Strain Name:
C57BL/6J-MtgxR6586Btlr/Mmmh
Stock Number:
044710-MU
Citation ID:
RRID:MMRRC_044710-MU
Other Names:
R6586 (G1)
Major Collection:

Strain Information

Fiz1
Name: Flt3 interacting zinc finger protein 1
Type: Gene
Species: Mouse
Chromosome: 7
NCBI: 23877
Homologene: 22663
Pipox
Name: pipecolic acid oxidase
Synonyms: Pso
Type: Gene
Species: Mouse
Chromosome: 11
NCBI: 19193
Homologene: 40640
Urb2
Name: URB2 ribosome biogenesis 2 homolog (S. cerevisiae)
Type: Gene
Species: Mouse
Chromosome: 8
NCBI: 382038
Homologene: 8859
Clasp2
Name: CLIP associating protein 2
Synonyms: CLASP2beta, 1500004F14Rik, CLASP2gamma, 8030404L10Rik, CLASP2alpha, CLASP2
Type: Gene
Species: Mouse
Chromosome: 9
NCBI: 76499
VEGA: 9
Homologene: 24944
Tnpo2
Name: transportin 2 (importin 3, karyopherin beta 2b)
Synonyms: 1110034O24Rik, TRN2, Kpnb2b
Type: Gene
Species: Mouse
Chromosome: 8
NCBI: 212999
Homologene: 8381
Ythdf2
Name: YTH N6-methyladenosine RNA binding protein 2
Synonyms: 9430020E02Rik, NY-REN-2, HGRG8
Type: Gene
Species: Mouse
Chromosome: 4
NCBI: 213541
Homologene: 32302
Ythdc2
Name: YTH domain containing 2
Synonyms: 3010002F02Rik
Type: Gene
Species: Mouse
Chromosome: 18
NCBI: 240255
Homologene: 11265
Styxl2
Name: serine/threonine/tyrosine interacting like 2
Synonyms: Dusp27, C130085G02Rik
Type: Gene
Species: Mouse
Chromosome: 1
NCBI: 240892
Homologene: 18949
Tmco3
Name: transmembrane and coiled-coil domains 3
Synonyms: B230339H12Rik
Type: Gene
Species: Mouse
Chromosome: 8
NCBI: 234076
Homologene: 32376
Ttn
Name: titin
Synonyms: 2310036G12Rik, 2310057K23Rik, D330041I19Rik, mdm, connectin, D830007G01Rik, 1100001C23Rik, shru, 2310074I15Rik, L56
Type: Gene
Species: Mouse
Chromosome: 2
NCBI: 22138
Homologene: 130650
Egfem1
Name: EGF-like and EMI domain containing 1
Synonyms: 6130401L20Rik
Type: Gene
Species: Mouse
Chromosome: 3
NCBI: 75740
Homologene: 135950
Ogfrl1
Name: opioid growth factor receptor-like 1
Synonyms: 2210417C17Rik
Type: Gene
Species: Mouse
Chromosome: 1
NCBI: 70155
Homologene: 11595
Cngb3
Name: cyclic nucleotide gated channel beta 3
Synonyms: CCNC2, CNG6, Cngbeta2
Type: Gene
Species: Mouse
Chromosome: 4
NCBI: 30952
HGNC: HGNC:2153
Homologene: 40908
Nlrp1a
Name: NLR family, pyrin domain containing 1A
Synonyms: Nalp1
Type: Gene
Species: Mouse
Chromosome: 11
NCBI: 195046
Homologene: 133820
Flg
Name: filaggrin
Synonyms: fillagrin, ft, profilaggrin
Type: Gene
Species: Mouse
Chromosome: 3
NCBI: 14246
VEGA: 3
Psd3
Name: pleckstrin and Sec7 domain containing 3
Synonyms: 4931420C21Rik, EFA6D
Type: Gene
Species: Mouse
Chromosome: 8
NCBI: 234353
Homologene: 87257
Trim60
Name: tripartite motif-containing 60
Synonyms: Rnf33, 2czf45
Type: Gene
Species: Mouse
Chromosome: 8
NCBI: 234329
Homologene: 51876
Tas2r135
Name: taste receptor, type 2, member 135
Synonyms: Tas2r35, mt2r38
Type: Gene
Species: Mouse
Chromosome: 6
NCBI: 387512
Homologene: 52230
Tns4
Name: tensin 4
Synonyms: 9930017A07Rik
Type: Gene
Species: Mouse
Chromosome: 11
NCBI: 217169
Homologene: 13147
Cyp2c65
Name: cytochrome P450, family 2, subfamily c, polypeptide 65
Synonyms: 2210009K14Rik
Type: Gene
Species: Mouse
Chromosome: 19
NCBI: 72303
Homologene: 133566
Vmn1r60
Name: vomeronasal 1 receptor 60
Synonyms: Gm7184
Type: Gene
Species: Mouse
Chromosome: 7
NCBI: 636697
Homologene: 41799
Fah
Name: fumarylacetoacetate hydrolase
Synonyms: swst
Type: Gene
Species: Mouse
Chromosome: 7
NCBI: 14085
HGNC: HGNC:3579
Homologene: 110
Psg28
Name: pregnancy-specific beta-1-glycoprotein 28
Type: Gene
Species: Mouse
Chromosome: 7
NCBI: 114871
Homologene: 110989
Rbbp8nl
Name: RBBP8 N-terminal like
Synonyms: BC066135
Type: Gene
Species: Mouse
Chromosome: 2
NCBI: 271887
Homologene: 76811
Or51v14
Name: olfactory receptor family 51 subfamily V member 14
Synonyms: Olfr620, GA_x6K02T2PBJ9-6335095-6334154, MOR4-1
Type: Gene
Species: Mouse
Chromosome: 7
NCBI: 258808
Homologene: 103777
1700013G24Rik
Name: RIKEN cDNA 1700013G24 gene
Type: Gene
Species: Mouse
Chromosome: 4
NCBI: 69380
Homologene: 124477
Vps25
Name: vacuolar protein sorting 25
Synonyms: 1110020N13Rik, D11Wsu68e
Type: Gene
Species: Mouse
Chromosome: 11
NCBI: 28084
Homologene: 6303
Acaa1a
Name: acetyl-Coenzyme A acyltransferase 1A
Synonyms: PTL, Acaa1, D9Ertd25e, thiolase A, peroxisomal 3-ketoacyl-CoA thiolase
Type: Gene
Species: Mouse
Chromosome: 9
NCBI: 113868
HGNC: HGNC:82
Homologene: 37497
Mterf2
Name: mitochondrial transcription termination factor 2
Synonyms: Mterfd3, 1700007D05Rik
Type: Gene
Species: Mouse
Chromosome: 10
NCBI: 74238
VEGA: 10
Homologene: 11869
Or1o1
Name: olfactory receptor family 1 subfamily O member 1
Synonyms: MOR156-3, GA_x6K02T2PSCP-1867165-1868094, Olfr107
Type: Gene
Species: Mouse
Chromosome: 17
NCBI: 258504
Homologene: 115498
9930022D16Rik
Name: RIKEN cDNA 9930022D16 gene
Type: Gene
Species: Mouse
Chromosome: 11
Nrip2
Name: nuclear receptor interacting protein 2
Synonyms: NIX1
Type: Gene
Species: Mouse
Chromosome: 6
NCBI: 60345
Homologene: 11023
Cnga3
Name: cyclic nucleotide gated channel alpha 3
Synonyms: CNG3
Type: Gene
Species: Mouse
Chromosome: 1
NCBI: 12790
HGNC: HGNC:2150
Homologene: 994
Rarres1
Name: retinoic acid receptor responder (tazarotene induced) 1
Synonyms: 5430417P09Rik
Type: Gene
Species: Mouse
Chromosome: 3
NCBI: 109222
HGNC: HGNC:9867
Homologene: 2166
Dnase1l1
Name: deoxyribonuclease 1-like 1
Synonyms: Dnl1ll, 2310005K03Rik, G4.8, Dnase1ll
Type: Gene
Species: Mouse
Chromosome: X
NCBI: 69537
HGNC: HGNC:2957
Homologene: 4896
Genetic Alterations
ENU-induced transitions at the following base pair locations (GRCm38):
  • T to G, chromosome 1 at 23,369,863 bp
  • T to C, chromosome 1 at 37,261,278 bp
  • T to C, chromosome 1 at 166,100,885 bp
  • T to C, chromosome 2 at 76,730,410 bp
  • G to A, chromosome 2 at 180,280,959 bp
  • T to A, chromosome 3 at 29,662,411 bp
  • A to T, chromosome 3 at 67,491,033 bp
  • A to T, chromosome 3 at 93,292,983 bp
  • T to G, chromosome 4 at 19,280,946 bp
  • A to T, chromosome 4 at 132,205,600 bp
  • T to C, chromosome 4 at 137,455,328 bp
  • A to G, chromosome 6 at 42,406,018 bp
  • T to A, chromosome 6 at 128,404,948 bp
  • C to T, chromosome 7 at 5,008,401 bp
  • T to A, chromosome 7 at 5,544,447 bp
  • T to C, chromosome 7 at 18,430,544 bp
  • T to A, chromosome 7 at 84,593,260 bp
  • T to A, chromosome 7 at 103,611,976 bp
  • G to T, chromosome 8 at 13,320,894 bp
  • G to T, chromosome 8 at 65,000,596 bp
  • T to C, chromosome 8 at 67,963,545 bp
  • T to A, chromosome 8 at 85,045,202 bp
  • G to T, chromosome 8 at 124,031,125 bp
  • T to C, chromosome 9 at 21,505,646 bp
  • T to C, chromosome 9 at 113,813,264 bp
  • A to G, chromosome 9 at 119,349,538 bp
  • A to C, chromosome 10 at 79,809,531 bp
  • A to G, chromosome 10 at 85,120,106 bp
  • A to G, chromosome 11 at 71,106,073 bp
  • T to C, chromosome 11 at 77,881,179 bp
  • T to C, chromosome 11 at 99,080,267 bp
  • T to G, chromosome 11 at 101,259,009 bp
  • A to G, chromosome 11 at 109,417,960 bp
  • A to G, chromosome 14 at 7,929,138 bp
  • C to T, chromosome 15 at 76,175,087 bp
  • G to A, chromosome 17 at 37,405,905 bp
  • A to G, chromosome 18 at 44,845,788 bp
  • T to C, chromosome 19 at 39,082,218 bp
  • C to T, chromosome X at 74,277,038 bp
Phenotype
G1 male heterozygous mice are viable and fertile. The G1 mice were not characterized prior to archiving.
Strain Development
C57BL/6J mice were injected with ENU, resulting in G0 mice that were bred back to C57BL/6J. Sperm was archived from G1 male mice that produced viable G2 mice.
Suggested Control Mice
Wild-type littermates or C57BL/6J mice
MMRRC Genetic QC Summary
The MMRRC Centers have developed a genetic QC pipeline using MiniMUGA array genotyping to provide additional information on strain backgrounds for MMRRC congenic and inbred strains. For more information on when data may be available, or to request genotyping for a strain of interest, please contact mmrrc@missouri.edu. Older strains may not have this information.
Donor
Bruce Beutler, M.D., University of Texas Southwestern Medical Center.
Primary Reference
MUTAGENETIX record for R6586 (G1). B. Beutler and colleagues, Center for the Genetics of Host Defense, UT Southwestern, Dallas, TX.

Colony and Husbandry Information

Colony Surveillance Program and Current Health Reports

Mice recovered from a cryo-archive will have health surveillance performed on recipient females. Health reports will be provided prior to shipment. If you require additional health status information, please email mmrrc@missouri.edu.
Coat Color
Black
MMRRC Breeding System
Random intra-strain mating
Breeding Scheme(s)
When maintaining a live colony, heterozygous mice may be bred together, bred with wild-type siblings, or bred with C57BL/6J inbred mice.
Overall Breeding Performance
Undetermined; during development, mating was done with heterozygous males; viability, fertility and other breeding statistics for heterozygous females and homozygotes are unverified, but presumably would be similar to the C57BL/6J background strain
Average litter size
Undetermined
Recommended wean age
3 weeks

Order Request Information

Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.

Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.

The donor or their institution limits the distribution to non-profit institutions only.

Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.

Click button to Request this one strain. (Use the MMRRC Catalog Search to request more than one strain.)
MMRRC Item # Description Distribution Fee / Unit (US $)
*Shipping & Handling not included*
Units Notes
044710-MU-RESUS Litter recovered from cryo-archive $5,475.00 / Non-Profit Litter Recovered litter4; additional fees for any special requests.
Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

1 The distribution fee covers the expense of rederiving mice from a live mouse; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

2 An aliquot contains a sufficient number of embryos (in one or more vials or straws and based on the transfer success rate of the MMRRC facility) to transfer into one to three recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.

3 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.

4 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.