Strain Name:
C57BL/6J-MtgxR6677Btlr/Mmmh
Stock Number:
044796-MU
Citation ID:
RRID:MMRRC_044796-MU
Other Names:
R6677 (G1)
Major Collection:

Strain Information

Scn8a
Name: sodium channel, voltage-gated, type VIII, alpha
Synonyms: med, seal, motor end-plate disease, nmf2, ataxia 3, mnd2, mnd-2, C630029C19Rik, nmf58, NaCh6, Nav1.6, nmf335, NMF335, nur14
Type: Gene
Species: Mouse
Chromosome: 15
NCBI: 20273
Homologene: 7927
Akap9
Name: A kinase anchor protein 9
Synonyms: AKAP450, 5730481H23Rik, G1-448-15, repro12, mei2-5
Type: Gene
Species: Mouse
Chromosome: 5
NCBI: 100986
HGNC: HGNC:379
Homologene: 134583
Itprid2
Name: ITPR interacting domain containing 2
Synonyms: SPAG13, CS-1, CS1, KRAP, Ssfa2
Type: Gene
Species: Mouse
Chromosome: 2
NCBI: 70599
Homologene: 4912
Syne1
Name: spectrin repeat containing, nuclear envelope 1
Synonyms: nesprin-1, SYNE-1, enaptin165, A330049M09Rik, C130039F11Rik
Type: Gene
Species: Mouse
Chromosome: 10
NCBI: 64009
VEGA: 10
Homologene: 52329
Polr1b
Name: polymerase (RNA) I polypeptide B
Synonyms: RPA2, RPA116, 128kDa, D630020H17Rik, Rpo1-2
Type: Gene
Species: Mouse
Chromosome: 2
NCBI: 20017
Homologene: 7133
Rnd2
Name: Rho family GTPase 2
Synonyms: Arhn, Rohn
Type: Gene
Species: Mouse
Chromosome: 11
NCBI: 11858
Homologene: 21123
Enpp3
Name: ectonucleotide pyrophosphatase/phosphodiesterase 3
Synonyms: CD203c
Type: Gene
Species: Mouse
Chromosome: 10
NCBI: 209558
VEGA: 10
HGNC: HGNC:3358
Homologene: 3683
Mpp3
Name: membrane protein, palmitoylated 3 (MAGUK p55 subfamily member 3)
Synonyms: Dlgh3
Type: Gene
Species: Mouse
Chromosome: 11
NCBI: 13384
HGNC: HGNC:7221
Homologene: 31065
Lama1
Name: laminin, alpha 1
Synonyms: Lama
Type: Gene
Species: Mouse
Chromosome: 17
NCBI: 16772
VEGA: 17
HGNC: HGNC:6481
Homologene: 21146
Zic5
Name: zinc finger protein of the cerebellum 5
Synonyms: odd-paired related, Opr, 1700049L20Rik
Type: Gene
Species: Mouse
Chromosome: 14
NCBI: 65100
Homologene: 11301
Atl1
Name: atlastin GTPase 1
Synonyms: AD-FSP, SPG3, FSP1, Spg3a
Type: Gene
Species: Mouse
Chromosome: 12
NCBI: 73991
VEGA: 12
Homologene: 9302
Qrfprl
Name: pyroglutamylated RFamide peptide receptor like
Synonyms: C130060K24Rik
Type: Gene
Species: Mouse
Chromosome: 6
NCBI: 243407
Homologene: 135976
Blzf1
Name: basic leucine zipper nuclear factor 1
Synonyms: Blzf1l, Jem-1, 1700030G05Rik, Golgin-45
Type: Gene
Species: Mouse
Chromosome: 1
NCBI: 66352
HGNC: HGNC:1065
Homologene: 31187
Atp12a
Name: ATPase, H+/K+ transporting, nongastric, alpha polypeptide
Synonyms: HKalpha2, cHKA, Atp1al1, ATPase H+K+-transporting, alpha 2
Type: Gene
Species: Mouse
Chromosome: 14
NCBI: 192113
VEGA: 14
Homologene: 68197
Fbxo30
Name: F-box protein 30
Synonyms: 1700026A16Rik
Type: Gene
Species: Mouse
Chromosome: 10
NCBI: 71865
Homologene: 12959
Abcc12
Name: ATP-binding cassette, sub-family C member 12
Synonyms: MRP9, 4930467B22Rik
Type: Gene
Species: Mouse
Chromosome: 8
NCBI: 244562
Homologene: 57211
Ccdc168
Name: coiled-coil domain containing 168
Synonyms: Gm8251
Type: Gene
Species: Mouse
Chromosome: 1
NCBI: 102636082
VEGA: 1
Homologene: 141149
Nhsl1
Name: NHS like 1
Synonyms: A630035H13Rik, D10Bwg0940e, 5730409E15Rik
Type: Gene
Species: Mouse
Chromosome: 10
NCBI: 215819
Homologene: 27834
Bbox1
Name: gamma-butyrobetaine hydroxylase 1
Type: Gene
Species: Mouse
Chromosome: 2
NCBI: 170442
HGNC: HGNC:964
Homologene: 2967
Cfap65
Name: cilia and flagella associated protein 65
Synonyms: B230363K08Rik, Ccdc108
Type: Gene
Species: Mouse
Chromosome: 1
NCBI: 241116
Homologene: 28093
Shroom1
Name: shroom family member 1
Synonyms: 1300007L22Rik, Shrm1, Apx
Type: Gene
Species: Mouse
Chromosome: 11
NCBI: 71774
Homologene: 12411
Mgat4b
Name: mannoside acetylglucosaminyltransferase 4, isoenzyme B
Type: Gene
Species: Mouse
Chromosome: 11
NCBI: 103534
HGNC: HGNC:7048
Homologene: 8611
Chrm2
Name: cholinergic receptor, muscarinic 2, cardiac
Synonyms: M2, AChR M2, muscarinic acetylcholine receptor 2, Chrm-2
Type: Gene
Species: Mouse
Chromosome: 6
NCBI: 243764
HGNC: HGNC:1951
Homologene: 20190
Clca3b
Name: chloride channel accessory 3B
Synonyms: Clca4
Type: Gene
Species: Mouse
Chromosome: 3
NCBI: 229927
HGNC: HGNC:2017
Homologene: 135610
Gm4841
Name: predicted gene 4841
Type: Gene
Species: Mouse
Chromosome: 18
NCBI: 225594
VEGA: 18
Vmn1r113
Name: vomeronasal 1 receptor 113
Synonyms: Gm5748
Type: Gene
Species: Mouse
Chromosome: 7
NCBI: 436135
Homologene: 104166
Slc24a5
Name: solute carrier family 24, member 5
Synonyms: NCX5, F630045L20Rik, NCKX5, Oca6
Type: Gene
Species: Mouse
Chromosome: 2
NCBI: 317750
Homologene: 18400
Saxo2
Name: stabilizer of axonemal microtubules 2
Synonyms: 1700129I04Rik, Fam154b
Type: Gene
Species: Mouse
Chromosome: 7
NCBI: 330577
Homologene: 25135
Sfxn2
Name: sideroflexin 2
Synonyms: F630107H02Rik
Type: Gene
Species: Mouse
Chromosome: 19
NCBI: 94279
VEGA: 19
Homologene: 14227
Sult5a1
Name: sulfotransferase family 5A, member 1
Synonyms: Sultx1
Type: Gene
Species: Mouse
Chromosome: 8
NCBI: 57429
Homologene: 129532
Or13a23-ps1
Name: olfactory receptor family 13 subfamily A member 23, pseudogene 1
Synonyms: GA_x6K02T2PBJ9-42687904-42688835, MOR253-11P, MOR253-15_p, Olfr537-ps1
Type: Gene
Species: Mouse
Chromosome: 7
NCBI: 258200
Genetic Alterations
ENU-induced transitions at the following base pair locations (GRCm38):
  • G to T, chromosome 1 at 44,058,699 bp
  • C to A, chromosome 1 at 74,904,685 bp
  • A to T, chromosome 1 at 164,302,612 bp
  • A to G, chromosome 2 at 79,655,101 bp
  • C to A, chromosome 2 at 110,305,425 bp
  • G to T, chromosome 2 at 125,080,695 bp
  • T to C, chromosome 2 at 129,120,211 bp
  • T to A, chromosome 3 at 144,823,384 bp
  • A to G, chromosome 5 at 4,029,869 bp
  • A to T, chromosome 6 at 36,524,092 bp
  • A to T, chromosome 6 at 65,456,245 bp
  • G to A, chromosome 7 at 20,787,978 bp
  • T to C, chromosome 7 at 82,635,276 bp
  • T to G, chromosome 7 at 140,538,552 bp
  • G to T, chromosome 8 at 86,534,752 bp
  • A to G, chromosome 8 at 123,149,278 bp
  • T to C, chromosome 10 at 5,040,942 bp
  • G to T, chromosome 10 at 11,290,636 bp
  • T to A, chromosome 10 at 18,525,862 bp
  • T to A, chromosome 10 at 24,777,957 bp
  • A to G, chromosome 11 at 50,233,071 bp
  • G to A, chromosome 11 at 53,463,516 bp
  • C to T, chromosome 11 at 101,468,999 bp
  • A to G, chromosome 11 at 102,008,618 bp
  • A to G, chromosome 12 at 69,953,444 bp
  • A to G, chromosome 14 at 56,380,854 bp
  • C to G, chromosome 14 at 122,465,154 bp
  • A to G, chromosome 15 at 100,969,072 bp
  • G to A, chromosome 17 at 67,795,233 bp
  • A to T, chromosome 18 at 60,270,580 bp
  • A to T, chromosome 19 at 46,582,528 bp
Phenotype
G1 male heterozygous mice are viable and fertile. The G1 mice were not characterized prior to archiving.
Strain Development
C57BL/6J mice were injected with ENU, resulting in G0 mice that were bred back to C57BL/6J. Sperm was archived from G1 male mice that produced viable G2 mice.
Suggested Control Mice
Wild-type littermates or C57BL/6J mice
MMRRC Genetic QC Summary
The MMRRC Centers have developed a genetic QC pipeline using MiniMUGA array genotyping to provide additional information on strain backgrounds for MMRRC congenic and inbred strains. For more information on when data may be available, or to request genotyping for a strain of interest, please contact mmrrc@missouri.edu. Older strains may not have this information.
Donor
Bruce Beutler, M.D., University of Texas Southwestern Medical Center.
Primary Reference
MUTAGENETIX record for R6677 (G1). B. Beutler and colleagues, Center for the Genetics of Host Defense, UT Southwestern, Dallas, TX.

Colony and Husbandry Information

Colony Surveillance Program and Current Health Reports

Mice recovered from a cryo-archive will have health surveillance performed on recipient females. Health reports will be provided prior to shipment. If you require additional health status information, please email mmrrc@missouri.edu.
Coat Color
Black
MMRRC Breeding System
Random intra-strain mating
Breeding Scheme(s)
When maintaining a live colony, heterozygous mice may be bred together, bred with wild-type siblings, or bred with C57BL/6J inbred mice.
Overall Breeding Performance
Undetermined; during development, mating was done with heterozygous males; viability, fertility and other breeding statistics for heterozygous females and homozygotes are unverified, but presumably would be similar to the C57BL/6J background strain
Average litter size
Undetermined
Recommended wean age
3 weeks

Order Request Information

Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.

Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.

The donor or their institution limits the distribution to non-profit institutions only.

Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.

Click button to Request this one strain. (Use the MMRRC Catalog Search to request more than one strain.)
MMRRC Item # Description Distribution Fee / Unit (US $)
*Shipping & Handling not included*
Units Notes
044796-MU-RESUS Litter recovered from cryo-archive $5,475.00 / Non-Profit Litter Recovered litter4; additional fees for any special requests.
Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

1 The distribution fee covers the expense of rederiving mice from a live mouse; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

2 An aliquot contains a sufficient number of embryos (in one or more vials or straws and based on the transfer success rate of the MMRRC facility) to transfer into one to three recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.

3 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.

4 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.