfer success rate of the MMRRC facility) to transfer into one to three recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.

3 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.

4 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.