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Strain Name:
C57BL/6J-MtgxR6748Btlr/Mmmh
Stock Number:
044865-MU
Citation ID:
RRID:MMRRC_044865-MU
Other Names:
R6748 (G1)
Major Collection:

Strain Information

Drd2
Name: dopamine receptor D2
Synonyms: D2 receptor, D2R, Drd-2
Type: Gene
Species: Mouse
Chromosome: 9
NCBI: 13489
VEGA: 9
HGNC: HGNC:3023
Homologene: 22561
Mtor
Name: mechanistic target of rapamycin kinase
Synonyms: FKBP-rapamycin-associated protein FRAP, 2610315D21Rik, RAPT1, RAFT1, flat, Frap1, mechanistic target of rapamycin (serine/threonine kinase)
Type: Gene
Species: Mouse
Chromosome: 4
NCBI: 56717
HGNC: HGNC:3942
Homologene: 3637
Mat2b
Name: methionine adenosyltransferase 2 non-catalytic beta subunit methionine
Synonyms: MATIIbeta, 2410018D16Rik, 1110064C04Rik
Type: Gene
Species: Mouse
Chromosome: 11
NCBI: 108645
HGNC: HGNC:6905
Homologene: 8323
Cdc42bpb
Name: CDC42 binding protein kinase beta
Synonyms: DMPK-like
Type: Gene
Species: Mouse
Chromosome: 12
NCBI: 217866
VEGA: 12
HGNC: HGNC:1738
Homologene: 55945
Ttc17
Name: tetratricopeptide repeat domain 17
Synonyms: D2Bwg1005e, 9130020K17Rik
Type: Gene
Species: Mouse
Chromosome: 2
NCBI: 74569
Homologene: 10100
Zfp407
Name: zinc finger protein 407
Synonyms: LOC240469, LOC381139, 6430585N13Rik
Type: Gene
Species: Mouse
Chromosome: 18
NCBI: 240476
Homologene: 86402
Dock4
Name: dedicator of cytokinesis 4
Synonyms: EST N28122, 6330411N01Rik
Type: Gene
Species: Mouse
Chromosome: 12
NCBI: 238130
VEGA: 12
Homologene: 56680
Genetic Alterations
ENU-induced transitions at the following base pair locations (GRCm38):
  • A to G, chromosome 1 at 24,534,070 bp
  • C to A, chromosome 1 at 38,535,246 bp
  • A to G, chromosome 1 at 64,735,564 bp
  • A to G, chromosome 1 at 171,404,810 bp
  • A to G, chromosome 1 at 194,794,182 bp
  • T to C, chromosome 2 at 76,746,084 bp
  • T to C, chromosome 2 at 94,386,102 bp
  • T to A, chromosome 2 at 111,912,357 bp
  • A to G, chromosome 2 at 144,566,794 bp
  • T to A, chromosome 3 at 109,149,507 bp
  • T to A, chromosome 4 at 45,274,397 bp
  • T to A, chromosome 4 at 96,475,545 bp
  • T to C, chromosome 4 at 139,669,611 bp
  • T to C, chromosome 4 at 148,550,184 bp
  • A to G, chromosome 5 at 36,495,254 bp
  • T to C, chromosome 5 at 89,435,572 bp
  • T to C, chromosome 5 at 93,852,213 bp
  • C to T, chromosome 6 at 48,906,294 bp
  • T to C, chromosome 6 at 122,933,897 bp
  • T to G, chromosome 7 at 105,720,637 bp
  • C to T, chromosome 8 at 125,850,335 bp
  • A to T, chromosome 8 at 126,394,638 bp
  • T to A, chromosome 9 at 49,403,202 bp
  • A to G, chromosome 9 at 65,310,159 bp
  • TCCC to TCC, chromosome 9 at 66,501,188 bp
  • A to G, chromosome 9 at 66,833,295 bp
  • A to G, chromosome 10 at 79,762,136 bp
  • A to T, chromosome 10 at 80,525,224 bp
  • A to G, chromosome 11 at 32,300,169 bp
  • T to A, chromosome 11 at 40,680,194 bp
  • T to A, chromosome 11 at 48,916,296 bp
  • G to A, chromosome 11 at 50,922,832 bp
  • A to G, chromosome 12 at 35,947,595 bp
  • A to T, chromosome 12 at 40,704,466 bp
  • T to C, chromosome 12 at 111,294,839 bp
  • C to A, chromosome 13 at 23,232,947 bp
  • T to A, chromosome 13 at 67,182,624 bp
  • A to T, chromosome 13 at 104,208,030 bp
  • A to G, chromosome 14 at 31,299,988 bp
  • A to G, chromosome 14 at 60,779,827 bp
  • A to G, chromosome 14 at 69,175,106 bp
  • A to T, chromosome 16 at 26,722,356 bp
  • T to A, chromosome 16 at 33,899,297 bp
  • T to A, chromosome 16 at 59,085,890 bp
  • A to C, chromosome 18 at 37,518,746 bp
  • G to A, chromosome 18 at 74,701,503 bp
  • G to A, chromosome 18 at 84,208,830 bp
Phenotype
G1 male heterozygous mice are viable and fertile. The G1 mice were not characterized prior to archiving.
Strain GQC Summary
Gene Specific Genotyping:

To request gene-specific and other genotyping services for a strain, please contact the distribution MMRRC Center for more information.

Background Genetic Quality:

The MMRRC has developed a Genetic Quality Control pipeline using the MiniMUGA array to provide additional information to identify and validate genetic backgrounds of MMRRC strains. For more information on whether genetic background data is available, please contact MMRRC_GeneticQC@med.unc.edu. Note: that MiniMUGA genetic background data is not available on all strains, but can be ordered if desired.

Suggested Control Mice
Littermates of all relevant genotypes.
Donor
Bruce Beutler, M.D., University of Texas Southwestern Medical Center.
Primary Reference
MUTAGENETIX record for R6748 (G1). B. Beutler and colleagues, Center for the Genetics of Host Defense, UT Southwestern, Dallas, TX.
Strain Development
C57BL/6J mice were injected with ENU, resulting in G0 mice that were bred back to C57BL/6J. Sperm was archived from G1 male mice that produced viable G2 mice.


Disclaimer: If MMRRC Strain Genetic Quality Control (GQC; based on MiniMUGA genotyping and analysis) has been completed for this strain, the information might differ from the genetic background information provided by the submitter. MiniMUGA genetic analysis is done on a strain’s tissue samples taken when archived by or ordered from the assigned MMRRC Center.

Colony and Husbandry Information

Colony Surveillance Program and Current Health Reports

Mice recovered from a cryo-archive will have health surveillance performed on recipient females. Health reports will be provided prior to shipment. If you require additional health status information, please email mmrrc@missouri.edu.
Coat Color
Black
MMRRC Breeding System
Random intra-strain mating
Breeding Scheme(s)
When maintaining a live colony, heterozygous mice may be bred together, bred with wild-type siblings, or bred with C57BL/6J inbred mice.
Overall Breeding Performance
Undetermined; during development, mating was done with heterozygous males; viability, fertility and other breeding statistics for heterozygous females and homozygotes are unverified, but presumably would be similar to the C57BL/6J background strain
Average litter size
Undetermined
Recommended wean age
3 weeks

Order Information

Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.

Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.

The donor or their institution limits the distribution to non-profit institutions only.

Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.

Click button to Request this one strain. (Use the MMRRC Catalog Search to request more than one strain.)
MMRRC Item # Description Distribution Fee / Unit (US $)
*Shipping & Handling not included*
Units Notes
044865-MU-RESUS Litter recovered from cryo-archive $5,475.00 / Non-Profit Litter Recovered litter4; additional fees for any special requests.
Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

1 The distribution fee covers the expense of rederiving mice from a live mouse; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

2 An aliquot contains a sufficient number of embryos (in one or more vials or straws and based on the transfer success rate of the MMRRC facility) to transfer into one to three recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.

3 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.

4 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.