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Strain Name:
C57BL/6J-MtgxR6722Btlr/Mmmh
Stock Number:
044840-MU
Citation ID:
RRID:MMRRC_044840-MU
Other Names:
R6722 (G1)
Major Collection:

Strain Information

Pdx1
Name: pancreatic and duodenal homeobox 1
Synonyms: IDX-1, STF-1, Ipf1, IPF-1, Mody4, pdx-1
Type: Gene
Species: Mouse
Chromosome: 5
NCBI: 18609
HGNC: HGNC:6107
Homologene: 175
Sez6
Name: seizure related gene 6
Synonyms: sez-6, D11Bhm177e
Type: Gene
Species: Mouse
Chromosome: 11
NCBI: 20370
Homologene: 10948
Pzp2
Name: PZP alpha-2-macroglobulin like 2
Synonyms: Pzp
Type: Gene
Species: Mouse
Chromosome: 6
NCBI: 11287
Homologene: 104112
Rbpms
Name: RNA binding protein gene with multiple splicing
Synonyms: hermes, 2700019M19Rik, 2010300K22Rik
Type: Gene
Species: Mouse
Chromosome: 8
NCBI: 19663
Homologene: 38238
Mbd2
Name: methyl-CpG binding domain protein 2
Synonyms: MBD2a
Type: Gene
Species: Mouse
Chromosome: 18
NCBI: 17191
HGNC: HGNC:6917
Homologene: 2918
Triobp
Name: TRIO and F-actin binding protein
Synonyms: Mus EST 478828, Tara, EST478828
Type: Gene
Species: Mouse
Chromosome: 15
NCBI: 110253
Homologene: 5104
Parvb
Name: parvin, beta
Synonyms: D15Gsk1, affixin
Type: Gene
Species: Mouse
Chromosome: 15
NCBI: 170736
Homologene: 8342
Genetic Alterations
ENU-induced transitions at the following base pair locations (GRCm38):
  • T to A, chromosome 1 at 45,805,352 bp
  • T to C, chromosome 1 at 74,681,789 bp
  • G to A, chromosome 1 at 100,478,486 bp
  • G to A, chromosome 1 at 171,872,777 bp
  • T to C, chromosome 1 at 172,258,050 bp
  • A to T, chromosome 2 at 29,788,285 bp
  • T to A, chromosome 2 at 112,156,882 bp
  • T to A, chromosome 2 at 164,820,624 bp
  • C to T, chromosome 3 at 88,353,025 bp
  • G to A, chromosome 3 at 97,718,239 bp
  • A to G, chromosome 3 at 108,816,446 bp
  • A to G, chromosome 4 at 3,973,386 bp
  • T to A, chromosome 4 at 21,859,165 bp
  • A to G, chromosome 4 at 43,547,618 bp
  • A to C, chromosome 4 at 118,584,717 bp
  • T to C, chromosome 4 at 147,583,071 bp
  • T to G, chromosome 4 at 152,160,377 bp
  • T to A, chromosome 5 at 104,520,279 bp
  • T to C, chromosome 5 at 134,135,619 bp
  • C to T, chromosome 5 at 147,270,500 bp
  • T to C, chromosome 6 at 39,805,665 bp
  • T to A, chromosome 6 at 128,487,954 bp
  • T to A, chromosome 6 at 146,818,900 bp
  • T to A, chromosome 7 at 5,610,688 bp
  • A to G, chromosome 7 at 26,313,558 bp
  • G to T, chromosome 8 at 33,834,393 bp
  • G to A, chromosome 8 at 46,020,563 bp
  • T to C, chromosome 8 at 105,944,250 bp
  • G to A, chromosome 9 at 21,211,225 bp
  • T to C, chromosome 9 at 27,087,556 bp
  • A to G, chromosome 9 at 54,699,754 bp
  • T to C, chromosome 9 at 110,632,992 bp
  • T to C, chromosome 10 at 41,644,641 bp
  • C to T, chromosome 10 at 42,860,732 bp
  • T to C, chromosome 10 at 69,990,244 bp
  • C to T, chromosome 10 at 76,614,558 bp
  • G to A, chromosome 11 at 23,702,975 bp
  • A to C, chromosome 11 at 74,865,424 bp
  • T to C, chromosome 11 at 77,953,702 bp
  • A to G, chromosome 11 at 100,048,428 bp
  • A to G, chromosome 11 at 102,399,949 bp
  • A to G, chromosome 11 at 106,382,481 bp
  • T to A, chromosome 12 at 10,372,874 bp
  • T to G, chromosome 12 at 81,421,454 bp
  • C to A, chromosome 13 at 109,632,898 bp
  • T to C, chromosome 14 at 30,569,084 bp
  • G to T, chromosome 14 at 64,095,059 bp
  • G to A, chromosome 15 at 79,001,565 bp
  • C to T, chromosome 15 at 84,297,979 bp
  • C to A, chromosome 15 at 85,905,914 bp
  • A to G, chromosome 17 at 24,634,034 bp
  • T to C, chromosome 17 at 57,066,802 bp
  • T to A, chromosome 18 at 70,580,748 bp
  • A to T, chromosome 19 at 34,121,265 bp
Phenotype
G1 male heterozygous mice are viable and fertile. The G1 mice were not characterized prior to archiving.
Strain GQC Summary
Gene Specific Genotyping:

To request gene-specific and other genotyping services for a strain, please contact the distribution MMRRC Center for more information.

Background Genetic Quality:

The MMRRC has developed a Genetic Quality Control pipeline using the MiniMUGA array to provide additional information to identify and validate genetic backgrounds of MMRRC strains. For more information on whether genetic background data is available, please contact MMRRC_GeneticQC@med.unc.edu. Note: that MiniMUGA genetic background data is not available on all strains, but can be ordered if desired.

Suggested Control Mice
Littermates of all relevant genotypes.
Donor
Bruce Beutler, M.D., University of Texas Southwestern Medical Center.
Primary Reference
MUTAGENETIX record for R6722 (G1). B. Beutler and colleagues, Center for the Genetics of Host Defense, UT Southwestern, Dallas, TX.
Strain Development
C57BL/6J mice were injected with ENU, resulting in G0 mice that were bred back to C57BL/6J. Sperm was archived from G1 male mice that produced viable G2 mice.


Disclaimer: If MMRRC Strain Genetic Quality Control (GQC; based on MiniMUGA genotyping and analysis) has been completed for this strain, the information might differ from the genetic background information provided by the submitter. MiniMUGA genetic analysis is done on a strain’s tissue samples taken when archived by or ordered from the assigned MMRRC Center.

Colony and Husbandry Information

Colony Surveillance Program and Current Health Reports

Mice recovered from a cryo-archive will have health surveillance performed on recipient females. Health reports will be provided prior to shipment. If you require additional health status information, please email mmrrc@missouri.edu.
Coat Color
Black
MMRRC Breeding System
Random intra-strain mating
Breeding Scheme(s)
When maintaining a live colony, heterozygous mice may be bred together, bred with wild-type siblings, or bred with C57BL/6J inbred mice.
Overall Breeding Performance
Undetermined; during development, mating was done with heterozygous males; viability, fertility and other breeding statistics for heterozygous females and homozygotes are unverified, but presumably would be similar to the C57BL/6J background strain
Average litter size
Undetermined
Recommended wean age
3 weeks

Order Information

Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.

Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.

The donor or their institution limits the distribution to non-profit institutions only.

Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.

Click button to Request this one strain. (Use the MMRRC Catalog Search to request more than one strain.)
MMRRC Item # Description Distribution Fee / Unit (US $)
*Shipping & Handling not included*
Units Notes
044840-MU-RESUS Litter recovered from cryo-archive $5,475.00 / Non-Profit Litter Recovered litter4; additional fees for any special requests.
Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

1 The distribution fee covers the expense of rederiving mice from a live mouse; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

2 An aliquot contains a sufficient number of embryos (in one or more vials or straws and based on the transfer success rate of the MMRRC facility) to transfer into one to three recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.

3 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.

4 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.