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Strain Name:
C57BL/6J-MtgxR6750Btlr/Mmmh
Stock Number:
044867-MU
Citation ID:
RRID:MMRRC_044867-MU
Other Names:
R6750 (G1)
Major Collection:

Strain Information

Nrg1
Name: neuregulin 1
Synonyms: HGL, HRG, Hgl, SMDF, GGF, ARIA, heregulin, D230005F13Rik, HRGalpha, 6030402G23Rik, GGFII, NDF
Type: Gene
Species: Mouse
Chromosome: 8
NCBI: 211323
HGNC: HGNC:7997
Homologene: 138451
Ptprf
Name: protein tyrosine phosphatase receptor type F
Synonyms: LAR, RPTP-LAR
Type: Gene
Species: Mouse
Chromosome: 4
NCBI: 19268
HGNC: HGNC:9670
Homologene: 20623
Tmem59l
Name: transmembrane protein 59-like
Synonyms: 5330410G16Rik
Type: Gene
Species: Mouse
Chromosome: 8
NCBI: 67937
Homologene: 8104
Fryl
Name: FRY like transcription coactivator
Synonyms: 2310004H21Rik, 2510002A14Rik, 9030227G01Rik
Type: Gene
Species: Mouse
Chromosome: 5
NCBI: 72313
Homologene: 103956
Psme4
Name: proteasome (prosome, macropain) activator subunit 4
Type: Gene
Species: Mouse
Chromosome: 11
NCBI: 103554
Homologene: 113742
Sec16a
Name: SEC16 homolog A, endoplasmic reticulum export factor
Synonyms: C230052J16Rik
Type: Gene
Species: Mouse
Chromosome: 2
NCBI: 227648
Homologene: 10533
Akap13
Name: A kinase anchor protein 13
Synonyms: AKAP-Lbc, PROTO-LBC, PROTO-LB, Ht31, 5730522G15Rik, 1700026G02Rik, 5830460E08Rik
Type: Gene
Species: Mouse
Chromosome: 7
NCBI: 75547
HGNC: HGNC:371
Homologene: 4903
Genetic Alterations
ENU-induced transitions at the following base pair locations (GRCm38):
  • C to A, chromosome 1 at 28,777,414 bp
  • A to G, chromosome 1 at 45,817,379 bp
  • G to C, chromosome 1 at 93,826,407 bp
  • C to T, chromosome 1 at 100,274,499 bp
  • A to G, chromosome 1 at 164,193,507 bp
  • A to G, chromosome 2 at 26,440,018 bp
  • T to C, chromosome 2 at 27,228,257 bp
  • C to A, chromosome 2 at 36,812,942 bp
  • A to T, chromosome 2 at 121,142,649 bp
  • A to G, chromosome 3 at 36,624,393 bp
  • A to T, chromosome 4 at 72,122,450 bp
  • A to G, chromosome 4 at 118,231,731 bp
  • A to C, chromosome 4 at 128,197,225 bp
  • T to A, chromosome 5 at 12,585,100 bp
  • T to C, chromosome 5 at 31,189,960 bp
  • T to C, chromosome 5 at 37,265,322 bp
  • A to G, chromosome 5 at 73,022,232 bp
  • C to T, chromosome 5 at 121,408,309 bp
  • T to A, chromosome 5 at 129,696,117 bp
  • A to G, chromosome 5 at 136,100,948 bp
  • T to C, chromosome 6 at 115,549,920 bp
  • G to T, chromosome 6 at 126,898,955 bp
  • A to G, chromosome 6 at 132,802,854 bp
  • T to C, chromosome 6 at 140,028,438 bp
  • T to A, chromosome 7 at 20,023,234 bp
  • T to C, chromosome 7 at 56,097,447 bp
  • C to T, chromosome 7 at 75,739,458 bp
  • T to C, chromosome 7 at 86,225,906 bp
  • C to T, chromosome 7 at 104,618,113 bp
  • C to T, chromosome 7 at 112,381,839 bp
  • T to A, chromosome 7 at 131,288,245 bp
  • A to C, chromosome 8 at 31,818,096 bp
  • A to G, chromosome 8 at 60,914,775 bp
  • G to A, chromosome 8 at 70,486,372 bp
  • T to A, chromosome 8 at 123,073,911 bp
  • G to A, chromosome 9 at 19,647,804 bp
  • A to G, chromosome 9 at 44,750,394 bp
  • T to C, chromosome 9 at 49,567,339 bp
  • A to T, chromosome 9 at 57,700,256 bp
  • A to G, chromosome 9 at 66,220,752 bp
  • TCCC to TCC, chromosome 9 at 66,501,188 bp
  • G to A, chromosome 9 at 73,085,008 bp
  • T to A, chromosome 9 at 89,699,790 bp
  • A to T, chromosome 9 at 95,560,997 bp
  • A to G, chromosome 9 at 105,783,680 bp
  • T to A, chromosome 10 at 19,609,351 bp
  • A to T, chromosome 10 at 41,561,226 bp
  • T to C, chromosome 10 at 43,582,725 bp
  • T to C, chromosome 10 at 81,514,283 bp
  • A to T, chromosome 11 at 8,973,217 bp
  • A to T, chromosome 11 at 30,853,203 bp
  • A to T, chromosome 11 at 59,696,131 bp
  • A to T, chromosome 11 at 61,157,216 bp
  • C to T, chromosome 11 at 99,348,980 bp
  • T to A, chromosome 12 at 7,997,853 bp
  • T to A, chromosome 12 at 16,688,583 bp
  • T to C, chromosome 12 at 31,332,214 bp
  • C to T, chromosome 12 at 57,542,610 bp
  • C to A, chromosome 12 at 76,775,631 bp
  • T to A, chromosome 12 at 85,956,610 bp
  • G to C, chromosome 13 at 21,981,937 bp
  • A to G, chromosome 13 at 24,464,252 bp
  • T to A, chromosome 13 at 32,802,756 bp
  • A to T, chromosome 13 at 65,181,654 bp
  • T to G, chromosome 13 at 114,826,248 bp
  • C to T, chromosome 15 at 76,538,412 bp
  • C to T, chromosome 16 at 88,506,307 bp
  • T to A, chromosome 17 at 23,607,190 bp
  • A to T, chromosome 17 at 33,066,398 bp
  • T to C, chromosome 18 at 37,514,448 bp
  • A to T, chromosome 18 at 74,617,035 bp
  • A to G, chromosome 19 at 23,242,640 bp
  • C to T, chromosome 19 at 31,764,561 bp
  • T to G, chromosome 19 at 45,027,748 bp
Phenotype
G1 male heterozygous mice are viable and fertile. The G1 mice were not characterized prior to archiving.
Strain GQC Summary
Gene Specific Genotyping:

To request gene-specific and other genotyping services for a strain, please contact the distribution MMRRC Center for more information.

Background Genetic Quality:

The MMRRC has developed a Genetic Quality Control pipeline using the MiniMUGA array to provide additional information to identify and validate genetic backgrounds of MMRRC strains. For more information on whether genetic background data is available, please contact MMRRC_GeneticQC@med.unc.edu. Note: that MiniMUGA genetic background data is not available on all strains, but can be ordered if desired.

Suggested Control Mice
Littermates of all relevant genotypes.
Donor
Bruce Beutler, M.D., University of Texas Southwestern Medical Center.
Primary Reference
MUTAGENETIX record for R6750 (G1). B. Beutler and colleagues, Center for the Genetics of Host Defense, UT Southwestern, Dallas, TX.
Strain Development
C57BL/6J mice were injected with ENU, resulting in G0 mice that were bred back to C57BL/6J. Sperm was archived from G1 male mice that produced viable G2 mice.


Disclaimer: If MMRRC Strain Genetic Quality Control (GQC; based on MiniMUGA genotyping and analysis) has been completed for this strain, the information might differ from the genetic background information provided by the submitter. MiniMUGA genetic analysis is done on a strain’s tissue samples taken when archived by or ordered from the assigned MMRRC Center.

Colony and Husbandry Information

Colony Surveillance Program and Current Health Reports

Mice recovered from a cryo-archive will have health surveillance performed on recipient females. Health reports will be provided prior to shipment. If you require additional health status information, please email mmrrc@missouri.edu.
Coat Color
Black
MMRRC Breeding System
Random intra-strain mating
Breeding Scheme(s)
When maintaining a live colony, heterozygous mice may be bred together, bred with wild-type siblings, or bred with C57BL/6J inbred mice.
Overall Breeding Performance
Undetermined; during development, mating was done with heterozygous males; viability, fertility and other breeding statistics for heterozygous females and homozygotes are unverified, but presumably would be similar to the C57BL/6J background strain
Average litter size
Undetermined
Recommended wean age
3 weeks

Order Information

Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.

Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.

The donor or their institution limits the distribution to non-profit institutions only.

Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.

Click button to Request this one strain. (Use the MMRRC Catalog Search to request more than one strain.)
MMRRC Item # Description Distribution Fee / Unit (US $)
*Shipping & Handling not included*
Units Notes
044867-MU-RESUS Litter recovered from cryo-archive $5,475.00 / Non-Profit Litter Recovered litter4; additional fees for any special requests.
Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

1 The distribution fee covers the expense of rederiving mice from a live mouse; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

2 An aliquot contains a sufficient number of embryos (in one or more vials or straws and based on the transfer success rate of the MMRRC facility) to transfer into one to three recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.

3 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.

4 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.