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Strain Name:
C57BL/6J-MtgxR6891Btlr/Mmmh
Stock Number:
044985-MU
Citation ID:
RRID:MMRRC_044985-MU
Other Names:
R6891 (G1)
Major Collection:

Strain Information

Polr3e
Name: polymerase (RNA) III (DNA directed) polypeptide E
Synonyms: RPC5, Sin
Type: Gene
Species: Mouse
Chromosome: 7
NCBI: 26939
Homologene: 14052
Pald1
Name: phosphatase domain containing, paladin 1
Synonyms: paladin, X99384
Type: Gene
Species: Mouse
Chromosome: 10
NCBI: 27355
VEGA: 10
Homologene: 8453
Hgf
Name: hepatocyte growth factor
Synonyms: scatter factor, NK1, SF/HGF, HGF/SF, NK2, C230052L06Rik
Type: Gene
Species: Mouse
Chromosome: 5
NCBI: 15234
HGNC: HGNC:4893
Homologene: 503
Gjd2
Name: gap junction protein, delta 2
Synonyms: connexin36, Cx36, Gja9
Type: Gene
Species: Mouse
Chromosome: 2
NCBI: 14617
Homologene: 7734
Mfn1
Name: mitofusin 1
Synonyms: 6330416C07Rik, 2310002F04Rik, D3Ertd265e, HR2
Type: Gene
Species: Mouse
Chromosome: 3
NCBI: 67414
Homologene: 11481
Gon4l
Name: gon-4 like
Synonyms: 1500041I23Rik, 2610100B20Rik
Type: Gene
Species: Mouse
Chromosome: 3
NCBI: 76022
Homologene: 13002
Mapkap1
Name: mitogen-activated protein kinase associated protein 1
Synonyms: D230039K05Rik, Sin1
Type: Gene
Species: Mouse
Chromosome: 2
NCBI: 227743
Homologene: 11473
Genetic Alterations
ENU-induced transitions at the following base pair locations (GRCm38):
  • C to A, chromosome 1 at 42,905,413 bp
  • G to T, chromosome 1 at 175,706,068 bp
  • T to A, chromosome 2 at 20,850,331 bp
  • A to G, chromosome 2 at 23,195,389 bp
  • T to C, chromosome 2 at 34,563,141 bp
  • C to A, chromosome 2 at 85,488,805 bp
  • T to G, chromosome 2 at 85,488,813 bp
  • A to G, chromosome 2 at 91,685,791 bp
  • T to G, chromosome 2 at 92,613,743 bp
  • C to T, chromosome 2 at 107,296,307 bp
  • G to A, chromosome 2 at 114,013,094 bp
  • A to T, chromosome 2 at 148,684,769 bp
  • C to T, chromosome 2 at 165,382,724 bp
  • C to A, chromosome 3 at 19,135,041 bp
  • T to C, chromosome 3 at 32,577,103 bp
  • T to C, chromosome 3 at 63,698,083 bp
  • A to T, chromosome 3 at 75,333,283 bp
  • A to G, chromosome 3 at 88,858,866 bp
  • G to A, chromosome 3 at 96,598,019 bp
  • A to T, chromosome 4 at 41,394,168 bp
  • G to T, chromosome 4 at 126,846,438 bp
  • A to G, chromosome 4 at 134,081,837 bp
  • A to T, chromosome 4 at 141,755,881 bp
  • G to A, chromosome 5 at 16,604,922 bp
  • G to T, chromosome 5 at 30,895,178 bp
  • T to A, chromosome 5 at 31,523,021 bp
  • C to A, chromosome 5 at 94,218,119 bp
  • T to A, chromosome 5 at 106,917,374 bp
  • C to T, chromosome 5 at 109,001,979 bp
  • C to T, chromosome 5 at 114,349,612 bp
  • GAT to GATCAT, chromosome 6 at 4,756,449 bp
  • A to T, chromosome 6 at 123,283,606 bp
  • A to G, chromosome 7 at 4,851,488 bp
  • T to C, chromosome 7 at 104,841,100 bp
  • A to G, chromosome 7 at 104,959,985 bp
  • A to G, chromosome 7 at 120,944,650 bp
  • T to A, chromosome 7 at 126,582,803 bp
  • T to A, chromosome 7 at 145,322,350 bp
  • G to A, chromosome 8 at 4,523,731 bp
  • A to G, chromosome 8 at 106,264,668 bp
  • C to A, chromosome 8 at 129,026,638 bp
  • A to G, chromosome 9 at 54,480,380 bp
  • C to T, chromosome 9 at 107,929,147 bp
  • T to C, chromosome 9 at 111,025,113 bp
  • A to G, chromosome 10 at 19,011,669 bp
  • A to T, chromosome 10 at 27,328,072 bp
  • G to C, chromosome 10 at 27,328,082 bp
  • A to G, chromosome 10 at 61,348,532 bp
  • A to G, chromosome 10 at 118,148,032 bp
  • C to T, chromosome 11 at 78,075,733 bp
  • T to C, chromosome 11 at 117,812,673 bp
  • A to T, chromosome 12 at 13,236,095 bp
  • A to G, chromosome 12 at 40,779,136 bp
  • A to G, chromosome 12 at 84,354,780 bp
  • A to T, chromosome 12 at 105,809,691 bp
  • T to C, chromosome 13 at 23,482,844 bp
  • A to G, chromosome 13 at 24,141,723 bp
  • T to A, chromosome 14 at 17,981,899 bp
  • A to G, chromosome 14 at 21,669,036 bp
  • A to T, chromosome 14 at 37,097,377 bp
  • T to C, chromosome 14 at 121,476,030 bp
  • A to T, chromosome 15 at 77,955,709 bp
  • A to G, chromosome 15 at 99,082,688 bp
  • T to C, chromosome 15 at 99,325,873 bp
  • A to G, chromosome 15 at 103,357,543 bp
  • T to C, chromosome 17 at 24,896,855 bp
  • C to T, chromosome 17 at 28,057,424 bp
  • T to A, chromosome 17 at 31,622,457 bp
  • A to G, chromosome 17 at 37,795,504 bp
  • T to A, chromosome 18 at 42,578,920 bp
  • A to T, chromosome 19 at 45,151,318 bp
  • A to T, chromosome 19 at 50,225,119 bp
  • T to C, chromosome 19 at 53,988,117 bp
Phenotype
G1 male heterozygous mice are viable and fertile. The G1 mice were not characterized prior to archiving.
Strain GQC Summary
Gene Specific Genotyping:

To request gene-specific and other genotyping services for a strain, please contact the distribution MMRRC Center for more information.

Background Genetic Quality:

The MMRRC has developed a Genetic Quality Control pipeline using the MiniMUGA array to provide additional information to identify and validate genetic backgrounds of MMRRC strains. For more information on whether genetic background data is available, please contact MMRRC_GeneticQC@med.unc.edu. Note: that MiniMUGA genetic background data is not available on all strains, but can be ordered if desired.

Suggested Control Mice
Littermates of all relevant genotypes.
Donor
Bruce Beutler, M.D., University of Texas Southwestern Medical Center.
Primary Reference
MUTAGENETIX record for R6891 (G1). B. Beutler and colleagues, Center for the Genetics of Host Defense, UT Southwestern, Dallas, TX.
Strain Development
C57BL/6J mice were injected with ENU, resulting in G0 mice that were bred back to C57BL/6J. Sperm was archived from G1 male mice that produced viable G2 mice.


Disclaimer: If MMRRC Strain Genetic Quality Control (GQC; based on MiniMUGA genotyping and analysis) has been completed for this strain, the information might differ from the genetic background information provided by the submitter. MiniMUGA genetic analysis is done on a strain’s tissue samples taken when archived by or ordered from the assigned MMRRC Center.

Colony and Husbandry Information

Colony Surveillance Program and Current Health Reports

Mice recovered from a cryo-archive will have health surveillance performed on recipient females. Health reports will be provided prior to shipment. If you require additional health status information, please email mmrrc@missouri.edu.
Coat Color
Black
MMRRC Breeding System
Random intra-strain mating
Breeding Scheme(s)
When maintaining a live colony, heterozygous mice may be bred together, bred with wild-type siblings, or bred with C57BL/6J inbred mice.
Overall Breeding Performance
Undetermined; during development, mating was done with heterozygous males; viability, fertility and other breeding statistics for heterozygous females and homozygotes are unverified, but presumably would be similar to the C57BL/6J background strain
Average litter size
Undetermined
Recommended wean age
3 weeks

Order Information

Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.

Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.

The donor or their institution limits the distribution to non-profit institutions only.

Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.

Click button to Request this one strain. (Use the MMRRC Catalog Search to request more than one strain.)
MMRRC Item # Description Distribution Fee / Unit (US $)
*Shipping & Handling not included*
Units Notes
044985-MU-RESUS Litter recovered from cryo-archive $5,475.00 / Non-Profit Litter Recovered litter4; additional fees for any special requests.
Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

1 The distribution fee covers the expense of rederiving mice from a live mouse; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

2 An aliquot contains a sufficient number of embryos (in one or more vials or straws and based on the transfer success rate of the MMRRC facility) to transfer into one to three recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.

3 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.

4 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.