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Strain Name:
C57BL/6J-MtgxR6830Btlr/Mmmh
Stock Number:
044940-MU
Citation ID:
RRID:MMRRC_044940-MU
Other Names:
R6830 (G1)
Major Collection:

Strain Information

Fgb
Name: fibrinogen beta chain
Synonyms: 2510049G14Rik
Type: Gene
Species: Mouse
Chromosome: 3
NCBI: 110135
HGNC: HGNC:3662
Homologene: 3772
Ldb2
Name: LIM domain binding 2
Synonyms: CLIM1, Ldb3, CLP-36, CLIM-1b, CLIM-1a
Type: Gene
Species: Mouse
Chromosome: 5
NCBI: 16826
HGNC: HGNC:6533
Homologene: 989
Plekhm1
Name: pleckstrin homology domain containing, family M (with RUN domain) member 1
Synonyms: D330036J23Rik, AP162, B2
Type: Gene
Species: Mouse
Chromosome: 11
NCBI: 353047
Homologene: 8871
Dock9
Name: dedicator of cytokinesis 9
Synonyms: B230309H04Rik, D14Wsu89e, Zizimin1
Type: Gene
Species: Mouse
Chromosome: 14
NCBI: 105445
Homologene: 41026
Prep
Name: prolyl endopeptidase
Synonyms: prolyl oligopeptidase, D10Wsu136e, Pop
Type: Gene
Species: Mouse
Chromosome: 10
NCBI: 19072
VEGA: 10
HGNC: HGNC:9358
Homologene: 2042
Ano6
Name: anoctamin 6
Synonyms: 2900059G15Rik, F730003B03Rik, Tmem16f
Type: Gene
Species: Mouse
Chromosome: 15
NCBI: 105722
VEGA: 15
Homologene: 27888
Ttc27
Name: tetratricopeptide repeat domain 27
Synonyms: 2610511O17Rik
Type: Gene
Species: Mouse
Chromosome: 17
NCBI: 74196
VEGA: 17
Homologene: 41191
Genetic Alterations
ENU-induced transitions at the following base pair locations (GRCm38):
  • A to G, chromosome 1 at 44,056,730 bp
  • T to C, chromosome 1 at 87,387,023 bp
  • A to T, chromosome 1 at 138,072,255 bp
  • A to G, chromosome 1 at 154,413,974 bp
  • A to G, chromosome 1 at 163,076,366 bp
  • A to G, chromosome 2 at 28,505,208 bp
  • T to C, chromosome 2 at 66,568,029 bp
  • T to A, chromosome 2 at 83,939,684 bp
  • T to A, chromosome 2 at 140,452,682 bp
  • C to T, chromosome 2 at 164,734,258 bp
  • T to A, chromosome 3 at 38,981,817 bp
  • T to C, chromosome 3 at 83,045,025 bp
  • T to C, chromosome 3 at 100,993,696 bp
  • T to C, chromosome 3 at 146,650,961 bp
  • T to C, chromosome 3 at 159,614,168 bp
  • T to C, chromosome 4 at 15,924,355 bp
  • T to C, chromosome 4 at 108,021,417 bp
  • A to T, chromosome 5 at 14,681,099 bp
  • T to A, chromosome 5 at 31,507,488 bp
  • T to A, chromosome 5 at 34,834,326 bp
  • A to T, chromosome 5 at 43,609,094 bp
  • T to A, chromosome 5 at 44,541,857 bp
  • T to C, chromosome 5 at 88,378,097 bp
  • A to G, chromosome 6 at 78,407,642 bp
  • A to T, chromosome 6 at 146,766,771 bp
  • A to G, chromosome 7 at 29,776,486 bp
  • A to T, chromosome 7 at 42,043,747 bp
  • T to A, chromosome 7 at 104,580,240 bp
  • A to T, chromosome 7 at 141,898,714 bp
  • A to T, chromosome 8 at 22,022,365 bp
  • G to T, chromosome 8 at 53,599,479 bp
  • A to G, chromosome 8 at 122,456,473 bp
  • A to C, chromosome 10 at 18,664,889 bp
  • T to A, chromosome 10 at 45,097,501 bp
  • A to G, chromosome 11 at 87,868,626 bp
  • A to T, chromosome 11 at 103,376,889 bp
  • G to A, chromosome 12 at 66,723,001 bp
  • G to A, chromosome 14 at 121,622,918 bp
  • T to C, chromosome 15 at 8,176,184 bp
  • C to A, chromosome 15 at 13,044,774 bp
  • T to A, chromosome 15 at 83,544,208 bp
  • A to G, chromosome 15 at 95,894,461 bp
  • T to C, chromosome 16 at 4,879,720 bp
  • A to G, chromosome 16 at 44,731,357 bp
  • C to T, chromosome 16 at 97,951,961 bp
  • A to G, chromosome 17 at 29,000,007 bp
  • T to C, chromosome 17 at 36,888,850 bp
  • G to A, chromosome 17 at 57,053,804 bp
  • T to A, chromosome 17 at 74,856,555 bp
  • C to T, chromosome 18 at 22,525,388 bp
  • T to A, chromosome 18 at 36,738,353 bp
  • C to T, chromosome 18 at 49,921,024 bp
  • A to G, chromosome 19 at 40,845,192 bp
Phenotype
G1 male heterozygous mice are viable and fertile. The G1 mice were not characterized prior to archiving.
Strain GQC Summary
Gene Specific Genotyping:

To request gene-specific and other genotyping services for a strain, please contact the distribution MMRRC Center for more information.

Background Genetic Quality:

The MMRRC has developed a Genetic Quality Control pipeline using the MiniMUGA array to provide additional information to identify and validate genetic backgrounds of MMRRC strains. For more information on whether genetic background data is available, please contact MMRRC_GeneticQC@med.unc.edu. Note: that MiniMUGA genetic background data is not available on all strains, but can be ordered if desired.

Suggested Control Mice
Littermates of all relevant genotypes.
Donor
Bruce Beutler, M.D., University of Texas Southwestern Medical Center.
Primary Reference
MUTAGENETIX record for R6830 (G1). B. Beutler and colleagues, Center for the Genetics of Host Defense, UT Southwestern, Dallas, TX.
Strain Development
C57BL/6J mice were injected with ENU, resulting in G0 mice that were bred back to C57BL/6J. Sperm was archived from G1 male mice that produced viable G2 mice.


Disclaimer: If MMRRC Strain Genetic Quality Control (GQC; based on MiniMUGA genotyping and analysis) has been completed for this strain, the information might differ from the genetic background information provided by the submitter. MiniMUGA genetic analysis is done on a strain’s tissue samples taken when archived by or ordered from the assigned MMRRC Center.

Colony and Husbandry Information

Colony Surveillance Program and Current Health Reports

Mice recovered from a cryo-archive will have health surveillance performed on recipient females. Health reports will be provided prior to shipment. If you require additional health status information, please email mmrrc@missouri.edu.
Coat Color
Black
MMRRC Breeding System
Random intra-strain mating
Breeding Scheme(s)
When maintaining a live colony, heterozygous mice may be bred together, bred with wild-type siblings, or bred with C57BL/6J inbred mice.
Overall Breeding Performance
Undetermined; during development, mating was done with heterozygous males; viability, fertility and other breeding statistics for heterozygous females and homozygotes are unverified, but presumably would be similar to the C57BL/6J background strain
Average litter size
Undetermined
Recommended wean age
3 weeks

Order Information

Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.

Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.

The donor or their institution limits the distribution to non-profit institutions only.

Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.

Click button to Request this one strain. (Use the MMRRC Catalog Search to request more than one strain.)
MMRRC Item # Description Distribution Fee / Unit (US $)
*Shipping & Handling not included*
Units Notes
044940-MU-RESUS Litter recovered from cryo-archive $5,475.00 / Non-Profit Litter Recovered litter4; additional fees for any special requests.
Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

1 The distribution fee covers the expense of rederiving mice from a live mouse; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

2 An aliquot contains a sufficient number of embryos (in one or more vials or straws and based on the transfer success rate of the MMRRC facility) to transfer into one to three recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.

3 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.

4 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.