Strain Name:
C57BL/6J-MtgxR6568Btlr/Mmmh
Stock Number:
044692-MU
Citation ID:
RRID:MMRRC_044692-MU
Other Names:
R6568 (G1)
Major Collection:

Strain Information

Col2a1
Name: collagen, type II, alpha 1
Synonyms: Del1, Col2a, Col2, M100856, Lpk, Rgsc856, Col2a-1, M100413, Rgsc413
Type: Gene
Species: Mouse
Chromosome: 15
NCBI: 12824
HGNC: HGNC:2200
Homologene: 55607
Fryl
Name: FRY like transcription coactivator
Synonyms: 2310004H21Rik, 9030227G01Rik, 2510002A14Rik
Type: Gene
Species: Mouse
Chromosome: 5
NCBI: 72313
Homologene: 103956
Polr3b
Name: polymerase (RNA) III (DNA directed) polypeptide B
Synonyms: A330032P03Rik, 2700078H01Rik, RPC2
Type: Gene
Species: Mouse
Chromosome: 10
NCBI: 70428
VEGA: 10
Homologene: 6981
Taf2
Name: TATA-box binding protein associated factor 2
Synonyms: 4732460C16Rik, TAF2B, TAFII150, 150kDa, CIF150
Type: Gene
Species: Mouse
Chromosome: 15
NCBI: 319944
VEGA: 15
Homologene: 31137
Nek1
Name: NIMA (never in mitosis gene a)-related expressed kinase 1
Synonyms: kat, kidney, anemia and testis, D8Ertd790e
Type: Gene
Species: Mouse
Chromosome: 8
NCBI: 18004
HGNC: HGNC:7744
Homologene: 14376
Ash1l
Name: ASH1 like histone lysine methyltransferase
Synonyms: 8030453L17Rik, KMT2H, chromatin remodeling factor, E430018P19Rik
Type: Gene
Species: Mouse
Chromosome: 3
NCBI: 192195
Homologene: 10225
Trpm2
Name: transient receptor potential cation channel, subfamily M, member 2
Synonyms: TRPC7, Trrp7, 9830168K16Rik, LTRPC2
Type: Gene
Species: Mouse
Chromosome: 10
NCBI: 28240
Homologene: 20709
Zfp942
Name: zinc finger protein 942
Synonyms: 3110048L19Rik
Type: Gene
Species: Mouse
Chromosome: 17
NCBI: 73233
Homologene: 133237
Ccr5
Name: C-C motif chemokine receptor 5
Synonyms: CD195, Cmkbr5
Type: Gene
Species: Mouse
Chromosome: 9
NCBI: 12774
HGNC: HGNC:1606
Homologene: 37325
Ros1
Name: Ros1 proto-oncogene
Synonyms: Ros-1, c-ros
Type: Gene
Species: Mouse
Chromosome: 10
NCBI: 19886
Homologene: 2207
Myo1c
Name: myosin IC
Synonyms: mm1beta, C80397, myr2, myosin-Ibeta
Type: Gene
Species: Mouse
Chromosome: 11
NCBI: 17913
HGNC: HGNC:7597
Homologene: 32046
Slc34a2
Name: solute carrier family 34 (sodium phosphate), member 2
Synonyms: D5Ertd227e, NaPi-2b, type IIb Na/Picotransporter, Npt2b
Type: Gene
Species: Mouse
Chromosome: 5
NCBI: 20531
Homologene: 2297
Mplkip
Name: M-phase specific PLK1 intereacting protein
Synonyms: 2810021B07Rik, C330007M08Rik
Type: Gene
Species: Mouse
Chromosome: 13
NCBI: 66308
VEGA: 13
Homologene: 32633
Kdr
Name: kinase insert domain protein receptor
Synonyms: VEGF receptor-2, VEGFR-2, VEGFR2, orv, vascular endothelial growth factor receptor- 2, Flk-1, Flk1
Type: Gene
Species: Mouse
Chromosome: 5
NCBI: 16542
HGNC: HGNC:6307
Homologene: 55639
Rgsl1
Name: regulator of G-protein signaling like 1
Synonyms: Rgsl2, 4930415K13Rik
Type: Gene
Species: Mouse
Chromosome: 1
NCBI: 240816
Homologene: 129962
Miip
Name: migration and invasion inhibitory protein
Synonyms: D4Wsu114e
Type: Gene
Species: Mouse
Chromosome: 4
NCBI: 28010
Homologene: 15879
Doc2b
Name: double C2, beta
Type: Gene
Species: Mouse
Chromosome: 11
NCBI: 13447
HGNC: HGNC:2986
Homologene: 20796
Ceacam5
Name: CEA cell adhesion molecule 5
Synonyms: 1600029H12Rik, Psg30
Type: Gene
Species: Mouse
Chromosome: 7
NCBI: 73250
HGNC: HGNC:1819
Homologene: 115938
Ms4a13
Name: membrane-spanning 4-domains, subfamily A, member 13
Synonyms: 1700060E18Rik
Type: Gene
Species: Mouse
Chromosome: 19
NCBI: 73466
Homologene: 77851
Or5ac17
Name: olfactory receptor family 5 subfamily AC member 17
Synonyms: Olfr199, GA_x54KRFPKG5P-55430495-55429569, MOR182-14
Type: Gene
Species: Mouse
Chromosome: 16
NCBI: 404310
Homologene: 37011
Tlr12
Name: toll-like receptor 12
Synonyms: Tlr11, LOC384059
Type: Gene
Species: Mouse
Chromosome: 4
NCBI: 384059
Homologene: 135964
Hdac1-ps
Name: histone deacetylase 1, pseudogene
Synonyms: Gm10093, EG15181
Type: Gene
Species: Mouse
Chromosome: 17
NCBI: 15181
VEGA: 17
Bhmt1b
Name: betaine--homocysteine S-methyltransferase 1B
Synonyms: Gm5096
Type: Gene
Species: Mouse
Chromosome: 18
NCBI: 329008
VEGA: 18
HGNC: HGNC:1047
2310009B15Rik
Name: RIKEN cDNA 2310009B15 gene
Type: Gene
Species: Mouse
Chromosome: 1
NCBI: 69549
Homologene: 19923
Ighv1-34
Name: immunoglobulin heavy variable 1-34
Synonyms: Gm16735
Type: Gene
Species: Mouse
Chromosome: 12
NCBI: 628614
Genetic Alterations
ENU-induced transitions at the following base pair locations (GRCm38):
  • T to C, chromosome 1 at 138,852,134 bp
  • A to C, chromosome 1 at 153,821,546 bp
  • G to A, chromosome 3 at 89,052,037 bp
  • T to C, chromosome 4 at 128,617,992 bp
  • T to A, chromosome 4 at 147,865,915 bp
  • T to C, chromosome 5 at 53,069,134 bp
  • T to C, chromosome 5 at 73,059,516 bp
  • A to G, chromosome 5 at 75,961,774 bp
  • C to T, chromosome 7 at 17,745,491 bp
  • T to C, chromosome 8 at 61,106,821 bp
  • G to A, chromosome 9 at 124,125,199 bp
  • A to T, chromosome 10 at 52,162,812 bp
  • C to T, chromosome 10 at 77,937,826 bp
  • T to A, chromosome 10 at 84,634,903 bp
  • C to T, chromosome 11 at 75,671,635 bp
  • A to C, chromosome 11 at 75,776,994 bp
  • A to T, chromosome 12 at 114,851,611 bp
  • T to C, chromosome 13 at 17,695,677 bp
  • G to A, chromosome 15 at 55,064,630 bp
  • T to C, chromosome 15 at 97,977,276 bp
  • A to T, chromosome 16 at 59,216,278 bp
  • T to G, chromosome 17 at 21,929,062 bp
  • A to G, chromosome 17 at 78,492,588 bp
  • A to T, chromosome 18 at 87,757,442 bp
  • A to G, chromosome 19 at 11,191,559 bp
Phenotype
G1 male heterozygous mice are viable and fertile. The G1 mice were not characterized prior to archiving.
Strain Development
C57BL/6J mice were injected with ENU, resulting in G0 mice that were bred back to C57BL/6J. Sperm was archived from G1 male mice that produced viable G2 mice.
Suggested Control Mice
Wild-type littermates or C57BL/6J mice
MMRRC Genetic QC Summary
The MMRRC Centers have developed a genetic QC pipeline using MiniMUGA array genotyping to provide additional information on strain backgrounds for MMRRC congenic and inbred strains. For more information on when data may be available, or to request genotyping for a strain of interest, please contact mmrrc@missouri.edu. Older strains may not have this information.
Donor
Bruce Beutler, M.D., University of Texas Southwestern Medical Center.
Primary Reference
MUTAGENETIX record for R6568 (G1). B. Beutler and colleagues, Center for the Genetics of Host Defense, UT Southwestern, Dallas, TX.

Colony and Husbandry Information

Colony Surveillance Program and Current Health Reports

Mice recovered from a cryo-archive will have health surveillance performed on recipient females. Health reports will be provided prior to shipment. If you require additional health status information, please email mmrrc@missouri.edu.
Coat Color
Black
MMRRC Breeding System
Random intra-strain mating
Breeding Scheme(s)
When maintaining a live colony, heterozygous mice may be bred together, bred with wild-type siblings, or bred with C57BL/6J inbred mice.
Overall Breeding Performance
Undetermined; during development, mating was done with heterozygous males; viability, fertility and other breeding statistics for heterozygous females and homozygotes are unverified, but presumably would be similar to the C57BL/6J background strain
Average litter size
Undetermined
Recommended wean age
3 weeks

Order Request Information

Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.

Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.

The donor or their institution limits the distribution to non-profit institutions only.

Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.

Click button to Request this one strain. (Use the MMRRC Catalog Search to request more than one strain.)
MMRRC Item # Description Distribution Fee / Unit (US $)
*Shipping & Handling not included*
Units Notes
044692-MU-RESUS Litter recovered from cryo-archive $5,475.00 / Non-Profit Litter Recovered litter4; additional fees for any special requests.
Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

1 The distribution fee covers the expense of rederiving mice from a live mouse; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

2 An aliquot contains a sufficient number of embryos (in one or more vials or straws and based on the transfer success rate of the MMRRC facility) to transfer into one to three recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.

3 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.

4 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.