Loading Mouse GIF
Loading...

Strain Name:
C57BL/6J-MtgxR6551Btlr/Mmmh
Stock Number:
044676-MU
Citation ID:
RRID:MMRRC_044676-MU
Other Names:
R6551 (G1)
Major Collection:

Strain Information

Rapgef2
Name: Rap guanine nucleotide exchange factor (GEF) 2
Synonyms: 5830453M24Rik, Pdzgef1, RA-GEF-1, CNRasGEF, nRapGEP
Type: Gene
Species: Mouse
Chromosome: 3
NCBI: 76089
Homologene: 35477
Pik3c2a
Name: phosphatidylinositol-4-phosphate 3-kinase catalytic subunit type 2 alpha
Synonyms: PI3KC2
Type: Gene
Species: Mouse
Chromosome: 7
NCBI: 18704
HGNC: HGNC:8971
Homologene: 20581
Fbxo31
Name: F-box protein 31
Synonyms: 2310046N15Rik, Fbx14, Fbxo14, 1110003O08Rik
Type: Gene
Species: Mouse
Chromosome: 8
NCBI: 76454
Homologene: 11684
Brwd1
Name: bromodomain and WD repeat domain containing 1
Synonyms: 5330419I02Rik, D530019K20Rik, G1-403-16, Wdr9, repro5
Type: Gene
Species: Mouse
Chromosome: 16
NCBI: 93871
Homologene: 23130
Unk
Name: unkempt family zinc finger
Synonyms: B230379M23Rik, Zc3h5
Type: Gene
Species: Mouse
Chromosome: 11
NCBI: 217331
Homologene: 18194
Nol9
Name: nucleolar protein 9
Synonyms: 6030462G04Rik, 4632412I24Rik
Type: Gene
Species: Mouse
Chromosome: 4
NCBI: 74035
Homologene: 32589
Cttnbp2nl
Name: CTTNBP2 N-terminal like
Type: Gene
Species: Mouse
Chromosome: 3
NCBI: 80281
Homologene: 36371
Genetic Alterations
ENU-induced transitions at the following base pair locations (GRCm38):
  • A to T, chromosome 1 at 65,048,637 bp
  • A to T, chromosome 1 at 193,174,523 bp
  • A to G, chromosome 2 at 85,041,106 bp
  • T to C, chromosome 2 at 87,846,661 bp
  • T to A, chromosome 2 at 119,571,813 bp
  • T to A, chromosome 2 at 126,733,182 bp
  • T to C, chromosome 2 at 155,551,208 bp
  • G to A, chromosome 2 at 164,909,365 bp
  • A to T, chromosome 3 at 79,215,035 bp
  • A to T, chromosome 3 at 105,005,117 bp
  • C to A, chromosome 4 at 101,193,843 bp
  • A to G, chromosome 4 at 129,692,103 bp
  • T to C, chromosome 4 at 133,613,649 bp
  • T to G, chromosome 4 at 152,022,221 bp
  • G to A, chromosome 4 at 152,051,868 bp
  • T to C, chromosome 4 at 152,128,766 bp
  • G to T, chromosome 4 at 155,785,692 bp
  • C to A, chromosome 5 at 38,233,272 bp
  • G to A, chromosome 6 at 71,510,365 bp
  • T to C, chromosome 6 at 115,012,181 bp
  • A to T, chromosome 6 at 130,275,755 bp
  • A to G, chromosome 6 at 135,733,344 bp
  • G to A, chromosome 7 at 19,201,163 bp
  • A to T, chromosome 7 at 116,417,496 bp
  • G to A, chromosome 8 at 27,156,484 bp
  • A to C, chromosome 8 at 121,564,704 bp
  • A to C, chromosome 9 at 18,562,562 bp
  • C to A, chromosome 9 at 39,572,560 bp
  • T to C, chromosome 9 at 40,771,277 bp
  • G to T, chromosome 11 at 49,563,989 bp
  • A to T, chromosome 11 at 58,732,757 bp
  • T to C, chromosome 11 at 73,940,303 bp
  • G to A, chromosome 11 at 75,906,546 bp
  • A to C, chromosome 11 at 84,521,913 bp
  • T to C, chromosome 11 at 116,050,724 bp
  • C to A, chromosome 13 at 84,222,418 bp
  • T to C, chromosome 16 at 95,993,962 bp
  • A to G, chromosome 17 at 12,905,569 bp
  • A to G, chromosome 17 at 34,599,468 bp
  • G to A, chromosome 17 at 37,405,905 bp
  • T to A, chromosome 17 at 56,998,945 bp
  • A to C, chromosome 17 at 80,204,219 bp
  • C to A, chromosome 18 at 20,539,911 bp
Phenotype
G1 male heterozygous mice are viable and fertile. The G1 mice were not characterized prior to archiving.
Strain GQC Summary
Gene Specific Genotyping:

To request gene-specific and other genotyping services for a strain, please contact the distribution MMRRC Center for more information.

Background Genetic Quality:

The MMRRC has developed a Genetic Quality Control pipeline using the MiniMUGA array to provide additional information to identify and validate genetic backgrounds of MMRRC strains. For more information on whether genetic background data is available, please contact MMRRC_GeneticQC@med.unc.edu. Note: that MiniMUGA genetic background data is not available on all strains, but can be ordered if desired.

Suggested Control Mice
Littermates of all relevant genotypes.
Donor
Bruce Beutler, M.D., University of Texas Southwestern Medical Center.
Primary Reference
MUTAGENETIX record for R6551 (G1). B. Beutler and colleagues, Center for the Genetics of Host Defense, UT Southwestern, Dallas, TX.
Strain Development
C57BL/6J mice were injected with ENU, resulting in G0 mice that were bred back to C57BL/6J. Sperm was archived from G1 male mice that produced viable G2 mice.


Disclaimer: If MMRRC Strain Genetic Quality Control (GQC; based on MiniMUGA genotyping and analysis) has been completed for this strain, the information might differ from the genetic background information provided by the submitter. MiniMUGA genetic analysis is done on a strain’s tissue samples taken when archived by or ordered from the assigned MMRRC Center.

Colony and Husbandry Information

Colony Surveillance Program and Current Health Reports

Mice recovered from a cryo-archive will have health surveillance performed on recipient females. Health reports will be provided prior to shipment. If you require additional health status information, please email mmrrc@missouri.edu.
Coat Color
Black
MMRRC Breeding System
Random intra-strain mating
Breeding Scheme(s)
When maintaining a live colony, heterozygous mice may be bred together, bred with wild-type siblings, or bred with C57BL/6J inbred mice.
Overall Breeding Performance
Undetermined; during development, mating was done with heterozygous males; viability, fertility and other breeding statistics for heterozygous females and homozygotes are unverified, but presumably would be similar to the C57BL/6J background strain
Average litter size
Undetermined
Recommended wean age
3 weeks

Order Information

Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.

Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.

The donor or their institution limits the distribution to non-profit institutions only.

Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.

Click button to Request this one strain. (Use the MMRRC Catalog Search to request more than one strain.)
MMRRC Item # Description Distribution Fee / Unit (US $)
*Shipping & Handling not included*
Units Notes
044676-MU-RESUS Litter recovered from cryo-archive $5,475.00 / Non-Profit Litter Recovered litter4; additional fees for any special requests.
Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

1 The distribution fee covers the expense of rederiving mice from a live mouse; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

2 An aliquot contains a sufficient number of embryos (in one or more vials or straws and based on the transfer success rate of the MMRRC facility) to transfer into one to three recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.

3 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.

4 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.