Loading Mouse GIF
Loading...

Strain Name:
C57BL/6J-MtgxR6857Btlr/Mmmh
Stock Number:
044959-MU
Citation ID:
RRID:MMRRC_044959-MU
Other Names:
R6857 (G1)
Major Collection:

Strain Information

Tagln3
Name: transgelin 3
Synonyms: 2900005O10Rik, 2700038H05Rik, Np25
Type: Gene
Species: Mouse
Chromosome: 16
NCBI: 56370
Homologene: 22337
Plxnd1
Name: plexin D1
Synonyms: 6230425C21Rik, b2b553Clo, b2b1863Clo
Type: Gene
Species: Mouse
Chromosome: 6
NCBI: 67784
HGNC: HGNC:9107
Homologene: 22866
Nek7
Name: NIMA (never in mitosis gene a)-related expressed kinase 7
Synonyms: 2810460C19Rik
Type: Gene
Species: Mouse
Chromosome: 1
NCBI: 59125
Homologene: 69340
Rgs12
Name: regulator of G-protein signaling 12
Synonyms: 4632412M04Rik, 1200016K18Rik
Type: Gene
Species: Mouse
Chromosome: 5
NCBI: 71729
HGNC: HGNC:9994
Homologene: 2195
Mtmr12
Name: myotubularin related protein 12
Synonyms: C730015A02Rik, Pip3ap
Type: Gene
Species: Mouse
Chromosome: 15
NCBI: 268783
Homologene: 10403
Tcf3
Name: transcription factor 3
Synonyms: ALF2, E2A, E47, E12, Pan2, Pan1, A1, Tcfe2a, bHLHb21
Type: Gene
Species: Mouse
Chromosome: 10
NCBI: 21423
Homologene: 2408
Cdk5rap2
Name: CDK5 regulatory subunit associated protein 2
Synonyms: 2900018K03Rik, an
Type: Gene
Species: Mouse
Chromosome: 4
NCBI: 214444
Homologene: 49533
Genetic Alterations
ENU-induced transitions at the following base pair locations (GRCm38):
  • T to C, chromosome 1 at 21,229,545 bp
  • G to C, chromosome 1 at 24,014,789 bp
  • A to T, chromosome 1 at 117,801,288 bp
  • C to T, chromosome 1 at 138,515,682 bp
  • A to G, chromosome 2 at 34,728,377 bp
  • T to A, chromosome 2 at 76,917,931 bp
  • A to T, chromosome 2 at 86,594,264 bp
  • A to T, chromosome 2 at 87,753,487 bp
  • G to T, chromosome 2 at 150,192,062 bp
  • T to G, chromosome 3 at 89,997,452 bp
  • T to A, chromosome 3 at 127,581,447 bp
  • A to G, chromosome 4 at 9,449,433 bp
  • T to A, chromosome 4 at 58,814,065 bp
  • C to A, chromosome 4 at 70,245,396 bp
  • T to C, chromosome 4 at 118,963,826 bp
  • G to A, chromosome 4 at 139,486,051 bp
  • A to G, chromosome 4 at 155,843,136 bp
  • A to T, chromosome 5 at 35,030,022 bp
  • T to A, chromosome 5 at 43,906,198 bp
  • T to C, chromosome 5 at 69,296,702 bp
  • C to G, chromosome 5 at 87,240,846 bp
  • G to T, chromosome 6 at 30,863,598 bp
  • T to C, chromosome 6 at 54,629,935 bp
  • G to T, chromosome 6 at 115,993,763 bp
  • C to A, chromosome 6 at 146,397,019 bp
  • A to T, chromosome 7 at 13,178,331 bp
  • A to G, chromosome 8 at 27,045,426 bp
  • C to A, chromosome 8 at 62,061,017 bp
  • A to G, chromosome 8 at 85,007,823 bp
  • T to C, chromosome 9 at 38,607,011 bp
  • T to G, chromosome 9 at 44,985,604 bp
  • G to T, chromosome 9 at 61,976,088 bp
  • G to T, chromosome 9 at 74,155,041 bp
  • G to A, chromosome 9 at 102,542,432 bp
  • T to C, chromosome 10 at 7,920,413 bp
  • T to C, chromosome 10 at 80,416,899 bp
  • A to T, chromosome 11 at 62,281,129 bp
  • T to C, chromosome 11 at 67,249,885 bp
  • A to G, chromosome 11 at 82,913,212 bp
  • T to A, chromosome 11 at 87,869,955 bp
  • A to G, chromosome 11 at 105,910,288 bp
  • A to G, chromosome 11 at 110,219,688 bp
  • T to G, chromosome 12 at 34,393,363 bp
  • T to C, chromosome 12 at 81,419,949 bp
  • T to C, chromosome 12 at 104,389,326 bp
  • T to C, chromosome 12 at 110,658,547 bp
  • A to T, chromosome 13 at 66,432,102 bp
  • A to T, chromosome 13 at 113,319,958 bp
  • T to A, chromosome 15 at 12,263,832 bp
  • T to C, chromosome 15 at 102,223,465 bp
  • G to T, chromosome 16 at 45,724,236 bp
  • T to A, chromosome 17 at 7,772,170 bp
  • T to C, chromosome 17 at 15,544,256 bp
  • C to T, chromosome 17 at 20,540,670 bp
  • A to T, chromosome 18 at 49,864,835 bp
  • G to T, chromosome 19 at 6,393,757 bp
  • C to T, chromosome 19 at 9,037,168 bp
  • T to C, chromosome 19 at 40,770,865 bp
Phenotype
G1 male heterozygous mice are viable and fertile. The G1 mice were not characterized prior to archiving.
Strain GQC Summary
Gene Specific Genotyping:

To request gene-specific and other genotyping services for a strain, please contact the distribution MMRRC Center for more information.

Background Genetic Quality:

The MMRRC has developed a Genetic Quality Control pipeline using the MiniMUGA array to provide additional information to identify and validate genetic backgrounds of MMRRC strains. For more information on whether genetic background data is available, please contact MMRRC_GeneticQC@med.unc.edu. Note: that MiniMUGA genetic background data is not available on all strains, but can be ordered if desired.

Suggested Control Mice
Littermates of all relevant genotypes.
Donor
Bruce Beutler, M.D., University of Texas Southwestern Medical Center.
Primary Reference
MUTAGENETIX record for R6857 (G1). B. Beutler and colleagues, Center for the Genetics of Host Defense, UT Southwestern, Dallas, TX.
Strain Development
C57BL/6J mice were injected with ENU, resulting in G0 mice that were bred back to C57BL/6J. Sperm was archived from G1 male mice that produced viable G2 mice.


Disclaimer: If MMRRC Strain Genetic Quality Control (GQC; based on MiniMUGA genotyping and analysis) has been completed for this strain, the information might differ from the genetic background information provided by the submitter. MiniMUGA genetic analysis is done on a strain’s tissue samples taken when archived by or ordered from the assigned MMRRC Center.

Colony and Husbandry Information

Colony Surveillance Program and Current Health Reports

Mice recovered from a cryo-archive will have health surveillance performed on recipient females. Health reports will be provided prior to shipment. If you require additional health status information, please email mmrrc@missouri.edu.
Coat Color
Black
MMRRC Breeding System
Random intra-strain mating
Breeding Scheme(s)
When maintaining a live colony, heterozygous mice may be bred together, bred with wild-type siblings, or bred with C57BL/6J inbred mice.
Overall Breeding Performance
Undetermined; during development, mating was done with heterozygous males; viability, fertility and other breeding statistics for heterozygous females and homozygotes are unverified, but presumably would be similar to the C57BL/6J background strain
Average litter size
Undetermined
Recommended wean age
3 weeks

Order Information

Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.

Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.

The donor or their institution limits the distribution to non-profit institutions only.

Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.

Click button to Request this one strain. (Use the MMRRC Catalog Search to request more than one strain.)
MMRRC Item # Description Distribution Fee / Unit (US $)
*Shipping & Handling not included*
Units Notes
044959-MU-RESUS Litter recovered from cryo-archive $5,475.00 / Non-Profit Litter Recovered litter4; additional fees for any special requests.
Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

1 The distribution fee covers the expense of rederiving mice from a live mouse; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

2 An aliquot contains a sufficient number of embryos (in one or more vials or straws and based on the transfer success rate of the MMRRC facility) to transfer into one to three recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.

3 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.

4 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.