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Strain Name:
C57BL/6J-MtgxR6785Btlr/Mmmh
Stock Number:
044899-MU
Citation ID:
RRID:MMRRC_044899-MU
Other Names:
R6785 (G1)
Major Collection:

Strain Information

Fasl
Name: Fas ligand
Synonyms: Fas-L, CD178, APT1LG1, CD95L, Tnfsf6, Fasl
Type: Gene
Species: Mouse
Chromosome: 1
NCBI: 14103
Homologene: 533
Tead4
Name: TEA domain family member 4
Synonyms: Tefr1a, TEAD-4, Tcf13r1, TEF-3, ETFR-2a, Etfr2, Tefr, Tef3, Rtef1
Type: Gene
Species: Mouse
Chromosome: 6
NCBI: 21679
Homologene: 74463
Ppp6c
Name: protein phosphatase 6, catalytic subunit
Synonyms: 2310003C10Rik
Type: Gene
Species: Mouse
Chromosome: 2
NCBI: 67857
HGNC: HGNC:9323
Homologene: 68273
Ndnf
Name: neuron-derived neurotrophic factor
Synonyms: epidermacan, A930038C07Rik
Type: Gene
Species: Mouse
Chromosome: 6
NCBI: 68169
Homologene: 11593
Coa4
Name: cytochrome c oxidase assembly factor 4
Synonyms: 5330414O08Rik, Chchd8
Type: Gene
Species: Mouse
Chromosome: 7
NCBI: 68185
Homologene: 9567
Nfkb1
Name: nuclear factor of kappa light polypeptide gene enhancer in B cells 1, p105
Synonyms: NF kappaB1, p50 subunit of NF kappaB, p50/p105, p50, nuclear factor kappaB p50, NF-kappaB, NF-kappaB p50
Type: Gene
Species: Mouse
Chromosome: 3
NCBI: 18033
HGNC: HGNC:7794
Homologene: 2971
Fbxw8
Name: F-box and WD-40 domain protein 8
Synonyms: FBXO29, Fbx29, FBW8, FBW6, 4930438M06Rik
Type: Gene
Species: Mouse
Chromosome: 5
NCBI: 231672
Homologene: 17731
Genetic Alterations
ENU-induced transitions at the following base pair locations (GRCm38):
  • A to G, chromosome 1 at 91,322,221 bp
  • A to G, chromosome 1 at 117,801,069 bp
  • T to C, chromosome 1 at 129,430,907 bp
  • T to A, chromosome 1 at 161,781,835 bp
  • T to C, chromosome 1 at 162,709,101 bp
  • A to G, chromosome 2 at 22,953,467 bp
  • C to T, chromosome 2 at 32,387,027 bp
  • A to G, chromosome 2 at 36,376,842 bp
  • C to A, chromosome 2 at 36,376,951 bp
  • A to T, chromosome 2 at 39,197,581 bp
  • A to G, chromosome 2 at 69,183,927 bp
  • T to C, chromosome 2 at 76,711,495 bp
  • A to T, chromosome 2 at 76,747,944 bp
  • T to A, chromosome 2 at 86,594,421 bp
  • T to A, chromosome 2 at 127,229,165 bp
  • T to C, chromosome 2 at 155,560,685 bp
  • T to A, chromosome 3 at 36,165,452 bp
  • G to T, chromosome 3 at 59,319,244 bp
  • A to T, chromosome 3 at 92,850,193 bp
  • C to A, chromosome 3 at 95,326,401 bp
  • C to A, chromosome 3 at 135,615,303 bp
  • T to C, chromosome 4 at 59,049,066 bp
  • T to C, chromosome 4 at 137,508,398 bp
  • C to T, chromosome 4 at 149,982,790 bp
  • T to A, chromosome 4 at 155,454,024 bp
  • C to A, chromosome 5 at 99,978,424 bp
  • T to C, chromosome 5 at 118,092,689 bp
  • T to C, chromosome 5 at 121,609,845 bp
  • C to A, chromosome 6 at 65,703,063 bp
  • T to A, chromosome 6 at 128,242,481 bp
  • A to G, chromosome 7 at 3,685,836 bp
  • T to A, chromosome 7 at 8,908,204 bp
  • A to G, chromosome 7 at 23,866,137 bp
  • G to T, chromosome 7 at 29,064,874 bp
  • G to A, chromosome 7 at 38,222,064 bp
  • A to G, chromosome 7 at 45,060,225 bp
  • A to G, chromosome 7 at 82,522,004 bp
  • G to A, chromosome 7 at 100,539,253 bp
  • A to G, chromosome 7 at 109,810,617 bp
  • A to G, chromosome 7 at 142,274,108 bp
  • C to T, chromosome 8 at 13,370,485 bp
  • G to T, chromosome 8 at 13,377,233 bp
  • T to C, chromosome 8 at 95,095,642 bp
  • C to A, chromosome 8 at 111,124,611 bp
  • T to C, chromosome 8 at 126,394,459 bp
  • TCCC to TCC, chromosome 9 at 66,501,188 bp
  • T to C, chromosome 10 at 43,999,171 bp
  • T to C, chromosome 10 at 79,544,713 bp
  • A to G, chromosome 11 at 60,188,794 bp
  • T to C, chromosome 11 at 72,447,566 bp
  • A to T, chromosome 11 at 83,214,601 bp
  • A to C, chromosome 11 at 106,533,950 bp
  • T to C, chromosome 11 at 115,441,201 bp
  • A to G, chromosome 12 at 11,262,530 bp
  • G to A, chromosome 12 at 110,629,679 bp
  • C to T, chromosome 14 at 30,912,615 bp
  • T to A, chromosome 14 at 55,650,475 bp
  • A to T, chromosome 15 at 89,352,033 bp
  • A to T, chromosome 15 at 90,935,730 bp
  • T to G, chromosome 17 at 25,991,718 bp
  • T to C, chromosome 17 at 36,642,233 bp
  • A to G, chromosome 19 at 40,568,344 bp
Phenotype
G1 male heterozygous mice are viable and fertile. The G1 mice were not characterized prior to archiving.
Strain GQC Summary
Gene Specific Genotyping:

To request gene-specific and other genotyping services for a strain, please contact the distribution MMRRC Center for more information.

Background Genetic Quality:

The MMRRC has developed a Genetic Quality Control pipeline using the MiniMUGA array to provide additional information to identify and validate genetic backgrounds of MMRRC strains. For more information on whether genetic background data is available, please contact MMRRC_GeneticQC@med.unc.edu. Note: that MiniMUGA genetic background data is not available on all strains, but can be ordered if desired.

Suggested Control Mice
Littermates of all relevant genotypes.
Donor
Bruce Beutler, M.D., University of Texas Southwestern Medical Center.
Primary Reference
MUTAGENETIX record for R6785 (G1). B. Beutler and colleagues, Center for the Genetics of Host Defense, UT Southwestern, Dallas, TX.
Strain Development
C57BL/6J mice were injected with ENU, resulting in G0 mice that were bred back to C57BL/6J. Sperm was archived from G1 male mice that produced viable G2 mice.


Disclaimer: If MMRRC Strain Genetic Quality Control (GQC; based on MiniMUGA genotyping and analysis) has been completed for this strain, the information might differ from the genetic background information provided by the submitter. MiniMUGA genetic analysis is done on a strain’s tissue samples taken when archived by or ordered from the assigned MMRRC Center.

Colony and Husbandry Information

Colony Surveillance Program and Current Health Reports

Mice recovered from a cryo-archive will have health surveillance performed on recipient females. Health reports will be provided prior to shipment. If you require additional health status information, please email mmrrc@missouri.edu.
Coat Color
Black
MMRRC Breeding System
Random intra-strain mating
Breeding Scheme(s)
When maintaining a live colony, heterozygous mice may be bred together, bred with wild-type siblings, or bred with C57BL/6J inbred mice.
Overall Breeding Performance
Undetermined; during development, mating was done with heterozygous males; viability, fertility and other breeding statistics for heterozygous females and homozygotes are unverified, but presumably would be similar to the C57BL/6J background strain
Average litter size
Undetermined
Recommended wean age
3 weeks

Order Information

Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.

Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.

The donor or their institution limits the distribution to non-profit institutions only.

Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.

Click button to Request this one strain. (Use the MMRRC Catalog Search to request more than one strain.)
MMRRC Item # Description Distribution Fee / Unit (US $)
*Shipping & Handling not included*
Units Notes
044899-MU-RESUS Litter recovered from cryo-archive $5,475.00 / Non-Profit Litter Recovered litter4; additional fees for any special requests.
Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

1 The distribution fee covers the expense of rederiving mice from a live mouse; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

2 An aliquot contains a sufficient number of embryos (in one or more vials or straws and based on the transfer success rate of the MMRRC facility) to transfer into one to three recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.

3 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.

4 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.