Strain Name:
C57BL/6J-MtgxR6646Btlr/Mmmh
Stock Number:
044767-MU
Citation ID:
RRID:MMRRC_044767-MU
Other Names:
R6646 (G1)
Major Collection:

Strain Information

Dst
Name: dystonin
Synonyms: Macf2, A830042E19Rik, Bpag1, Bpag, bullous pemphigoid antigen 1, nmf203, athetoid, nmf339, BPAG1, BPAG1-n, 2310001O04Rik, bullous pemphigoid antigen 1, ah
Type: Gene
Species: Mouse
Chromosome: 1
NCBI: 13518
HGNC: HGNC:1090
Homologene: 136716
Ehmt1
Name: euchromatic histone methyltransferase 1
Synonyms: 9230102N17Rik, KMT1D
Type: Gene
Species: Mouse
Chromosome: 2
NCBI: 77683
Homologene: 11698
Rpap1
Name: RNA polymerase II associated protein 1
Synonyms: 1190005L06Rik, A730023M06Rik
Type: Gene
Species: Mouse
Chromosome: 2
NCBI: 68925
Homologene: 32269
Ptprg
Name: protein tyrosine phosphatase receptor type G
Synonyms: 5430405N12Rik, RPTPgamma
Type: Gene
Species: Mouse
Chromosome: 14
NCBI: 19270
HGNC: HGNC:9671
Homologene: 2129
Sorbs1
Name: sorbin and SH3 domain containing 1
Synonyms: Sh3d5, Ponsin, c-Cbl-associated protein, CAP, 9530001P15Rik, 2310065E01Rik
Type: Gene
Species: Mouse
Chromosome: 19
NCBI: 20411
VEGA: 19
Homologene: 83252
Msh6
Name: mutS homolog 6
Synonyms: GTBP, Msh6, Gtmbp
Type: Gene
Species: Mouse
Chromosome: 17
NCBI: 17688
VEGA: 17
HGNC: HGNC:7329
Homologene: 149
Crebbp
Name: CREB binding protein
Synonyms: KAT3A, CBP
Type: Gene
Species: Mouse
Chromosome: 16
NCBI: 12914
HGNC: HGNC:2348
Homologene: 68393
Doc2a
Name: double C2, alpha
Type: Gene
Species: Mouse
Chromosome: 7
NCBI: 13446
HGNC: HGNC:2985
Homologene: 2657
Lrrn2
Name: leucine rich repeat protein 2, neuronal
Synonyms: 5730406J09Rik, NLRR-2
Type: Gene
Species: Mouse
Chromosome: 1
NCBI: 16980
Homologene: 21274
Plaa
Name: phospholipase A2, activating protein
Synonyms: D4Ertd618e, Ufd3
Type: Gene
Species: Mouse
Chromosome: 4
NCBI: 18786
HGNC: HGNC:9043
Homologene: 3138
Dedd2
Name: death effector domain-containing DNA binding protein 2
Synonyms: 2410050E11Rik, FLAME-3
Type: Gene
Species: Mouse
Chromosome: 7
NCBI: 67379
Homologene: 23569
Wdr75
Name: WD repeat domain 75
Synonyms: 2410118I19Rik, 1300003A18Rik
Type: Gene
Species: Mouse
Chromosome: 1
NCBI: 73674
Homologene: 32771
Slc16a6
Name: solute carrier family 16 (monocarboxylic acid transporters), member 6
Type: Gene
Species: Mouse
Chromosome: 11
NCBI: 104681
Homologene: 20984
Gipc2
Name: GIPC PDZ domain containing family, member 2
Synonyms: 2200002N01Rik, Semcap2
Type: Gene
Species: Mouse
Chromosome: 3
NCBI: 54120
Homologene: 22994
Arhgef28
Name: Rho guanine nucleotide exchange factor 28
Synonyms: D13Bwg1089e, Rho specific exchange factor, 9230110L08Rik, RIP2, Rgnef, p190RhoGEF, RhoGEF
Type: Gene
Species: Mouse
Chromosome: 13
NCBI: 110596
VEGA: 13
Homologene: 8078
Dennd6b
Name: DENN domain containing 6B
Synonyms: Fam116b, 1700027J05Rik
Type: Gene
Species: Mouse
Chromosome: 15
NCBI: 69440
VEGA: 15
Homologene: 57065
Carmil3
Name: capping protein regulator and myosin 1 linker 3
Synonyms: Lrrc16b
Type: Gene
Species: Mouse
Chromosome: 14
NCBI: 268747
VEGA: 14
Homologene: 74564
Skint5
Name: selection and upkeep of intraepithelial T cells 5
Synonyms: OTTMUSG00000008560
Type: Gene
Species: Mouse
Chromosome: 4
NCBI: 242627
Homologene: 135888
Synm
Name: synemin, intermediate filament protein
Synonyms: Synemin, 4930412K21Rik, Dmn
Type: Gene
Species: Mouse
Chromosome: 7
NCBI: 233335
Homologene: 9081
Obscn
Name: obscurin, cytoskeletal calmodulin and titin-interacting RhoGEF
Synonyms: OTTMUSG00000005786, LOC380698
Type: Gene
Species: Mouse
Chromosome: 11
NCBI: 380698
Homologene: 70869
Pard3b
Name: par-3 family cell polarity regulator beta
Synonyms: PAR3beta, Als2cr19, PAR3L, 1810008K04Rik, PAR3B, 2010002N16Rik, 2810455B10Rik
Type: Gene
Species: Mouse
Chromosome: 1
NCBI: 72823
Homologene: 35389
Ada
Name: adenosine deaminase
Type: Gene
Species: Mouse
Chromosome: 2
NCBI: 11486
HGNC: HGNC:186
Homologene: 37249
Nebl
Name: nebulette
Synonyms: D830029A09Rik, A630080F05Rik, 1200007O21Rik, Lnebl
Type: Gene
Species: Mouse
Chromosome: 2
NCBI: 74103
Homologene: 128431
Spata31h1
Name: SPATA31 subfamily H member 1
Synonyms: 4932415D10Rik
Type: Gene
Species: Mouse
Chromosome: 10
NCBI: 102635990
VEGA: 10
Homologene: 82476
Plxnb1
Name: plexin B1
Synonyms: 2900002G15Rik
Type: Gene
Species: Mouse
Chromosome: 9
NCBI: 235611
HGNC: HGNC:9103
Homologene: 130508
Aldh1a7
Name: aldehyde dehydrogenase family 1, subfamily A7
Synonyms: Ahd2-like, Aldh-pb
Type: Gene
Species: Mouse
Chromosome: 19
NCBI: 26358
HGNC: HGNC:402
Homologene: 137245
Pcdhb7
Name: protocadherin beta 7
Synonyms: Pcdhb4B, PcdhbG
Type: Gene
Species: Mouse
Chromosome: 18
NCBI: 93878
HGNC: HGNC:8689
Homologene: 87123
Vmn2r99
Name: vomeronasal 2, receptor 99
Synonyms: EG665376
Type: Gene
Species: Mouse
Chromosome: 17
NCBI: 665376
Homologene: 115024
Plcd1
Name: phospholipase C, delta 1
Synonyms: PLC-delta 1
Type: Gene
Species: Mouse
Chromosome: 9
NCBI: 18799
VEGA: 9
HGNC: HGNC:9060
Homologene: 21252
Tcerg1l
Name: transcription elongation regulator 1-like
Synonyms: 5730476P14Rik
Type: Gene
Species: Mouse
Chromosome: 7
NCBI: 70571
Homologene: 52165
Gbp9
Name: guanylate-binding protein 9
Type: Gene
Species: Mouse
Chromosome: 5
NCBI: 236573
Homologene: 128731
Krt6b
Name: keratin 6B
Synonyms: Krt2-6b, mK6[b]
Type: Gene
Species: Mouse
Chromosome: 15
NCBI: 16688
VEGA: 15
Homologene: 136794
Zswim2
Name: zinc finger SWIM-type containing 2
Synonyms: 1700025P14Rik, MEX, 4933437F18Rik
Type: Gene
Species: Mouse
Chromosome: 2
NCBI: 71861
Homologene: 32689
Scx
Name: scleraxis scleraxis bHLH transcription factor
Synonyms: bHLHa41, Scl
Type: Gene
Species: Mouse
Chromosome: 15
NCBI: 20289
VEGA: 15
Homologene: 85195
Rela
Name: v-rel reticuloendotheliosis viral oncogene homolog A (avian)
Synonyms: p65 NF kappaB, p65
Type: Gene
Species: Mouse
Chromosome: 19
NCBI: 19697
HGNC: HGNC:9955
Homologene: 32064
Loxl4
Name: lysyl oxidase-like 4
Synonyms: 4833426I20Rik
Type: Gene
Species: Mouse
Chromosome: 19
NCBI: 67573
Homologene: 12977
Dnase1l1
Name: deoxyribonuclease 1-like 1
Synonyms: Dnl1ll, G4.8, Dnase1ll, 2310005K03Rik
Type: Gene
Species: Mouse
Chromosome: X
NCBI: 69537
HGNC: HGNC:2957
Homologene: 4896
Rpl15
Name: ribosomal protein L15
Synonyms: 2510008H07Rik
Type: Gene
Species: Mouse
Chromosome: 14
NCBI: 66480
Homologene: 37713
Genetic Alterations
ENU-induced transitions at the following base pair locations (GRCm38):
  • A to G, chromosome 1 at 34,268,807 bp
  • T to A, chromosome 1 at 45,799,087 bp
  • T to C, chromosome 1 at 62,161,121 bp
  • C to T, chromosome 1 at 132,939,056 bp
  • T to C, chromosome 2 at 17,376,685 bp
  • A to T, chromosome 2 at 24,806,310 bp
  • T to A, chromosome 2 at 83,915,784 bp
  • T to C, chromosome 2 at 119,780,131 bp
  • G to T, chromosome 2 at 163,735,423 bp
  • A to T, chromosome 3 at 152,094,201 bp
  • A to T, chromosome 4 at 94,589,978 bp
  • T to A, chromosome 4 at 113,940,777 bp
  • A to G, chromosome 5 at 105,082,903 bp
  • T to C, chromosome 7 at 25,203,613 bp
  • A to T, chromosome 7 at 67,735,127 bp
  • G to A, chromosome 7 at 126,851,619 bp
  • A to T, chromosome 7 at 138,395,183 bp
  • C to A, chromosome 9 at 109,108,827 bp
  • T to A, chromosome 9 at 119,075,032 bp
  • T to A, chromosome 10 at 82,296,830 bp
  • T to C, chromosome 11 at 59,082,718 bp
  • C to T, chromosome 11 at 109,453,162 bp
  • A to G, chromosome 13 at 97,939,494 bp
  • C to A, chromosome 14 at 11,962,714 bp
  • T to C, chromosome 14 at 18,270,040 bp
  • A to T, chromosome 14 at 55,507,930 bp
  • C to A, chromosome 15 at 76,457,921 bp
  • G to T, chromosome 15 at 89,186,184 bp
  • T to A, chromosome 15 at 101,677,214 bp
  • C to T, chromosome 16 at 4,119,806 bp
  • T to G, chromosome 17 at 19,380,031 bp
  • T to C, chromosome 17 at 87,986,442 bp
  • T to A, chromosome 18 at 37,343,974 bp
  • T to G, chromosome 19 at 5,647,104 bp
  • C to T, chromosome 19 at 20,699,911 bp
  • C to T, chromosome 19 at 40,325,549 bp
  • T to C, chromosome 19 at 42,598,781 bp
  • C to T, chromosome X at 74,277,038 bp
Phenotype
G1 male heterozygous mice are viable and fertile. The G1 mice were not characterized prior to archiving.
Strain Development
C57BL/6J mice were injected with ENU, resulting in G0 mice that were bred back to C57BL/6J. Sperm was archived from G1 male mice that produced viable G2 mice.
Suggested Control Mice
Wild-type littermates or C57BL/6J mice
MMRRC Genetic QC Summary
The MMRRC Centers have developed a genetic QC pipeline using MiniMUGA array genotyping to provide additional information on strain backgrounds for MMRRC congenic and inbred strains. For more information on when data may be available, or to request genotyping for a strain of interest, please contact mmrrc@missouri.edu. Older strains may not have this information.
Donor
Bruce Beutler, M.D., University of Texas Southwestern Medical Center.
Primary Reference
MUTAGENETIX record for R6646 (G1). B. Beutler and colleagues, Center for the Genetics of Host Defense, UT Southwestern, Dallas, TX.

Colony and Husbandry Information

Colony Surveillance Program and Current Health Reports

Mice recovered from a cryo-archive will have health surveillance performed on recipient females. Health reports will be provided prior to shipment. If you require additional health status information, please email mmrrc@missouri.edu.
Coat Color
Black
MMRRC Breeding System
Random intra-strain mating
Breeding Scheme(s)
When maintaining a live colony, heterozygous mice may be bred together, bred with wild-type siblings, or bred with C57BL/6J inbred mice.
Overall Breeding Performance
Undetermined; during development, mating was done with heterozygous males; viability, fertility and other breeding statistics for heterozygous females and homozygotes are unverified, but presumably would be similar to the C57BL/6J background strain
Average litter size
Undetermined
Recommended wean age
3 weeks

Order Request Information

Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.

Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.

The donor or their institution limits the distribution to non-profit institutions only.

Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.

Click button to Request this one strain. (Use the MMRRC Catalog Search to request more than one strain.)
MMRRC Item # Description Distribution Fee / Unit (US $)
*Shipping & Handling not included*
Units Notes
044767-MU-RESUS Litter recovered from cryo-archive $5,475.00 / Non-Profit Litter Recovered litter4; additional fees for any special requests.
Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

1 The distribution fee covers the expense of rederiving mice from a live mouse; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

2 An aliquot contains a sufficient number of embryos (in one or more vials or straws and based on the transfer success rate of the MMRRC facility) to transfer into one to three recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.

3 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.

4 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.