Strain Name:
C57BL/6J-MtgxR6534Btlr/Mmmh
Stock Number:
044660-MU
Citation ID:
RRID:MMRRC_044660-MU
Other Names:
R6534 (G1)
Major Collection:

Strain Information

Prrc1
Name: proline-rich coiled-coil 1
Synonyms: 9430085A19Rik, 3110038B19Rik, 1190002C06Rik, 2310058D16Rik
Type: Gene
Species: Mouse
Chromosome: 18
NCBI: 73137
VEGA: 18
Homologene: 12491
Tnpo3
Name: transportin 3
Synonyms: D6Ertd313e, 5730544L10Rik, C430013M08Rik
Type: Gene
Species: Mouse
Chromosome: 6
NCBI: 320938
Homologene: 40848
Ddx10
Name: DEAD box helicase 10
Synonyms: DEAD (Asp-Glu-Ala-Asp) box polypeptide 10, 4632415A01Rik
Type: Gene
Species: Mouse
Chromosome: 9
NCBI: 77591
VEGA: 9
HGNC: HGNC:2735
Homologene: 20922
Atp2a2
Name: ATPase, Ca++ transporting, cardiac muscle, slow twitch 2
Synonyms: D5Wsu150e, Serca2a, SERCA2, 9530097L16Rik, SERCA2B, sarco/endoplasmic reticulum Ca2+-ATPase 2
Type: Gene
Species: Mouse
Chromosome: 5
NCBI: 11938
HGNC: HGNC:812
Homologene: 80167
Cdk5rap2
Name: CDK5 regulatory subunit associated protein 2
Synonyms: an, 2900018K03Rik
Type: Gene
Species: Mouse
Chromosome: 4
NCBI: 214444
Homologene: 49533
Scaper
Name: S phase cyclin A-associated protein in the ER
Synonyms: D530014O03Rik, Zfp291
Type: Gene
Species: Mouse
Chromosome: 9
NCBI: 244891
VEGA: 9
Homologene: 32488
Apaf1
Name: apoptotic peptidase activating factor 1
Synonyms: 6230400I06Rik, Apaf1l
Type: Gene
Species: Mouse
Chromosome: 10
NCBI: 11783
HGNC: HGNC:576
Homologene: 7626
Lonp2
Name: lon peptidase 2, peroxisomal
Synonyms: 1300002A08Rik
Type: Gene
Species: Mouse
Chromosome: 8
NCBI: 66887
Homologene: 12050
Tonsl
Name: tonsoku-like, DNA repair protein
Synonyms: 2810439M11Rik, Nfkbil2
Type: Gene
Species: Mouse
Chromosome: 15
NCBI: 72749
HGNC: HGNC:7801
Homologene: 22754
Adgrb2
Name: adhesion G protein-coupled receptor B2
Synonyms: Bai2
Type: Gene
Species: Mouse
Chromosome: 4
NCBI: 230775
HGNC: HGNC:944
Homologene: 1288
Ecel1
Name: endothelin converting enzyme-like 1
Synonyms: XCE, DINE
Type: Gene
Species: Mouse
Chromosome: 1
NCBI: 13599
HGNC: HGNC:3147
Homologene: 3549
Exosc7
Name: exosome component 7
Synonyms: 2610002K22Rik
Type: Gene
Species: Mouse
Chromosome: 9
NCBI: 66446
Homologene: 8994
Magi3
Name: membrane associated guanylate kinase, WW and PDZ domain containing 3
Synonyms: 4732496O19Rik, 6530407C02Rik
Type: Gene
Species: Mouse
Chromosome: 3
NCBI: 99470
Homologene: 26431
Esco1
Name: establishment of sister chromatid cohesion N-acetyltransferase 1
Synonyms: A930014I12Rik
Type: Gene
Species: Mouse
Chromosome: 18
NCBI: 77805
Homologene: 62166
Galnt3
Name: polypeptide N-acetylgalactosaminyltransferase 3
Synonyms: ppGaNTase-T3
Type: Gene
Species: Mouse
Chromosome: 2
NCBI: 14425
HGNC: HGNC:4125
Homologene: 55827
Pde4d
Name: phosphodiesterase 4D, cAMP specific
Synonyms: dunce, Dpde3, 9630011N22Rik
Type: Gene
Species: Mouse
Chromosome: 13
NCBI: 238871
HGNC: HGNC:8783
Kcnq1
Name: potassium voltage-gated channel, subfamily Q, member 1
Synonyms: KVLQT1, Kcna9
Type: Gene
Species: Mouse
Chromosome: 7
NCBI: 16535
HGNC: HGNC:6294
Homologene: 85014
Ush2a
Name: usherin
Synonyms: MUSH2A, LOC269160, Ush2a, A930037M10Rik, A930011D15Rik, LOC381317, Ushrn
Type: Gene
Species: Mouse
Chromosome: 1
NCBI: 22283
Homologene: 66151
Drc7
Name: dynein regulatory complex subunit 7
Synonyms: Ccdc135, SRG-L, LOC330830
Type: Gene
Species: Mouse
Chromosome: 8
NCBI: 330830
Homologene: 12996
Dock10
Name: dedicator of cytokinesis 10
Synonyms: A630054M16Rik, Zizimin3, Jr5, Jr4, ZIZ3, 9330153B10Rik
Type: Gene
Species: Mouse
Chromosome: 1
NCBI: 210293
Homologene: 45952
Dnah9
Name: dynein, axonemal, heavy chain 9
Synonyms: Dnahc9, D11Ertd686e
Type: Gene
Species: Mouse
Chromosome: 11
NCBI: 237806
HGNC: HGNC:2953
Homologene: 20357
Slc36a2
Name: solute carrier family 36 (proton/amino acid symporter), member 2
Synonyms: Tramd1, PAT2, A530067G19Rik
Type: Gene
Species: Mouse
Chromosome: 11
NCBI: 246049
Homologene: 72100
Plcl1
Name: phospholipase C-like 1
Synonyms: C230017K02Rik, PLC-L, PRIP-1
Type: Gene
Species: Mouse
Chromosome: 1
NCBI: 227120
HGNC: HGNC:9063
Homologene: 38155
Pik3r5
Name: phosphoinositide-3-kinase regulatory subunit 5
Synonyms: p101, Foap2
Type: Gene
Species: Mouse
Chromosome: 11
NCBI: 320207
Homologene: 8627
Arpc1b
Name: actin related protein 2/3 complex, subunit 1B
Synonyms: L72, SOP2Hs, p41-ARC
Type: Gene
Species: Mouse
Chromosome: 5
NCBI: 11867
HGNC: HGNC:704
Homologene: 4179
Mansc4
Name: MANSC domain containing 4
Synonyms: Gm5887
Type: Gene
Species: Mouse
Chromosome: 6
NCBI: 545893
Homologene: 86837
Acer3
Name: alkaline ceramidase 3
Synonyms: 5430429L08Rik, Phca, 1110057L18Rik
Type: Gene
Species: Mouse
Chromosome: 7
NCBI: 66190
Homologene: 5410
Sfxn4
Name: sideroflexin 4
Type: Gene
Species: Mouse
Chromosome: 19
NCBI: 94281
Homologene: 14228
Plekhd1
Name: pleckstrin homology domain containing, family D (with coiled-coil domains) member 1
Synonyms: 3830431G21Rik
Type: Gene
Species: Mouse
Chromosome: 12
NCBI: 217682
Homologene: 15090
Zfp69
Name: zinc finger protein 69
Synonyms: LOC381549, Zfp63, KRAB2
Type: Gene
Species: Mouse
Chromosome: 4
NCBI: 381549
Homologene: 45693
Stra6l
Name: STRA6-like
Synonyms: 1300002K09Rik, Rbpr2
Type: Gene
Species: Mouse
Chromosome: 4
NCBI: 74152
Homologene: 81924
Or5d47
Name: olfactory receptor family 5 subfamily D member 47
Synonyms: Olfr74, GA_x6K02T2Q125-49458388-49457432, MOR174-4, mOR-EV
Type: Gene
Species: Mouse
Chromosome: 2
NCBI: 117005
Homologene: 74078
Cyp4a14
Name: cytochrome P450, family 4, subfamily a, polypeptide 14
Type: Gene
Species: Mouse
Chromosome: 4
NCBI: 13119
Homologene: 134697
Mill2
Name: MHC I like leukocyte 2
Type: Gene
Species: Mouse
Chromosome: 7
NCBI: 243864
Homologene: 88329
Anapc13
Name: anaphase promoting complex subunit 13
Synonyms: 1810004D07Rik
Type: Gene
Species: Mouse
Chromosome: 9
NCBI: 69010
Homologene: 32258
Hand2
Name: heart and neural crest derivatives expressed 2
Synonyms: Hed, bHLHa26, Thing2, Ehand2, dHAND
Type: Gene
Species: Mouse
Chromosome: 8
NCBI: 15111
HGNC: HGNC:4808
Homologene: 32092
Genetic Alterations
ENU-induced transitions at the following base pair locations (GRCm38):
  • C to T, chromosome 1 at 55,696,748 bp
  • T to C, chromosome 1 at 80,503,671 bp
  • A to G, chromosome 1 at 87,154,842 bp
  • C to A, chromosome 1 at 188,451,802 bp
  • A to G, chromosome 2 at 66,102,531 bp
  • G to A, chromosome 2 at 87,974,041 bp
  • A to G, chromosome 3 at 104,085,220 bp
  • G to A, chromosome 4 at 45,860,041 bp
  • T to C, chromosome 4 at 70,354,813 bp
  • T to A, chromosome 4 at 115,489,959 bp
  • T to C, chromosome 4 at 120,931,197 bp
  • T to C, chromosome 4 at 130,022,219 bp
  • A to T, chromosome 5 at 122,457,198 bp
  • A to T, chromosome 5 at 145,122,567 bp
  • A to T, chromosome 6 at 29,572,703 bp
  • A to T, chromosome 6 at 147,086,873 bp
  • T to A, chromosome 7 at 18,856,596 bp
  • A to T, chromosome 7 at 98,226,448 bp
  • C to T, chromosome 7 at 143,194,327 bp
  • C to A, chromosome 8 at 57,322,036 bp
  • A to G, chromosome 8 at 86,716,458 bp
  • T to C, chromosome 8 at 95,071,282 bp
  • A to G, chromosome 9 at 53,223,688 bp
  • A to T, chromosome 9 at 55,883,976 bp
  • T to C, chromosome 9 at 102,634,093 bp
  • A to G, chromosome 9 at 123,132,012 bp
  • T to C, chromosome 10 at 91,056,000 bp
  • A to T, chromosome 11 at 55,184,867 bp
  • T to C, chromosome 11 at 65,955,248 bp
  • G to A, chromosome 11 at 68,490,617 bp
  • T to C, chromosome 12 at 80,707,257 bp
  • A to G, chromosome 13 at 109,632,901 bp
  • A to G, chromosome 15 at 76,629,677 bp
  • T to A, chromosome 18 at 10,594,794 bp
  • A to G, chromosome 18 at 57,389,274 bp
  • T to C, chromosome 19 at 60,839,023 bp
Phenotype
G1 male heterozygous mice are viable and fertile. The G1 mice were not characterized prior to archiving.
Strain Development
C57BL/6J mice were injected with ENU, resulting in G0 mice that were bred back to C57BL/6J. Sperm was archived from G1 male mice that produced viable G2 mice.
Suggested Control Mice
Wild-type littermates or C57BL/6J mice
MMRRC Genetic QC Summary
The MMRRC Centers have developed a genetic QC pipeline using MiniMUGA array genotyping to provide additional information on strain backgrounds for MMRRC congenic and inbred strains. For more information on when data may be available, or to request genotyping for a strain of interest, please contact mmrrc@missouri.edu. Older strains may not have this information.
Donor
Bruce Beutler, M.D., University of Texas Southwestern Medical Center.
Primary Reference
MUTAGENETIX record for R6534 (G1). B. Beutler and colleagues, Center for the Genetics of Host Defense, UT Southwestern, Dallas, TX.

Colony and Husbandry Information

Colony Surveillance Program and Current Health Reports

Mice recovered from a cryo-archive will have health surveillance performed on recipient females. Health reports will be provided prior to shipment. If you require additional health status information, please email mmrrc@missouri.edu.
Coat Color
Black
MMRRC Breeding System
Random intra-strain mating
Breeding Scheme(s)
When maintaining a live colony, heterozygous mice may be bred together, bred with wild-type siblings, or bred with C57BL/6J inbred mice.
Overall Breeding Performance
Undetermined; during development, mating was done with heterozygous males; viability, fertility and other breeding statistics for heterozygous females and homozygotes are unverified, but presumably would be similar to the C57BL/6J background strain
Average litter size
Undetermined
Recommended wean age
3 weeks

Order Request Information

Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.

Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.

The donor or their institution limits the distribution to non-profit institutions only.

Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.

Click button to Request this one strain. (Use the MMRRC Catalog Search to request more than one strain.)
MMRRC Item # Description Distribution Fee / Unit (US $)
*Shipping & Handling not included*
Units Notes
044660-MU-RESUS Litter recovered from cryo-archive $5,475.00 / Non-Profit Litter Recovered litter4; additional fees for any special requests.
Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

1 The distribution fee covers the expense of rederiving mice from a live mouse; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

2 An aliquot contains a sufficient number of embryos (in one or more vials or straws and based on the transfer success rate of the MMRRC facility) to transfer into one to three recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.

3 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.

4 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.