Strain Name:
C57BL/6J-MtgxR6544Btlr/Mmmh
Stock Number:
044670-MU
Citation ID:
RRID:MMRRC_044670-MU
Other Names:
R6544 (G1)
Major Collection:

Strain Information

Sparcl1
Name: SPARC-like 1
Synonyms: Sc1, hevin, mast9, Ecm2
Type: Gene
Species: Mouse
Chromosome: 5
NCBI: 13602
Homologene: 3438
Ank2
Name: ankyrin 2, brain
Synonyms: ankyrin B, Ank-2, Ankyrin-2, Ankyrin-B, Gm4392
Type: Gene
Species: Mouse
Chromosome: 3
NCBI: 109676
HGNC: HGNC:493
Mok
Name: MOK protein kinase
Synonyms: MOK, MAPK/MAK/MRK/ overlapping kinase, Rage, Stk30
Type: Gene
Species: Mouse
Chromosome: 12
NCBI: 26448
HGNC: HGNC:9833
Homologene: 8062
Rorb
Name: RAR-related orphan receptor beta
Synonyms: RZR-beta, Nr1f2, Rorbeta, hstp
Type: Gene
Species: Mouse
Chromosome: 19
NCBI: 225998
Homologene: 38250
Gigyf1
Name: GRB10 interacting GYF protein 1
Synonyms: Perq1
Type: Gene
Species: Mouse
Chromosome: 5
NCBI: 57330
HGNC: HGNC:9126
Homologene: 57145
Pspc1
Name: paraspeckle protein 1
Synonyms: 5730470C09Rik, PSP1
Type: Gene
Species: Mouse
Chromosome: 14
NCBI: 66645
Homologene: 10108
Pip5k1c
Name: phosphatidylinositol-4-phosphate 5-kinase, type 1 gamma
Synonyms: PIP5KIgamma
Type: Gene
Species: Mouse
Chromosome: 10
NCBI: 18717
HGNC: HGNC:8996
Homologene: 69032
Actr2
Name: actin related protein 2
Synonyms: 4921510D23Rik, D6Ertd746e, Arp2
Type: Gene
Species: Mouse
Chromosome: 11
NCBI: 66713
HGNC: HGNC:169
Homologene: 4181
Lmtk2
Name: lemur tyrosine kinase 2
Synonyms: 2900041G10Rik, BREK, KPI-2, cprk, KPI2, A330101P12Rik, AATYK2
Type: Gene
Species: Mouse
Chromosome: 5
NCBI: 231876
Homologene: 8948
Cog7
Name: component of oligomeric golgi complex 7
Synonyms: 5630400E24Rik
Type: Gene
Species: Mouse
Chromosome: 7
NCBI: 233824
Homologene: 33431
Ark2c
Name: arkadia (RNF111) C-terminal like ring finger ubiquitin ligase 2C
Synonyms: LOC225743, 2900024M11Rik, G630064H08Rik, Ark2c, Rnf165
Type: Gene
Species: Mouse
Chromosome: 18
NCBI: 225743
Homologene: 79757
Angptl3
Name: angiopoietin-like 3
Synonyms: hypl
Type: Gene
Species: Mouse
Chromosome: 4
NCBI: 30924
HGNC: HGNC:491
Homologene: 8499
Cadm3
Name: cell adhesion molecule 3
Synonyms: Tsll1, BIgR, Necl1, Necl-1, SynCAM3, Igsf4b
Type: Gene
Species: Mouse
Chromosome: 1
NCBI: 94332
Homologene: 10919
Grik4
Name: glutamate receptor, ionotropic, kainate 4
Synonyms: KA1, 6330551K01Rik, GluRgamma1, KA-1
Type: Gene
Species: Mouse
Chromosome: 9
NCBI: 110637
HGNC: HGNC:4582
Homologene: 81829
Sox17
Name: SRY (sex determining region Y)-box 17
Type: Gene
Species: Mouse
Chromosome: 1
NCBI: 20671
Homologene: 7948
Tns2
Name: tensin 2
Synonyms: nep, nph, Tenc1
Type: Gene
Species: Mouse
Chromosome: 15
NCBI: 209039
VEGA: 15
Homologene: 37077
Ttn
Name: titin
Synonyms: connectin, L56, mdm, 1100001C23Rik, D830007G01Rik, 2310036G12Rik, 2310074I15Rik, 2310057K23Rik, D330041I19Rik, shru
Type: Gene
Species: Mouse
Chromosome: 2
NCBI: 22138
Homologene: 130650
Dchs1
Name: dachsous cadherin related 1
Synonyms: C130033F22Rik, 3110041P15Rik
Type: Gene
Species: Mouse
Chromosome: 7
NCBI: 233651
Homologene: 2771
Ptprq
Name: protein tyrosine phosphatase receptor type Q
Type: Gene
Species: Mouse
Chromosome: 10
NCBI: 237523
HGNC: HGNC:9679
Homologene: 83557
Naip5
Name: NLR family, apoptosis inhibitory protein 5
Synonyms: Naip-rs3, Birc1e, Lgn1
Type: Gene
Species: Mouse
Chromosome: 13
NCBI: 17951
HGNC: HGNC:7634
Homologene: 113589
Scn7a
Name: sodium channel, voltage-gated, type VII, alpha
Synonyms: Nav2.3, NaG, Nav2, 1110034K09Rik, Scn6a, Nax
Type: Gene
Species: Mouse
Chromosome: 2
NCBI: 20272
Homologene: 55706
Ahnak2
Name: AHNAK nucleoprotein 2
Synonyms: LOC382643
Type: Gene
Species: Mouse
Chromosome: 12
NCBI: 100041194
Homologene: 131081
Mprip
Name: myosin phosphatase Rho interacting protein
Synonyms: RIP3, p116Rip, p116 Rho interacting protein, Rhoip3, Gm34094
Type: Gene
Species: Mouse
Chromosome: 11
NCBI: 26936
Homologene: 9034
Fbxo47
Name: F-box protein 47
Synonyms: LOC380724, 2900052P03Rik
Type: Gene
Species: Mouse
Chromosome: 11
NCBI: 72973
Homologene: 47677
Neu2
Name: neuraminidase 2
Synonyms: brain sialidase, cystolic sialidase, MSS, MBS, MTS
Type: Gene
Species: Mouse
Chromosome: 1
NCBI: 23956
HGNC: HGNC:7759
Homologene: 3927
Frmpd1
Name: FERM and PDZ domain containing 1
Type: Gene
Species: Mouse
Chromosome: 4
NCBI: 666060
Homologene: 8939
Slco1c1
Name: solute carrier organic anion transporter family, member 1c1
Synonyms: OATP-F, Slc21a14
Type: Gene
Species: Mouse
Chromosome: 6
NCBI: 58807
Homologene: 23008
Gucy2e
Name: guanylate cyclase 2e
Synonyms: ROS-GC1, GC-E, GC1
Type: Gene
Species: Mouse
Chromosome: 11
NCBI: 14919
HGNC: HGNC:4689
Homologene: 55442
Zc3h15
Name: zinc finger CCCH-type containing 15
Synonyms: FM22, Ierepo4, 1810012H02Rik, 1700006A17Rik, 2610312B22Rik
Type: Gene
Species: Mouse
Chromosome: 2
NCBI: 69082
Homologene: 10216
Or1ak2
Name: olfactory receptor family 1 subfamily AK member 2
Synonyms: GA_x6K02T2NLDC-33631647-33632594, MOR134-1, Olfr356
Type: Gene
Species: Mouse
Chromosome: 2
NCBI: 258617
Homologene: 85948
Lats1
Name: large tumor suppressor
Type: Gene
Species: Mouse
Chromosome: 10
NCBI: 16798
VEGA: 10
HGNC: HGNC:6514
Homologene: 55843
Tpte
Name: transmembrane phosphatase with tensin homology
Synonyms: Pten2, Vsp
Type: Gene
Species: Mouse
Chromosome: 8
NCBI: 234129
Homologene: 50411
Smarca2
Name: SWI/SNF related, matrix associated, actin dependent regulator of chromatin, subfamily a, member 2
Synonyms: brm, Snf2l2, 2610209L14Rik
Type: Gene
Species: Mouse
Chromosome: 19
NCBI: 67155
Homologene: 2308
Adam26b
Name: a disintegrin and metallopeptidase domain 26B
Type: Gene
Species: Mouse
Chromosome: 8
NCBI: 382007
Homologene: 128363
Zfp777
Name: zinc finger protein 777
Synonyms: 2500002G23Rik
Type: Gene
Species: Mouse
Chromosome: 6
NCBI: 72306
Homologene: 56721
Zfp455
Name: zinc finger protein 455
Synonyms: Rslcan-10, 3732412P20Rik
Type: Gene
Species: Mouse
Chromosome: 13
NCBI: 218311
Homologene: 110878
Or5l13
Name: olfactory receptor family 5 subfamily L member 13
Synonyms: GA_x6K02T2Q125-49433499-49432537, MOR174-3, Olfr1156
Type: Gene
Species: Mouse
Chromosome: 2
NCBI: 258814
HGNC: HGNC:8351
Homologene: 72031
Ahcyl
Name: adenosylhomocysteinase like
Synonyms: Gm4737
Type: Gene
Species: Mouse
Chromosome: 16
NCBI: 11615
VEGA: 16
HGNC: HGNC:343
Plch1
Name: phospholipase C, eta 1
Synonyms: PLCeta1, Plcl3
Type: Gene
Species: Mouse
Chromosome: 3
NCBI: 269437
Homologene: 88833
Serpine2
Name: serine (or cysteine) peptidase inhibitor, clade E, member 2
Synonyms: nexin, PN-1, PI7, protease nexin 1, Spi4, B230326M24Rik
Type: Gene
Species: Mouse
Chromosome: 1
NCBI: 20720
HGNC: HGNC:8951
Homologene: 21247
Hectd2
Name: HECT domain E3 ubiquitin protein ligase 2
Synonyms: A630025O09Rik
Type: Gene
Species: Mouse
Chromosome: 19
NCBI: 226098
Homologene: 6280
Or5an1
Name: olfactory receptor family 5 subfamily AN member 1
Synonyms: GA_x6K02T2RE5P-2618516-2619454, GA_x6K02T2RE5P-2610001-2610414, MOR214-4, MOR214-4, Olfr1433, Olfr1434
Type: Gene
Species: Mouse
Chromosome: 19
NCBI: 258680
Homologene: 45029
Or5as1
Name: olfactory receptor family 5 subfamily AS member 1
Synonyms: GA_x6K02T2Q125-48635468-48634530, MOR181-2, Olfr1111
Type: Gene
Species: Mouse
Chromosome: 2
NCBI: 258586
Homologene: 74238
Gprin1
Name: G protein-regulated inducer of neurite outgrowth 1
Synonyms: Z16, GRIN1
Type: Gene
Species: Mouse
Chromosome: 13
NCBI: 26913
Homologene: 8072
Map10
Name: microtubule-associated protein 10
Synonyms: 4933403G14Rik
Type: Gene
Species: Mouse
Chromosome: 8
NCBI: 74393
Homologene: 10416
Lactbl1
Name: lactamase, beta-like 1
Type: Gene
Species: Mouse
Chromosome: 4
NCBI: 242707
Homologene: 82492
Tdpoz2
Name: TD and POZ domain containing 2
Type: Gene
Species: Mouse
Chromosome: 3
NCBI: 399673
Homologene: 133956
Wdr97
Name: WD repeat domain 97
Synonyms: Gm35339
Type: Gene
Species: Mouse
Chromosome: 15
NCBI: 102638882
Homologene: 131539
A030005K14Rik
Name: RIKEN cDNA A030005K14 gene
Type: Gene
Species: Mouse
Chromosome: 1
Genetic Alterations
ENU-induced transitions at the following base pair locations (GRCm38):
  • G to T, chromosome 1 at 4,492,432 bp
  • C to T, chromosome 1 at 79,803,130 bp
  • T to A, chromosome 1 at 83,058,957 bp
  • T to C, chromosome 1 at 87,596,742 bp
  • A to G, chromosome 1 at 173,367,411 bp
  • T to A, chromosome 2 at 36,937,527 bp
  • A to T, chromosome 2 at 66,684,100 bp
  • A to T, chromosome 2 at 76,969,159 bp
  • G to A, chromosome 2 at 83,661,148 bp
  • T to A, chromosome 2 at 87,149,863 bp
  • T to A, chromosome 2 at 87,949,991 bp
  • T to C, chromosome 3 at 63,850,978 bp
  • T to C, chromosome 3 at 93,651,960 bp
  • T to C, chromosome 3 at 126,933,222 bp
  • A to T, chromosome 4 at 45,279,024 bp
  • T to C, chromosome 4 at 99,031,438 bp
  • A to T, chromosome 4 at 136,632,989 bp
  • A to T, chromosome 5 at 104,092,444 bp
  • T to A, chromosome 5 at 137,525,059 bp
  • A to G, chromosome 5 at 144,173,806 bp
  • A to T, chromosome 6 at 48,044,485 bp
  • A to G, chromosome 6 at 141,531,444 bp
  • T to A, chromosome 7 at 105,758,178 bp
  • C to T, chromosome 7 at 121,935,743 bp
  • G to T, chromosome 8 at 22,315,105 bp
  • T to C, chromosome 8 at 43,521,781 bp
  • T to C, chromosome 8 at 125,671,374 bp
  • A to T, chromosome 9 at 42,547,728 bp
  • T to A, chromosome 10 at 7,701,670 bp
  • T to A, chromosome 10 at 81,308,996 bp
  • T to C, chromosome 10 at 107,608,241 bp
  • A to G, chromosome 11 at 20,100,933 bp
  • G to A, chromosome 11 at 59,757,726 bp
  • A to G, chromosome 11 at 69,235,657 bp
  • G to A, chromosome 11 at 97,856,263 bp
  • A to G, chromosome 12 at 110,810,755 bp
  • A to G, chromosome 12 at 112,780,652 bp
  • G to A, chromosome 13 at 54,740,311 bp
  • C to A, chromosome 13 at 67,207,057 bp
  • C to A, chromosome 13 at 100,223,144 bp
  • T to C, chromosome 14 at 56,764,203 bp
  • T to C, chromosome 15 at 76,358,278 bp
  • A to C, chromosome 15 at 102,113,834 bp
  • A to C, chromosome 16 at 46,154,784 bp
  • T to A, chromosome 18 at 77,563,235 bp
  • T to C, chromosome 19 at 12,283,155 bp
  • G to T, chromosome 19 at 18,952,250 bp
  • T to A, chromosome 19 at 26,630,931 bp
  • C to T, chromosome 19 at 36,612,328 bp
Phenotype
G1 male heterozygous mice are viable and fertile. The G1 mice were not characterized prior to archiving.
Strain Development
C57BL/6J mice were injected with ENU, resulting in G0 mice that were bred back to C57BL/6J. Sperm was archived from G1 male mice that produced viable G2 mice.
Suggested Control Mice
Wild-type littermates or C57BL/6J mice
MMRRC Genetic QC Summary
The MMRRC Centers have developed a genetic QC pipeline using MiniMUGA array genotyping to provide additional information on strain backgrounds for MMRRC congenic and inbred strains. For more information on when data may be available, or to request genotyping for a strain of interest, please contact mmrrc@missouri.edu. Older strains may not have this information.
Donor
Bruce Beutler, M.D., University of Texas Southwestern Medical Center.
Primary Reference
MUTAGENETIX record for R6544 (G1). B. Beutler and colleagues, Center for the Genetics of Host Defense, UT Southwestern, Dallas, TX.

Colony and Husbandry Information

Colony Surveillance Program and Current Health Reports

Mice recovered from a cryo-archive will have health surveillance performed on recipient females. Health reports will be provided prior to shipment. If you require additional health status information, please email mmrrc@missouri.edu.
Coat Color
Black
MMRRC Breeding System
Random intra-strain mating
Breeding Scheme(s)
When maintaining a live colony, heterozygous mice may be bred together, bred with wild-type siblings, or bred with C57BL/6J inbred mice.
Overall Breeding Performance
Undetermined; during development, mating was done with heterozygous males; viability, fertility and other breeding statistics for heterozygous females and homozygotes are unverified, but presumably would be similar to the C57BL/6J background strain
Average litter size
Undetermined
Recommended wean age
3 weeks

Order Request Information

Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.

Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.

The donor or their institution limits the distribution to non-profit institutions only.

Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.

Click button to Request this one strain. (Use the MMRRC Catalog Search to request more than one strain.)
MMRRC Item # Description Distribution Fee / Unit (US $)
*Shipping & Handling not included*
Units Notes
044670-MU-RESUS Litter recovered from cryo-archive $5,475.00 / Non-Profit Litter Recovered litter4; additional fees for any special requests.
Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

1 The distribution fee covers the expense of rederiving mice from a live mouse; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

2 An aliquot contains a sufficient number of embryos (in one or more vials or straws and based on the transfer success rate of the MMRRC facility) to transfer into one to three recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.

3 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.

4 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.