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Strain Name:
C57BL/6J-MtgxR6751Btlr/Mmmh
Stock Number:
044868-MU
Citation ID:
RRID:MMRRC_044868-MU
Other Names:
R6751 (G1)
Major Collection:

Strain Information

Chd7
Name: chromodomain helicase DNA binding protein 7
Synonyms: GENA 60, GENA 47, Gena 52, Cyn, A730019I05Rik, WBE1, Whi, Todo, Obt, Mt, Lda, Flo, Edy, Dz, Cycn
Type: Gene
Species: Mouse
Chromosome: 4
NCBI: 320790
Homologene: 19067
Sptbn1
Name: spectrin beta, non-erythrocytic 1
Synonyms: beta fodrin, Spnb-2, spectrin G, brain spectrin, elf3, elf1, 9930031C03Rik, non-erythrocytic, Spnb2
Type: Gene
Species: Mouse
Chromosome: 11
NCBI: 20742
Homologene: 2354
Chrnb2
Name: cholinergic receptor nicotinic beta 2 subunit
Synonyms: [b]2-nAchR, Acrb-2, Acrb2, C030030P04Rik
Type: Gene
Species: Mouse
Chromosome: 3
NCBI: 11444
HGNC: HGNC:1962
Homologene: 595
Frem2
Name: Fras1 related extracellular matrix protein 2
Synonyms: 8430406N05Rik, 6030440P17Rik, my, ne, b2b1562Clo
Type: Gene
Species: Mouse
Chromosome: 3
NCBI: 242022
Homologene: 18454
Pik3cb
Name: phosphatidylinositol-4,5-bisphosphate 3-kinase catalytic subunit beta
Synonyms: 1110001J02Rik, p110beta
Type: Gene
Species: Mouse
Chromosome: 9
NCBI: 74769
HGNC: HGNC:8976
Homologene: 21250
Zranb3
Name: zinc finger, RAN-binding domain containing 3
Synonyms: 4933425L19Rik
Type: Gene
Species: Mouse
Chromosome: 1
NCBI: 226409
Homologene: 32770
Asap1
Name: ArfGAP with SH3 domain, ankyrin repeat and PH domain1
Synonyms: Ddef1
Type: Gene
Species: Mouse
Chromosome: 15
NCBI: 13196
HGNC: HGNC:2720
Homologene: 7684
Genetic Alterations
ENU-induced transitions at the following base pair locations (GRCm38):
  • C to A, chromosome 1 at 19,103,283 bp
  • A to G, chromosome 1 at 20,779,489 bp
  • T to C, chromosome 1 at 88,384,706 bp
  • T to A, chromosome 1 at 127,959,819 bp
  • T to A, chromosome 1 at 150,734,518 bp
  • A to T, chromosome 2 at 16,548,141 bp
  • T to A, chromosome 2 at 28,709,945 bp
  • A to G, chromosome 2 at 29,228,043 bp
  • T to C, chromosome 2 at 152,396,758 bp
  • A to T, chromosome 3 at 53,653,665 bp
  • A to T, chromosome 3 at 89,761,576 bp
  • A to T, chromosome 3 at 138,066,210 bp
  • T to C, chromosome 4 at 8,833,866 bp
  • T to A, chromosome 4 at 40,188,330 bp
  • T to C, chromosome 4 at 88,827,750 bp
  • A to T, chromosome 4 at 102,602,671 bp
  • T to A, chromosome 4 at 115,491,194 bp
  • G to T, chromosome 4 at 128,585,875 bp
  • C to A, chromosome 5 at 36,204,959 bp
  • T to A, chromosome 5 at 131,081,590 bp
  • T to A, chromosome 5 at 145,290,563 bp
  • A to G, chromosome 6 at 3,600,274 bp
  • C to T, chromosome 7 at 7,203,717 bp
  • T to C, chromosome 7 at 12,694,792 bp
  • C to T, chromosome 7 at 22,789,881 bp
  • C to A, chromosome 7 at 28,186,709 bp
  • T to C, chromosome 7 at 57,418,334 bp
  • G to A, chromosome 7 at 85,619,887 bp
  • A to T, chromosome 7 at 96,845,712 bp
  • G to GACGGCGGCT, chromosome 7 at 97,579,909 bp
  • G to A, chromosome 7 at 102,578,499 bp
  • T to G, chromosome 9 at 38,115,975 bp
  • T to A, chromosome 9 at 39,819,897 bp
  • T to C, chromosome 9 at 39,873,680 bp
  • G to A, chromosome 9 at 62,768,584 bp
  • TCCC to TCC, chromosome 9 at 66,501,188 bp
  • A to G, chromosome 9 at 72,961,975 bp
  • G to T, chromosome 9 at 99,094,521 bp
  • T to C, chromosome 9 at 119,671,551 bp
  • C to T, chromosome 10 at 23,869,625 bp
  • T to A, chromosome 10 at 68,041,453 bp
  • T to C, chromosome 10 at 80,586,587 bp
  • A to G, chromosome 10 at 82,283,497 bp
  • T to C, chromosome 10 at 111,275,013 bp
  • T to C, chromosome 10 at 111,506,694 bp
  • T to C, chromosome 11 at 30,117,859 bp
  • A to G, chromosome 11 at 78,208,949 bp
  • T to C, chromosome 11 at 78,751,124 bp
  • T to C, chromosome 11 at 83,981,425 bp
  • C to A, chromosome 11 at 107,877,553 bp
  • C to T, chromosome 12 at 40,203,914 bp
  • T to C, chromosome 12 at 98,865,400 bp
  • A to G, chromosome 12 at 104,151,500 bp
  • A to T, chromosome 12 at 116,213,456 bp
  • T to A, chromosome 13 at 40,728,754 bp
  • A to T, chromosome 13 at 65,194,429 bp
  • G to A, chromosome 13 at 98,075,247 bp
  • A to G, chromosome 14 at 115,369,951 bp
  • A to T, chromosome 15 at 36,597,534 bp
  • A to G, chromosome 15 at 64,094,412 bp
  • T to C, chromosome 15 at 98,596,205 bp
  • A to G, chromosome 15 at 99,423,161 bp
  • T to C, chromosome 16 at 36,515,588 bp
  • G to A, chromosome 18 at 75,286,647 bp
Phenotype
G1 male heterozygous mice are viable and fertile. The G1 mice were not characterized prior to archiving.
Strain GQC Summary
Gene Specific Genotyping:

To request gene-specific and other genotyping services for a strain, please contact the distribution MMRRC Center for more information.

Background Genetic Quality:

The MMRRC has developed a Genetic Quality Control pipeline using the MiniMUGA array to provide additional information to identify and validate genetic backgrounds of MMRRC strains. For more information on whether genetic background data is available, please contact MMRRC_GeneticQC@med.unc.edu. Note: that MiniMUGA genetic background data is not available on all strains, but can be ordered if desired.

Suggested Control Mice
Littermates of all relevant genotypes.
Donor
Bruce Beutler, M.D., University of Texas Southwestern Medical Center.
Primary Reference
MUTAGENETIX record for R6751 (G1). B. Beutler and colleagues, Center for the Genetics of Host Defense, UT Southwestern, Dallas, TX.
Strain Development
C57BL/6J mice were injected with ENU, resulting in G0 mice that were bred back to C57BL/6J. Sperm was archived from G1 male mice that produced viable G2 mice.


Disclaimer: If MMRRC Strain Genetic Quality Control (GQC; based on MiniMUGA genotyping and analysis) has been completed for this strain, the information might differ from the genetic background information provided by the submitter. MiniMUGA genetic analysis is done on a strain’s tissue samples taken when archived by or ordered from the assigned MMRRC Center.

Colony and Husbandry Information

Colony Surveillance Program and Current Health Reports

Mice recovered from a cryo-archive will have health surveillance performed on recipient females. Health reports will be provided prior to shipment. If you require additional health status information, please email mmrrc@missouri.edu.
Coat Color
Black
MMRRC Breeding System
Random intra-strain mating
Breeding Scheme(s)
When maintaining a live colony, heterozygous mice may be bred together, bred with wild-type siblings, or bred with C57BL/6J inbred mice.
Overall Breeding Performance
Undetermined; during development, mating was done with heterozygous males; viability, fertility and other breeding statistics for heterozygous females and homozygotes are unverified, but presumably would be similar to the C57BL/6J background strain
Average litter size
Undetermined
Recommended wean age
3 weeks

Order Information

Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.

Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.

The donor or their institution limits the distribution to non-profit institutions only.

Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.

Click button to Request this one strain. (Use the MMRRC Catalog Search to request more than one strain.)
MMRRC Item # Description Distribution Fee / Unit (US $)
*Shipping & Handling not included*
Units Notes
044868-MU-RESUS Litter recovered from cryo-archive $5,475.00 / Non-Profit Litter Recovered litter4; additional fees for any special requests.
Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

1 The distribution fee covers the expense of rederiving mice from a live mouse; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

2 An aliquot contains a sufficient number of embryos (in one or more vials or straws and based on the transfer success rate of the MMRRC facility) to transfer into one to three recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.

3 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.

4 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.