Strain Name:
C57BL/6J-MtgxR6565Btlr/Mmmh
Stock Number:
044689-MU
Citation ID:
RRID:MMRRC_044689-MU
Other Names:
R6565 (G1)
Major Collection:

Strain Information

Kit
Name: KIT proto-oncogene receptor tyrosine kinase
Synonyms: SCO1, Steel Factor Receptor, belly-spot, SCO5, Tr-kit, Dominant white spotting, Gsfsco1, SOW3, c-KIT, CD117, Gsfsco5, Gsfsow3
Type: Gene
Species: Mouse
Chromosome: 5
NCBI: 16590
HGNC: HGNC:6342
Homologene: 187
Adgrb2
Name: adhesion G protein-coupled receptor B2
Synonyms: Bai2
Type: Gene
Species: Mouse
Chromosome: 4
NCBI: 230775
HGNC: HGNC:944
Homologene: 1288
Cadm3
Name: cell adhesion molecule 3
Synonyms: Tsll1, SynCAM3, BIgR, Necl-1, Igsf4b, Necl1
Type: Gene
Species: Mouse
Chromosome: 1
NCBI: 94332
Homologene: 10919
Mia3
Name: MIA SH3 domain ER export factor 3
Synonyms: B230399H06Rik, 9130229H14Rik, LOC385255, Tango, A930039G15Rik
Type: Gene
Species: Mouse
Chromosome: 1
NCBI: 338366
Homologene: 85165
Acvr1
Name: activin A receptor, type 1
Synonyms: Acvrlk2, ActRIA, Acvr, ActR-I, Alk-2, ALK2, Alk8, D330013D15Rik, Tsk7L, SKR1, Acvr1a
Type: Gene
Species: Mouse
Chromosome: 2
NCBI: 11477
HGNC: HGNC:171
Homologene: 7
Ift172
Name: intraflagellar transport 172
Synonyms: wim, 4930553F24Rik, avc1
Type: Gene
Species: Mouse
Chromosome: 5
NCBI: 67661
Homologene: 15202
Skor2
Name: SKI family transcriptional corepressor 2
Synonyms: Fussel18, Corl2, Gm7348
Type: Gene
Species: Mouse
Chromosome: 18
NCBI: 664805
VEGA: 18
Homologene: 64797
Fat3
Name: FAT atypical cadherin 3
Synonyms: D430038H04Rik, LOC382129, 9430076A06Rik, LOC234973
Type: Gene
Species: Mouse
Chromosome: 9
NCBI: 270120
VEGA: 9
Homologene: 82252
A1bg
Name: alpha-1-B glycoprotein
Synonyms: C44
Type: Gene
Species: Mouse
Chromosome: 15
NCBI: 117586
VEGA: 15
HGNC: HGNC:5
Homologene: 11167
Hmgb4
Name: high-mobility group box 4
Synonyms: 1700001F22Rik
Type: Gene
Species: Mouse
Chromosome: 4
NCBI: 69317
Homologene: 83321
Pmfbp1
Name: polyamine modulated factor 1 binding protein 1
Synonyms: 1700016D22Rik, F77
Type: Gene
Species: Mouse
Chromosome: 8
NCBI: 56523
Homologene: 23182
Iigp1
Name: interferon inducible GTPase 1
Synonyms: 2900074L10Rik, Irga6
Type: Gene
Species: Mouse
Chromosome: 18
NCBI: 60440
VEGA: 18
Gsdme
Name: gasdermin E
Synonyms: Dfna5h, Dfna5, 4932441K13Rik, Fin15, 2310037D07Rik
Type: Gene
Species: Mouse
Chromosome: 6
NCBI: 54722
HGNC: HGNC:2810
Homologene: 3242
Plcl1
Name: phospholipase C-like 1
Synonyms: C230017K02Rik, PRIP-1, PLC-L
Type: Gene
Species: Mouse
Chromosome: 1
NCBI: 227120
HGNC: HGNC:9063
Homologene: 38155
Gpat2
Name: glycerol-3-phosphate acyltransferase 2, mitochondrial
Synonyms: A530057A03Rik, Gpat2
Type: Gene
Species: Mouse
Chromosome: 2
NCBI: 215456
Homologene: 19037
B4galnt3
Name: beta-1,4-N-acetyl-galactosaminyl transferase 3
Type: Gene
Species: Mouse
Chromosome: 6
NCBI: 330406
Homologene: 18328
Or2v1
Name: olfactory receptor family 2 subfamily V member 1
Synonyms: Olfr56, MOR276-1, GA_x6K02T2QP88-6300500-6299553, IF7
Type: Gene
Species: Mouse
Chromosome: 11
NCBI: 18356
HGNC: HGNC:8280
Homologene: 22608
Lilra6
Name: leukocyte immunoglobulin-like receptor, subfamily A (with TM domain), member 6
Synonyms: 7M1, Pira3
Type: Gene
Species: Mouse
Chromosome: 7
NCBI: 18726
Homologene: 134028
Cyp2c55
Name: cytochrome P450, family 2, subfamily c, polypeptide 55
Synonyms: 2010318C06Rik
Type: Gene
Species: Mouse
Chromosome: 19
NCBI: 72082
HGNC: HGNC:2620
Homologene: 133567
Cdc14b
Name: CDC14 cell division cycle 14B
Synonyms: A530086E13Rik, 2810432N10Rik
Type: Gene
Species: Mouse
Chromosome: 13
NCBI: 218294
VEGA: 13
Homologene: 104197
Trim52
Name: tripartite motif-containing 52
Synonyms: 4921513B05Rik
Type: Gene
Species: Mouse
Chromosome: 14
NCBI: 212085
Homologene: 79723
Or51a5
Name: olfactory receptor family 51 subfamily A member 5
Synonyms: MOR8-7, Olfr586, GA_x6K02T2PBJ9-5836380-5835439, MOR8-2
Type: Gene
Species: Mouse
Chromosome: 7
NCBI: 259115
Homologene: 131143
Abca7
Name: ATP-binding cassette, sub-family A member 7
Synonyms: Abc51
Type: Gene
Species: Mouse
Chromosome: 10
NCBI: 27403
HGNC: HGNC:37
Homologene: 22783
Grin2d
Name: glutamate receptor, ionotropic, NMDA2D (epsilon 4)
Synonyms: GluN2D, NR2D, GluRepsilon4, NMDAR2D
Type: Gene
Species: Mouse
Chromosome: 7
NCBI: 14814
HGNC: HGNC:4588
Homologene: 648
Rps19bp1
Name: ribosomal protein S19 binding protein 1
Synonyms: S19BP, 2510038A11Rik
Type: Gene
Species: Mouse
Chromosome: 15
NCBI: 66538
VEGA: 15
Homologene: 18407
Dmrtb1
Name: DMRT-like family B with proline-rich C-terminal, 1
Synonyms: Dmrt6, Prp13
Type: Gene
Species: Mouse
Chromosome: 4
NCBI: 56296
Homologene: 13207
Tapbpl
Name: TAP binding protein-like
Synonyms: TAPBPL-R
Type: Gene
Species: Mouse
Chromosome: 6
NCBI: 213233
Homologene: 9958
Oas1b
Name: 2'-5' oligoadenylate synthetase 1B
Synonyms: Oias2, Wnv, Oias-2, Mmu-L1, Flavivirus resistance, Flv, L1
Type: Gene
Species: Mouse
Chromosome: 5
NCBI: 23961
HGNC: HGNC:8086
Genetic Alterations
ENU-induced transitions at the following base pair locations (GRCm38):
  • T to A, chromosome 1 at 55,697,958 bp
  • G to T, chromosome 1 at 173,341,709 bp
  • A to T, chromosome 1 at 183,331,485 bp
  • G to A, chromosome 2 at 58,479,757 bp
  • G to C, chromosome 2 at 127,431,918 bp
  • T to C, chromosome 4 at 107,679,345 bp
  • T to A, chromosome 4 at 128,260,595 bp
  • T to C, chromosome 4 at 130,019,276 bp
  • G to A, chromosome 5 at 31,275,883 bp
  • A to G, chromosome 5 at 75,645,853 bp
  • C to A, chromosome 5 at 120,814,546 bp
  • T to C, chromosome 6 at 50,229,449 bp
  • G to A, chromosome 6 at 120,217,479 bp
  • A to G, chromosome 6 at 125,228,344 bp
  • A to T, chromosome 7 at 3,915,020 bp
  • A to G, chromosome 7 at 45,834,755 bp
  • T to C, chromosome 7 at 103,122,238 bp
  • C to T, chromosome 8 at 109,525,428 bp
  • T to C, chromosome 9 at 15,915,327 bp
  • C to T, chromosome 10 at 80,011,788 bp
  • A to G, chromosome 11 at 49,134,812 bp
  • T to C, chromosome 13 at 64,225,630 bp
  • G to A, chromosome 14 at 106,107,219 bp
  • C to A, chromosome 15 at 60,920,810 bp
  • CCTTCTTCTTCTTCTTCTTCTT to CCTTCTTCTTCTTCTTCTT, chromosome 15 at 80,261,049 bp
  • T to A, chromosome 18 at 60,390,767 bp
  • C to G, chromosome 18 at 76,859,912 bp
  • A to C, chromosome 19 at 39,042,122 bp
Phenotype
G1 male heterozygous mice are viable and fertile. The G1 mice were not characterized prior to archiving.
Strain Development
C57BL/6J mice were injected with ENU, resulting in G0 mice that were bred back to C57BL/6J. Sperm was archived from G1 male mice that produced viable G2 mice.
Suggested Control Mice
Wild-type littermates or C57BL/6J mice
MMRRC Genetic QC Summary
The MMRRC Centers have developed a genetic QC pipeline using MiniMUGA array genotyping to provide additional information on strain backgrounds for MMRRC congenic and inbred strains. For more information on when data may be available, or to request genotyping for a strain of interest, please contact mmrrc@missouri.edu. Older strains may not have this information.
Donor
Bruce Beutler, M.D., University of Texas Southwestern Medical Center.
Primary Reference
MUTAGENETIX record for R6565 (G1). B. Beutler and colleagues, Center for the Genetics of Host Defense, UT Southwestern, Dallas, TX.

Colony and Husbandry Information

Colony Surveillance Program and Current Health Reports

Mice recovered from a cryo-archive will have health surveillance performed on recipient females. Health reports will be provided prior to shipment. If you require additional health status information, please email mmrrc@missouri.edu.
Coat Color
Black
MMRRC Breeding System
Random intra-strain mating
Breeding Scheme(s)
When maintaining a live colony, heterozygous mice may be bred together, bred with wild-type siblings, or bred with C57BL/6J inbred mice.
Overall Breeding Performance
Undetermined; during development, mating was done with heterozygous males; viability, fertility and other breeding statistics for heterozygous females and homozygotes are unverified, but presumably would be similar to the C57BL/6J background strain
Average litter size
Undetermined
Recommended wean age
3 weeks

Order Request Information

Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.

Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.

The donor or their institution limits the distribution to non-profit institutions only.

Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.

Click button to Request this one strain. (Use the MMRRC Catalog Search to request more than one strain.)
MMRRC Item # Description Distribution Fee / Unit (US $)
*Shipping & Handling not included*
Units Notes
044689-MU-RESUS Litter recovered from cryo-archive $5,475.00 / Non-Profit Litter Recovered litter4; additional fees for any special requests.
Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

1 The distribution fee covers the expense of rederiving mice from a live mouse; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

2 An aliquot contains a sufficient number of embryos (in one or more vials or straws and based on the transfer success rate of the MMRRC facility) to transfer into one to three recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.

3 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.

4 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.