Strain Name:
C57BL/6J-MtgxR6543Btlr/Mmmh
Stock Number:
044669-MU
Citation ID:
RRID:MMRRC_044669-MU
Other Names:
R6543 (G1)
Major Collection:

Strain Information

Ap4e1
Name: adaptor-related protein complex AP-4, epsilon 1
Synonyms: 2310033A20Rik
Type: Gene
Species: Mouse
Chromosome: 2
NCBI: 108011
HGNC: HGNC:573
Homologene: 22397
Itga8
Name: integrin alpha 8
Type: Gene
Species: Mouse
Chromosome: 2
NCBI: 241226
HGNC: HGNC:6144
Homologene: 37396
Atl3
Name: atlastin GTPase 3
Synonyms: 5730596K20Rik, 4633402C03Rik
Type: Gene
Species: Mouse
Chromosome: 19
NCBI: 109168
VEGA: 19
Homologene: 9149
Lims1
Name: LIM and senescent cell antigen-like domains 1
Synonyms: PINCH1, Lims1l, 2310016J22Rik, 4921524A02Rik, C430041B13Rik
Type: Gene
Species: Mouse
Chromosome: 10
NCBI: 110829
HGNC: HGNC:6616
Homologene: 68428
Mtor
Name: mechanistic target of rapamycin kinase
Synonyms: mechanistic target of rapamycin (serine/threonine kinase), Frap1, 2610315D21Rik, RAPT1, flat, FKBP-rapamycin-associated protein FRAP, RAFT1
Type: Gene
Species: Mouse
Chromosome: 4
NCBI: 56717
HGNC: HGNC:3942
Homologene: 3637
Ubr7
Name: ubiquitin protein ligase E3 component n-recognin 7 (putative)
Synonyms: 5730410I19Rik
Type: Gene
Species: Mouse
Chromosome: 12
NCBI: 66622
VEGA: 12
Homologene: 11998
Focad
Name: focadhesin
Synonyms: BC057079
Type: Gene
Species: Mouse
Chromosome: 4
NCBI: 230393
Homologene: 9842
Nfrkb
Name: nuclear factor related to kappa B binding protein
Synonyms: A530090G11Rik
Type: Gene
Species: Mouse
Chromosome: 9
NCBI: 235134
HGNC: HGNC:7802
Homologene: 4492
Trim66
Name: tripartite motif-containing 66
Synonyms: D7H11orf29, Tif1d
Type: Gene
Species: Mouse
Chromosome: 7
NCBI: 330627
Homologene: 28044
Borcs5
Name: BLOC-1 related complex subunit 5
Synonyms: Loh12cr1, 5830457J20Rik
Type: Gene
Species: Mouse
Chromosome: 6
NCBI: 67774
Homologene: 12188
Pgd
Name: phosphogluconate dehydrogenase
Synonyms: 0610042A05Rik
Type: Gene
Species: Mouse
Chromosome: 4
NCBI: 110208
HGNC: HGNC:8891
Homologene: 68268
Nbn
Name: nibrin
Synonyms: Nbs1
Type: Gene
Species: Mouse
Chromosome: 4
NCBI: 27354
HGNC: HGNC:7652
Homologene: 1858
Prokr2
Name: prokineticin receptor 2
Synonyms: Gpr73l1, EG-VEGRF2, B830005M06Rik, PKR2, Gpcr73l1
Type: Gene
Species: Mouse
Chromosome: 2
NCBI: 246313
Homologene: 16368
Nbeal2
Name: neurobeachin-like 2
Synonyms: 1110014F23Rik
Type: Gene
Species: Mouse
Chromosome: 9
NCBI: 235627
Homologene: 86422
Creld2
Name: cysteine-rich with EGF-like domains 2
Synonyms: 5730592L21Rik
Type: Gene
Species: Mouse
Chromosome: 15
NCBI: 76737
VEGA: 15
Homologene: 11526
Cuzd1
Name: CUB and zona pellucida-like domains 1
Synonyms: Itmap1, UO-44, UTCZP, ERG-1
Type: Gene
Species: Mouse
Chromosome: 7
NCBI: 16433
Homologene: 7389
Limk2
Name: LIM domain kinase 2
Synonyms: whe, Limk2b, Limk2a, A930024P04Rik, LIM kinase 2
Type: Gene
Species: Mouse
Chromosome: 11
NCBI: 16886
HGNC: HGNC:6614
Homologene: 55911
Pcnx3
Name: pecanex homolog 3
Synonyms: Pcnxl3
Type: Gene
Species: Mouse
Chromosome: 19
NCBI: 104401
Homologene: 17000
Sympk
Name: symplekin
Synonyms: 1500016F02Rik, 4632415H16Rik
Type: Gene
Species: Mouse
Chromosome: 7
NCBI: 68188
Homologene: 37969
N4bp1
Name: NEDD4 binding protein 1
Type: Gene
Species: Mouse
Chromosome: 8
NCBI: 80750
Homologene: 23703
Esyt3
Name: extended synaptotagmin-like protein 3
Synonyms: Fam62c, D9Ertd280e
Type: Gene
Species: Mouse
Chromosome: 9
NCBI: 272636
Homologene: 18626
Ttn
Name: titin
Synonyms: connectin, 1100001C23Rik, D330041I19Rik, D830007G01Rik, 2310074I15Rik, 2310036G12Rik, mdm, 2310057K23Rik, shru, L56
Type: Gene
Species: Mouse
Chromosome: 2
NCBI: 22138
Homologene: 130650
Il20ra
Name: interleukin 20 receptor, alpha
Type: Gene
Species: Mouse
Chromosome: 10
NCBI: 237313
VEGA: 10
HGNC: HGNC:6003
Homologene: 8685
Rasef
Name: RAS and EF hand domain containing
Synonyms: RAB45
Type: Gene
Species: Mouse
Chromosome: 4
NCBI: 242505
Homologene: 28424
Tnr
Name: tenascin R
Synonyms: janusin, J1-tenascin, TN-R, restrictin
Type: Gene
Species: Mouse
Chromosome: 1
NCBI: 21960
Homologene: 124416
Slco1a6
Name: solute carrier organic anion transporter family, member 1a6
Synonyms: organic anion-transporting polypeptide, Slc21a13, Oatp-5, 4930422F19Rik
Type: Gene
Species: Mouse
Chromosome: 6
NCBI: 28254
Homologene: 23416
Dclk1
Name: doublecortin-like kinase 1
Synonyms: 2810480F11Rik, CPG16, DCLK, Click-I, Dcl, Dcamkl1, 1700113D08Rik
Type: Gene
Species: Mouse
Chromosome: 3
NCBI: 13175
HGNC: HGNC:2700
Homologene: 130530
Man1a
Name: mannosidase 1, alpha
Synonyms: PCR1, mannosyl-oligosaccharide alpha-1,2-mannosidase
Type: Gene
Species: Mouse
Chromosome: 10
NCBI: 17155
HGNC: HGNC:6821
Homologene: 4316
Arhgef11
Name: Rho guanine nucleotide exchange factor 11
Synonyms: Prg, PDZ-RhoGEF
Type: Gene
Species: Mouse
Chromosome: 3
NCBI: 213498
Homologene: 11409
Topaz1
Name: testis and ovary specific PAZ domain containing 1
Synonyms: Gm9524
Type: Gene
Species: Mouse
Chromosome: 9
NCBI: 671232
VEGA: 9
Homologene: 19835
Il6ra
Name: interleukin 6 receptor, alpha
Synonyms: Il6r, IL-6 receptor alpha chain, IL-6R, CD126
Type: Gene
Species: Mouse
Chromosome: 3
NCBI: 16194
HGNC: HGNC:6019
Homologene: 474
Pxmp4
Name: peroxisomal membrane protein 4
Synonyms: 3010018P03Rik
Type: Gene
Species: Mouse
Chromosome: 2
NCBI: 59038
Homologene: 5237
Sipa1l2
Name: signal-induced proliferation-associated 1 like 2
Type: Gene
Species: Mouse
Chromosome: 8
NCBI: 244668
Homologene: 18956
Grip1
Name: glutamate receptor interacting protein 1
Synonyms: eb, 4931400F03Rik
Type: Gene
Species: Mouse
Chromosome: 10
NCBI: 74053
Homologene: 12938
Cplane2
Name: ciliogenesis and planar polarity effector 2
Synonyms: Rsg1, 6330545A04Rik, b2b2804Clo, b2b2827Clo, LOC279260
Type: Gene
Species: Mouse
Chromosome: 4
NCBI: 76166
Homologene: 12793
Scpppq1
Name: secretory calcium-binding phosphoprotein proline-glutamine rich 1
Synonyms: Gm17660
Type: Gene
Species: Mouse
Chromosome: 5
NCBI: 100271704
Homologene: 130040
Slfn5
Name: schlafen 5
Type: Gene
Species: Mouse
Chromosome: 11
NCBI: 327978
Homologene: 18839
Slc26a8
Name: solute carrier family 26, member 8
Type: Gene
Species: Mouse
Chromosome: 17
NCBI: 224661
Homologene: 27095
Scrt2
Name: scratch family zinc finger 2
Type: Gene
Species: Mouse
Chromosome: 2
NCBI: 545474
Homologene: 99867
Or6c3b
Name: olfactory receptor family 6 subfamily C member 3B
Synonyms: GA_x6K02T2PULF-11370664-11369738, MOR111-3, Olfr803
Type: Gene
Species: Mouse
Chromosome: 10
NCBI: 258547
Homologene: 105153
Trav6-3
Name: T cell receptor alpha variable 6-3
Type: Gene
Species: Mouse
Chromosome: 14
NCBI: 328483
Igkv4-91
Name: immunoglobulin kappa chain variable 4-91
Synonyms: ENSMUSG00000056850, Gm10879
Type: Gene
Species: Mouse
Chromosome: 6
NCBI: 434033
Msantd5f4
Name: Myb/SANT DNA binding domain containing 5 family member 4
Synonyms: Gm11236
Type: Gene
Species: Mouse
Chromosome: 4
NCBI: 100862015
Homologene: 128423
Genetic Alterations
ENU-induced transitions at the following base pair locations (GRCm38):
  • A to G, chromosome 1 at 159,924,107 bp
  • C to T, chromosome 2 at 12,301,644 bp
  • T to C, chromosome 2 at 76,768,811 bp
  • T to C, chromosome 2 at 127,066,605 bp
  • T to A, chromosome 2 at 132,373,899 bp
  • C to T, chromosome 2 at 152,093,143 bp
  • C to A, chromosome 2 at 154,588,059 bp
  • C to T, chromosome 3 at 55,500,131 bp
  • A to G, chromosome 3 at 87,733,408 bp
  • A to G, chromosome 3 at 89,876,863 bp
  • T to C, chromosome 4 at 15,986,605 bp
  • A to G, chromosome 4 at 73,638,978 bp
  • C to T, chromosome 4 at 73,780,519 bp
  • T to C, chromosome 4 at 88,279,256 bp
  • T to G, chromosome 4 at 141,217,288 bp
  • T to A, chromosome 4 at 148,545,596 bp
  • C to T, chromosome 4 at 149,160,752 bp
  • T to C, chromosome 5 at 104,074,875 bp
  • A to T, chromosome 6 at 68,768,598 bp
  • C to T, chromosome 6 at 134,710,180 bp
  • T to C, chromosome 6 at 142,133,146 bp
  • A to T, chromosome 7 at 19,036,830 bp
  • A to G, chromosome 7 at 109,475,879 bp
  • A to G, chromosome 7 at 131,309,768 bp
  • G to A, chromosome 8 at 86,861,906 bp
  • T to C, chromosome 8 at 125,450,362 bp
  • C to T, chromosome 9 at 31,400,985 bp
  • T to C, chromosome 9 at 99,338,772 bp
  • A to T, chromosome 9 at 110,644,458 bp
  • A to G, chromosome 9 at 122,748,535 bp
  • G to A, chromosome 10 at 19,749,323 bp
  • C to A, chromosome 10 at 53,934,981 bp
  • T to A, chromosome 10 at 58,412,451 bp
  • T to C, chromosome 10 at 119,985,594 bp
  • C to A, chromosome 10 at 129,691,990 bp
  • T to C, chromosome 11 at 3,350,682 bp
  • T to A, chromosome 11 at 82,958,666 bp
  • A to G, chromosome 12 at 102,768,235 bp
  • T to C, chromosome 14 at 53,428,762 bp
  • A to G, chromosome 15 at 88,825,278 bp
  • T to C, chromosome 17 at 28,638,401 bp
  • C to T, chromosome 19 at 5,665,247 bp
  • T to A, chromosome 19 at 7,510,098 bp
Phenotype
G1 male heterozygous mice are viable and fertile. The G1 mice were not characterized prior to archiving.
Strain Development
C57BL/6J mice were injected with ENU, resulting in G0 mice that were bred back to C57BL/6J. Sperm was archived from G1 male mice that produced viable G2 mice.
Suggested Control Mice
Wild-type littermates or C57BL/6J mice
MMRRC Genetic QC Summary
The MMRRC Centers have developed a genetic QC pipeline using MiniMUGA array genotyping to provide additional information on strain backgrounds for MMRRC congenic and inbred strains. For more information on when data may be available, or to request genotyping for a strain of interest, please contact mmrrc@missouri.edu. Older strains may not have this information.
Donor
Bruce Beutler, M.D., University of Texas Southwestern Medical Center.
Primary Reference
MUTAGENETIX record for R6543 (G1). B. Beutler and colleagues, Center for the Genetics of Host Defense, UT Southwestern, Dallas, TX.

Colony and Husbandry Information

Colony Surveillance Program and Current Health Reports

Mice recovered from a cryo-archive will have health surveillance performed on recipient females. Health reports will be provided prior to shipment. If you require additional health status information, please email mmrrc@missouri.edu.
Coat Color
Black
MMRRC Breeding System
Random intra-strain mating
Breeding Scheme(s)
When maintaining a live colony, heterozygous mice may be bred together, bred with wild-type siblings, or bred with C57BL/6J inbred mice.
Overall Breeding Performance
Undetermined; during development, mating was done with heterozygous males; viability, fertility and other breeding statistics for heterozygous females and homozygotes are unverified, but presumably would be similar to the C57BL/6J background strain
Average litter size
Undetermined
Recommended wean age
3 weeks

Order Request Information

Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.

Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.

The donor or their institution limits the distribution to non-profit institutions only.

Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.

Click button to Request this one strain. (Use the MMRRC Catalog Search to request more than one strain.)
MMRRC Item # Description Distribution Fee / Unit (US $)
*Shipping & Handling not included*
Units Notes
044669-MU-RESUS Litter recovered from cryo-archive $5,475.00 / Non-Profit Litter Recovered litter4; additional fees for any special requests.
Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

1 The distribution fee covers the expense of rederiving mice from a live mouse; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

2 An aliquot contains a sufficient number of embryos (in one or more vials or straws and based on the transfer success rate of the MMRRC facility) to transfer into one to three recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.

3 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.

4 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.