Strain Name:
C57BL/6J-MtgxR6596Btlr/Mmmh
Stock Number:
044720-MU
Citation ID:
RRID:MMRRC_044720-MU
Other Names:
R6596 (G1)
Major Collection:

Strain Information

Fn1
Name: fibronectin 1
Synonyms: Fn, Fn-1
Type: Gene
Species: Mouse
Chromosome: 1
NCBI: 14268
HGNC: HGNC:3778
Homologene: 1533
Syngr1
Name: synaptogyrin 1
Synonyms: p29, Syngr1b
Type: Gene
Species: Mouse
Chromosome: 15
NCBI: 20972
Homologene: 3456
Nsf
Name: N-ethylmaleimide sensitive fusion protein
Synonyms: N-ethylmaleimide sensitive factor, SKD2
Type: Gene
Species: Mouse
Chromosome: 11
NCBI: 18195
HGNC: HGNC:8016
Homologene: 4502
Xrcc6
Name: X-ray repair complementing defective repair in Chinese hamster cells 6
Synonyms: Ku p70, G22p1, Ku70
Type: Gene
Species: Mouse
Chromosome: 15
NCBI: 14375
HGNC: HGNC:4055
Homologene: 37483
Tubgcp5
Name: tubulin, gamma complex component 5
Synonyms: GCP5, B130010C12Rik
Type: Gene
Species: Mouse
Chromosome: 7
NCBI: 233276
Homologene: 14172
Smc4
Name: structural maintenance of chromosomes 4
Synonyms: Smc4l1, 2500002A22Rik
Type: Gene
Species: Mouse
Chromosome: 3
NCBI: 70099
Homologene: 4015
Garem1
Name: GRB2 associated regulator of MAPK1 subtype 1
Synonyms: LOC381126, Fam59a, Garem
Type: Gene
Species: Mouse
Chromosome: 18
NCBI: 381126
VEGA: 18
Homologene: 11237
Gfm2
Name: G elongation factor, mitochondrial 2
Synonyms: EFG2, A930009M04Rik, MST027, 6530419G12Rik
Type: Gene
Species: Mouse
Chromosome: 13
NCBI: 320806
Homologene: 6238
Robo2
Name: roundabout guidance receptor 2
Synonyms: D230004I22Rik, 2600013A04Rik, 9430089E08Rik
Type: Gene
Species: Mouse
Chromosome: 16
NCBI: 268902
Homologene: 43188
Plk2
Name: polo like kinase 2
Synonyms: Snk
Type: Gene
Species: Mouse
Chromosome: 13
NCBI: 20620
VEGA: 13
Homologene: 21317
Muc16
Name: mucin 16
Synonyms: 1110008I14Rik, LOC385009, Gm21044
Type: Gene
Species: Mouse
Chromosome: 9
NCBI: 73732
Homologene: 141193
Sorl1
Name: sortilin-related receptor, LDLR class A repeats-containing
Synonyms: Sorla, LR11, mSorLA, 2900010L19Rik
Type: Gene
Species: Mouse
Chromosome: 9
NCBI: 20660
Homologene: 2336
Kmt5a
Name: lysine methyltransferase 5A
Synonyms: PR-SET7, Setd8, 2410195B05Rik
Type: Gene
Species: Mouse
Chromosome: 5
NCBI: 67956
Homologene: 41372
Vit
Name: vitrin
Synonyms: 2810429K11Rik, akhirin, 1700110E08Rik, AKH, 1700052E02Rik
Type: Gene
Species: Mouse
Chromosome: 17
NCBI: 74199
VEGA: 17
Homologene: 24942
Ucp3
Name: uncoupling protein 3 (mitochondrial, proton carrier)
Synonyms: Slc25a9, UCP-3
Type: Gene
Species: Mouse
Chromosome: 7
NCBI: 22229
Homologene: 2517
Tns2
Name: tensin 2
Synonyms: nep, nph, Tenc1
Type: Gene
Species: Mouse
Chromosome: 15
NCBI: 209039
VEGA: 15
Homologene: 37077
Slc35f4
Name: solute carrier family 35, member F4
Synonyms: 4930550L21Rik
Type: Gene
Species: Mouse
Chromosome: 14
NCBI: 75288
Homologene: 45939
Col7a1
Name: collagen, type VII, alpha 1
Type: Gene
Species: Mouse
Chromosome: 9
NCBI: 12836
HGNC: HGNC:2214
Homologene: 73
Dennd4a
Name: DENN domain containing 4A
Synonyms: F730015K02Rik
Type: Gene
Species: Mouse
Chromosome: 9
NCBI: 102442
VEGA: 9
Homologene: 55933
Tbx15
Name: T-box 15
Synonyms: Tbx14, de, Tbx8
Type: Gene
Species: Mouse
Chromosome: 3
NCBI: 21384
Homologene: 7967
Duox2
Name: dual oxidase 2
Synonyms: A430065P05Rik
Type: Gene
Species: Mouse
Chromosome: 2
NCBI: 214593
Homologene: 9689
Pomgnt2
Name: protein O-linked mannose beta 1,4-N-acetylglucosaminyltransferase 2
Synonyms: Gtdc2, C85492
Type: Gene
Species: Mouse
Chromosome: 9
NCBI: 215494
VEGA: 9
Homologene: 32795
Ephb1
Name: Eph receptor B1
Synonyms: Net, Elkh, Elk, C130099E04Rik, Hek6, Cek6
Type: Gene
Species: Mouse
Chromosome: 9
NCBI: 270190
HGNC: HGNC:3392
Homologene: 20936
Fam149a
Name: family with sequence similarity 149, member A
Type: Gene
Species: Mouse
Chromosome: 8
NCBI: 212326
Homologene: 27540
Tbc1d16
Name: TBC1 domain family, member 16
Type: Gene
Species: Mouse
Chromosome: 11
NCBI: 207592
Homologene: 10380
Dsg1c
Name: desmoglein 1 gamma
Synonyms: Dsg6
Type: Gene
Species: Mouse
Chromosome: 18
NCBI: 211924
HGNC: HGNC:3048
Tpte
Name: transmembrane phosphatase with tensin homology
Synonyms: Pten2, Vsp
Type: Gene
Species: Mouse
Chromosome: 8
NCBI: 234129
Homologene: 50411
Pcdhb7
Name: protocadherin beta 7
Synonyms: Pcdhb4B, PcdhbG
Type: Gene
Species: Mouse
Chromosome: 18
NCBI: 93878
HGNC: HGNC:8689
Homologene: 87123
Rasgrf1
Name: RAS protein-specific guanine nucleotide-releasing factor 1
Synonyms: Grfbeta, CDC25, Grf1, CDC25Mm
Type: Gene
Species: Mouse
Chromosome: 9
NCBI: 19417
HGNC: HGNC:9875
Homologene: 74972
Mindy4
Name: MINDY lysine 48 deubiquitinase 4
Synonyms: Fam188b, LOC384387, C330043M08Rik
Type: Gene
Species: Mouse
Chromosome: 6
NCBI: 330323
Homologene: 49991
Cldn15
Name: claudin 15
Synonyms: 2210009B08Rik
Type: Gene
Species: Mouse
Chromosome: 5
NCBI: 60363
HGNC: HGNC:2036
Homologene: 8646
9630041A04Rik
Name: RIKEN cDNA 9630041A04 gene
Type: Gene
Species: Mouse
Chromosome: 9
Hyou1
Name: hypoxia up-regulated 1
Synonyms: Orp150, Cab140, CBP-140, Grp170, 140 kDa
Type: Gene
Species: Mouse
Chromosome: 9
NCBI: 12282
Homologene: 4658
Bag4
Name: BCL2-associated athanogene 4
Synonyms: 2410112I15Rik
Type: Gene
Species: Mouse
Chromosome: 8
NCBI: 67384
HGNC: HGNC:940
Homologene: 31270
Obox1
Name: oocyte specific homeobox 1
Synonyms: 7420700M11Rik
Type: Gene
Species: Mouse
Chromosome: 7
NCBI: 71468
Homologene: 44937
Or4b1
Name: olfactory receptor family 4 subfamily B member 1
Synonyms: MOR227-1, GA_x6K02T2Q125-51584440-51583526, Olfr1270
Type: Gene
Species: Mouse
Chromosome: 2
NCBI: 258987
HGNC: HGNC:8290
Homologene: 133648
Or5d38
Name: olfactory receptor family 5 subfamily D member 38
Synonyms: GA_x6K02T2Q125-49616865-49615915, Olfr1166, MOR174-6
Type: Gene
Species: Mouse
Chromosome: 2
NCBI: 258644
Homologene: 138307
Crnn
Name: cornulin
Synonyms: LOC381457
Type: Gene
Species: Mouse
Chromosome: 3
NCBI: 381457
HGNC: HGNC:1230
Homologene: 9416
Dcstamp
Name: dendrocyte expressed seven transmembrane protein
Synonyms: Tm7sf4, DC-STAMP, 4833414I07Rik
Type: Gene
Species: Mouse
Chromosome: 15
NCBI: 75766
VEGA: 15
Homologene: 12769
Eif1ad15
Name: eukaryotic translation initiation factor 1A domain containing 15
Synonyms: Gm5039
Type: Gene
Species: Mouse
Chromosome: 12
NCBI: 266459
VEGA: 12
Genetic Alterations
ENU-induced transitions at the following base pair locations (GRCm38):
  • A to G, chromosome 1 at 71,609,482 bp
  • C to T, chromosome 2 at 88,124,199 bp
  • T to A, chromosome 2 at 90,149,278 bp
  • C to T, chromosome 2 at 122,285,338 bp
  • A to T, chromosome 3 at 69,025,893 bp
  • G to A, chromosome 3 at 93,146,875 bp
  • A to G, chromosome 3 at 99,352,192 bp
  • G to A, chromosome 5 at 124,450,696 bp
  • T to A, chromosome 5 at 136,974,679 bp
  • T to C, chromosome 6 at 55,224,016 bp
  • C to T, chromosome 7 at 15,555,376 bp
  • T to A, chromosome 7 at 55,806,634 bp
  • A to T, chromosome 7 at 100,481,933 bp
  • T to C, chromosome 8 at 22,333,269 bp
  • C to T, chromosome 8 at 25,769,500 bp
  • G to T, chromosome 8 at 45,381,630 bp
  • T to C, chromosome 9 at 18,566,715 bp
  • T to G, chromosome 9 at 42,001,603 bp
  • A to G, chromosome 9 at 44,387,755 bp
  • A to G, chromosome 9 at 64,852,420 bp
  • A to T, chromosome 9 at 90,012,794 bp
  • T to A, chromosome 9 at 101,942,785 bp
  • A to C, chromosome 9 at 102,194,802 bp
  • C to A, chromosome 9 at 108,954,341 bp
  • T to C, chromosome 9 at 121,982,254 bp
  • A to T, chromosome 11 at 103,910,457 bp
  • A to C, chromosome 11 at 119,157,775 bp
  • C to A, chromosome 12 at 88,321,287 bp
  • C to T, chromosome 13 at 97,165,149 bp
  • C to T, chromosome 13 at 110,397,762 bp
  • G to A, chromosome 14 at 49,525,600 bp
  • A to C, chromosome 15 at 39,754,209 bp
  • C to T, chromosome 15 at 80,111,692 bp
  • T to C, chromosome 15 at 82,022,954 bp
  • G to A, chromosome 15 at 102,110,559 bp
  • T to A, chromosome 16 at 73,971,108 bp
  • T to C, chromosome 17 at 78,622,845 bp
  • T to A, chromosome 18 at 20,270,524 bp
  • T to A, chromosome 18 at 21,148,739 bp
  • A to T, chromosome 18 at 37,343,361 bp
Phenotype
G1 male heterozygous mice are viable and fertile. The G1 mice were not characterized prior to archiving.
Strain Development
C57BL/6J mice were injected with ENU, resulting in G0 mice that were bred back to C57BL/6J. Sperm was archived from G1 male mice that produced viable G2 mice.
Suggested Control Mice
Wild-type littermates or C57BL/6J mice
MMRRC Genetic QC Summary
The MMRRC Centers have developed a genetic QC pipeline using MiniMUGA array genotyping to provide additional information on strain backgrounds for MMRRC congenic and inbred strains. For more information on when data may be available, or to request genotyping for a strain of interest, please contact mmrrc@missouri.edu. Older strains may not have this information.
Donor
Bruce Beutler, M.D., University of Texas Southwestern Medical Center.
Primary Reference
MUTAGENETIX record for R6596 (G1). B. Beutler and colleagues, Center for the Genetics of Host Defense, UT Southwestern, Dallas, TX.

Colony and Husbandry Information

Colony Surveillance Program and Current Health Reports

Mice recovered from a cryo-archive will have health surveillance performed on recipient females. Health reports will be provided prior to shipment. If you require additional health status information, please email mmrrc@missouri.edu.
Coat Color
Black
MMRRC Breeding System
Random intra-strain mating
Breeding Scheme(s)
When maintaining a live colony, heterozygous mice may be bred together, bred with wild-type siblings, or bred with C57BL/6J inbred mice.
Overall Breeding Performance
Undetermined; during development, mating was done with heterozygous males; viability, fertility and other breeding statistics for heterozygous females and homozygotes are unverified, but presumably would be similar to the C57BL/6J background strain
Average litter size
Undetermined
Recommended wean age
3 weeks

Order Request Information

Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.

Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.

The donor or their institution limits the distribution to non-profit institutions only.

Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.

Click button to Request this one strain. (Use the MMRRC Catalog Search to request more than one strain.)
MMRRC Item # Description Distribution Fee / Unit (US $)
*Shipping & Handling not included*
Units Notes
044720-MU-RESUS Litter recovered from cryo-archive $5,475.00 / Non-Profit Litter Recovered litter4; additional fees for any special requests.
Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

1 The distribution fee covers the expense of rederiving mice from a live mouse; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

2 An aliquot contains a sufficient number of embryos (in one or more vials or straws and based on the transfer success rate of the MMRRC facility) to transfer into one to three recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.

3 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.

4 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.