Strain Name:
C57BL/6J-MtgxR6655Btlr/Mmmh
Stock Number:
044776-MU
Citation ID:
RRID:MMRRC_044776-MU
Other Names:
R6655 (G1)
Major Collection:

Strain Information

Dlc1
Name: deleted in liver cancer 1
Synonyms: p122-RhoGAP, STARD12, Arhgap7, A730069N07Rik
Type: Gene
Species: Mouse
Chromosome: 8
NCBI: 50768
HGNC: HGNC:2897
Homologene: 4442
Lrp2
Name: low density lipoprotein receptor-related protein 2
Synonyms: b2b1625.2Clo, Gp330, Megalin, D230004K18Rik
Type: Gene
Species: Mouse
Chromosome: 2
NCBI: 14725
HGNC: HGNC:6694
Homologene: 20952
Dscaml1
Name: DS cell adhesion molecule like 1
Synonyms: 4930435C18Rik, 4921507G06Rik
Type: Gene
Species: Mouse
Chromosome: 9
NCBI: 114873
VEGA: 9
Homologene: 79549
Neurl4
Name: neuralized E3 ubiquitin protein ligase 4
Synonyms: 0610025P10Rik
Type: Gene
Species: Mouse
Chromosome: 11
NCBI: 216860
Homologene: 13029
Rab3gap2
Name: RAB3 GTPase activating protein subunit 2
Synonyms: 1110059F07Rik
Type: Gene
Species: Mouse
Chromosome: 1
NCBI: 98732
Homologene: 40842
Nbn
Name: nibrin
Synonyms: Nbs1
Type: Gene
Species: Mouse
Chromosome: 4
NCBI: 27354
HGNC: HGNC:7652
Homologene: 1858
Fkbp8
Name: FK506 binding protein 8
Synonyms: 38kDa, Fkbp38
Type: Gene
Species: Mouse
Chromosome: 8
NCBI: 14232
HGNC: HGNC:3724
Homologene: 7720
Sec22b
Name: SEC22 homolog B, vesicle trafficking protein
Synonyms: ERS-24, 4930564D15Rik, Sec22l1
Type: Gene
Species: Mouse
Chromosome: 3
NCBI: 20333
Homologene: 3597
Nol8
Name: nucleolar protein 8
Synonyms: D13Ertd548e, 5730412B09Rik, 4921532D18Rik
Type: Gene
Species: Mouse
Chromosome: 13
NCBI: 70930
VEGA: 13
Homologene: 41210
Wnt16
Name: wingless-type MMTV integration site family, member 16
Synonyms: E130309I19Rik
Type: Gene
Species: Mouse
Chromosome: 6
NCBI: 93735
Homologene: 62175
Arhgef28
Name: Rho guanine nucleotide exchange factor 28
Synonyms: Rho specific exchange factor, RIP2, Rgnef, p190RhoGEF, 9230110L08Rik, D13Bwg1089e, RhoGEF
Type: Gene
Species: Mouse
Chromosome: 13
NCBI: 110596
VEGA: 13
Homologene: 8078
Myof
Name: myoferlin
Synonyms: E030042N20Rik, Fer1l3, 2310051D19Rik
Type: Gene
Species: Mouse
Chromosome: 19
NCBI: 226101
VEGA: 19
HGNC: HGNC:3656
Homologene: 40882
AAdacl4fm3
Name: AADACL4 family member 3
Synonyms: Gm13178
Type: Gene
Species: Mouse
Chromosome: 4
NCBI: 546849
Homologene: 82586
Vmn2r111
Name: vomeronasal 2, receptor 111
Synonyms: EG210876
Type: Gene
Species: Mouse
Chromosome: 17
NCBI: 210876
Homologene: 86604
Cd109
Name: CD109 antigen
Synonyms: Gov platelet alloantigens, 9930012E15Rik
Type: Gene
Species: Mouse
Chromosome: 9
NCBI: 235505
Homologene: 25183
Ttll9
Name: tubulin tyrosine ligase-like family, member 9
Synonyms: 4930509O20Rik, 1700016F23Rik
Type: Gene
Species: Mouse
Chromosome: 2
NCBI: 74711
Homologene: 41769
Shank1
Name: SH3 and multiple ankyrin repeat domains 1
Type: Gene
Species: Mouse
Chromosome: 7
NCBI: 243961
Homologene: 22949
Samd9l
Name: sterile alpha motif domain containing 9-like
Synonyms: ESTM25
Type: Gene
Species: Mouse
Chromosome: 6
NCBI: 209086
HGNC: HGNC:1349
Homologene: 7707
Vmn1r222
Name: vomeronasal 1 receptor 222
Synonyms: V1rh16
Type: Gene
Species: Mouse
Chromosome: 13
NCBI: 171274
Homologene: 110880
Ssbp2
Name: single-stranded DNA binding protein 2
Synonyms: Ssdp2, Hspc116, 1500004K09Rik, A830008M03Rik, 9330163K02Rik, 2310079I02Rik
Type: Gene
Species: Mouse
Chromosome: 13
NCBI: 66970
Homologene: 22724
Kcnh1
Name: potassium voltage-gated channel, subfamily H (eag-related), member 1
Synonyms: ether a go-go, Kv10.1, Eag1
Type: Gene
Species: Mouse
Chromosome: 1
NCBI: 16510
HGNC: HGNC:6250
Homologene: 68242
Pex26
Name: peroxisomal biogenesis factor 26
Synonyms: peroxisome biogenesis factor 26, 4632428M11Rik
Type: Gene
Species: Mouse
Chromosome: 6
NCBI: 74043
Homologene: 9922
Veph1
Name: ventricular zone expressed PH domain-containing 1
Synonyms: Veph, 2810471M23Rik
Type: Gene
Species: Mouse
Chromosome: 3
NCBI: 72789
Homologene: 11624
Akr1b1
Name: aldo-keto reductase family 1 member B
Synonyms: Ahr-1, AR, Aldor1, Aldr1, Ahr1, ALR2, Akr1b3
Type: Gene
Species: Mouse
Chromosome: 6
NCBI: 11677
HGNC: HGNC:381
Homologene: 133743
Or2t46
Name: olfactory receptor family 2 subfamily T member 46
Synonyms: MOR275-11_p, MOR275-5, GA_x6K02T2NKPP-844642-843680, Olfr325
Type: Gene
Species: Mouse
Chromosome: 11
NCBI: 258261
Homologene: 133015
Alg3
Name: ALG3 alpha-1,3- mannosyltransferase
Synonyms: D16Ertd36e
Type: Gene
Species: Mouse
Chromosome: 16
NCBI: 208624
Homologene: 4228
Zfp747l1
Name: zinc finger protein 747 like 1
Synonyms: 9130019O22Rik
Type: Gene
Species: Mouse
Chromosome: 7
NCBI: 78921
Homologene: 118005
Or6c213
Name: olfactory receptor family 6 subfamily C member 213
Synonyms: MOR110-5, GA_x6K02T2PULF-11417610-11416669, MOR110-12, Olfr806
Type: Gene
Species: Mouse
Chromosome: 10
NCBI: 258546
Homologene: 73936
Gm5160
Name: predicted gene 5160
Type: Gene
Species: Mouse
Chromosome: 18
NCBI: 382096
VEGA: 18
Genetic Alterations
ENU-induced transitions at the following base pair locations (GRCm38):
  • T to C, chromosome 1 at 185,250,011 bp
  • A to G, chromosome 1 at 192,413,083 bp
  • A to G, chromosome 2 at 69,453,858 bp
  • T to C, chromosome 2 at 153,000,303 bp
  • T to A, chromosome 3 at 66,205,613 bp
  • T to A, chromosome 3 at 97,914,648 bp
  • A to G, chromosome 4 at 15,981,696 bp
  • T to A, chromosome 4 at 144,705,245 bp
  • C to A, chromosome 6 at 3,377,247 bp
  • T to C, chromosome 6 at 22,290,966 bp
  • C to T, chromosome 6 at 34,310,004 bp
  • T to C, chromosome 6 at 121,190,211 bp
  • A to G, chromosome 7 at 44,327,220 bp
  • A to G, chromosome 7 at 127,384,340 bp
  • A to G, chromosome 8 at 36,572,716 bp
  • A to G, chromosome 8 at 70,532,670 bp
  • G to A, chromosome 9 at 45,746,937 bp
  • A to G, chromosome 9 at 78,684,938 bp
  • T to C, chromosome 10 at 129,738,087 bp
  • T to A, chromosome 11 at 58,581,210 bp
  • T to C, chromosome 11 at 69,910,916 bp
  • A to C, chromosome 13 at 23,232,716 bp
  • T to C, chromosome 13 at 49,654,392 bp
  • C to T, chromosome 13 at 91,664,149 bp
  • A to G, chromosome 13 at 97,899,655 bp
  • T to A, chromosome 16 at 20,609,026 bp
  • T to C, chromosome 17 at 22,559,051 bp
  • T to C, chromosome 18 at 14,425,130 bp
  • T to C, chromosome 19 at 37,934,791 bp
Phenotype
G1 male heterozygous mice are viable and fertile. The G1 mice were not characterized prior to archiving.
Strain Development
C57BL/6J mice were injected with ENU, resulting in G0 mice that were bred back to C57BL/6J. Sperm was archived from G1 male mice that produced viable G2 mice.
Suggested Control Mice
Wild-type littermates or C57BL/6J mice
MMRRC Genetic QC Summary
The MMRRC Centers have developed a genetic QC pipeline using MiniMUGA array genotyping to provide additional information on strain backgrounds for MMRRC congenic and inbred strains. For more information on when data may be available, or to request genotyping for a strain of interest, please contact mmrrc@missouri.edu. Older strains may not have this information.
Donor
Bruce Beutler, M.D., University of Texas Southwestern Medical Center.
Primary Reference
MUTAGENETIX record for R6655 (G1). B. Beutler and colleagues, Center for the Genetics of Host Defense, UT Southwestern, Dallas, TX.

Colony and Husbandry Information

Colony Surveillance Program and Current Health Reports

Mice recovered from a cryo-archive will have health surveillance performed on recipient females. Health reports will be provided prior to shipment. If you require additional health status information, please email mmrrc@missouri.edu.
Coat Color
Black
MMRRC Breeding System
Random intra-strain mating
Breeding Scheme(s)
When maintaining a live colony, heterozygous mice may be bred together, bred with wild-type siblings, or bred with C57BL/6J inbred mice.
Overall Breeding Performance
Undetermined; during development, mating was done with heterozygous males; viability, fertility and other breeding statistics for heterozygous females and homozygotes are unverified, but presumably would be similar to the C57BL/6J background strain
Average litter size
Undetermined
Recommended wean age
3 weeks

Order Request Information

Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.

Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.

The donor or their institution limits the distribution to non-profit institutions only.

Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.

Click button to Request this one strain. (Use the MMRRC Catalog Search to request more than one strain.)
MMRRC Item # Description Distribution Fee / Unit (US $)
*Shipping & Handling not included*
Units Notes
044776-MU-RESUS Litter recovered from cryo-archive $5,475.00 / Non-Profit Litter Recovered litter4; additional fees for any special requests.
Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

1 The distribution fee covers the expense of rederiving mice from a live mouse; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

2 An aliquot contains a sufficient number of embryos (in one or more vials or straws and based on the transfer success rate of the MMRRC facility) to transfer into one to three recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.

3 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.

4 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.