Loading Mouse GIF
Loading...

Strain Name:
C57BL/6J-MtgxR6615Btlr/Mmmh
Stock Number:
044738-MU
Citation ID:
RRID:MMRRC_044738-MU
Other Names:
R6615 (G1)
Major Collection:

Strain Information

Ptch1
Name: patched 1
Synonyms: Ptc, Patched 1, Ptc1, A230106A15Rik, wig
Type: Gene
Species: Mouse
Chromosome: 13
NCBI: 19206
HGNC: HGNC:9585
Homologene: 223
Bcar3
Name: breast cancer anti-estrogen resistance 3
Synonyms: AND-34
Type: Gene
Species: Mouse
Chromosome: 3
NCBI: 29815
HGNC: HGNC:973
Homologene: 31181
Pcf11
Name: PCF11 cleavage and polyadenylation factor subunit
Synonyms: 5730417B17Rik, 2500001H09Rik
Type: Gene
Species: Mouse
Chromosome: 7
NCBI: 74737
Homologene: 32282
Hdac5
Name: histone deacetylase 5
Synonyms: mHDA1
Type: Gene
Species: Mouse
Chromosome: 11
NCBI: 15184
Homologene: 3995
Slc25a13
Name: solute carrier family 25 (mitochondrial carrier, adenine nucleotide translocator), member 13
Synonyms: Ctrn, citrin
Type: Gene
Species: Mouse
Chromosome: 6
NCBI: 50799
Homologene: 22800
Rexo1
Name: REX1, RNA exonuclease 1
Synonyms: 1700021P10Rik, 2610511M11Rik, Tceb3bp1, Rex1
Type: Gene
Species: Mouse
Chromosome: 10
NCBI: 66932
Homologene: 41391
Wac
Name: WW domain containing adaptor with coiled-coil
Synonyms: Wwp4, 1110067P07Rik, A230035H12Rik
Type: Gene
Species: Mouse
Chromosome: 18
NCBI: 225131
Homologene: 41148
Genetic Alterations
ENU-induced transitions at the following base pair locations (GRCm38):
  • A to T, chromosome 1 at 46,515,439 bp
  • C to A, chromosome 1 at 46,649,340 bp
  • A to G, chromosome 1 at 46,649,351 bp
  • A to G, chromosome 2 at 111,329,112 bp
  • T to A, chromosome 3 at 62,488,917 bp
  • T to A, chromosome 3 at 122,426,633 bp
  • G to T, chromosome 4 at 43,682,249 bp
  • C to T, chromosome 5 at 31,036,830 bp
  • C to A, chromosome 5 at 110,277,707 bp
  • C to T, chromosome 6 at 6,073,454 bp
  • T to C, chromosome 6 at 56,753,885 bp
  • T to C, chromosome 6 at 85,478,523 bp
  • A to G, chromosome 6 at 116,405,663 bp
  • A to G, chromosome 7 at 16,917,583 bp
  • T to A, chromosome 7 at 55,979,823 bp
  • T to C, chromosome 7 at 65,678,142 bp
  • A to T, chromosome 7 at 66,281,648 bp
  • A to G, chromosome 7 at 86,526,912 bp
  • T to A, chromosome 7 at 92,657,882 bp
  • T to C, chromosome 7 at 102,781,787 bp
  • T to C, chromosome 8 at 18,933,553 bp
  • T to C, chromosome 8 at 45,248,774 bp
  • A to G, chromosome 8 at 70,242,770 bp
  • A to C, chromosome 8 at 95,545,992 bp
  • CT to C, chromosome 8 at 104,182,032 bp
  • T to G, chromosome 9 at 18,647,188 bp
  • A to G, chromosome 9 at 19,584,989 bp
  • A to G, chromosome 9 at 73,930,608 bp
  • A to T, chromosome 9 at 96,686,654 bp
  • T to C, chromosome 10 at 5,301,340 bp
  • C to T, chromosome 10 at 80,544,014 bp
  • A to T, chromosome 10 at 86,044,534 bp
  • T to C, chromosome 10 at 93,846,489 bp
  • T to G, chromosome 10 at 127,884,622 bp
  • T to A, chromosome 11 at 29,578,149 bp
  • C to A, chromosome 11 at 102,197,056 bp
  • T to C, chromosome 11 at 116,853,079 bp
  • C to T, chromosome 12 at 84,813,317 bp
  • A to T, chromosome 12 at 104,151,721 bp
  • T to G, chromosome 13 at 63,539,830 bp
  • T to A, chromosome 13 at 105,112,485 bp
  • A to G, chromosome 14 at 61,208,934 bp
  • C to T, chromosome 15 at 85,902,114 bp
  • A to G, chromosome 15 at 101,436,562 bp
  • T to G, chromosome 16 at 26,960,528 bp
  • G to A, chromosome 17 at 37,111,602 bp
  • C to T, chromosome 17 at 67,353,956 bp
  • C to A, chromosome 18 at 7,868,884 bp
  • T to A, chromosome 18 at 20,032,519 bp
  • T to G, chromosome 18 at 57,898,128 bp
  • G to A, chromosome 19 at 12,824,799 bp
Phenotype
G1 male heterozygous mice are viable and fertile. The G1 mice were not characterized prior to archiving.
Strain GQC Summary
Gene Specific Genotyping:

To request gene-specific and other genotyping services for a strain, please contact the distribution MMRRC Center for more information.

Background Genetic Quality:

The MMRRC has developed a Genetic Quality Control pipeline using the MiniMUGA array to provide additional information to identify and validate genetic backgrounds of MMRRC strains. For more information on whether genetic background data is available, please contact MMRRC_GeneticQC@med.unc.edu. Note: that MiniMUGA genetic background data is not available on all strains, but can be ordered if desired.

Suggested Control Mice
Littermates of all relevant genotypes.
Donor
Bruce Beutler, M.D., University of Texas Southwestern Medical Center.
Primary Reference
MUTAGENETIX record for R6615 (G1). B. Beutler and colleagues, Center for the Genetics of Host Defense, UT Southwestern, Dallas, TX.
Strain Development
C57BL/6J mice were injected with ENU, resulting in G0 mice that were bred back to C57BL/6J. Sperm was archived from G1 male mice that produced viable G2 mice.


Disclaimer: If MMRRC Strain Genetic Quality Control (GQC; based on MiniMUGA genotyping and analysis) has been completed for this strain, the information might differ from the genetic background information provided by the submitter. MiniMUGA genetic analysis is done on a strain’s tissue samples taken when archived by or ordered from the assigned MMRRC Center.

Colony and Husbandry Information

Colony Surveillance Program and Current Health Reports

Mice recovered from a cryo-archive will have health surveillance performed on recipient females. Health reports will be provided prior to shipment. If you require additional health status information, please email mmrrc@missouri.edu.
Coat Color
Black
MMRRC Breeding System
Random intra-strain mating
Breeding Scheme(s)
When maintaining a live colony, heterozygous mice may be bred together, bred with wild-type siblings, or bred with C57BL/6J inbred mice.
Overall Breeding Performance
Undetermined; during development, mating was done with heterozygous males; viability, fertility and other breeding statistics for heterozygous females and homozygotes are unverified, but presumably would be similar to the C57BL/6J background strain
Average litter size
Undetermined
Recommended wean age
3 weeks

Order Information

Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.

Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.

The donor or their institution limits the distribution to non-profit institutions only.

Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.

Click button to Request this one strain. (Use the MMRRC Catalog Search to request more than one strain.)
MMRRC Item # Description Distribution Fee / Unit (US $)
*Shipping & Handling not included*
Units Notes
044738-MU-RESUS Litter recovered from cryo-archive $5,475.00 / Non-Profit Litter Recovered litter4; additional fees for any special requests.
Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

1 The distribution fee covers the expense of rederiving mice from a live mouse; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

2 An aliquot contains a sufficient number of embryos (in one or more vials or straws and based on the transfer success rate of the MMRRC facility) to transfer into one to three recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.

3 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.

4 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.