Strain Name:
C57BL/6J-MtgxR6663Btlr/Mmmh
Stock Number:
044783-MU
Citation ID:
RRID:MMRRC_044783-MU
Other Names:
R6663 (G1)
Major Collection:

Strain Information

Pln
Name: phospholamban
Synonyms: PLB
Type: Gene
Species: Mouse
Chromosome: 10
NCBI: 18821
VEGA: 10
HGNC: HGNC:9080
Homologene: 2002
Lyst
Name: lysosomal trafficking regulator
Synonyms: D13Sfk13
Type: Gene
Species: Mouse
Chromosome: 13
NCBI: 17101
VEGA: 13
HGNC: HGNC:1968
Homologene: 61
Map1b
Name: microtubule-associated protein 1B
Synonyms: Mtap5, Mtap1b, Mtap-5, MAP5, LC1
Type: Gene
Species: Mouse
Chromosome: 13
NCBI: 17755
VEGA: 13
HGNC: HGNC:6836
Homologene: 38111
Ankrd17
Name: ankyrin repeat domain 17
Synonyms: 4933425K22Rik, Gtar, A130069E23Rik
Type: Gene
Species: Mouse
Chromosome: 5
NCBI: 81702
Homologene: 82403
Zfp462
Name: zinc finger protein 462
Synonyms: Zfpip, 9430078C22Rik, Gt4-2
Type: Gene
Species: Mouse
Chromosome: 4
NCBI: 242466
Homologene: 41430
Cux1
Name: cut-like homeobox 1
Synonyms: Cux, Cux-1, Cutl1, CDP
Type: Gene
Species: Mouse
Chromosome: 5
NCBI: 13047
HGNC: HGNC:2557
Plekha5
Name: pleckstrin homology domain containing, family A member 5
Synonyms: 2810431N21Rik, PEPP2
Type: Gene
Species: Mouse
Chromosome: 6
NCBI: 109135
Homologene: 10377
Mark3
Name: MAP/microtubule affinity regulating kinase 3
Synonyms: 1600015G02Rik, A430080F22Rik, ETK-1, C-TAK1
Type: Gene
Species: Mouse
Chromosome: 12
NCBI: 17169
VEGA: 12
HGNC: HGNC:6897
Homologene: 55653
Dbf4
Name: DBF4 zinc finger
Synonyms: Ask
Type: Gene
Species: Mouse
Chromosome: 5
NCBI: 27214
Homologene: 40892
Cpsf2
Name: cleavage and polyadenylation specific factor 2
Synonyms: 2610024B04Rik, 100kDa, Cpsf
Type: Gene
Species: Mouse
Chromosome: 12
NCBI: 51786
VEGA: 12
HGNC: HGNC:2325
Homologene: 6460
Fezf1
Name: Fez family zinc finger 1
Synonyms: 3110069A13Rik, Fez, Zfp312-like
Type: Gene
Species: Mouse
Chromosome: 6
NCBI: 73191
Homologene: 19252
Kifc1
Name: kinesin family member C1
Synonyms: Tctex7a, Tctex7, Tctex-7, kinesin family c-terminal 5A, KNSL2, Gm4137, Knsl2a, HSET, Tctex-7A, Kifc5a
Type: Gene
Species: Mouse
Chromosome: 17
NCBI: 100502766
HGNC: HGNC:6389
Homologene: 83229
Med6
Name: mediator complex subunit 6
Synonyms: 1500012F11Rik
Type: Gene
Species: Mouse
Chromosome: 12
NCBI: 69792
Homologene: 3990
Zfp668
Name: zinc finger protein 668
Synonyms: E130018B19Rik
Type: Gene
Species: Mouse
Chromosome: 7
NCBI: 244219
Homologene: 11667
Phf10
Name: PHD finger protein 10
Synonyms: Baf45a, 1810055P05Rik
Type: Gene
Species: Mouse
Chromosome: 17
NCBI: 72057
Homologene: 10112
Dnhd1
Name: dynein heavy chain domain 1
Synonyms: 8030491N06Rik
Type: Gene
Species: Mouse
Chromosome: 7
NCBI: 77505
Homologene: 131117
Tbccd1
Name: TBCC domain containing 1
Synonyms: 5730478M09Rik
Type: Gene
Species: Mouse
Chromosome: 16
NCBI: 70573
VEGA: 16
Homologene: 32392
Clcn2
Name: chloride channel, voltage-sensitive 2
Synonyms: ClC-2, Clc2, nmf240
Type: Gene
Species: Mouse
Chromosome: 16
NCBI: 12724
HGNC: HGNC:2020
Homologene: 3213
Mroh2b
Name: maestro heat-like repeat family member 2B
Synonyms: Heatr7b2, 4930455B06Rik
Type: Gene
Species: Mouse
Chromosome: 15
NCBI: 223825
VEGA: 15
Homologene: 128807
Cyp2j9
Name: cytochrome P450, family 2, subfamily j, polypeptide 9
Synonyms: 8430417E17Rik
Type: Gene
Species: Mouse
Chromosome: 4
NCBI: 74519
HGNC: HGNC:2634
Homologene: 137388
Prg4
Name: proteoglycan 4 (megakaryocyte stimulating factor, articular superficial zone protein)
Synonyms: MSF, lubricin, SZP, DOL54
Type: Gene
Species: Mouse
Chromosome: 1
NCBI: 96875
HGNC: HGNC:9364
Homologene: 137352
Col22a1
Name: collagen, type XXII, alpha 1
Synonyms: C80743, 2310067L16Rik
Type: Gene
Species: Mouse
Chromosome: 15
NCBI: 69700
Homologene: 43567
Itga1
Name: integrin alpha 1
Synonyms: Vla1, CD49A, E130012M19Rik
Type: Gene
Species: Mouse
Chromosome: 13
NCBI: 109700
VEGA: 13
HGNC: HGNC:6134
Homologene: 57137
Zup1
Name: zinc finger containing ubiquitin peptidase 1
Synonyms: 2700019D07Rik, Zufsp
Type: Gene
Species: Mouse
Chromosome: 10
NCBI: 72580
VEGA: 10
Homologene: 12472
Ube2j1
Name: ubiquitin-conjugating enzyme E2J 1
Synonyms: 0710008M05Rik, 1110030I22Rik, Ncube, Ubc6p
Type: Gene
Species: Mouse
Chromosome: 4
NCBI: 56228
Homologene: 41090
Lrrc14b
Name: leucine rich repeat containing 14B
Type: Gene
Species: Mouse
Chromosome: 13
NCBI: 432779
Homologene: 47758
Mfhas1
Name: malignant fibrous histiocytoma amplified sequence 1
Synonyms: 2310066G09Rik, D8Ertd91e
Type: Gene
Species: Mouse
Chromosome: 8
NCBI: 52065
Homologene: 3114
Irx2
Name: Iroquois homeobox 2
Synonyms: IRX6
Type: Gene
Species: Mouse
Chromosome: 13
NCBI: 16372
Homologene: 56490
Slc35b3
Name: solute carrier family 35, member B3
Synonyms: 4921526O06Rik, PAPST2
Type: Gene
Species: Mouse
Chromosome: 13
NCBI: 108652
VEGA: 13
Homologene: 9313
Nkx2-9
Name: NK2 homeobox 9
Synonyms: Nkx2.9, Nkx-2.9, tinman
Type: Gene
Species: Mouse
Chromosome: 12
NCBI: 18094
Homologene: 7444
Or4p20
Name: olfactory receptor family 4 subfamily P member 20
Synonyms: GA_x6K02T2Q125-49911636-49910701, Olfr1181, MOR225-9P
Type: Gene
Species: Mouse
Chromosome: 2
NCBI: 258060
Homologene: 86666
Genetic Alterations
ENU-induced transitions at the following base pair locations (GRCm38):
  • G to A, chromosome 1 at 150,455,101 bp
  • T to A, chromosome 2 at 88,423,350 bp
  • T to A, chromosome 2 at 138,279,083 bp
  • C to T, chromosome 4 at 33,045,198 bp
  • A to G, chromosome 4 at 55,008,933 bp
  • C to A, chromosome 4 at 96,579,442 bp
  • T to C, chromosome 5 at 8,403,184 bp
  • T to C, chromosome 5 at 87,675,740 bp
  • A to G, chromosome 5 at 90,264,064 bp
  • T to A, chromosome 5 at 136,485,847 bp
  • A to G, chromosome 6 at 23,247,528 bp
  • A to G, chromosome 6 at 140,577,290 bp
  • A to G, chromosome 7 at 105,685,692 bp
  • G to A, chromosome 7 at 127,867,769 bp
  • A to G, chromosome 8 at 35,589,118 bp
  • A to G, chromosome 10 at 33,949,435 bp
  • A to G, chromosome 10 at 53,343,696 bp
  • G to T, chromosome 12 at 56,611,938 bp
  • T to A, chromosome 12 at 81,581,875 bp
  • T to C, chromosome 12 at 101,999,593 bp
  • T to A, chromosome 12 at 111,575,083 bp
  • A to T, chromosome 13 at 13,664,116 bp
  • A to G, chromosome 13 at 38,954,136 bp
  • A to G, chromosome 13 at 72,629,129 bp
  • T to C, chromosome 13 at 74,361,361 bp
  • T to C, chromosome 13 at 99,430,022 bp
  • A to T, chromosome 13 at 114,974,105 bp
  • A to G, chromosome 15 at 4,947,935 bp
  • T to C, chromosome 15 at 71,820,059 bp
  • A to G, chromosome 16 at 20,703,245 bp
  • AT to ATGT, chromosome 16 at 22,834,028 bp
  • T to C, chromosome 17 at 14,959,512 bp
  • A to G, chromosome 17 at 33,881,456 bp
Phenotype
G1 male heterozygous mice are viable and fertile. The G1 mice were not characterized prior to archiving.
Strain Development
C57BL/6J mice were injected with ENU, resulting in G0 mice that were bred back to C57BL/6J. Sperm was archived from G1 male mice that produced viable G2 mice.
Suggested Control Mice
Wild-type littermates or C57BL/6J mice
MMRRC Genetic QC Summary
The MMRRC Centers have developed a genetic QC pipeline using MiniMUGA array genotyping to provide additional information on strain backgrounds for MMRRC congenic and inbred strains. For more information on when data may be available, or to request genotyping for a strain of interest, please contact mmrrc@missouri.edu. Older strains may not have this information.
Donor
Bruce Beutler, M.D., University of Texas Southwestern Medical Center.
Primary Reference
MUTAGENETIX record for R6663 (G1). B. Beutler and colleagues, Center for the Genetics of Host Defense, UT Southwestern, Dallas, TX.

Colony and Husbandry Information

Colony Surveillance Program and Current Health Reports

Mice recovered from a cryo-archive will have health surveillance performed on recipient females. Health reports will be provided prior to shipment. If you require additional health status information, please email mmrrc@missouri.edu.
Coat Color
Black
MMRRC Breeding System
Random intra-strain mating
Breeding Scheme(s)
When maintaining a live colony, heterozygous mice may be bred together, bred with wild-type siblings, or bred with C57BL/6J inbred mice.
Overall Breeding Performance
Undetermined; during development, mating was done with heterozygous males; viability, fertility and other breeding statistics for heterozygous females and homozygotes are unverified, but presumably would be similar to the C57BL/6J background strain
Average litter size
Undetermined
Recommended wean age
3 weeks

Order Request Information

Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.

Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.

The donor or their institution limits the distribution to non-profit institutions only.

Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.

Click button to Request this one strain. (Use the MMRRC Catalog Search to request more than one strain.)
MMRRC Item # Description Distribution Fee / Unit (US $)
*Shipping & Handling not included*
Units Notes
044783-MU-RESUS Litter recovered from cryo-archive $5,475.00 / Non-Profit Litter Recovered litter4; additional fees for any special requests.
Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

1 The distribution fee covers the expense of rederiving mice from a live mouse; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

2 An aliquot contains a sufficient number of embryos (in one or more vials or straws and based on the transfer success rate of the MMRRC facility) to transfer into one to three recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.

3 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.

4 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.