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Strain Detail Sheet

Strain Name:
C57BL/6J-MtgxR6853Btlr/Mmmh
Stock Number:
044956-MU
Citation ID:
RRID:MMRRC_044956-MU
Other Names:
R6853 (G1)
Major Collection:
Beutler Mutagenetix

Strain Information

Gene Details

Fga
Name: fibrinogen alpha chain
Synonyms: Fib, ENSMUSG00000059807
Type: Gene
Species: Mouse
Chromosome: 3
NCBI: 14161
HGNC: HGNC:3661
Homologene: 428

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Inpp5d
Name: inositol polyphosphate-5-phosphatase D
Synonyms: SHIP, Src homology 2 domain-containing inositol-5-phosphatase, s-SHIP, SHIP-1, SHIP1
Type: Gene
Species: Mouse
Chromosome: 1
NCBI: 16331
HGNC: HGNC:6079
Homologene: 4046

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Google
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Thop1
Name: thimet oligopeptidase 1
Synonyms: EP24.15
Type: Gene
Species: Mouse
Chromosome: 10
NCBI: 50492
Homologene: 55726

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Lhx9
Name: LIM homeobox protein 9
Synonyms: LH2B, Lhx9 alpha, 3110009O07Rik
Type: Gene
Species: Mouse
Chromosome: 1
NCBI: 16876
Homologene: 7816

Additional Resources
IMPC
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Efr3a
Name: EFR3 homolog A
Synonyms: A130089M23Rik, D030063F01Rik, C920006C10Rik
Type: Gene
Species: Mouse
Chromosome: 15
NCBI: 76740
Homologene: 44222

Additional Resources
IMPC
Google
Entrez
PubMed
IMSR
Gene Ontology
BioGPS
Gene Cloud
IGTC
Mouse Phenome DB
Mouse Mine
KOMP Phenotyping
Monarch
GeneCards
ClinGen
Lgals9
Name: lectin, galactose binding, soluble 9
Synonyms: LGALS35, galectin-9, gal-9, Lgals5
Type: Gene
Species: Mouse
Chromosome: 11
NCBI: 16859
Homologene: 134662

Additional Resources
IMPC
Google
Entrez
PubMed
IMSR
Gene Ontology
BioGPS
Gene Cloud
IGTC
Mouse Phenome DB
Mouse Mine
KOMP Phenotyping
Monarch
GeneCards
ClinGen
Pabpc4
Name: poly(A) binding protein, cytoplasmic 4
Type: Gene
Species: Mouse
Chromosome: 4
NCBI: 230721
HGNC: HGNC:8557
Homologene: 37855

Additional Resources
IMPC
Google
Entrez
PubMed
IMSR
Gene Ontology
BioGPS
Gene Cloud
IGTC
Mouse Phenome DB
Mouse Mine
KOMP Phenotyping
Monarch
GeneCards
ClinGen
Genetic Alterations
ENU-induced transitions at the following base pair locations (GRCm38):
  • T to A, chromosome 1 at 87,681,680 bp
  • T to G, chromosome 1 at 93,039,802 bp
  • T to C, chromosome 1 at 93,570,016 bp
  • A to C, chromosome 1 at 117,986,144 bp
  • ACC to ACCC, chromosome 1 at 138,841,806 bp
  • T to G, chromosome 1 at 188,911,237 bp
  • T to C, chromosome 2 at 10,115,266 bp
  • T to A, chromosome 2 at 35,129,821 bp
  • T to C, chromosome 2 at 101,642,221 bp
  • C to T, chromosome 2 at 104,241,155 bp
  • A to G, chromosome 2 at 110,624,194 bp
  • T to A, chromosome 2 at 164,837,364 bp
  • A to G, chromosome 3 at 72,891,426 bp
  • A to G, chromosome 3 at 83,030,912 bp
  • T to C, chromosome 3 at 100,013,235 bp
  • T to C, chromosome 4 at 123,294,743 bp
  • C to A, chromosome 4 at 123,342,683 bp
  • T to C, chromosome 4 at 149,982,462 bp
  • T to C, chromosome 5 at 30,388,239 bp
  • A to T, chromosome 5 at 109,092,905 bp
  • T to A, chromosome 5 at 120,907,428 bp
  • A to G, chromosome 5 at 148,107,011 bp
  • G to A, chromosome 6 at 72,380,441 bp
  • A to G, chromosome 6 at 83,053,831 bp
  • T to G, chromosome 6 at 97,168,849 bp
  • CAAAGTAA to CAAAGTAAAGTAA, chromosome 6 at 113,399,157 bp
  • C to T, chromosome 7 at 13,801,487 bp
  • T to C, chromosome 7 at 19,760,406 bp
  • T to C, chromosome 7 at 25,518,136 bp
  • C to T, chromosome 7 at 92,859,565 bp
  • G to A, chromosome 7 at 96,837,295 bp
  • G to A, chromosome 7 at 105,703,728 bp
  • T to G, chromosome 9 at 19,496,806 bp
  • G to A, chromosome 9 at 36,741,727 bp
  • G to A, chromosome 9 at 44,818,407 bp
  • A to G, chromosome 10 at 19,003,751 bp
  • T to C, chromosome 10 at 81,075,661 bp
  • T to C, chromosome 11 at 78,232,830 bp
  • A to T, chromosome 11 at 78,966,006 bp
  • A to G, chromosome 11 at 113,780,929 bp
  • T to G, chromosome 13 at 70,603,302 bp
  • A to G, chromosome 13 at 92,312,572 bp
  • G to T, chromosome 15 at 12,817,945 bp
  • A to T, chromosome 15 at 64,817,865 bp
  • A to T, chromosome 15 at 64,817,867 bp
  • G to A, chromosome 15 at 65,829,830 bp
  • A to G, chromosome 16 at 29,321,662 bp
  • C to T, chromosome 16 at 58,889,758 bp
  • T to A, chromosome 16 at 58,889,759 bp
  • T to A, chromosome 16 at 84,860,499 bp
  • A to T, chromosome 17 at 19,065,801 bp
  • A to T, chromosome 17 at 31,854,206 bp
  • T to C, chromosome 17 at 35,428,359 bp
  • G to A, chromosome 17 at 37,111,508 bp
  • C to T, chromosome 17 at 65,852,756 bp
  • G to A, chromosome 17 at 68,777,920 bp
  • A to T, chromosome 17 at 71,436,743 bp
  • C to A, chromosome 17 at 81,065,030 bp
  • A to T, chromosome 18 at 22,109,876 bp
  • A to G, chromosome 18 at 24,599,319 bp
  • T to A, chromosome 19 at 13,529,931 bp
  • T to A, chromosome 19 at 39,438,304 bp
  • C to T, chromosome 19 at 40,183,920 bp
  • A to T, chromosome 19 at 60,529,466 bp

Strain Description

Phenotype
G1 male heterozygous mice are viable and fertile. The G1 mice were not characterized prior to archiving.
Strain GQC Summary
The MMRRC Centers have developed a Strain GQC pipeline using MiniMUGA array genotyping to provide additional information on strain backgrounds for MMRRC strains. For more information on whether data may be available, or to request genotyping for a strain of interest, please contact MMRRC_GeneticQC@med.unc.edu. Older strains may not have this information available.
Suggested Control Mice
Littermates of all relevant genotypes.

Strain Origin

Donor
Bruce Beutler, M.D., University of Texas Southwestern Medical Center.
Primary Reference
MUTAGENETIX record for R6853 (G1). B. Beutler and colleagues, Center for the Genetics of Host Defense, UT Southwestern, Dallas, TX.
Strain Development
C57BL/6J mice were injected with ENU, resulting in G0 mice that were bred back to C57BL/6J. Sperm was archived from G1 male mice that produced viable G2 mice.


Disclaimer: If MMRRC Strain Genetic Quality Control (GQC; based on MiniMUGA genotyping and analysis) has been completed for this strain, the information might differ from the genetic background information provided by the submitter. MiniMUGA genetic analysis is done on a strain’s tissue samples taken when archived by or ordered from the assigned MMRRC Center.

Colony and Husbandry Information

Health Status Report

Colony Surveillance Program and Current Health Reports

Mice recovered from a cryo-archive will have health surveillance performed on recipient females. Health reports will be provided prior to shipment. If you require additional health status information, please email mmrrc@missouri.edu.

Appearance

Coat Color
Black

Breeding

MMRRC Breeding System
Random intra-strain mating
Breeding Scheme(s)
When maintaining a live colony, heterozygous mice may be bred together, bred with wild-type siblings, or bred with C57BL/6J inbred mice.
Overall Breeding Performance
Undetermined; during development, mating was done with heterozygous males; viability, fertility and other breeding statistics for heterozygous females and homozygotes are unverified, but presumably would be similar to the C57BL/6J background strain

Reproductive Statistics

Average litter size
Undetermined
Recommended wean age
3 weeks

Order Request Information

Availability Level

Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.

Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

Conditions of Distribution

Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.

The donor or their institution limits the distribution to non-profit institutions only.

Fees

Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.

Click button to Request this one strain. (Use the MMRRC Catalog Search to request more than one strain.)
MMRRC Item # Description Distribution Fee / Unit (US $)
*Shipping & Handling not included*		 Units Notes
044956-MU-RESUS Litter recovered from cryo-archive $5,475.00 / Non-Profit Litter Recovered litter4; additional fees for any special requests.
Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

1 The distribution fee covers the expense of rederiving mice from a live mouse; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

2 An aliquot contains a sufficient number of embryos (in one or more vials or straws and based on the transfer success rate of the MMRRC facility) to transfer into one to three recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.

3 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.

4 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.